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2022

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Abierto Inglés Background The exposure of breast cancer to extremely low frequency magnetic fields (ELF-MFs) results in various biological responses. Some studies have suggested a possible cancer-enhancing effect, while others showed a possible therapeutic role. This study investigated the effects of in vitro exposure to 50 Hz ELF-MF for up to 24 h on the viability and cellular response of MDA-MB-231 and MCF-7 breast cancer cell lines and MCF-10A breast cell line. Methods and results The breast cell lines were exposed to 50 Hz ELF-MF at flux densities of 0.1 mT and 1.0 mT and were examined 96 h after the beginning of ELF-MF exposure. The duration of 50 Hz ELF-MF exposure influenced the cell viability and proliferation of both the tumor and nontumorigenic breast cell lines. In particular, short-term exposure (4–8 h, 0.1 mT and 1.0 mT) led to an increase in viability in breast cancer cells, while long and high exposure (24 h, 1.0 mT) led to a decrease in viability and proliferation in all cell lines. Cancer and normal breast cells exhibited different responses to ELF-MF. Mitochondrial membrane potential and reactive oxygen species (ROS) production were altered after ELF-MF exposure, suggesting that the mitochondria are a probable target of ELF-MF in breast cells. Conclusions The viability of breast cells in vitro is influenced by ELF-MF exposure at magnetic flux densities compatible with the limits for the general population and for workplace exposures. The effects are apparent after 96 h and are related to the ELF-MF exposure time. metadata Elexpuru Zabaleta, Maria and Lazzarini, Raffaella and Tartaglione, Maria Fiorella and Piva, Francesco and Ciarapica, Veronica and Marinelli Busilacchi, Elena and Poloni, Antonella and Valentino, Matteo and Santarelli, Lory and Bracci, Massimo mail maria.elexpuru@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2022) A 50 Hz magnetic field influences the viability of breast cancer cells 96 h after exposure. Molecular Biology Reports. ISSN 0301-4851

Article Subjects > Biomedicine
Subjects > Nutrition
Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Abierto Inglés Background: The aim of this study was to relate adherence to the Mediterranean diet (MedDiet) to the prevalence of metabolic syndrome (MetS) in an elderly population from the north of Spain. Methods: We carried out an observational, descriptive, cross-sectional, and correlational study involving 556 non-institutionalised individuals aged 65 to 79 years. The MEDAS-14 questionnaire score was used to define the degree of adherence to the Mediterranean diet. The diagnosis of MetS was conducted using the International Diabetes Federation (IDF) criteria. Results: In 264 subjects with an average age of 71.9 (SD: ±4.2), 39% of whom were men, 36.4% had good adherence (score ≥ 9 in MEDAS-14), with no differences by gender or age. The prevalence of MetS was 40.2%, with 47.6% in men and 35.4% in women (p < 0.05). The prevalence of MetS was 2.4 times more frequent among individuals who consumed less than two servings (200 g) of vegetables daily compared with those who consumed two or more servings of vegetables daily (OR: 2.368, 95%CI: 1.141–4.916, p = 0.021). Low adherence to the MedDiet (MEDAS-14 score ≤ 8) was associated with an 82% higher prevalence of MetS (OR: 1.817, 95%CI: 1.072–3.081, p = 0.027). Conclusion: An inverse relationship was established between adherence to the MedDiet and the prevalence of MetS metadata Cubas-Basterrechea, Gloria and Elío Pascual, Iñaki and Alonso, Guzmán and Otero, Luis and Gutiérrez-Bardeci, Luis and Puente, Jesús and Muñoz-Cacho, Pedro mail UNSPECIFIED, inaki.elio@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2022) Adherence to the Mediterranean Diet Is Inversely Associated with the Prevalence of Metabolic Syndrome in Older People from the North of Spain. Nutrients, 14 (21). p. 4536. ISSN 2072-6643

Article Subjects > Biomedicine
Subjects > Engineering
Europe University of Atlantic > Research > Scientific Production
Fundación Universitaria Internacional de Colombia > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Universidad Internacional do Cuanza > Research > Scientific Production
Abierto Inglés Artificial intelligence has been widely used in the field of dentistry in recent years. The present study highlights current advances and limitations in integrating artificial intelligence, machine learning, and deep learning in subfields of dentistry including periodontology, endodontics, orthodontics, restorative dentistry, and oral pathology. This article aims to provide a systematic review of current clinical applications of artificial intelligence within different fields of dentistry. The preferred reporting items for systematic reviews (PRISMA) statement was used as a formal guideline for data collection. Data was obtained from research studies for 2009–2022. The analysis included a total of 55 papers from Google Scholar, IEEE, PubMed, and Scopus databases. Results show that artificial intelligence has the potential to improve dental care, disease diagnosis and prognosis, treatment planning, and risk assessment. Finally, this study highlights the limitations of the analyzed studies and provides future directions to improve dental care metadata Fatima, Anum and Shafi, Imran and Afzal, Hammad and Díez, Isabel De La Torre and Lourdes, Del Rio-Solá M. and Breñosa, Jose and Martínez Espinosa, Julio César and Ashraf, Imran mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, josemanuel.brenosa@uneatlantico.es, ulio.martinez@unini.edu.mx, UNSPECIFIED (2022) Advancements in Dentistry with Artificial Intelligence: Current Clinical Applications and Future Perspectives. Healthcare, 10 (11). p. 2188. ISSN 2227-9032

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Abierto Inglés Excess alcohol consumption is known to be detrimental to human health. However, the role of light-to-moderate alcohol intake is under investigation for potential certain health benefits—mostly related to the cardiovascular system. Nevertheless, there is no univocal agreement on this matter, and research is still ongoing to clarify whether there might be other potential outcomes affected by alcohol intake. In this regard, there is evidence that excess alcohol intake may negatively influence the risk of osteoporotic fractures. However, there is no comprehensive evidence of literature assessing the role of alcohol consumption in bone mineral density (BMD) and the risk of osteoporotic fractures. Thus, the aim of this study was to quantitatively assess the dose–response relationship between alcohol intake and BMD and risk of osteoporotic fractures. The Embase and MEDLINE electronic databases were searched from their inception to December 2021 for articles providing a quantifiable measurement of alcohol consumption for at least three categories and (1) a measurement of BMD (and dispersion as continuous variables) in some area of the body or (2) risk of osteoporotic fracture provided as relative risk (RR) or hazard ratio (HR), with a 95% confidence interval (CI) as the measure of the association of each category with alcohol intake. A total of 11 studies including 46,916 individuals with BMD assessment and 8 studies including 240,871 individuals with risk of fracture analysis were included. Compared to non-drinkers, consumption of up to two standard drinks of alcohol per day was correlated with higher lumbar and femur neck BMD values, while up to one standard drink of alcohol was correlated with higher hip BMD compared to no alcohol consumption. Higher risk of hip fractures was found starting from three standard drinks of alcohol per day (RR = 1.33, 95% CI: 1.04; 1.69 for three alcoholic drinks/d, and RR = 1.59, 95% CI: 1.23; 2.05 for four alcoholic drinks/d) compared to no alcohol consumption, with no evidence of heterogeneity. Concerning the risk of any osteoporotic fractures, the risk steadily increased with higher intake of alcohol, although never reaching statistical significance. In conclusion, there is consistent evidence that increased alcohol consumption is associated with higher risk of osteoporotic hip fracture; however, the role of alcohol at lower doses is uncertain, as BMD was even higher in light drinkers compared to abstainers. metadata Godos, Justyna and Giampieri, Francesca and Chisari, Emanuele and Micek, Agnieszka and Paladino, Nadia and Forbes-Hernández, Tamara Y. and Quiles, José L. and Battino, Maurizio and La Vignera, Sandro and Musumeci, Giuseppe and Grosso, Giuseppe mail UNSPECIFIED, francesca.giampieri@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, jose.quiles@uneatlantico.es, maurizio.battino@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2022) Alcohol Consumption, Bone Mineral Density, and Risk of Osteoporotic Fractures: A Dose–Response Meta-Analysis. International Journal of Environmental Research and Public Health, 19 (3). p. 1515. ISSN 1660-4601

Article Subjects > Biomedicine
Subjects > Nutrition
Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Abierto Inglés Alzheimer's is a chronic degenerative disease of the central nervous system considered the leading cause of dementia in the world. It is characterized by two etiopathological events related to oxidative stress: the aggregation of β-amyloid peptide and the formation of neurofibrillary tangles of hyperphosphorylated Tau protein in the brain. The incidence of this disease increases with age and has been associated with inadequate lifestyles. Some natural compounds have been shown to improve the hallmarks of the disease. However, despite its potential, there is no scientific evidence about Manuka honey (MH) in this regard. In the present work we evaluated the effect of MH on the toxicity induced by Aβ aggregation and Tau in a Caenorhabditis elegans model. Our results demonstrated that MH was able to improve indicators of oxidative stress and delayed Aβ-induced paralysis in the AD model CL4176 through HSP-16.2 and SKN-1/NRF2 pathways. Nevertheless, its sugar content impaired the indicators of locomotion (an indicator of tau neurotoxicity) in both the transgenic strain BR5706 and in the wild-type N2 worms. metadata Navarro-Hortal, María D. and Romero-Márquez, Jose M. and Muñoz-Ollero, Pedro and Jiménez-Trigo, Victoria and Esteban-Muñoz, Adelaida and Tutusaus, Kilian and Giampieri, Francesca and Battino, Maurizio and Sánchez-González, Cristina and Rivas-García, Lorenzo and Llopis, Juan and Forbes-Hernández, Tamara Y. and Quiles, José L. mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, kilian.tutusaus@uneatlantico.es, francesca.giampieri@uneatlantico.es, maurizio.battino@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, jose.quiles@uneatlantico.es (2022) Amyloid β-but not Tau-induced neurotoxicity is suppressed by Manuka honey via HSP-16.2 and SKN-1/Nrf2 pathways in an in vivo model of Alzheimer's disease. Food & Function. ISSN 2042-6496

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Abierto Inglés In this work, we performed a methodological comparative analysis to synthesize polyethyleneimine (PEI) nanoparticles using (i) conventional nanoprecipitation (NP), (ii) electrospraying (ES), and (iii) coaxial electrospraying (CA). The nanoparticles transported antisense oligonucleotides (ASOs), either encapsulated (CA nanocomplexes) or electrostatically bound externally (NP and ES nanocomplexes). After synthesis, the PEI/ASO nanoconjugates were functionalized with a muscle-specific RNA aptamer. Using this combinatorial formulation methodology, we obtained nanocomplexes that were further used as nanocarriers for the delivery of RNA therapeutics (ASO), specifically into muscle cells. In particular, we performed a detailed confocal microscopy-based comparative study to analyze the overall transfection efficiency, the cell-to-cell homogeneity, and the mean fluorescence intensity per cell of micron-sized domains enriched with the nanocomplexes. Furthermore, using high-magnification electron microscopy, we were able to describe, in detail, the ultrastructural basis of the cellular uptake and intracellular trafficking of nanocomplexes by the clathrin-independent endocytic pathway. Our results are a clear demonstration that coaxial electrospraying is a promising methodology for the synthesis of therapeutic nanoparticle-based carriers. Some of the principal features that the nanoparticles synthesized by coaxial electrospraying exhibit are efficient RNA-based drug encapsulation, increased nanoparticle surface availability for aptamer functionalization, a high transfection efficiency, and hyperactivation of the endocytosis and early/late endosome route as the main intracellular uptake mechanism metadata de la Hoz, Raquel and Diban, Nazely and Berciano, María T. and San Emeterio, Carlos and Urtiaga, Ane and Lafarga, Miguel and Rodríguez-Rey, José C. and Tapia Martínez, Olga mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, carlos.sanemeterio@alumnos.uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, olga.tapia@uneatlantico.es (2022) Coaxial Synthesis of PEI-Based Nanocarriers of Encapsulated RNA-Therapeutics to Specifically Target Muscle Cells. Biomolecules, 12 (8). p. 1012. ISSN 2218-273X

Article Subjects > Biomedicine
Subjects > Engineering
Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Universidad Internacional do Cuanza > Research > Scientific Production
Abierto Inglés Asthma is a deadly disease that affects the lungs and air supply of the human body. Coronavirus and its variants also affect the airways of the lungs. Asthma patients approach hospitals mostly in a critical condition and require emergency treatment, which creates a burden on health institutions during pandemics. The similar symptoms of asthma and coronavirus create confusion for health workers during patient handling and treatment of disease. The unavailability of patient history to physicians causes complications in proper diagnostics and treatments. Many asthma patient deaths have been reported especially during pandemics, which necessitates an efficient framework for asthma patients. In this article, we have proposed a blockchain consortium healthcare framework for asthma patients. The proposed framework helps in managing asthma healthcare units, coronavirus patient records and vaccination centers, insurance companies, and government agencies, which are connected through the secure blockchain network. The proposed framework increases data security and scalability as it stores encrypted patient data on the Interplanetary File System (IPFS) and keeps data hash values on the blockchain. The patient data are traceable and accessible to physicians and stakeholders, which helps in accurate diagnostics, timely treatment, and the management of patients. The smart contract ensures the execution of all business rules. The patient profile generation mechanism is also discussed. The experiment results revealed that the proposed framework has better transaction throughput, query delay, and security than existing solutions metadata Farooq, Muhammad Shoaib and Suhail, Maryam and Qureshi, Junaid Nasir and Rustam, Furqan and de la Torre Díez, Isabel and Vidal Mazón, Juan Luis and Rodríguez Velasco, Carmen Lilí and Ashraf, Imran mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, juanluis.vidal@uneatlantico.es, carmen.rodriguez@uneatlantico.es, UNSPECIFIED (2022) Consortium Framework Using Blockchain for Asthma Healthcare in Pandemics. Sensors, 22 (21). p. 8582. ISSN 1424-8220

Article Subjects > Biomedicine
Subjects > Engineering
Europe University of Atlantic > Research > Scientific Production
Fundación Universitaria Internacional de Colombia > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Universidad Internacional do Cuanza > Research > Scientific Production
Abierto Español Patient care and convenience remain the concern of medical professionals and caregivers alike. An unconscious patient confined to a bed may develop fluid accumulation and pressure sores due to inactivity and deficiency of oxygen flow. Moreover, weight monitoring is crucial for an effective treatment plan, which is difficult to measure for bedridden patients. This paper presents the design and development of a smart and cost-effective independent system for lateral rotation, movement, weight measurement, and transporting immobile patients. Optimal dimensions and practical design specifications are determined by a survey across various hospitals. Subsequently, the proposed hoist-based weighing and turning mechanism is CAD-modeled and simulated. Later, the structural analysis is carried out to select suitable metallurgy for various sub-assemblies to ensure design reliability. After fabrication, optimization, integration, and testing procedures, the base frame is designed to mount a hydraulic motor for the actuator, a DC power source for self-sustenance, and lockable wheels for portability. The installation of a weighing scale and a hydraulic actuator is ensured to lift the patient for weight measuring up to 600 pounds or lateral turning of 80 degrees both ways. The developed system offers simple operating characteristics, allows for keeping patient weight records, and assists nurses in changing patients’ lateral positions both ways, comfortably massage patients’ backs, and transport them from one bed to another. Additionally, being lightweight offers reduced contact with the patient to increase the healthcare staff’s safety in pandemics; it is also height adjustable and portable, allowing for use with multiple-sized beds and easy transportation across the medical facility. The feedback from paramedics is encouraging regarding reducing labor-intensive nursing tasks, alleviating the discomfort of long-term bed-ridden patients, and allowing medical practitioners to suggest better treatment plans metadata Shafi, Imran and Farooq, Muhammad Siddique and De La Torre Díez, Isabel and Breñosa, Jose and Martínez Espinosa, Julio César and Ashraf, Imran mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, josemanuel.brenosa@uneatlantico.es, ulio.martinez@unini.edu.mx, UNSPECIFIED (2022) Design and Development of Smart Weight Measurement, Lateral Turning and Transfer Bedding for Unconscious Patients in Pandemics. Healthcare, 10 (11). p. 2174. ISSN 2227-9032

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Abierto Inglés Background: Aging society faces significant health challenges, among which cognitive-related disorders are emerging. Diet quality has been recognized among the major contributors to the rising prevalence of cognitive disorders, with increasing evidence of the putative role of plant-based foods and their bioactive components, including polyphenols. Dietary polyphenols, including phytoestrogens, have been hypothesized to exert beneficial effects toward brain health through various molecular mechanisms. However, the evidence on the association between dietary phytoestrogen intake and cognitive function is limited. The aim of this study was to investigate the association between phytoestrogen intake and cognitive status in a cohort of older adults living in Sicily, Southern Italy. Methods: Dietary information from 883 individuals aged 50 years or older was collected through a validated food frequency questionnaire. Cognitive status was assessed through the Short Portable Mental Status Questionnaire. Results: The highest total isoflavone (including daidzein and genistein) intake was inversely associated with cognitive impairment compared to the lowest (odds ratio (OR) = 0.43, 95% confidence interval (CI): 0.20–0.92). Higher intake of total lignans and, consistently, all individual compounds (with the exception of secoisolariciresinol) were inversely associated with cognitive impairment only in the unadjusted model. Conclusions: A higher intake of phytoestrogens, especially isoflavones, was associated with a better cognitive status in a cohort of older Italian individuals living in Sicily. Taking into account the very low intake of isoflavones in Italian diets, it is noteworthy to further investigate selected populations with habitual consumption of such compounds to test whether these results may be generalized to the Italian population. metadata Giampieri, Francesca and Godos, Justyna and Caruso, Giuseppe and Owczarek, Marcin and Jurek, Joanna and Castellano, Sabrina and Ferri, Raffaele and Caraci, Filippo and Grosso, Giuseppe mail francesca.giampieri@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2022) Dietary Phytoestrogen Intake and Cognitive Status in Southern Italian Older Adults. Biomolecules, 12 (6). p. 760. ISSN 2218-273X

Article Subjects > Biomedicine
Subjects > Engineering
Europe University of Atlantic > Research > Scientific Production
Fundación Universitaria Internacional de Colombia > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Universidad Internacional do Cuanza > Research > Scientific Production
Abierto Inglés The diagnosis of early-stage lung cancer is challenging due to its asymptomatic nature, especially given the repeated radiation exposure and high cost of computed tomography(CT). Examining the lung CT images to detect pulmonary nodules, especially the cell lung cancer lesions, is also tedious and prone to errors even by a specialist. This study proposes a cancer diagnostic model based on a deep learning-enabled support vector machine (SVM). The proposed computer-aided design (CAD) model identifies the physiological and pathological changes in the soft tissues of the cross-section in lung cancer lesions. The model is first trained to recognize lung cancer by measuring and comparing the selected profile values in CT images obtained from patients and control patients at their diagnosis. Then, the model is tested and validated using the CT scans of both patients and control patients that are not shown in the training phase. The study investigates 888 annotated CT scans from the publicly available LIDC/IDRI database. The proposed deep learning-assisted SVM-based model yields 94% accuracy for pulmonary nodule detection representing early-stage lung cancer. It is found superior to other existing methods including complex deep learning, simple machine learning, and the hybrid techniques used on lung CT images for nodule detection. Experimental results demonstrate that the proposed approach can greatly assist radiologists in detecting early lung cancer and facilitating the timely management of patients. metadata Shafi, Imran and Din, Sadia and Khan, Asim and Díez, Isabel De La Torre and Pali-Casanova, Ramón and Tutusaus, Kilian and Ashraf, Imran mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, ramon.pali@unini.edu.mx, kilian.tutusaus@uneatlantico.es, UNSPECIFIED (2022) An Effective Method for Lung Cancer Diagnosis from CT Scan Using Deep Learning-Based Support Vector Network. Cancers, 14 (21). p. 5457. ISSN 2072-6694

Other Subjects > Biomedicine Europe University of Atlantic > Research > Projects I+D+I Cerrado Español Se llevará a cabo un ensayo para estudiar las propiedades antibacterianas de un recubrimiento de material de un tejido en el que se probarán varios compuestos con diferente formulación a fin de determinar la actividad antibacteriana, antimicrobiana o incluso antiadherente de bacterias Gram positivas y Gram negativas de interés en el ámbito hospitalario o en el ámbito de seguridad alimentaria (restauración colectiva). Específicamente los patógenos a probar serán: Staphylococcus aureus subsp. aureus, Listeria monocytogenes, Escherichia coli y Salmonella enterica subsp. entérica. Por otro lado, se estudiarán también las propiedades de dos bacterias marinas (Shewanella báltica y Vibrio vulnificus) que habitualmente forman biofouling en objetos sumergidos en agua salada. En este ensayo se aplicarán estudios de adherencia y proliferación de bacterias in vitro usando placas de 24 pocillos en las que se depositarán fragmentos de 1 cm de diámetro dentro de cada uno de ellos a fin de estudiar las propiedades de las bacterias a ensayar utilizando los medios de cultivo adecuados para cada una de las especies. El ensayo en placas se realizará para cada material o combinación de material/recubrimiento, al menos, por triplicado tomando muestras a diferentes tiempos durante 48 - 96 horas para estudiar el efecto de estas matrices frente a los patógenos previamente mencionados. El método para estudiar la adherencia, la proliferación, la formación de biofilm o la supervivencia de las bacterias sobre las superficies será el recuento de unidades de colonias en placa mediante un método turbidimétrico por densidad óptica a 600 nm realizando las lecturas con un equipo Varioscan Lux. De manera opcional, se podría aplicar la microscopía confocal al estudio de la superficie de los fragmentos de tejido para analizar en detalle el factor de adherencia. metadata CITICAN-Universidad Europea del Atlántico, mail UNSPECIFIED (2022) Ensayo para estudiar las propiedades antibacterianas en tejido textil recubierto. Repositorio de la Universidad. (Unpublished)

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Abierto Inglés The connective tissue or fascia plays key roles in maintaining bodily function and health. The fascia is made up of solid and fluid portions, which interpenetrate and interact with each other, forming a polymorphic three-dimensional network. In the vast panorama of literature there is no univocal thought on the nomenclature and terminology that best represents the concept of fascia. The Foundation of Osteopathic Research and Clinical Endorsement (FORCE) organization brings together various scientific figures in a multidisciplinary perspective. FORCE tries to find a common nomenclature that can be shared, starting from the scientific notions currently available. Knowledge of the fascial continuum should always be at the service of the clinician and never become an exclusive for the presence of copyright, or commodified for the gain of a few. FORCE is a non-profit organization serving all professionals who deal with patient health. The article reviews the concepts of fascia, including some science subjects rarely considered, to gain an understanding of the broader fascial topic, and proposing new concepts, such as the holographic fascia. metadata Bordoni, Bruno and Escher, Allan R and Tobbi, Filippo and Pianese, Luigi and Ciardo, Antonio and Yamahata, Jay and Hernandez, Saul and Sanchez, Oscar mail UNSPECIFIED (2022) Fascial Nomenclature: Update 2022. Cureus, 14 (6). ISSN 2168-8184

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Abierto Inglés When you are part of a community, especially a scientific one, you are required to contribute significantly to its welfare, because the community as a whole represents each individual within it and, in turn, determines the wellbeing of the participants themselves. metadata Battino, Maurizio mail maurizio.battino@uneatlantico.es (2022) Feature Paper Special Issue for Editorial Board Members (EBMs) of Diseases. Diseases, 10 (2). p. 18. ISSN 2079-9721

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Abierto Inglés Antimicrobial-resistant pathogenic bacteria are an increasing problem in public health, especially in the healthcare environment, where nosocomial infection microorganisms find their niche. Among these bacteria, the genus Acinetobacter which belongs to the ESKAPE pathogenic group harbors different multi-drug resistant (MDR) species that cause human nosocomial infections. Although A. baumannii has always attracted more interest, the close-related species A. pittii is the object of more study due to the increase in its isolation and MDR strains. In this work, we present the genomic analysis of five clinically isolated A. pittii strains from a Spanish hospital, with special attention to their genetic resistance determinants and plasmid structures. All the strains harbored different genes related to β-lactam resistance, as well as different MDR efflux pumps. We also found and described, for the first time in this species, point mutations that seem linked with colistin resistance, which highlights the relevance of this comparative analysis among the pathogenic species isolates. metadata Chapartegui-González, Itziar and Lázaro-Díez, María and Ramos Vivas, Jose mail UNSPECIFIED, UNSPECIFIED, jose.ramos@uneatlantico.es (2022) Genetic Resistance Determinants in Clinical Acinetobacter pittii Genomes. Antibiotics, 11 (5). p. 676. ISSN 2079-6382

Article Subjects > Biomedicine
Subjects > Nutrition
Europe University of Atlantic > Research > Scientific Production Abierto Inglés The chemical composition and biological capacities of berries depend on environmental parameters, maturity, and location. The Andean blueberry (Vaccinium floribundum Kunth), also known as mortiño, presents a unique combination of several phytochemicals, which play a synergistic role in its characterization as a functional food. We aimed to expose the possible variations that exist in the profile of the phenolic compounds as well as the antioxidant and antimicrobial capacity of the wild Andean blueberry with respect to three ripeness stages and two different altitudes. We found that polyphenols are the predominant compounds in the berry during the early ripeness stage and are the main bioactive compounds that give rise to the antioxidant capacity and inhibition effect on the growth of gram-positive and gram-negative bacteria. Moreover, the accumulation of ascorbic acid, free amino acids, and anthocyanins increases as the ripening process progresses, and they were the main bioactive compounds in the ripe berry. The latter compounds influence the production of the typical bluish or reddish coloration of ripe blueberries. In addition, it was determined that environmental conditions at high altitudes could have a positive influence in all cases. Overall, our data provide evidence regarding the high functional value of the wild Andean blueberry. metadata Guevara-Terán, Mabel and Padilla-Arias, Katherine and Beltrán-Novoa, Andrea and González-Paramás, Ana M. and Giampieri, Francesca and Battino, Maurizio and Vásquez-Castillo, Wilson and Fernandez-Soto, Paulina and Tejera, Eduardo and Alvarez-Suarez, José M. mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, francesca.giampieri@uneatlantico.es, maurizio.battino@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2022) Influence of Altitudes and Development Stages on the Chemical Composition, Antioxidant, and Antimicrobial Capacity of the Wild Andean Blueberry (Vaccinium floribundum Kunth). Molecules, 27 (21). p. 7525. ISSN 1420-3049

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Abierto Inglés Composites of polymer and graphene-based nanomaterials (GBNs) combine easy processing onto porous 3D membrane geometries due to the polymer and cellular differentiation stimuli due to GBNs fillers. Aiming to step forward to the clinical application of polymer/GBNs composites, this study performs a systematic and detailed comparative analysis of the influence of the properties of four different GBNs: (i) graphene oxide obtained from graphite chemically processes (GO); (ii) reduced graphene oxide (rGO); (iii) multilayered graphene produced by mechanical exfoliation method (Gmec); and (iv) low-oxidized graphene via anodic exfoliation (Ganodic); dispersed in polycaprolactone (PCL) porous membranes to induce astrocytic differentiation. PCL/GBN flat membranes were fabricated by phase inversion technique and broadly characterized in morphology and topography, chemical structure, hydrophilicity, protein adsorption, and electrical properties. Cellular assays with rat C6 glioma cells, as model for cell-specific astrocytes, were performed. Remarkably, low GBN loading (0.67 wt%) caused an important difference in the response of the C6 differentiation among PCL/GBN membranes. PCL/rGO and PCL/GO membranes presented the highest biomolecule markers for astrocyte differentiation. Our results pointed to the chemical structural defects in rGO and GO nanomaterials and the protein adsorption mechanisms as the most plausible cause conferring distinctive properties to PCL/GBN membranes for the promotion of astrocytic differentiation. Overall, our systematic comparative study provides generalizable conclusions and new evidences to discern the role of GBNs features for future research on 3D PCL/graphene composite hollow fiber membranes for in vitro neural models. metadata Mantecón-Oria, Marián and Tapia Martínez, Olga and Lafarga, Miguel and Berciano, María T. and Munuera, Jose M. and Villar-Rodil, Silvia and Paredes, Juan I. and Rivero, María J. and Diban, Nazely and Urtiaga, Ane mail UNSPECIFIED, olga.tapia@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2022) Influence of the properties of different graphene-based nanomaterials dispersed in polycaprolactone membranes on astrocytic differentiation. Scientific Reports, 12 (1). ISSN 2045-2322

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Cerrado Inglés Yersiniosis, caused by the fish pathogen Yersinia ruckeri, is a serious bacterial septicaemia affecting mainly salmonids worldwide. The acute infection may result in high mortality without apparent external disease signs, while the chronic one causes moderate to considerable mortality. Survivors of yersiniosis outbreaks become carriers. Y. ruckeri is able to adhere to, and to invade, phagocytic and non-phagocytic fish cells by using unknown molecular mechanisms. The aim of this study was to describe the kinetics of cell invasion by Y. ruckeri serotype O1 biotype 1 in a fish cell line (RTG-2) originating from rainbow trout gonads. The efficiency of invasion by Y. ruckeri was found to be temperature dependent, having a maximum at 20 °C. The bacterium was able to survive up to 96 h postinfection. The incubation of the cells at 4 °C and the pre-incubation of the bacteria with sugars or heat-inactivated antiserum significantly decreased the efficiency of invasion or even completely prevented the invasion of RTG-2 cells. These findings indicate that Y. ruckeri is capable of adhering to, entering and surviving within non-phagocytic cells, and that the intracellular environment may constitute a suitable niche for this pathogen that can favour the spread of infection and/or the maintenance of a carrier state of fish. metadata Padilla, Daniel and Acosta Hernández, Begoña and Ramos Vivas, Jose and Déniz, Soraya and Rosario, Inmaculada and Martín Barrasa, José Luís and Henao, Andrés sánchez and Silva Sergent, Freddy and Ramos Sosa, María josé and García Álvarez, Natalia and Real, Fernando mail UNSPECIFIED, UNSPECIFIED, jose.ramos@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2022) Kinetics of the invasion of a non-phagocytic fish cell line, RTG-2 by Yersinia ruckeri serotype O1 biotype 1. Acta Veterinaria Hungarica. ISSN 0236-6290

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Abierto Inglés Nuclear envelope integrity is essential for compartmentalisation of nucleus and cytoplasm. Importantly, mutations in nuclear envelope-encoding genes are the second-highest cause of familial dilated cardiomyopathy. One such nuclear envelope protein that causes cardiomyopathy in humans and affects mouse heart development is Lem2. However, its role in mechanically active tissue such as heart remains poorly understood. We generated mice in which Lem2 was specifically ablated in cardiomyocytes and carried out detailed physiological, tissue and cellular analyses. Importantly, our data showed that Lem2 was essential for cardiac development, and hearts from Lem2 cKO mice were morphologically and transcriptionally underdeveloped. Lem2 cKO hearts displayed high levels of DNA damage, nuclear rupture, and apoptosis. Crucially, we found that these defects were driven by muscle contraction as they were ameliorated by inhibiting myosin contraction and conversely were exacerbated upon myosin activation. Our data suggest that Lem2 is critical for integrity at the nascent nuclear envelope in fetal hearts, and protects the nucleus from the mechanical forces of muscle contraction. Taken together, these data provide novel insight into mechanisms underlying striated muscle diseases caused by altered nuclear envelope integrity. metadata Ross, Jacob A. and Arcos-Villacis, Nathaly and Battey, Edmund and Boogerd, Cornelis and Marhuenda, Emilie and Hodzic, Didier and Prin, Fabrice and Mohun, Tim and Catibog, Norman and Tapia Martínez, Olga and Gerace, Larry and Iskratsch, Thomas and Shah, Ajay M. and Stroud, Matthew J. mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, olga.tapia@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2022) Lem2 is essential for cardiac development by maintaining nuclear integrity. bioRxiv. (Unpublished)

Article Subjects > Biomedicine
Subjects > Nutrition
Europe University of Atlantic > Research > Scientific Production Cerrado Inglés Ocimum basilicum and Ocimum tenuiflorum are two basil species widely used medicinally as an anti-inflammatory, antimicrobial and cardioprotective agent. This study focuses on the chemical characterization of the majoritarian compounds of both species and their anti-inflammatory potential. Up to 22 compounds such as various types of salvianolic acids, derivatives of rosmaniric acid and flavones were identified in both plants. The identified compounds were very similar between both plants and are consistent with previous finding in other studies in Portugal and Italy. Based on the identified molecules a consensus target prediction was carried out. Among the main predicted target proteins, we found a high representation of the carbonic anhydrase family (CA2, CA7 and CA12) and several key proteins from the arachidonic pathway (LOX5, PLA2, COX1 and COX2). Both pathways are well related to inflammation. The interaction between the compounds and these targets were explored through molecular docking and molecular dynamics simulation. Our results suggest that some molecules present in both plants can induce an anti-inflammatory response through a non-steroidal mechanism of action connected to the carbon dioxide metabolism. metadata Beltrán-Noboa, Andrea and Proaño-Ojeda, John and Guevara, Mabel and Gallo, Blanca and Berrueta, Luis A. and Giampieri, Francesca and Perez-Castillo, Yunierkis and Battino, Maurizio and Álvarez-Suarez, José M. and Tejera, Eduardo mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, francesca.giampieri@uneatlantico.es, UNSPECIFIED, maurizio.battino@uneatlantico.es, UNSPECIFIED, UNSPECIFIED (2022) Metabolomic profile and computational analysis for the identification of the potential anti-inflammatory mechanisms of action of the traditional medicinal plants Ocimum basilicum and Ocimum tenuiflorum. Food and Chemical Toxicology, 164. p. 113039. ISSN 02786915

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Abierto Inglés Hafnia alvei is receiving increasing attention from both a medical and veterinary point of view, but the diversity of molecules it produces has made the interest in this bacterium extend to the field of probiotics, the microbiota, and above all, to its presence and action on consumer foods. The production of Acyl Homoserine Lactones (AHLs), a type of quorum-sensing (QS) signaling molecule, is the most often-studied chemical signaling molecule in Gram-negative bacteria. H. alvei can use this communication mechanism to promote the expression of certain enzymatic activities in fermented foods, where this bacterium is frequently present. H. alvei also produces a series of molecules involved in the modification of the organoleptic properties of different products, especially cheeses, where it shares space with other microorganisms. Although some strains of this species are implicated in infections in humans, many produce antibacterial compounds, such as bacteriocins, that inhibit the growth of true pathogens, so the characterization of these molecules could be very interesting from the point of view of clinical medicine and the food industry. Lastly, in some cases, H. alvei is responsible for the production of biogenic amines or other compounds of special interest in food health. In this article, we will review the most interesting molecules that produce the H. alvei strains and will discuss some of their properties, both from the point of view of their biological activity on other microorganisms and the properties of different food matrices in which this bacterium usually thrives. metadata Ramos Vivas, Jose and Tapia Martínez, Olga and Elexpuru Zabaleta, Maria and Tutusaus, Kilian and Armas Diaz, Yasmany and Battino, Maurizio and Giampieri, Francesca mail jose.ramos@uneatlantico.es, olga.tapia@uneatlantico.es, maria.elexpuru@uneatlantico.es, kilian.tutusaus@uneatlantico.es, UNSPECIFIED, maurizio.battino@uneatlantico.es, francesca.giampieri@uneatlantico.es (2022) The Molecular Weaponry Produced by the Bacterium Hafnia alvei in Foods. Molecules, 27 (17). p. 5585. ISSN 1420-3049

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Abierto Inglés Background Recent advances from studies of graphene and graphene-based derivatives have highlighted the great potential of these nanomaterials as migrastatic agents with the ability to modulate tumor microenvironments. Nevertheless, the administration of graphene nanomaterials in suspensions in vivo is controversial. As an alternative approach, herein, we report the immobilization of high concentrations of graphene nanoplatelets in polyacrylonitrile film substrates (named PAN/G10) and evaluate their potential use as migrastatic agents on cancer cells. Results Breast cancer MCF7 cells cultured on PAN/G10 substrates presented features resembling mesenchymal-to-epithelial transition, e.g., (i) inhibition of migratory activity; (ii) activation of the expression of E-cadherin, cytokeratin 18, ZO-1 and EpCAM, four key molecular markers of epithelial differentiation; (iii) formation of adherens junctions with clustering and adhesion of cancer cells in aggregates or islets, and (iv) reorganization of the actin cytoskeleton resulting in a polygonal cell shape. Remarkably, assessment with Raman spectroscopy revealed that the above-mentioned events were produced when MCF7 cells were preferentially located on top of graphene-rich regions of the PAN/G10 substrates. Conclusions The present data demonstrate the capacity of these composite substrates to induce an epithelial-like differentiation in MCF7 breast cancer cells, resulting in a migrastatic effect without any chemical agent-mediated signaling. Future works will aim to thoroughly evaluate the mechanisms of how PAN/G10 substrates trigger these responses in cancer cells and their potential use as antimetastatics for the treatment of solid cancers. metadata Diban, Nazely and Mantecón-Oria, Marián and Berciano, María T. and Puente-Bedia, Alba and Rivero, María J. and Urtiaga, Ane and Lafarga, Miguel and Tapia Martínez, Olga mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, olga.tapia@uneatlantico.es (2022) Non-homogeneous dispersion of graphene in polyacrylonitrile substrates induces a migrastatic response and epithelial-like differentiation in MCF7 breast cancer cells. Cancer Nanotechnology, 13 (1). ISSN 1868-6958

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Cerrado Inglés Phages are viruses that infect bacteria, relying on their genetic machinery to replicate. To survive the constant attack of phages, bacteria have developed diverse defense strategies to act against them. Nevertheless, phages rapidly co-evolve to overcome these barriers, resulting in a constant, and often surprising, molecular arms race. Thus, some phages have evolved protein inhibitors known as anti-CRISPRs (∼50–150 amino acids), which antagonize the bacterial CRISPR-Cas immune response. To date, around 45 anti-CRISPRs proteins with different mechanisms and structures have been discovered against the CRISPR-Cas type I and type II present in important animal and human pathogens such as Escherichia, Morganella, Klebsiella, Enterococcus, Pseudomonas, Staphylococcus, and Salmonella. Considering the alarming growth of antibiotic resistance, phage therapy, either alone or in combination with antibiotics, appears to be a promising alternative for the treatment of many bacterial infections. In this review, we illustrated the biological and clinical aspects of using phage therapy; furthermore, the CRISPR-Cas mechanism, and the interesting activity of anti-CRISPR proteins as a possible weapon to combat bacteria. metadata Ceballos-Garzon, Andres and Muñoz, Angela B and Plata, Juan D and Sanchez-Quitian, Zilpa A and Ramos Vivas, Jose mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, jose.ramos@uneatlantico.es (2022) Phages, anti-CRISPR proteins, and drug-resistant bacteria: what do we know about this triad? Pathogens and Disease. ISSN 2049-632X

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Abierto Inglés Empathy and lifelong learning are two professional competencies that depend on the four principles of professionalism: humanism, altruism, excellence, and accountability. In occupational health, there is evidence that empathy prevents work distress. However, in the case of lifelong learning, the evidence is still scarce. In addition, recent studies suggest that the development of lifelong learning varies in physicians and nurses and that it is sensitive to the influence of cultural stereotypes associated with professional roles. This study was performed with the purpose of determining the specific role that empathy and lifelong learning play in the reduction in occupational stress. This study included a sample composed by 40 physicians and 40 nurses with high dedication to clinical work in ambulatory consultations from a public healthcare institution in Paraguay. Somatization, exhaustion, and work alienation, described as indicators of occupational stress, were used as dependent variables, whereas empathy, lifelong learning, gender, discipline, professional experience, civil status, and family burden were used as potential predictors. Three multiple regression models explained 32% of the variability of somatization based on a linear relationship with empathy, lifelong learning, and civil status; 73% of the variability of exhaustion based on a linear relationship with empathy, somatization, work alienation, and discipline; and 62% of the variability of work alienation based on a linear relationship with lifelong learning, exhaustion, and discipline. These findings indicate that empathy and lifelong learning play important roles in the prevention of work distress in physicians and nurses. However, this role varies by discipline. metadata Delgado Bolton, Roberto C. and San-Martín, Montserrat and Vivanco, Luis mail UNSPECIFIED, UNSPECIFIED, luis.vivanco@uneatlantico.es (2022) Role of Empathy and Lifelong Learning Abilities in Physicians and Nurses Who Work in Direct Contact with Patients in Adverse Working Conditions. International Journal of Environmental Research and Public Health, 19 (5). p. 3012. ISSN 1660-4601

Article Subjects > Biomedicine
Subjects > Nutrition
Europe University of Atlantic > Research > Scientific Production Abierto Inglés Bioactive compounds from strawberries have been associated with multiple healthy benefits. The present study aimed to assess chemical characterization of a methanolic extract of the Romina strawberry variety in terms of antioxidant capacity, polyphenols profile and chemical elements content. Additionally, potential toxicity, the effect on amyloid-β production and oxidative stress of the extract was in vivo evaluated in the experimental model Caenorhabditis elegans. Results revealed an important content in phenolic compounds (mainly ellagic acid and pelargonidin-3-glucoside) and minerals (K, Mg, P and Ca). The treatment with 100, 500 or 1000 μg/mL of strawberry extract did not show toxicity. On the contrary, the extract was able to delay amyloid β-protein induced paralysis, reduced amyloid-β aggregation and prevented oxidative stress. The potential molecular mechanisms present behind the observed results explored by RNAi technology revealed that DAF-16/FOXO and SKN-1/NRF2 signaling pathways were, at least partially, involved. metadata Navarro-Hortal, María D. and Romero-Márquez, Jose M. and Esteban-Muñoz, Adelaida and Sánchez-González, Cristina and Rivas-García, Lorenzo and Llopis, Juan and Cianciosi, Danila and Giampieri, Francesca and Sumalla Cano, Sandra and Battino, Maurizio and Quiles, José L. mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, sandra.sumalla@uneatlantico.es, UNSPECIFIED, jose.quiles@uneatlantico.es (2022) Strawberry (Fragaria × ananassa cv. Romina) methanolic extract attenuates Alzheimer’s beta amyloid production and oxidative stress by SKN-1/NRF and DAF-16/FOXO mediated mechanisms in C. elegans. Food Chemistry, 372. p. 131272. ISSN 03088146

Article Subjects > Biomedicine
Subjects > Engineering
Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Universidad Internacional do Cuanza > Research > Scientific Production
Abierto Inglés Herbal medicine and nutritional supplements are suggested to treat premenstrual somatic and psycho-behavioural symptoms in clinical guidelines; nonetheless, this is at present based on poor-quality trial evidence. Hence, we aimed to design a systematic review and meta-analysis for their effectiveness in alleviating premenstrual symptoms. The published randomized controlled trials (RCTs) were extracted from Google scholar, PubMed, Scopus and PROSPERO databases. The risk of bias in randomized trials was assessed by Cochrane risk-of-bias tool. The main outcome parameters were analysed separately based on the Premenstrual Symptom Screening Tool and PMTS and DRSP scores. Secondary parameters of somatic, psychological, and behavioural subscale symptoms of PSST were also analysed. Data synthesis was performed assuming a random-effects model, and standardized mean difference (SMDs) was analysed using SPSS version 28.0.0 (IBM, Armonk, NY, USA). A total of 754 articles were screened, and 15 RCTs were included (n = 1211 patients). Primary results for participants randomized to an intervention reported reduced PSST (n = 9), PMTS (n = 2), and DSR (n = 4) scores with (SMD = −1.44; 95% CI: −1.72 to −1.17), (SMD = −1.69; 95% CI: −3.80 to 0.42) and (SMD = 2.86; 95% CI: 1.02 to 4.69) verses comparator with substantial heterogeneity. Physical (SMD = −1.61; 95% CI = −2.56 to −0.66), behavioural (SMD = −0.60; 95% CI = −1.55 to0.35) and mood (SMD = 0.57; 95% CI = −0.96 to 2.11) subscale symptom groupings of PSST displayed similar findings. Fifty-three studies (n = 8) were considered at low risk of bias with high quality. Mild adverse events were reported by four RCTs. Based on the existing evidence, herbal medicine and nutritional supplements may be effective and safe for PMS metadata Sultana, Arshiya and Heyat, Md Belal Bin and Rahman, Khaleequr and Kunnavil, Radhika and Fazmiya, Mohamed Joonus Aynul and Akhtar, Faijan and Sumbul, X. and Vidal Mazón, Juan Luis and Rodríguez Velasco, Carmen Lilí and De La Torre Díez, Isabel mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, juanluis.vidal@uneatlantico.es, carmen.rodriguez@uneatlantico.es, UNSPECIFIED (2022) A Systematic Review and Meta-Analysis of Premenstrual Syndrome with Special Emphasis on Herbal Medicine and Nutritional Supplements. Pharmaceuticals, 15 (11). p. 1371. ISSN 1424-8247

Article Subjects > Biomedicine
Subjects > Engineering
Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Magazines
Ibero-american International University > Research > Scientific Production
Universidad Internacional do Cuanza > Research > Scientific Production
Abierto Inglés White blood cell (WBC) type classification is a task of significant importance for diagnosis using microscopic images of WBC, which develop immunity to fight against infections and foreign substances. WBCs consist of different types, and abnormalities in a type of WBC may potentially represent a disease such as leukemia. Existing studies are limited by low accuracy and overrated performance, often caused by model overfit due to an imbalanced dataset. Additionally, many studies consider a lower number of WBC types, and the accuracy is exaggerated. This study presents a hybrid feature set of selective features and synthetic minority oversampling technique-based resampling to mitigate the influence of the above-mentioned problems. Furthermore, machine learning models are adopted for being less computationally complex, requiring less data for training, and providing robust results. Experiments are performed using both machine- and deep learning models for performance comparison using the original dataset, augmented dataset, and oversampled dataset to analyze the performances of the models. The results suggest that a hybrid feature set of both texture and RGB features from microscopic images, selected using Chi2, produces a high accuracy of 0.97 with random forest. Performance appraisal using k-fold cross-validation and comparison with existing state-of-the-art studies shows that the proposed approach outperforms existing studies regarding the obtained accuracy and computational complexity. metadata Rustam, Furqan and Aslam, Naila and De La Torre Díez, Isabel and Khan, Yaser Daanial and Vidal Mazón, Juan Luis and Rodríguez Velasco, Carmen Lilí and Ashraf, Imran mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, juanluis.vidal@uneatlantico.es, carmen.rodriguez@uneatlantico.es, UNSPECIFIED (2022) White Blood Cell Classification Using Texture and RGB Features of Oversampled Microscopic Images. Healthcare, 10 (11). p. 2230. ISSN 2227-9032

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Abierto Inglés A new palladium coordination compound based on gliclazide with the chemical formula [Pd(glz)2] (where glz = gliclazide) has been synthesized and characterised. The structural characterization reveals that this material consists of mononuclear units formed by a Pd2+ ion coordinated to two molecules of the glz ligand, in which palladium ions exhibit a distorted plane-square coordination sphere. This novel material behaves like a good and selective inhibitor of butyrylcholinesterase, one of the most relevant therapeutic targets against Alzheimer's disease. Analysis of the enzyme kinetics showed a mixed mode of inhibition, the title compound being capable of interacting with both the free enzyme and the enzyme-substrate complex. Finally, the palladium compound shows promising protective activity against Aβ-induced toxicity in the Caenorhabditis elegans model, which has never been reported. metadata García-García, Amalia and Rojas, Sara and Rivas-García, Lorenzo and Navarro-Hortal, María D. and Romero-Márquez, Jose M. and Fernández-Bolaños, José G. and Choquesillo-Lazarte, Duane and Salinas-Castillo, Alfonso and López, Óscar and Quiles, José L. and Rodríguez-Diéguez, Antonio mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, jose.quiles@uneatlantico.es, UNSPECIFIED (2022) A gliclazide complex based on palladium towards Alzheimer's disease: promising protective activity against Aβ-induced toxicity in C. elegans. Chemical Communications, 58 (10). pp. 1514-1517. ISSN 1359-7345

2021

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Abierto Inglés Acinetobacter baumannii is a Gram-negative coccoid rod species, clinically relevant as a human pathogen, included in the ESKAPE group. Carbapenem-resistant A. baumannii (CRAB) are considered by the World Health Organization (WHO) as a critical priority pathogen for the research and development of new antibiotics. Some of the most relevant features of this pathogen are its intrinsic multidrug resistance and its ability to acquire rapid and effective new resistant determinants against last-resort clinical antibiotics, mostly from other ESKAPE species. The presence of plasmids and mobile genetic elements in their genomes contributes to the acquisition of new antimicrobial resistance determinants. However, although A. baumannii has arisen as an important human pathogen, information about these elements is still not well understood. Current genomic analysis availability has increased our ability to understand the microevolution of bacterial pathogens, including point mutations, genetic dissemination, genomic stability, and pan- and core-genome compositions. In this work, we deeply studied the genomes of four clinical strains from our hospital, and the reference strain ATCC®19606TM, which have shown a remarkable ability to survive and maintain their effective capacity when subjected to long-term stress conditions. With that, our aim was presenting a detailed analysis of their genomes, including antibiotic resistance determinants and plasmid composition. metadata Chapartegui-González, Itziar and Lázaro-Díez, María and Redondo-Salvo, Santiago and Navas, Jesús and Ramos-Vivas, José mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, jose.ramos@uneatlantico.es (2021) Antimicrobial Resistance Determinants in Genomes and Plasmids from Acinetobacter baumannii Clinical Isolates. Antibiotics, 10 (7). p. 753. ISSN 2079-6382

Other Subjects > Biomedicine
Subjects > Engineering
Europe University of Atlantic > Research > Projects I+D+I Cerrado Español Background/main objectives: Spinal muscular atrophy (SMA) is a devastating neuromuscular disease characterized by degeneration of spinal cord motoneurons (MNs), muscle weakness, and severe disability. SMA is the most common genetic disease resulting in infantile death. It is caused by reduction of the survival motor neuron (SMN) protein due to the loss or mutations of SMN1 gene. In humans, a second duplicate gene (SMN2) produces little and insufficient functional SMN. Since the discovery of the SMN1 gene, intensive efforts have been aimed to develop therapies to increase SMN protein levels. The SNM2-directed antisense oligonucleotide nusinersen effectively increases SMN in clinical trials, but its effectiveness varies between individuals and is markedly diminished in post-symptomatic treated patients. We aim to examine here the possible beneficial effect of combining nusinersen with drugs directed to the CNS and peripheral tissues that we previously found to have beneficial in vivo and in vitro effects in SMA models. We will investigate how these new approaches change the phenotype and analyze in detail their impact on five hallmarks of the disease, i.e., MN deafferentation, spinal cord neuroinflammation, MN alterations in autophagy, neuromuscular junction neurotransmission deficiency, and skeletal muscle structural alterations. Methodology: In vivo experiments will be conducted in mouse models of SMA, which will be subjected to different single or combined treatments; motor behavior tests and survival analysis will be performed. In vitro studies will be carried out in SMA mouse MNs and in induced pluripotent stem cell (iPSC) lines from control and SMA patients. Immunocytochemistry, western blot, and conventional and pre- and post-embedding immunogold electron microscopy studies will be also performed. Additionally, electrophysiological recordings of endplate potentials will be conducted. Expected results: We expect that the use of SMN-independent strategies in combination with nusinersen will help to ameliorate the neuromuscular and systemic alterations in SMA. metadata Institut de Recerca Biomèdica de Lleida Fundació Dr. Pifarré, IRBLleida and UNEATLANTICO, and Universidad de Sevilla, US mail UNSPECIFIED (2021) Análisis preclínica de nuevos tratamientos combinados para la atrofia muscular espinal: efectos sobre la supervivencia de la motoneurona, la integridad sináptica y la preservación del músculo esquelético. Repositorio de la Universidad. (Unpublished)

Thesis Subjects > Biomedicine Europe University of Atlantic > Teaching > Final Degree Projects Cerrado Español Objetivos: identificar los beneficios de la AF sobre los efectos secundarios y generar una propuesta de intervención. Material y métodos: revisión sistemática de ensayos clínicos controlados aleatorizados en el periodo de Marzo a Abril de 2021. Pacientes con cáncer de mama tratados con quimioterapia sin excluir a los tratados con radioterapia. Intervenciones de actividad física sin incluir estudios piloto ni aquellos que no alcanzaran el nivel de confianza. Resultados: 11 estudios (3 de HIIT, 3 de aeróbico, 2 que combinaban fuerza y aeróbico, 1 de fuerza y 2 de Yoga). De estos artículos solo el 9,09% (1 artículo) no presentó beneficios significativos en las variables analizadas. Mientras que el resto de intervenciones (90,9%) sí que lo hicieron. Conclusiones: Las intervenciones de actividad física producen una disminución de los efectos secundarios de la quimioterapia, así como el trabajo de carácter no supervisado o pertenecer al grupo de control que no realiza entrenamientos, no presentan mejoras. La propuesta de intervención tiene el objetivo de mejorar las aptitudes físicas, la disminución de los efectos secundario. Su duración es de 14 semanas y está programada para ser realizada en cualquier centro con personal cualificado. metadata Sainz Gómez, Mario mail mario.sainz@alumnos.uneatlantico.es (2021) Beneficios del ejercicio físico en personas con cáncer de mama durante la quimioterapia. revisión sistemática y propuesta de intervención. Diploma thesis, Universidad Europea del Atlántico.

Other Subjects > Biomedicine
Subjects > Engineering
Europe University of Atlantic > Research > Projects I+D+I Cerrado Español El reto ambiental que asume el presente proyecto está enmarcado en la gestión de “Residuos del sector primario” dentro del “Plan de Residuos de Cantabria 2017-2023”. El proyecto se enmarca en la aplicación de residuos procedentes de la industria apícola en el ámbito de la biomedicina. La industria productora de cera tiene su origen en el siglo XIX en Alemania. A nivel internacional, la cera de abeja es producida por manufactureros especializados a los cuales los apicultores proporcionan los panales o la cera “cruda”, que después procesan. Para poder ser destinada a fines industriales, cosmética o farmacéutica, tiene que ser, además, purificada por filtración y centrifugada para la eliminación de restos de contaminantes (Stefan Bogdanov, 2009). En España, la producción de cera de abeja se ha mantenido constante a lo largo de los últimos 20 años. Según la última estimación del Ministerio de Agricultura, Pesca y Alimentación, en 2018 se han producido 1,519 toneladas de cera procedente del sector apícola (Programa Nacional de Ayuda a La Apicultura, 2019). En Cantabria, la industria agroalimentaria ha sido y es un pilar fundamental. En concreto la producción y tradición apícola ha pasado de generación en generación siendo signo de identidad en muchas comarcas de la región donde ha impulsado el empleo rural y la conservación del ecosistema. En los últimos años, la cría de abejas en la comunidad ha visto aumentar su trascendencia y difusión gracias a la ganancia de denominaciones de origen protegida (Miel de Liébana) y al incremento de la producción y venta de enjambres causada por la gran demanda a niveles tanto nacional como internacional derivada de lamortalidad de las colmenas (Consejeria de Desarrollo Rural, Ganaderia, Pesca y Biodiversidad, n.d.) La cría de abejas mediante el método tradicional aplicado en Cantabria de los dujos o corchos, permite el crecimiento de los enjambres en panales naturales caracterizados por la obtención de productos más puros y por tanto, con mayor concentración decomponentes bioactivos, debido al ecosistema singular en el que se encuentran, la menor carga parasitaria (por las propias características del panal) y la menor aplicación de tratamientos sanitarios y alimentación artificial (Pliego de Condiciones de La Denominación de Origen Protegida “Miel de Liébana,” n.d.). Precisamente por las características de la apicultura tradicional, creemos que el aprovechamiento o reciclado de los residuos derivados de la producción de la cera de abeja procedente de la comarca de Liébana tendría una potencial aplicación en la investigación biomédica, concretamente en la prevención y/o tratamiento del proceso inflamatorio. Esto promovería el reciclado de los residuos originados en el procesado de la cera de abeja creada mediante el método apícola tradicional, estimulando la economía circular. Además, la utilización de estas matrices podría poner a Cantabria como ejemplo a seguir en la estimulación de la cría de abeja de manera tradicional que, a su vez, favorece la protección de la biodiversidad del ecosistema. metadata CITICAN-Universidad Europea del Atlántico, mail UNSPECIFIED (2021) CAROZO: componentes bioactivos en residuos de producción apícola. Repositorio de la Universidad. (Unpublished)

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Abierto Inglés Biological tissue discrimination is relevant in guided surgery. Nerve identification is critical to avoid potentially severe collateral damage. Fluorescence imaging by oxazine 4-perchlorate (O4P) has been recently proposed. In this work, the cytotoxicity of O4P on U87 human-derived glioma cells has been investigated as a function of concentration and operating room irradiation modes. A custom-built optical irradiation device was employed for controlled optical dosimetry. DNA damage and O4P intracellular localization was also investigated by immunofluorescence and confocal microscopy. The results show that concentration below 100 µM can be considered safe. These results contribute to the assessment of the feasibility of O4P as a nerve biomarker. metadata Pampín-Suárez, Sandra and Arce-Diego, José Luis and Tapia Martínez, Olga and Pérez-Campo, Flor María and Rodríguez-Rey, José Carlos and Fanjul-Vélez, Félix mail UNSPECIFIED, UNSPECIFIED, olga.tapia@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2021) Cytotoxicity analysis of oxazine 4-perchlorate fluorescence nerve potential clinical biomarker for guided surgery. Biomedical Optics Express, 13 (1). p. 197. ISSN 2156-7085

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Abierto Inglés Diabetes and periodontitis are two of the most prevalent diseases worldwide that negatively impact the quality of life of the individual suffering from them. They are part of the chronic inflammatory disease group or, as recently mentioned, non-communicable diseases, with inflammation being the meeting point among them. Inflammation hitherto includes vascular and tissue changes, but new technologies provide data at the intracellular level that could explain how the cells respond to the aggression more clearly. This review aims to emphasize the molecular pathophysiological mechanisms in patients with type 2 diabetes mellitus and periodontitis, which are marked by different impaired central regulators including mitochondrial dysfunction, impaired immune system and autophagy pathways, oxidative stress, and the crosstalk between adenosine monophosphate-activated protein kinase (AMPK) and the renin-angiotensin system (RAS). All of them are the shared background behind both diseases that could explain its relationship. These should be taken in consideration if we would like to improve the treatment outcomes. Currently, the main treatment strategies in diabetes try to reduce glycemia index as the most important aspect, and in periodontitis try to reduce the presence of oral bacteria. We propose to add to the therapeutic guidelines the handling of all the intracellular disorders to try to obtain better treatment success. metadata Portes, Juliana and Bullón, Beatriz and Quiles, José L. and Battino, Maurizio and Bullón, Pedro mail UNSPECIFIED, UNSPECIFIED, jose.quiles@uneatlantico.es, maurizio.battino@uneatlantico.es, UNSPECIFIED (2021) Diabetes Mellitus and Periodontitis Share Intracellular Disorders as the Main Meeting Point. Cells, 10 (9). p. 2411. ISSN 2073-4409

Thesis Subjects > Biomedicine
Subjects > Nutrition
Europe University of Atlantic > Teaching > Final Degree Projects Cerrado Español Estado de la cuestión: El trastorno del espectro autista (TEA) es un trastorno neurológico que afecta al comportamiento y la comunicación y que tiene múltiples etiologías. Actualmente no tiene cura, y por ello se están estudiando diferentes tratamientos sintomáticos, entre los cuáles se encuentra la dieta libre de gluten y caseína. Objetivo: Relacionar la dieta libre de gluten y caseína (GFCF) con la mejora de los síntomas en pacientes con trastorno del espectro autista (TEA).Material y métodos: Se realizó una revisión bibliográfica, y se seleccionaron y consultaron varios artículos científicos. Un total de 12 estudios publicados en los últimos 5 años fueron analizados en profundidad. Resultados y discusión: Existe gran controversia con la GFCF y si esta mejora los síntomas del TEA. En ocasiones, esta dieta ha mejorado tanto síntomas neurológicos como algunos gastrointestinales. Mientras que, en otros casos, no se ha observado mejora o incluso se han producido déficits en algunos nutrientes. Conclusiones: La GFCF no es un tratamiento nutricional recomendado en el TEA. Se necesitan más estudios a largo plazo que estudien el efecto de esta, así como sus posibles efectos secundarios. metadata Malo del Pozo, Leire mail leire.malo@alumnos.uneatlantico.es (2021) Dieta libre de gluten y caseína como tratamiento nutricional del trastorno del espectro autista (TEA). Diploma thesis, Universidad Europea del Atlántico.

Other Subjects > Biomedicine
Subjects > Engineering
Europe University of Atlantic > Research > Projects I+D+I Cerrado Español UNSPECIFIED metadata UNEATLANTICO, mail UNSPECIFIED (2021) Diseño de nanopartículas funcionalizadas con oligonucleótidos antisentido y Marizomib para la terapia génica de la miopatía en la atrofia muscular espinal. Repositorio de la Universidad. (Unpublished)

Thesis Subjects > Biomedicine
Subjects > Physical Education and Sport
Europe University of Atlantic > Teaching > Final Degree Projects Cerrado Español La enfermedad del Parkinson (EP) es un trastorno neurodegenerativo crónico que afecta al sistema nervioso central, lo que conduce a la aparición de síntomas motores y no motores. Se produce un deterioro del movimiento debido a que las extremidades se vuelven rígidas y, además, hay problemas en la coordinación y el equilibrio, lo que aumenta el riesgo de caídas. Todo esto perjudica el día a día de los pacientes. Los objetivos principales de esta revisión sistemática son analizar y evaluar si el ejercicio terapéutico es beneficioso y seguro para tratar la EP, así como comparar los diferentes programas para definir cuáles son los métodos de entrenamiento más eficaces para esta población. Los estudios que se revisaron proceden de las bases de datos PubMed y Google Scholar. Se seleccionaron artículos redactados en español e inglés, publicados en los últimos 10 años y correspondientes a ensayos clínicos aleatorizados. Los estudios mostraron que el ejercicio físico es una alternativa beneficiosa como tratamiento coadyuvante de la EP. Las mejoras más significativas se consiguieron en el equilibrio, la marcha y la funcionalidad de los pacientes. Gracias a estas mejoras, se redujo la gravedad de los síntomas, dando lugar a una mayor calidad de vida. En conclusión, el ejercicio terapéutico realizado de forma supervisada es un método eficaz y seguro para conseguir efectos positivos en la vida diaria de esta población. Con el trabajo combinado de cada una de las capacidades se obtienen beneficios en diversos síntomas de la enfermedad, siendo los ejercicios de equilibrio los que mayor efectividad tienen sobre la sintomatología motora y se mejora de forma significativa la calidad de vida de los pacientes. metadata Gutiérrez Gutiérrez, Sandra mail sandra.gutierrez@alumnos.uneatlantico.es (2021) Efectos de un programa de ejercicio físico en pacientes con parkinson en fase temprana. Revisión sistemática. Diploma thesis, Universidad Europea del Atlántico.

Thesis Subjects > Biomedicine Europe University of Atlantic > Teaching > Final Degree Projects Cerrado Español La bioimpedancia eléctrica es un método para la medida de la composición corporal basado en la capacidad del cuerpo humano para transmitir la corriente eléctrica, siendo por su bajo coste, su comodidad de uso y por ser poco invasivo el más extendido en la práctica clínica. Esta permite medir varios factores de interés en el ámbito clínico entre los que se encuentran la impedancia, la resistencia, reactancia, la capacitancia y por último el ángulo de fase. El principal objetivo del presente estudio fue estudiar la factibilidad del ángulo de fase para determinar el pronóstico de pacientes con diferentes tipos de cáncer. Para ello se incluyeron trabajos en español e inglés, posteriores al año 2004 y obtenidos de las bases de datos Pubmed y National Center for Biotechnology Information (NCBI). Finalmente, se consideraron elegibles para esta revisión 12 estudios que informaban sobre 2505 pacientes. Los trabajos seleccionados se llevaron a cabo en Estados Unidos, México y Corea del Sur. Siete estudios incluyeron una población con tipos específicos de cáncer, como el cáncer de mama, el cáncer de páncreas, el cáncer colorrectal, el cáncer de pulmón y el cáncer de cabeza y cuello. Para los estudios que incluyeron diferentes tipos de cáncer, el cáncer del tracto digestivo fue el tipo más común. Los estudios seleccionados para la revisión obtuvieron diferentes ángulos de fase dependiendo del tipo de cáncer y demostraron que, a mayor ángulo de fase, el pronóstico de vida era mayor. El ángulo de fase, derivado de la bioimpedancia, es un importante factor objetivo de predicción de la supervivencia para los pacientes con diferentes tipos de patologías, pero se necesitan estudios similares de otros tipos de cáncer con tamaños de muestra suficientemente grandes para seguir validando la importancia del pronóstico del ángulo de fase en los entornos clínicos. metadata Madinabeitia Murillo, Jon mail jon.madinabeitia@alumnos.uneatlantico.es (2021) ¿Es el ángulo de fase una herramienta de pronóstico válida para la supervivencia de los pacientes con cáncer ? Diploma thesis, Universidad Europea del Atlántico.

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Abierto Inglés COVID-19 declared as a pandemic that has a faster rate of infection and has impacted the lives and the country’s economy due to forced lockdowns. Its detection using RT-PCR is required long time and due to which its infection has grown exponentially. This creates havoc for the shortage of testing kits in many countries. This work has proposed a new image processing-based technique for the health care systems named “C19D-Net”, to detect “COVID-19” infection from “Chest X-Ray” (XR) images, which can help radiologists to improve their accuracy of detection COVID-19. The proposed system extracts deep learning (DL) features by applying the InceptionV4 architecture and Multiclass SVM classifier to classify and detect COVID-19 infection into four different classes. The dataset of 1900 Chest XR images has been collected from two publicly accessible databases. Images are pre-processed with proper scaling and regular feeding to the proposed model for accuracy attainments. Extensive tests are conducted with the proposed model (“C19D-Net”) and it has succeeded to achieve the highest COVID-19 detection accuracy as 96.24% for 4-classes, 95.51% for three-classes, and 98.1% for two-classes. The proposed method has outperformed well in expressions of “precision”, “accuracy”, “F1-score” and “recall” in comparison with most of the recent previously published methods. As a result, for the present situation of COVID-19, the proposed “C19D-Net” can be employed in places where test kits are in short supply, to help the radiologists to improve their accuracy of detection of COVID-19 patients through XR-Images. metadata Kaur, Prabhjot and Harnal, Shilpi and Tiwari, Rajeev and Alharithi, Fahd S. and Almulihi, Ahmed H. and Delgado Noya, Irene and Goyal, Nitin mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, irene.delgado@uneatlantico.es, UNSPECIFIED (2021) A Hybrid Convolutional Neural Network Model for Diagnosis of COVID-19 Using Chest X-ray Images. International Journal of Environmental Research and Public Health, 18 (22). p. 12191. ISSN 1660-4601

Article Subjects > Biomedicine
Subjects > Nutrition
Europe University of Atlantic > Research > Scientific Production Cerrado Inglés Inflammation is a key mechanism of the immune system that can be elicited by several factors, among them several chemical, physical and biological agents. Once stimulated, the inflammatory response activates a series of signaling pathways and a number of immune cells which promote, in a very coordinated manner, the neutralization of the infectious agent. However, if uncontrolled, the inflammatory status may become chronic leading, potentially, to tissue damage and disease onset. Several risk factors are associated with the development of chronic inflammation and, among these factors, diet plays an essential role. In this chapter the effects of some dietary bioactive compounds, including micronutrients, omega-3 fatty acids, nucleotides and polyphenols, on the immunoinflammatory responses in different cellular, animal and human studies have been summarized. metadata Giampieri, Francesca and Cianciosi, Danila and Ansary, Johura and Elexpuru Zabaleta, Maria and Forbes-Hernandez, Tamara Y. and Battino, Maurizio mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, maria.elexpuru@uneatlantico.es, UNSPECIFIED, UNSPECIFIED (2021) Immunoinflammatory effects of dietary bioactive compounds. Advances in Food and Nutrition Research, 95. pp. 295-336. ISSN 10434526

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Abierto Inglés Inflammation is a hallmark of aging and accelerated aging syndromes such as Hutchinson–Gilford progeria syndrome (HGPS). In this study, we present evidence of increased expression of the components of the NLRP3 inflammasome pathway in HGPS skin fibroblasts, an outcome that was associated with morphological changes of the nuclei of the cells. Lymphoblasts from HGPS patients also showed increased basal levels of NLRP3 and caspase 1. Consistent with these results, the expression of caspase 1 and Nlrp3, but not of the other inflammasome receptors was higher in the heart and liver of Zmpste24−/− mice, which phenocopy the human disease. These data were further corroborated in LmnaG609G/G609G mice, another HGPS animal model. We also showed that pharmacological inhibition of the NLRP3 inflammasome by its selective inhibitor, MCC950, improved cellular phenotype, significantly extended the lifespan of progeroid animals, and reduced inflammasome-dependent inflammation. These findings suggest that inhibition of the NLRP3 inflammasome is a potential therapeutic approach for the treatment of HGPS. metadata González‐Dominguez, Alvaro and Montañez, Raúl and Castejón‐Vega, Beatriz and Nuñez‐Vasco, Jéssica and Lendines‐Cordero, Débora and Wang, Chun and Mbalaviele, Gabriel and Navarro-Pando, José Manuel and Alcocer‐Gómez, Elísabet and Cordero, Mario D mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, jose.navarro@uneatlantico.es, UNSPECIFIED, UNSPECIFIED (2021) Inhibition of the NLRP3 inflammasome improves lifespan in animal murine model of Hutchinson–Gilford Progeria. EMBO Molecular Medicine, 13 (10). ISSN 1757-4676

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production UNSPECIFIED UNSPECIFIED Inflammation is a hallmark of aging and is negatively affecting female fertility. In this study, we evaluate the role of the NLRP3 inflammasome in ovarian aging and female fertility. Age-dependent increased expression of NLRP3 in the ovary was observed in WT mice during reproductive aging. High expression of NLRP3, caspase-1, and IL-1β was also observed in granulosa cells from patients with ovarian insufficiency. Ablation of NLRP3 improved the survival and pregnancy rates and increased anti-Müllerian hormone levels and autophagy rates in ovaries. Deficiency of NLRP3 also reduced serum FSH and estradiol levels. Consistent with these results, pharmacological inhibition of NLRP3 using a direct NLRP3 inhibitor, MCC950, improved fertility in female mice to levels comparable to those of Nlrp3−/− mice. These results suggest that the NLRP3 inflammasome is implicated in the age-dependent loss of female fertility and position this inflammasome as a potential new therapeutic target for the treatment of infertility. metadata Navarro-Pando, José Manuel and Alcocer-Gómez, Elísabet and Castejón-Vega, Beatriz and Navarro-Villarán, Elena and Condés-Hervás, Mónica and Mundi-Roldan, María and Muntané, Jordi and Pérez-Pulido, Antonio J. and Bullon, Pedro and Wang, Chun and Hoffman, Hal M. and Sanz, Alberto and Mbalaviele, Gabriel and Ryffel, Bernhard and Cordero, Mario D. mail jose.navarro@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, monica.condes@uneatlantico.es, maria.mundi@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, mario.cordero@uneatlantico.es (2021) Inhibition of the NLRP3 inflammasome prevents ovarian aging. Science Advances, 7 (1). eabc7409. ISSN 2375-2548

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Abierto Inglés nter-professional collaboration, empathy and lifelong learning, components of medical professionalism, have been associated with occupational well-being in physicians. However, it is not clear whether this role persists in adverse working conditions. This study was performed to assess whether this is the case. These three abilities, and the self-perception of somatization, exhaustion and work alienation, were measured in a sample of 60 physicians working in a hospital declared to be in an institutional emergency. A multiple regression model explained 40% of the variability of exhaustion, with a large effect size (Cohen’s-f2 = 0.64), based on a linear relationship with teamwork (p = 0.01), and more dedication to academic (p < 0.001) and management activities (p < 0.003). Neither somatization nor alienation were predicted by empathy or lifelong learning abilities. Somatization, exhaustion, or alienation scores either explained empathy, inter-professional collaboration or lifelong learning scores. These findings indicate that, in adverse working environments, physicians with a greater sense of inter-professional collaboration or performing multi-task activities are more exposed to suffering exhaustion. metadata Viruez-Soto, José and Delgado Bolton, Roberto C. and San-Martín, Montserrat and Vivanco, Luis mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, luis.vivanco@uneatlantico.es (2021) Inter-Professional Collaboration and Occupational Well-Being of Physicians Who Work in Adverse Working Conditions. Healthcare, 9 (9). p. 1210. ISSN 2227-9032

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Abierto Inglés The purpose of this study was to determine the probability that preschool children have severe motor difficulties or are at risk of motor difficulties, according to quarter of birth and gender. Five hundred and eighty-eight preschool-age children were evaluated, of which 318 (54.08%) were boys and 270 (45.92%) were girls, with a mean age of 4.66 years (SD = 0.53). The Movement Assessment Battery for Children-2 (MABC-2) was used to collect the data. The results obtained were the following: Regarding students with severe motor difficulties: 6.7% born in quarter 1 (Q1); 13.3% born in the second quarter (Q2); 20.0% born in the third quarter (Q3); and 60.0% born in the fourth quarter (Q4). The probabilities found (OR) were: Q1 vs. Q2 (OR = 3.15; p < 0.05); Q1 vs. Q3 (OR = 4.68; p < 0.005); Q1 vs. Q4 (OR = 12.40; p < 0.001); Q2 vs. Q4 (OR = 4.04; p < 0.001); and Q3 vs. Q4 (OR = 2.65; p < 0.005). The adjusted ORs, with respect to the probabilities of having severe motor difficulties, were the following: Being born in Q4 is 13.03 times more likely than being born in Q1 (p < 0.001); those born in Q3 are 4.85 times more likely than those born in Q1 (p < 0.05); and those born in Q2 4.14 times more than those born in Q1 (p < 0.05). The conclusion is that children born in Q4 are more likely to be classified as children with severe difficulties compared to children born in the other quarters of the same year. metadata Navarro-Patón, Rubén and Pueyo Villa, Silvia and Martín Ayala, Juan Luis and Martí González, Mariacarla and Mecías-Calvo, Marcos mail UNSPECIFIED, silvia.pueyo@uneatlantico.es, juan.martin@uneatlantico.es, mariacarla.marti@uneatlantico.es, marcos.mecias@uneatlantico.es (2021) Is Quarter of Birth a Risk Factor for Developmental Coordinator Disorder in Preschool Children? International Journal of Environmental Research and Public Health, 18 (11). p. 5514. ISSN 1660-4601

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
UNSPECIFIED UNSPECIFIED Cancer stem cells (CSCs) are a rare tumor subpopulation with high differentiation, proliferative and tumorigenic potential compared to the remaining tumor population. CSCs were first discovered by Bonnet and Dick in 1997 in acute myeloid leukemia. The identification and isolation of these cells in this pioneering study were carried out through the flow cytometry, exploiting the presence of specific cell surface molecular markers (CD34+/CD38−). In the following years, different strategies and projects have been developed for the study of CSCs, which are basically divided into surface markers assays and functional assays; some of these techniques also allow working with a cellular model that better mimics the tumor architecture. The purpose of this mini review is to summarize and briefly describe all the current methods used for the identification, isolation and enrichment of CSCs, describing, where possible, the molecular basis, the advantages and disadvantages of each technique with a particular focus on those that offer a three-dimensional culture. metadata Cianciosi, Danila and Ansary, Johura and Forbes-Hernandez, Tamara Y. and Regolo, Lucia and Quinzi, Denise and Gracia Villar, Santos and Garcia Villena, Eduardo and Tutusaus Pifarre, Kilian and Alvarez-Suarez, José M. and Battino, Maurizio and Giampieri, Francesca mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, santos.gracia@uneatlantico.es, eduardo.garcia@uneatlantico.es, kilian.tutusaus@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2021) The Molecular Basis of Different Approaches for the Study of Cancer Stem Cells and the Advantages and Disadvantages of a Three-Dimensional Culture. Molecules, 26 (9). p. 2615. ISSN 1420-3049

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Abierto Inglés Age-related bone disorders such as osteoporosis or osteoarthritis are a major public health problem due to the functional disability for millions of people worldwide. Furthermore, fractures are associated with a higher degree of morbidity and mortality in the long term, which generates greater financial and health costs. As the world population becomes older, the incidence of this type of disease increases and this effect seems notably greater in those countries that present a more westernized lifestyle. Thus, increased efforts are directed toward reducing risks that need to focus not only on the prevention of bone diseases, but also on the treatment of persons already afflicted. Evidence is accumulating that dietary lipids play an important role in bone health which results relevant to develop effective interventions for prevent bone diseases or alterations, especially in the elderly segment of the population. This review focuses on evidence about the effects of dietary lipids on bone health and describes possible mechanisms to explain how lipids act on bone metabolism during aging. Little work, however, has been accomplished in humans, so this is a challenge for future research. metadata Romero-Márquez, Jose M. and Varela-López, Alfonso and Navarro-Hortal, María D. and Badillo-Carrasco, Alberto and Forbes-Hernández, Tamara Y. and Giampieri, Francesca and Dominguez Azpíroz, Irma and Madrigal-Hoyos, Lorena and Battino, Maurizio and Quiles, José L. mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, irma.dominguez@uneatlantico.es, lorena.madrigal@uneatlantico.es, maurizio.battino@uneatlantico.es, jose.quiles@uneatlantico.es (2021) Molecular Interactions between Dietary Lipids and Bone Tissue during Aging. International Journal of Molecular Sciences, 22 (12). p. 6473. ISSN 1422-0067

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Abierto Inglés Down syndrome (DS) or trisomy of chromosome 21 (Hsa21) is characterized by impaired hippocampal-dependent learning and memory. These alterations are due to defective neurogenesis and to neuromorphological and functional anomalies of numerous neuronal populations, including hippocampal granular cells (GCs). It has been proposed that the additional gene dose in trisomic cells induces modifications in nuclear compartments and on the chromatin landscape, which could contribute to some DS phenotypes. The Ts65Dn (TS) mouse model of DS carries a triplication of 92 genes orthologous to those found in Hsa21, and shares many phenotypes with DS individuals, including cognitive and neuromorphological alterations. Considering its essential role in hippocampal memory formation, we investigated whether the triplication of this set of Hsa21 orthologous genes in TS mice modifies the nuclear architecture of their GCs. Our results show that the TS mouse presents alterations in the nuclear architecture of its GCs, affecting nuclear compartments involved in transcription and pre-rRNA and pre-mRNA processing. In particular, the GCs of the TS mouse show alterations in the nucleolar fusion pattern and the molecular assembly of Cajal bodies (CBs). Furthermore, hippocampal GCs of TS mice present an epigenetic dysregulation of chromatin that results in an increased heterochromatinization and reduced global transcriptional activity. These nuclear alterations could play an important role in the neuromorphological and/or functional alterations of the hippocampal GCs implicated in the cognitive dysfunction characteristic of TS mice. metadata Puente-Bedia, Alba and Berciano, María T. and Tapia Martínez, Olga and Martínez-Cué, Carmen and Lafarga, Miguel and Rueda, Noemí mail UNSPECIFIED, UNSPECIFIED, olga.tapia@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2021) Nuclear Reorganization in Hippocampal Granule Cell Neurons from a Mouse Model of Down Syndrome: Changes in Chromatin Configuration, Nucleoli and Cajal Bodies. International Journal of Molecular Sciences, 22 (3). p. 1259. ISSN 1422-0067

Article Subjects > Biomedicine
Subjects > Nutrition
Europe University of Atlantic > Research > Scientific Production Abierto Inglés Modern high-throughput ‘omics’ science tools (including genomics, transcriptomics, proteomics, metabolomics and microbiomics) are currently being applied to nutritional sciences to unravel the fundamental processes of health effects ascribed to particular nutrients in humans and to contribute to more precise nutritional advice. Diet and food components are key environmental factors that interact with the genome, transcriptome, proteome, metabolome and the microbiota, and this life-long interplay defines health and diseases state of the individual. Rheumatoid arthritis (RA) is a chronic autoimmune disease featured by a systemic immune-inflammatory response, in genetically susceptible individuals exposed to environmental triggers, including diet. In recent years increasing evidences suggested that nutritional factors and gut microbiome have a central role in RA risk and progression. The aim of this review is to summarize the main and most recent applications of ‘omics’ technologies in human nutrition and in RA research, examining the possible influences of some nutrients and nutritional patterns on RA pathogenesis, following a nutrigenomics approach. The opportunities and challenges of novel ‘omics technologies’ in the exploration of new avenues in RA and nutritional research to prevent and manage RA will be also discussed. metadata Cassotta, Manuela and Forbes-Hernandez, Tamara Y. and Cianciosi, Danila and Elexpuru Zabaleta, Maria and Sumalla Cano, Sandra and Dominguez Azpíroz, Irma and Bullon, Beatriz and Regolo, Lucia and Alvarez-Suarez, Josè Miguel and Giampieri, Francesca and Battino, Maurizio mail manucassotta@gmail.com, UNSPECIFIED, UNSPECIFIED, maria.elexpuru@uneatlantico.es, sandra.sumalla@uneatlantico.es, irma.dominguez@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, francesca.giampieri@uneatlantico.es, maurizio.battino@uneatlantico.es (2021) Nutrition and Rheumatoid Arthritis in the ‘Omics’ Era. Nutrients, 13 (3). p. 763. ISSN 2072-6643

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Abierto Inglés Context A relationship between obesity and poor oral health has been reported. Objective To investigate the association between overweight/obesity and oral health in Mexican children and adolescents. Data Sources A literature search was conducted of 13 databases and 1 search engine for articles published from 1995 onward. Data Analysis A total of 18 publications were included. Evidence was inconclusive and varied according to sociodemographic factors or outcome measuring tools. The Decayed, Missing, and Filled Teeth and Filled Teeth Surfaces indices and the decayed extracted filled teeth index outcomes were included in a random effects model meta-analysis. Pooled estimates showed no statistically significant oral health differences (measured via the decayed extracted filled teeth or the Decayed, Missing, and Filled Teeth Surfaces indexes) among body mass index (BMI) categories. However, pooled estimates of 6 studies showed that children with higher BMI had worse oral health in permanent teeth (measured via the Decayed Missing Filled Teeth Index) than children with lower BMI (overall mean difference, –0.42; 95%CI, –0.74, –0.11). Conclusion Whether there is an association between poor oral health and high BMI is inconclusive; however, both co-exist among Mexican children. Therefore, health promotion and prevention efforts should address common risk factors and broader risk social determinants shared between noncommunicable diseases. metadata Aceves-Martins, Magaly and Godina-Flores, Naara L and Gutierrez-Gómez, Yareni Yunuen and Richards, Derek and López-Cruz, Lizet and García-Botello, Marcela and Moreno-García, Carlos Francisco mail UNSPECIFIED (2021) Obesity and oral health in Mexican children and adolescents: systematic review and meta-analysis. Nutrition Reviews. ISSN 0029-6643

Article Subjects > Biomedicine
Subjects > Psychology
Europe University of Atlantic > Research > Scientific Production Abierto Inglés Body image (BI) is a trending topic of study since health problems derived from a negative perception of the body are increasing and affecting people of all ages, with an increasing incidence among children from the age of eight. The objective of this study was to evaluate the current perception of the body against the desired body and the degree of body satisfaction of Galician primary education students. A total of 355 students (167 boys (47%)) between 9 and 12 years old participated (mean = 10.53; SD = 0.84). Sociodemographic data (sex, age, height, and weight) were collected, and the Figure Rating Scale was used. There are statistically significant differences between boys and girls in the current perceived figure (p = 0.003) and in the desired figure (p < 0.001). Depending on age, the differences were in current (p = 0.010) and desired (p = 0.021) body perception. In conclusion, boys perceive themselves as having a larger figure than girls do, but this perception is far from reality according to the body mass index. For the desired figure, both boys and girls want to be slimmer, but girls want a slimmer figure. Regarding age, the current perceived figure size increases with age as it increases in those students dissatisfied with their body. metadata Navarro-Patón, Rubén and Mecías-Calvo, Marcos and Pueyo Villa, Silvia and Anaya, Vanessa and Martí-González, Mariacarla and Lago-Ballesteros, Joaquín mail UNSPECIFIED, marcos.mecias@uneatlantico.es, silvia.pueyo@uneatlantico.es, vanessa.anaya@uneatlantico.es, UNSPECIFIED, UNSPECIFIED (2021) Perceptions of the Body and Body Dissatisfaction in Primary Education Children According to Gender and Age. A Cross-Sectional Study. International Journal of Environmental Research and Public Health, 18 (23). p. 12460. ISSN 1660-4601

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Abierto Inglés Therapeutic bacteriophages, commonly called as phages, are a promising potential alternative to antibiotics in the management of bacterial infections of a wide range of organisms including cultured fish. Their natural immunogenicity often induces the modulation of a variated collection of immune responses within several types of immunocytes while promoting specific mechanisms of bacterial clearance. However, to achieve standardized treatments at the practical level and avoid possible side effects in cultivated fish, several improvements in the understanding of their biology and the associated genomes are required. Interestingly, a particular feature with therapeutic potential among all phages is the production of lytic enzymes. The use of such enzymes against human and livestock pathogens has already provided in vitro and in vivo promissory results. So far, the best-understood phages utilized to fight against either Gram-negative or Gram-positive bacterial species in fish culture are mainly restricted to the Myoviridae and Podoviridae, and the Siphoviridae, respectively. However, the current functional use of phages against bacterial pathogens of cultured fish is still in its infancy. Based on the available data, in this review, we summarize the current knowledge about phage, identify gaps, and provide insights into the possible bacterial control strategies they might represent for managing aquaculture-related bacterial diseases. metadata Ramos-Vivas, José and Superio, Joshua and Galindo-Villegas, Jorge and Acosta, Félix mail jose.ramos@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2021) Phage Therapy as a Focused Management Strategy in Aquaculture. International Journal of Molecular Sciences, 22 (19). p. 10436. ISSN 1422-0067

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production UNSPECIFIED UNSPECIFIED The Papillon–Lefèvre syndrome (PLS) is a rare autosomal recessive disorder caused by mutations in the Cathepsin C (CTSC) gene, characterized by periodontitis and palmoplantar hyperkeratosis. The main inflammatory deficiencies include oxidative stress and autophagic dysfunction. Mitochondria are the main source of reactive oxygen species; their impaired function is related to skin diseases and periodontitis. The mitochondrial function has been evaluated in PLS and mitochondria have been targeted as a possible treatment for PLS. We show for the first time an important mitochondrial dysfunction associated with increased oxidative damage of mtDNA, reduced CoQ10 and mitochondrial mass and aberrant morphologies of the mitochondria in PLS patients. Mitochondrial dysfunction, determined by oxygen consumption rate (OCR) in PLS fibroblasts, was treated with CoQ10 supplementation, which determined an improvement in OCR and a remission of skin damage in a patient receiving a topical administration of a cream enriched with CoQ10 0.1%. We provide the first evidence of the role of mitochondrial dysfunction and CoQ10 deficiency in the pathophysiology of PLS and a future therapeutic option for PLS. metadata Castejón-Vega, Beatriz and Battino, Maurizio and Quiles, José L. and Bullon, Beatriz and Cordero, Mario D. and Bullón, Pedro mail UNSPECIFIED, UNSPECIFIED, jose.quiles@uneatlantico.es, UNSPECIFIED, jose.quiles@uneatlantico.es, UNSPECIFIED (2021) Potential Role of the Mitochondria for the Dermatological Treatment of Papillon-Lefèvre. Antioxidants, 10 (1). p. 95. ISSN 2076-3921

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production UNSPECIFIED UNSPECIFIED Plant pathogenic fungi are the largest group of disease-causing agents on crop plants and represent a persistent and significant threat to agriculture worldwide. Conventional approaches based on the use of pesticides raise social concern for the impact on the environment and human health and alternative control methods are urgently needed. The rapid improvement and extensive implementation of RNA interference (RNAi) technology for various model and non-model organisms has provided the initial framework to adapt this post-transcriptional gene silencing technology for the management of fungal pathogens. Recent studies showed that the exogenous application of double-stranded RNA (dsRNA) molecules on plants targeting fungal growth and virulence-related genes provided disease attenuation of pathogens like Botrytis cinerea, Sclerotinia sclerotiorum and Fusarium graminearum in different hosts. Such results highlight that the exogenous RNAi holds great potential for RNAi-mediated plant pathogenic fungal disease control. Production of dsRNA can be possible by using either in-vitro or in-vivo synthesis. In this review, we describe exogenous RNAi involved in plant pathogenic fungi and discuss dsRNA production, formulation, and RNAi delivery methods. Potential challenges that are faced while developing a RNAi strategy for fungal pathogens, such as off-target and epigenetic effects, with their possible solutions are also discussed. metadata Gebremichael, Daniel Endale and Haile, Zeraye Mehari and Negrini, Francesca and Sabbadini, Silvia and Capriotti, Luca and Mezzetti, Bruno and Baraldi, Elena mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, bruno.mezzetti@uneatlantico.es, UNSPECIFIED (2021) RNA Interference Strategies for Future Management of Plant Pathogenic Fungi: Prospects and Challenges. Plants, 10 (4). p. 650. ISSN 2223-7747

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Cerrado Inglés Edible flowers are being used as a new ingredient in modern gastronomy. Recently, these products have also gained interest as an important source of phenolic compounds with potential for biomedical applications. The present work studied a methanolic extract of Rosa x hybrida in which 35 individual phenolic compounds were identified. The extract has been evaluated for its antiproliferative properties in ovarian carcinoma cells. Results showed that the antiproliferative effect was associated with the induction of autophagy and apoptosis with the concomitant ROS increase probably related to mitochondria dysfunction. These antiproliferative effects might be associated with some components of the extract such as quercetin. The extract did not induce damage in healthy cells and that it was able to improve the wound healing activity. The present study also evaluated the properties of the mentioned extract in vivo in C. elegans. Tests demonstrated a lack of toxicity in the worm model. Promising results have been obtained in transgenic strains of C. elegans that produce human beta amyloid peptide, suggesting the possible utility of the extract from the point of view of Alzheimer disease. Altogether, results suggest that Rosa x hybrida extracts could be a new tool for the development of functional foods. metadata Rivas-García, Lorenzo and Quiles, José L. and Roma-Rodrigues, Catarina and Raposo, Luis R. and Navarro-Hortal, María D. and Romero-Márquez, Jose M. and Esteban-Muñoz, Adelaida and Varela-López, Alfonso and García, Laura Carrera and Cianciosi, Danila and Forbes Hernandez, Tamara Y. and Battino, Maurizio and Llopis, Juan and Fernandes, Alexandra R. and Baptista, Pedro V. and Sánchez-González, Cristina mail UNSPECIFIED, jose.quiles@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2021) Rosa x hybrida extracts with dual actions: Antiproliferative effects against tumour cells and inhibitor of Alzheimer disease. Food and Chemical Toxicology, 149. p. 112018. ISSN 0278-6915

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Cerrado Inglés Aim of the study: To assess the prognostic ability of the National Early Warning Score 2 (NEWS2) at three time points of care -at the emergency scene (NEWS2-1), just before starting the transfer by ambulance to the hospital (NEWS2- 2), and at the hospital triage box (NEWS2-3)- to estimate in-hospital mortality after two days since the index event. Methods: Prospective, multicenter, ambulance-based, cohort ongoing study in adults (>18 years) consecutively attended by advanced life support (ALS) and evacuated with high-priority to the emergency departments (ED) between October 2018 and May 2021. Vital sign measures were used to calculate the NEWS2 score at each time point, then this score was entered in a logistic regression model as the single predictor. Two outcomes were considered: first, all-cause mortality of the patients within 2 days of presentation to EMS, and second, unplanned ICU admission. The calibration and scores comparison was performed by representing the predicted vs the observed risk curves according to NEWS score value. Results: 4943 patients were enrolled. Median age was 69 years (interquartile range 53- 81). The NEWS2-3 presented the better performance for all-cause two-day in-hospital mortality with an AUC of 0.941 (95% CI: 0.917-0.964), showing statistical differences with both the NEWS2-1 (0.872 (95% CI: 0.833-0.911); p < 0.003) and with the NEWS2- 2 (0.895 (95% CI: 0.866-0.925; p < 0.05). The calibration and scores comparison results showed that the NEWS2-3 was the best predictive score followed by the NEWS2-2 and the NEWS2-1, respectively. Conclusions: The NEWS2 has an excellent predictive performance. The score showed a very consistent response over time with the difference between “at the emergency scene” and “pre-evacuation” presenting the sharpest change with decreased threshold values, thus displaying a drop in the risk of acute clinical impairment. metadata Martín-Rodríguez, Francisco and Sanz-García, Ancor and Ortega, Guillermo J. and Delgado Benito, Juan F. and Aparicio Obregón, Silvia and Martínez Fernández, Francisco T. and González Crespo, Pilar and Otero de la Torre, Santiago and Castro Villamor, Miguel A. and López-Izquierdo, Raúl mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, silvia.aparicio@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2021) Tracking the National Early Warning Score 2 from prehospital care to the emergency department: A prospective, ambulance-based, observational study. Prehospital Emergency Care. pp. 1-13. ISSN 1090-3127 (Unpublished)

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production UNSPECIFIED UNSPECIFIED The application of metallic nanoparticles (materials with size at least in one dimension ranging from 1 to 100 nm) as a new therapeutic tool will improve the diagnosis and treatment of diseases. The mitochondria could be a therapeutic target to treat pathologies whose origin lies in mitochondrial dysfunctions or whose progression is dependent on mitochondrial function. We aimed to study the subcellular distribution of 2-4 nm iron nanoparticles and its effect on mitochondrial DNA (mtDNA), mitochondrial function, and autophagy in colorectal cell lines (HT-29). Results showed that when cells were exposed to ultra-small iron nanoparticles, their subcellular fate was mainly mitochondria, affecting its respiratory and glycolytic parameters, inducing the migration of the cellular state towards quiescence, and promoting and triggering the autophagic process. These effects support the potential use of nanoparticles as therapeutic agents using mitochondria as a target for cancer and other treatments for mitochondria-dependent pathologies. metadata Rivas-García, Lorenzo and Quiles, José L. and Varela-López, Alfonso and Giampieri, Francesca and Battino, Maurizio and Bettmer, Jörg and Montes-Bayón, María and Llopis, Juan and Sánchez-González, Cristina mail UNSPECIFIED, jose.quiles@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2021) Ultra-Small Iron Nanoparticles Target Mitochondria Inducing Autophagy, Acting on Mitochondrial DNA and Reducing Respiration. Pharmaceutics, 13 (1). p. 90. ISSN 1999-4923

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Cerrado Inglés Corynebacterium striatum is a nosocomial pathogen which is increasingly associated with serious infections in both immunocompetent and immunocompromised patients. However, little is known about virulence factors and mechanisms that may enhance the establishment and long-term survival of Corynebacterium striatum. in the hospital environment. In this study, we investigated the ability of 22 multidrug-resistant C. striatum clinical isolates to adhere to human epithelial cells and to produce biofilm on polystyrene plates, glass and various tracheostomy tubes. We also tested the virulence of these strains on the nematode Caenorhabditis elegans. They showed good adhesion to epithelial human cells after 180 min of infection. The 22 C. striatum were able to produce biofilms on positively and negatively charged abiotic surfaces at 37 °C. They were also able to infect and to kill Caenorhabditis elegans after 5 days of infection. The virulence condition was associated with the presence of SpaDEF operon encoding pili in all strains. This study provides new insights on virulence mechanisms that may contribute to the persistence of C. striatum in the hospital environment, increasing the probability of causing nosocomial infections. metadata Alibi, Sana and Ramos-Vivas, José and Ben Selma, Walid and Ben Mansour, Hedi and Boukadida, Jalel and Navas, Jesús mail UNSPECIFIED, jose.ramos@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2021) Virulence of clinically relevant multidrug resistant Corynebacterium striatum strains and their ability to adhere to human epithelial cells and inert surfaces. Microbial Pathogenesis, 155. p. 104887. ISSN 08824010

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Cerrado Inglés Evidence demonstrates the importance of lipid metabolism and signaling in cancer cell biology. De novo lipogenesis is an important source of lipids for cancer cells, but exogenous lipid uptake remains essential for many cancer cells. Dietary lipids can modify lipids present in tumor microenvironment affecting cancer cell metabolism. Clinical trials have shown that diets rich in polyunsaturated fatty acids (PUFA) can negatively affect tumor growth. However, certain n-6 PUFAs can also contribute to cancer progression. Identifying the molecular mechanisms through which lipids affect cancer progression will provide an opportunity for focused dietary interventions that could translate into the development of personalized diets for cancer control. However, the effective mechanisms of action of PUFAs have not been fully clarified yet. Mitochondria controls ATP generation, redox homeostasis, metabolic signaling, apoptotic pathways and many aspects of autophagy, and it has been recognized to play a key role in cancer. The purpose of this review is to summarize the current evidence linking dietary lipids effects on mitochondrial aspects with consequences for cancer progression and the molecular mechanisms that underlie this association. metadata Varela-López, Alfonso and Vera-Ramírez, Laura and Giampieri, Francesca and Navarro-Hortal, María D. and Forbes-Hernandez, Tamara Y. and Battino, Maurizio and Quiles, José L. mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, jose.quiles@uneatlantico.es (2021) The central role of mitochondria in the relationship between dietary lipids and cancer progression. Seminars in Cancer Biology. ISSN 1044579X

2020

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production UNSPECIFIED UNSPECIFIED Aging is associated with metabolic changes and low-grade inflammation in several organs, which may be due to NLRP3 inflammasome activation. Methods: Here, we asked whether age-related liver changes such as lipid metabolism and fibrosis are reduced in aged mice lacking the NLRP3 inflammasome. We report reduced protein levels of lipid markers (MTP, FASN, DGAT1), SOD activity, oxidative stress marker PTPRG, and the fibrotic markers TPM2β, COL1-α1 associated with increased GATA4, in NLRP3 deficient mice. Fibrotic, lipid, and oxidative reduction in liver tissues of mice was more pronounced in those old KO NLRP3 mice than in the younger ones, despite their greater liver damage. These results suggest that absence of the NLRP3 inflammasome attenuates age-related liver fibrotic pathology in mice, suggesting that pharmacological targeting may be beneficial. metadata Gallego, Paloma and Castejón-Vega, Beatriz and del Campo, José A. and Cordero, Mario D. mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, mario.cordero@uneatlantico.es (2020) The Absence of NLRP3-inflammasome Modulates Hepatic Fibrosis Progression, Lipid Metabolism, and Inflammation in KO NLRP3 Mice during Aging. Cells, 9 (10). p. 2148. ISSN 2073-4409

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Cerrado Inglés Aging is the major risk factor for many metabolic chronic diseases. Several metabolic pathways suffer a progressive impairment during aging including body composition and insulin resistance which are associated to autophagy dysfunction and increased inflammation. Many of these alterations are aggravated by non-healthy lifestyle such as obesity and hypercaloric diet which have been shown to accelerate aging. Here, we show that the deleterious effect of hypercaloric diets is reverted by the NLRP3 inflammasome inhibition. NLRP3 deficiency extends mean lifespan of adult mice fed a high-fat diet. This lifespan extension is accompanied by metabolic health benefits including reduced liver steatosis and cardiac damage, improved glucose and lipid metabolism, and improved protein expression profiles of SIRT-1, mTOR, autophagic flux, and apoptosis. These findings suggest that the suppression of NLRP3 prevented many age-associated changes in metabolism impaired by the effect of hypercaloric diets. metadata Cañadas-Lozano, Diego and Marín-Aguilar, Fabiola and Castejón-Vega, Beatriz and Ryffel, Bernhard and Navarro-Pando, José M. and Ruiz-Cabello, Jesús and Alcocer-Gómez, Elísabet and Bullón, Pedro and Cordero, Mario D. mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, jose.navarro@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, mario.cordero@uneatlantico.es (2020) Blockade of the NLRP3 inflammasome improves metabolic health and lifespan in obese mice. GeroScience, 42 (2). pp. 715-725. ISSN 2509-2715

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production UNSPECIFIED UNSPECIFIED ncreasing evidence connects periodontitis with a variety of systemic diseases, including metabolic syndrome, atherosclerosis, and non-alcoholic fatty liver disease (NAFLD). The proposal of this study was to evaluate the role of diets rich in saturated fat and cholesterol in some aspects of periodontal diseases in a lipopolysaccharide (LPS)-induced model of periodontal disease in rabbits and to assess the influence of a periodontal intervention on hyperlipidemia, atherosclerosis, and NAFLD progression to non-alcoholic steatohepatitis. Male rabbits were maintained on a commercial standard diet or a diet rich in saturated fat (3% lard w/w) and cholesterol (1.3% w/w) (HFD) for 40 days. Half of the rabbits on each diet were treated 2 days per week with intragingival injections of LPS from Porphyromonas gingivalis. Morphometric analyses revealed that LPS induced higher alveolar bone loss (ABL) around the first premolar in animals receiving standard diets, which was exacerbated by the HFD diet. A higher score of acinar inflammation in the liver and higher blood levels of triglycerides and phospholipids were found in HFD-fed rabbits receiving LPS. These results suggest that certain dietary habits can exacerbate some aspects of periodontitis and that bad periodontal health can contribute to dyslipidemia and promote NAFLD progression, but only under certain conditions. metadata Varela-López, Alfonso and Bullón, Pedro and Ramírez-Tortosa, César L. and Navarro-Hortal, María D. and Robles-Almazán, María and Bullón, Beatriz and Cordero, Mario D. and Battino, Maurizio and Quiles, José L. mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, mario.cordero@uneatlantico.es, UNSPECIFIED, UNSPECIFIED (2020) A Diet Rich in Saturated Fat and Cholesterol Aggravates the Effect of Bacterial Lipopolysaccharide on Alveolar Bone Loss in a Rabbit Model of Periodontal Disease. Nutrients, 12 (5). p. 1405. ISSN 2072-6643

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Cerrado Inglés The consumption of edible flowers has increased in recent years because of their wide use in gastronomy as a decorative element or as an ingredient in dishes. In addition to influencing texture, taste or appearance, flowers are rich in bioactive compounds. Scope and approach This review focuses on the composition and nutritional features of edible flowers and their extracts, and their health benefits related to cardiovascular diseases, cancer, neurological diseases, diabetes, obesity, in addition to their hepatoprotective and microbicidal effects. The mechanisms though which some of them exert their effects and the specific compounds associated with these effects have also been addressed. Key findings and conclusions Edible flowers have a high content in phenolic compounds and a high antioxidant capacity, property that confers positive effects on oxidative stress-related diseases. Some extracts based on edible flowers exert hepato-, neuro- or cardioprotective actions. Anticancer properties, improvements in metabolic disorders and microbiocidal effects even in multidrug-resistant bacteria have also been attributed to some edible flowers or their extracts. Most of the studies have been performed in vitro, so further assays in in vivo models are needed. Additionally, it would be important to elucidate the mechanisms by which these observed effects are performed. In conclusion, edible flowers could be used as a new approach for the development of nutraceutical products or functional foods. metadata Rivas-García, Lorenzo and Navarro-Hortal, María D. and Romero-Márquez, José M. and Forbes-Hernández, Tamara Y. and Varela-López, Alfonso and Llopis, Juan and Sánchez-González, Cristina and Quiles, José L. mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, jose.quiles@uneatlantico.es (2020) Edible flowers as a health promoter: An evidence-based review. Trends in Food Science & Technology. ISSN 0924-2244

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Universidad Internacional do Cuanza > Research > Scientific Production
Cerrado Inglés Fasting, caloric restriction and foods or compounds mimicking the biological effects of caloric restriction, known as caloric restriction mimetics, have been associated with a lower risk of age-related diseases, including cardiovascular diseases, cancer and cognitive decline, and a longer lifespan. Reduced calorie intake has been shown to stimulate cancer immunosurveillance, reducing the migration of immunosuppressive regulatory T cells towards the tumor bulk. Autophagy stimulation via reduction of lysine acetylation, increased sensitivity to chemo- and immunotherapy, along with a reduction of insulin-like growth factor 1 and reactive oxygen species have been described as some of the major effects triggered by caloric restriction. Fasting and caloric restriction have also been shown to beneficially influence gut microbiota composition, modify host metabolism, reduce total cholesterol and triglyceride levels, lower diastolic blood pressure and elevate morning cortisol level, with beneficial modulatory effects on cardiopulmonary fitness, body fat and weight, fatigue and weakness, and general quality of life. Moreover, caloric restriction may reduce the carcinogenic and metastatic potential of cancer stem cells, which are generally considered responsible of tumor formation and relapse. Here, we reviewed in vitro and in vivo studies describing the effects of fasting, caloric restriction and some caloric restriction mimetics on immunosurveillance, gut microbiota, metabolism, and cancer stem cell growth, highlighting the molecular and cellular mechanisms underlying these effects. Additionally, studies on caloric restriction interventions in cancer patients or cancer risk subjects are discussed. Considering the promising effects associated with caloric restriction and caloric restriction mimetics, we think that controlled-randomized large clinical trials are warranted to evaluate the inclusion of these non-pharmacological approaches in clinical practice. metadata Pistollato, Francesca and Forbes-Hernández, Tamara Y. and Calderón Iglesias, Rubén and Ruiz Salces, Roberto and Elexpuru Zabaleta, Maria and Dominguez Azpíroz, Irma and Cianciosi, Danila and Quiles, José L. and Giampieri, Francesca and Battino, Maurizio mail francesca.pistollato@uneatlantico.es, UNSPECIFIED, ruben.calderon@uneatlantico.es, roberto.ruiz@uneatlantico.es, maria.elexpuru@uneatlantico.es, irma.dominguez@unini.edu.mx, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2020) Effects of caloric restriction on immunosurveillance, microbiota and cancer cell phenotype: Possible implications for cancer treatment. Seminars in Cancer Biology. ISSN 1044-579X

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Abierto Inglés There is a huge interest in developing novel hollow fiber (HF) membranes able to modulate neural differentiation to produce in vitro blood–brain barrier (BBB) models for biomedical and pharmaceutical research, due to the low cell-inductive properties of the polymer HFs used in current BBB models. In this work, poly(ε-caprolactone) (PCL) and composite PCL/graphene (PCL/G) HF membranes were prepared by phase inversion and were characterized in terms of mechanical, electrical, morphological, chemical, and mass transport properties. The presence of graphene in PCL/G membranes enlarged the pore size and the water flux and presented significantly higher electrical conductivity than PCL HFs. A biocompatibility assay showed that PCL/G HFs significantly increased C6 cells adhesion and differentiation towards astrocytes, which may be attributed to their higher electrical conductivity in comparison to PCL HFs. On the other hand, PCL/G membranes produced a cytotoxic effect on the endothelial cell line HUVEC presumably related with a higher production of intracellular reactive oxygen species induced by the nanomaterial in this particular cell line. These results prove the potential of PCL HF membranes to grow endothelial cells and PCL/G HF membranes to differentiate astrocytes, the two characteristic cell types that could develop in vitro BBB models in future 3D co-culture systems. metadata Mantecón-Oria, Marián and Diban, Nazely and Berciano, Maria T. and Rivero, Maria J. and David, Oana and Lafarga, Miguel and Tapia Martínez, Olga and Urtiaga, Ane mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, olga.tapia@uneatlantico.es, UNSPECIFIED (2020) Hollow Fiber Membranes of PCL and PCL/Graphene as Scaffolds with Potential to Develop In Vitro Blood—Brain Barrier Models. Membranes, 10 (8). p. 161. ISSN 2077-0375

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production UNSPECIFIED UNSPECIFIED UNSPECIFIED metadata Cordero, Mario D. and Ruiz-Cabello, Jesús mail mario.cordero@uneatlantico.es, UNSPECIFIED (2020) Inflamm-ageing or inflammasom-ageing as independent events. Aging, 12 (18). pp. 17759-17760. ISSN 1945-4589

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Cerrado Inglés Primary ovarian insufficiency (POI) is a critical fertility defect characterized by anticipated impairment of the follicular reserve, which pathophysiological mechanisms have not yet been identified. We have investigated the possible relationship between AMP-activated protein kinase (AMPK) and the pathophysiology of POI. We studied 35 POI patients with altered levels of follicle-stimulating hormone (FSH) and estradiol and increased percentage of overweight compared with 20 healthy women. Blood mononuclear cells from POI patients showed reduced levels of phosphorylated AMPK, adenosine triphosphate, and high serum levels of leptin and tumor necrosis factor-alpha. All these alterations were also observed in granulosa cells, a more closer environment of the ovary. Significant negative correlations between AMPK and body mass index, FSH and leptin levels, and a positive correlation between AMPK and estradiol levels were observed. Oral metformin supplementation restored biochemical parameters. Interestingly, AMPK levels from patients were negatively correlated with age, suggesting an accelerated aging in POI mediated by the impairment of AMPK. These results lead to the hypothesis that an impairment of AMPK could be implicated in the metabolic pathophysiology of POI indicating at AMPK induction as a possible new therapeutic target. metadata Navarro-Pando, José M. and Bullón, Pedro and Cordero, Mario D. and Alcocer-Gómez, Elísabet mail jose.navarro@uneatlantico.es, UNSPECIFIED, mario.cordero@uneatlantico.es, UNSPECIFIED (2020) Is AMP-Activated Protein Kinase Associated to the Metabolic Changes in Primary Ovarian Insufficiency? Antioxidants & Redox Signaling, 33 (15). pp. 1115-1121. ISSN 1523-0864

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Cerrado Inglés Sir, In their recently published study, Yaron et al. (2020) expose an interesting strategy based in cfDNA testing (cfDNA-T) which could serve as an alternative to cytogenetic analysis in products of conceptions in recurrent pregnancy loss and could guide further management of this medical topic; however, in our opinion, some important aspects should be considered. Firstly, Illanes et al. (2007) found that cfDNA concentration in the maternal blood can be detected from Week 4 of gestation but it is only reliable from Week 7. However, others authors’ experience could be different. Clark-Ganheart et al. (2015) recommend cfDNA analysis only after 8 weeks of gestation. Zeevi et al. (2018) reported that aneuploidy screening by cfDNA is not generally accurate before Week 8, leading to a period in pregnancy (namely, Weeks 4 through 8) during which assessment of the genetic status of the embryo is not generally feasible. Galeva et al.... metadata Marcos Rodríguez, Ana Teresa and Navarro-Pando, José Manuel mail anateresa.marcos@uneatlantico.es, jose.navarro@uneatlantico.es (2020) Letter: cfDNA testing in recurrent pregnancy loss: a new step in the right way but still raw for the clinical area. Human Reproduction, 36 (3). pp. 827-829. ISSN 0268-1161

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Cerrado Inglés pinal muscular atrophy (SMA) is caused by a deletion or mutation of the survival motor neuron 1 (SMN1) gene. Reduced SMN levels lead to motor neuron degeneration and muscular atrophy. SMN protein localizes to the cytoplasm and Cajal bodies. Moreover, in myofibrils from Drosophila and mice, SMN is a sarcomeric protein localized to the Z-disc. Although SMN participates in multiple functions, including the biogenesis of spliceosomal small nuclear ribonucleoproteins, its role in the sarcomere is unclear. Here, we analyzed the sarcomeric organization of SMN in human control and type I SMA skeletal myofibers. In control sarcomeres, we demonstrate that human SMN is localized to the titin-positive M-band and actin-positive I-band, and to SMN-positive granules that flanked the Z-discs. Co-immunoprecipitation assays revealed that SMN interacts with the sarcomeric protein actin, α-actinin, titin, and profilin2. In the type I SMA muscle, SMN levels were reduced, and atrophic (denervated) and hypertrophic (nondenervated) myofibers coexisted. The hypertrophied myofibers, which are potential primary targets of SMN deficiency, exhibited sites of focal or segmental alterations of the actin cytoskeleton, where the SMN immunostaining pattern was altered. Moreover, SMN was relocalized to the Z-disc in overcontracted minisarcomeres from hypertrophic myofibers. We propose that SMN could have an integrating role in the molecular components of the sarcomere. Consequently, low SMN levels might impact the normal sarcomeric architecture, resulting in the disruption of myofibrils found in SMA muscle. This primary effect might be independent of the neurogenic myopathy produced by denervation and contribute to pathophysiology of the SMA myopathy. metadata Berciano, María T. and Castillo-Iglesias, María S. and Val-Bernal, J. Fernando and Lafarga, Vanesa and Rodriguez-Rey, José C. and Lafarga, Miguel and Tapia Martínez, Olga mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, olga.tapia@uneatlantico.es (2020) Mislocalization of SMN from the I-band and M-band in human skeletal myofibers in spinal muscular atrophy associates with primary structural alterations of the sarcomere. Cell and Tissue Research, 381 (3). pp. 461-478. ISSN 0302-766X

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Cerrado Inglés The heart pumps blood throughout the whole life of an organism, without rest periods during which to replenish energy or detoxify. Hence, cardiomyocytes, the working units of the heart, have mechanisms to ensure constitutive production of energy and detoxification to preserve fitness and function for decades. Even more challenging, the heart must adapt to the varying conditions of the organism from fetal life to adulthood, old age, and pathological stress. Mitochondria are at the nexus of these processes by producing not only energy but also metabolites and oxidative byproducts that can activate alarm signals and be toxic to the cell. We review basic concepts about cardiac mitochondria with a focus on their remarkable adaptations, including elimination, throughout the mammalian lifetime. metadata Sánchez-Díaz, María and Nicolás-Ávila, José Ángel and Cordero, Mario D. and Hidalgo, Andrés mail UNSPECIFIED, UNSPECIFIED, mario.cordero@uneatlantico.es, UNSPECIFIED (2020) Mitochondrial Adaptations in the Growing Heart. Trends in Endocrinology & Metabolism, 31 (4). pp. 308-319. ISSN 1043-2760

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production UNSPECIFIED UNSPECIFIED Inflammation is a hallmark of aging and accelerated aging syndromes. In this context, inflammation has been associated to the pathophysiology of Hutchinson–Gilford progeria syndrome (HGPS). In this study, we report that progeroid skin fibroblasts and animal models present an hyperactivation of the NLRP3-inflammasome complex. High expression of NLRP3 and caspase 1 was also observed in skin fibroblasts from HGPS associated to the nuclei morphology. Lymphoblast from HGPS also showed increased basal levels of NLRP3 and caspase 1 independent to the induction from metabolic factors. Consistent with these results, Zmpste24−/− showed high expression of Nlrp3 and caspase 1 in heart, liver and kidney and reduced levels of Nlrc3, however these changes were not observed in other inflammasomes. We also show that pharmacological inhibition of NLRP3 using a direct NLRP3 inhibitor, MCC950, improved cellular phenotype, significantly extends the lifespan of these progeroid animals and reduced inflammasome-dependent inflammation. These findings suggest the NLRP3-inflammasome comples as a therapeutic approach for patients with HGPS. metadata Alcocer-Gómez, Elísabet and Castejón-Vega, Beatriz and Nuñez-Vasco, Jéssica and Lendines-Cordero, Débora and Navarro-Pando, José M. and Cordero, Mario D. mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, jose.navarro@uneatlantico.es, mario.cordero@uneatlantico.es (2020) NLRP3 inflammasome inhibition rescues Hutchinson-Gilford Progeria cellular phenotype and extend longevity of an animal model.

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Abierto Inglés Cardiomyocytes are subjected to the intense mechanical stress and metabolic demands of the beating heart. It is unclear whether these cells, which are long-lived and rarely renew, manage to preserve homeostasis on their own. While analyzing macrophages lodged within the healthy myocardium, we discovered that they actively took up material, including mitochondria, derived from cardiomyocytes. Cardiomyocytes ejected dysfunctional mitochondria and other cargo in dedicated membranous particles reminiscent of neural exophers, through a process driven by the cardiomyocyte’s autophagy machinery that was enhanced during cardiac stress. Depletion of cardiac macrophages or deficiency in the phagocytic receptor Mertk resulted in defective elimination of mitochondria from the myocardial tissue, activation of the inflammasome, impaired autophagy, accumulation of anomalous mitochondria in cardiomyocytes, metabolic alterations, and ventricular dysfunction. Thus, we identify an immune-parenchymal pair in the murine heart that enables transfer of unfit material to preserve metabolic stability and organ function. metadata Nicolás-Ávila, José A. and Lechuga-Vieco, Ana V. and Esteban-Martínez, Lorena and Sánchez-Díaz, María and Díaz-García, Elena and Santiago, Demetrio J. and Rubio-Ponce, Andrea and Li, Jackson LiangYao and Balachander, Akhila and Quintana, Juan A. and Martínez-de-Mena, Raquel and Castejón-Vega, Beatriz and Pun-García, Andrés and Través, Paqui G. and Bonzón-Kulichenko, Elena and García-Marqués, Fernando and Cussó, Lorena and A-González, Noelia and González-Guerra, Andrés and Roche-Molina, Marta and Martin-Salamanca, Sandra and Crainiciuc, Georgiana and Guzmán, Gabriela and Larrazabal, Jagoba and Herrero-Galán, Elías and Alegre-Cebollada, Jorge and Lemke, Greg and Rothlin, Carla V. and Jimenez-Borreguero, Luis Jesús and Reyes, Guillermo and Castrillo, Antonio and Desco, Manuel and Muñoz-Cánoves, Pura and Ibáñez, Borja and Torres, Miguel and Ng, Lai Guan and Priori, Silvia G. and Bueno, Héctor and Vázquez, Jesús and Cordero, Mario D. and Bernal, Juan A. and Enríquez, José A. and Hidalgo, Andrés mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, mario.cordero@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2020) A Network of Macrophages Supports Mitochondrial Homeostasis in the Heart. Cell, 183 (1). 94-109.e23. ISSN 0092-8674

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Cerrado Inglés Alzheimer’s disease (AD) is a progressive neurodegenerative pathology affecting milions of people worldwide associated with deposition of senile plaques. While the genetic and environmental risk factors associated with the onset and consolidation of late onset AD are heterogeneous and sporadic, growing evidence also suggests a potential link between some infectious diseases caused by oral microbiota and AD. Oral microbiota dysbiosis is purported to contribute either directly to amyloid protein production, or indirectly to neuroinflammation, occurring as a consequence of bacterial invasion. Over the last decade, the development of Human Oral Microbiome database (HOMD) has deepened our understanding of oral microbes and their different roles during the human lifetime. Oral pathogens mostly cause caries, periodontal disease, and edentulism in aged population, and, in particular, alterations of the oral microbiota causing chronic periodontal disease have been associated with the risk of AD. Here we describe how different alterations of the oral microbiota may be linked to AD, highlighting the importance of a good oral hygiene for the prevention of oral microbiota dysbiosis. metadata Sureda, Antoni and Daglia, Maria and Argüelles Castilla, Sandro and Sanadgol, Nima and Fazel Nabavi, Seyed and Khan, Haroon and Belwal, Tarun and Jeandet, Philippe and Marchese, Anna and Pistollato, Francesca and Forbes-Hernandez, Tamara and Battino, Maurizio and Berindan-Neagoe, Ioana and D’Onofrio, Grazia and Nabavi, Seyed Mohammad mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, francesca.pistollato@uneatlantico.es, UNSPECIFIED, maurizio.battino@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2020) Oral microbiota and Alzheimer’s disease: Do all roads lead to Rome? Pharmacological Research, 151. p. 104582. ISSN 10436618

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Cerrado Inglés In the last decade, the prevalence of autism spectrum disorders (ASD) has dramatically escalated worldwide. Currently available drugs mainly target some co-occurring symptoms of ASD, but are not effective on the core symptoms, namely impairments in communication and social interaction, and the presence of restricted and repetitive behaviors. On the other hand, transplantation of hematopoietic and mesenchymal stem cells in ASD children has been shown promising to stimulate the recruitment, proliferation, and differentiation of tissue-residing native stem cells, reducing inflammation, and improving some ASD symptoms. Moreover, several comorbidities have also been associated with ASD, such as immune dysregulation, gastrointestinal issues and gut microbiota dysbiosis. Non-pharmacological approaches, such as dietary supplementations with certain vitamins, omega-3 polyunsaturated fatty acids, probiotics, some phytochemicals (e.g., luteolin and sulforaphane), or overall diet interventions (e.g., gluten free and casein free diets) have been considered for the reduction of such comorbidities and the management of ASD. Here, interventional studies describing pharmacological and non-pharmacological treatments in ASD children and adolescents, along with stem cell-based therapies, are reviewed. metadata Pistollato, Francesca and Forbes-Hernandez, Tamara Y. and Calderón Iglesias, Rubén and Ruiz Salces, Roberto and Elexpuru Zabaleta, Maria and Cianciosi, Danila and Giampieri, Francesca and Battino, Maurizio mail francesca.pistollato@uneatlantico.es, UNSPECIFIED, ruben.calderon@uneatlantico.es, roberto.ruiz@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2020) Pharmacological, non-pharmacological and stem cell therapies for the management of autism spectrum disorders: A focus on human studies. Pharmacological Research, 152. p. 104579. ISSN 1043-6618

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Abierto Inglés Amyotrophic lateral sclerosis (ALS) might not only be circumscribed to the motor system but also involves other neuronal systems including sensory abnormalities. In line with this notion, we aimed to assess the pathophysiology of sensory disturbances in the SOD1G93A mouse model of ALS, focusing on the satellite glial cells (SGCs) at the dorsal root ganglion (DRG) as a new potential target of the disease. metadata Ruiz-Soto, María and Riancho, Javier and Tapia Martínez, Olga and Lafarga, Miguel and Berciano, María T. mail UNSPECIFIED, UNSPECIFIED, olga.tapia@uneatlantico.es, UNSPECIFIED, UNSPECIFIED (2020) Satellite Glial Cells of the Dorsal Root Ganglion: A New “Guest/Physiopathological Target” in ALS. Frontiers in Aging Neuroscience, 12. ISSN 1663-4365

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production UNSPECIFIED UNSPECIFIED Introduction: Inflammasomes are recognized as key components of the innate immune response in sepsis. We aimed to describe the transcriptional expression of nucleotide-binding domain, leucine-rich repeat–containing receptor, pyrin domain–containing-3 (NLRP3), and serum interleukin-1β (IL-1 β) in critically ill patients, their changes over the first week and their prognostic value in septic patients. Methods: Prospective study including patients with sepsis based on Sepsis-3 definitions and a control group of critically ill patients without sepsis. We measured the circulating levels of IL-1β as well as the transcriptional expression of NLRP3 at admission and on days 3 and 7. Caspase-1 and caspase-3 activation was analyzed in a matched cohort of patients with septic shock (four dead and four survivors). Results: Fifty-five septic patients and 11 non-septic patients were studied. Levels on day 0 and 3 of IL-1 β and NLRP3 inflammasome expression were significantly higher in patients with sepsis than in controls. NLRP3 was significantly higher in septic patients who survived at day 7 without significant difference between survivors and non-survivors at baseline and on day 3. In survivors, an increased caspase-1 protein expression with reduced expression caspase-3 was observed with the opposite pattern in those who died. Conclusions: NLRP3 is activated in critically ill patients but this up-regulation is more intense in patients with sepsis. In sepsis, a sustained NLRP3 activation during the first week is protective and sepsis. An increased caspase-1 protein expression with reduced expression caspase-3 is the pattern observed in septic shock patients who survive. metadata Garnacho-Montero, José and Palacios-García, Inmaculada and Díaz-Martín, Ana and Gutiérrez-Pizarraya, Antonio and López-Sánchez, José M. and Gómez, Elísabet Alcocer and Cordero, Mario D. mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, mario.cordero@uneatlantico.es (2020) Sequential Changes of NLRP3 Inflammasome Activation in Sepsis and its Relationship With Death. Shock, 54 (3). pp. 294-300. ISSN 1073-2322

Article Subjects > Biomedicine
Subjects > Psychology
Europe University of Atlantic > Research > Scientific Production Abierto Inglés Background: In recent years, ultrasonic vocalizations (USV) in pups has become established as a good tool for evaluating behaviors related to communication deficits and emotional states observed in autism spectrum disorder (ASD). Prenatal valproic acid (VPA) exposure leads to impairments and social behavior deficits associated with autism, with the effects of VPA being considered as a reliable animal model of ASD. Some studies also suggest that prenatal exposure to chlorpyrifos (CPF) could enhance autistic-like behaviors. Methods: In order to explore these similarities, in the present study we tested whether prenatal exposure to CPF at GD12.5–14.5 produces effects that are comparable to those produced by prenatal VPA exposure at GD12.5 in infant Wistar rats. Using Deep Squeek software, we evaluated total number of USVs, latency to the first call, mean call duration, principal frequency peak, high frequency peak, and type of calls. Results: Consistent with our hypothesis, we found that exposure to both CPF and VPA leads to a significantly smaller number of calls along with a longer latency to produce the first call. No significant effects were found for the remaining dependent variables. Conclusions: These results suggest that prenatal exposure to CPF could produce certain behaviors that are reminiscent of those observed in ASD patients metadata Morales-Navas, Miguel and Castaño-Castaño, Sergio and Pérez-Fernández, Cristian and Sánchez-Gil, Ainhoa and Teresa Colomina, María and Leinekugel, Xavier and Sánchez-Santed, Fernando mail UNSPECIFIED, sergio.castano@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2020) Similarities between the Effects of Prenatal Chlorpyrifos and Valproic Acid on Ultrasonic Vocalization in Infant Wistar Rats. International Journal of Environmental Research and Public Health, 17 (17). p. 6376. ISSN 1660-4601

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production UNSPECIFIED UNSPECIFIED Background Distal motor neuropathies with a genetic origin have a heterogeneous clinical presentation with overlapping features affecting distal nerves and including spinal muscular atrophies and amyotrophic lateral sclerosis. This indicates that their genetic background is heterogeneous. Patient and methods In this work, we have identified and characterized the genetic and molecular base of a patient with a distal sensorimotor neuropathy of unknown origin. For this study, we performed whole-exome sequencing, molecular modelling, cloning and expression of mutant gene, and biochemical and cell biology analysis of the mutant protein. Results A novel homozygous recessive mutation in the human VRK1 gene, coding for a chromatin kinase, causing a substitution (c.637T > C; p.Tyr213His) in exon 8, was detected in a patient presenting since childhood a progressive distal sensorimotor neuropathy and spinal muscular atrophy syndrome, with normal intellectual development. Molecular modelling predicted this mutant VRK1 has altered the kinase activation loop by disrupting its interaction with the C-terminal regulatory region. The p.Y213H mutant protein has a reduced kinase activity with different substrates, including histones H3 and H2AX, proteins involved in DNA damage responses, such as p53 and 53BP1, and coilin, the scaffold for Cajal bodies. The mutant VRK1(Y213H) protein is unable to rescue the formation of Cajal bodies assembled on coilin, in the absence of wild-type VRK1. Conclusion The VRK1(Y213H) mutant protein alters the activation loop, impairs the kinase activity of VRK1 causing a functional insufficiency that impairs the formation of Cajal bodies assembled on coilin, a protein that regulates SMN1 and Cajal body formation. metadata Marcos, Ana T. and Martín‐Doncel, Elena and Morejón‐García, Patricia and Marcos‐Alcalde, Iñigo and Gómez‐Puertas, Paulino and Segura‐Puimedon, María and Armengol, Lluis and Navarro‐Pando, José M. and Lazo, Pedro A. mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, jose.navarro@uneatlantico.es, UNSPECIFIED (2020) VRK1 (Y213H) homozygous mutant impairs Cajal bodies in a hereditary case of distal motor neuropathy. Annals of Clinical and Translational Neurology, 7 (5). pp. 808-818. ISSN 2328-9503

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production UNSPECIFIED UNSPECIFIED Background αB-crystallin is a promiscuous protein involved in numerous cell functions. Mutations in CRYAB have been found in patients with different pathological phenotypes that are not properly understood. Patients can present different diseases like cataracts, muscle weakness, myopathy, cardiomyopathy, respiratory insufficiency or dysphagia, but also a variable combination of these pathologies has been found. These mutations can show either autosomal dominant or recessive mode of inheritance and variable penetrance and expressivity. This is the first report of congenital cataracts and myopathy described in childhood due to a CRYAB mutation with autosomal dominant mode of inheritance. Methods The whole exome sequence was subjected to phenotype-driven analysis and a novel variant in CRYAB was detected: c.514delG, p.(Ala172ProfsTer14). The mutation was located in the C-terminal domain of the protein, which is essential for chaperone activity. The deduced protein was analyzed searching for alterations of the relevant physico-chemical properties described for this domain. A muscle biopsy was also tested for CRYAB with immunohistochemical and histoenzymatic techniques. Results CRYAB displayed a mild immunoreactivity in the subsarcolemmal compartment with no pathological sarcoplasmic accumulation. It agrees with an alteration of the physico-chemical properties predicted for the C-terminal domain: hydrophobicity, stiffness, and isomerization. Conclusions The described mutation leads to elongation of the protein at the carboxi-terminal domain (CTD) with altered properties, which are essential for solubility and activity. It suggests that can be the cause of the severe conditions observed in this patient. metadata Marcos Rodríguez, Ana Teresa and Amorós, Diego and Muñoz-Cabello, Beatriz and Galán, Francisco and Rivas Infante, Eloy and Alcaraz‐Mas, Luis and Navarro‐Pando, José M. mail anateresa.marcos@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, jose.navarro@uneatlantico.es (2020) A novel dominant mutation inCRYABgene leading to a severe phenotype with childhood onset. Molecular Genetics & Genomic Medicine, 8 (8). e1290. ISSN 2324-9269

2019

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Cerrado Inglés About 1 of 10 women, particularly those older than 60 years of age, shows some degree of thyroid hormone deficiency. Thyroid diseases are generally characterized by perturbations of thyroid signaling homeostasis. The most common examples of thyroid diseases include hypothyroidism, hyperthyroidism, and several types of thyroid cancers. Phytochemicals have been shown to have either beneficial or detrimental effects on thyroid function. Some flavonoids have been reported to affect the expression and the activity of several thyroid-related enzymes and proteins, and for this reason some concerns have been raised about the possible thyroid-disruptive properties of foods enriched in these substances. On the other hand, the beneficial effects of some plant-derived compounds, such as myricetin, quercetin, apigenin, rutin, genistein, and curcumin, and their possible role as adjuvants for the treatment of thyroid cancers have been described. Here, the role of phytochemicals in thyroid signaling modulation and their possible beneficial or detrimental effects on thyroid disease risk are discussed. metadata Pistollato, Francesca and Masías Vergara, Manuel and Agudo-Toyos, Pablo and Giampieri, Francesca and Battino, Maurizio mail francesca.pistollato@uneatlantico.es, manuel.masias@uneatlantico.es, pablo.agudo@uenatlantico.es, UNSPECIFIED, UNSPECIFIED (2019) Effects of phytochemicals on thyroid function and their possible role in thyroid disease. Annals of the New York Academy of Sciences, 1443 (1). pp. 3-19. ISSN 0077-8923

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Abierto Inglés The NLRP3 inflammasome has emerged as an important regulator of metabolic disorders and age-related diseases in NLRP3-deficient mice. In this article, we determine whether, in old mice C57BL6J, the NLRP3 inflammasome inhibitor MCC950 is able to attenuate age-related metabolic syndrome to providing health benefits. We report that MCC950 attenuates metabolic and hepatic dysfunction in aged mice. In addition, MCC950 inhibited the Pi3K/AKT/mTOR pathway, enhanced autophagy, and activated peroxisome proliferator-activated receptor-α in vivo and in vitro. The data suggest that MCC950 mediates the protective effects by the mammalian target of rapamycin inhibition, thus activating autophagy and peroxisome proliferator-activated receptor-α. In conclusion, pharmacological inhibition of NLRP3 in aged mice has a significant impact on health. Thus, NLRP3 may be a therapeutic target of human age-related metabolic syndrome. metadata Anderson, Rozalyn and Cordero, Mario D. and Bullón, Pedro and Ruiz-Cabello, Jesús and Robertson, Avril A B and Ryffel, Bernhard and Pérez-Pulido, Antonio J and Muntané, Jordi and Pérez-Alegre, Mónica and Andújar-Pulido, Eloísa and de la Cruz, Patricia and Cooper, Matthew A and Lendines-Cordero, Debora and Alcocer-Gómez, Elísabet and Castejón-Vega, Beatriz and Marín-Aguilar, Fabiola mail UNSPECIFIED, mario.cordero@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2019) NLRP3 Inflammasome Inhibition by MCC950 in Aged Mice Improves Health via Enhanced Autophagy and PPARα Activity. The Journals of Gerontology: Series A, 75 (8). pp. 1457-1464. ISSN 1079-5006

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Cerrado Inglés Amblyopia or lazy eye is a neurodevelopmental disorder that arises during the infancy and is caused by the interruption of binocular sensory activity before maturation of the nervous system. This impairment causes long-term deterioration of visual skills, particularly visual acuity and depth perception. Although visual function recovery has been supposed to be decreased with age as consequence of reduced neuronal plasticity, recent studies have shown that it is possible to promote plasticity and neurorestoration in the adult brain. Thus, transcranial direct current stimulation (tDCS) has been shown effective to treat amblyopia in the adulthood. In the present work we used postnatal monocular deprivation in Long Evans rats as an experimental model of amblyopia and the cliff test task to assess depth perception. Functional brain imaging PET was used to assess the effect of tDCS on cortical and subcortical activity. Visually deprived animals ability to perceive depth in the cliff test was significantly reduced in comparison to their controls. However, after 8 sessions of tDCS applied through 8 consecutive days, depth perception of amblyopic treated animals improved reaching control level. PET data showed 18F-FDG uptake asymmetries in the visual cortex of amblyopic animals, which disappeared after tDCS treatment. The possibility of cortical reorganization and stereoscopy recovery following brain stimulation points at tDCS as a useful strategy for treating amblyopia in adulthood. Furthermore, monocular deprivation in Long Evans rats is a valuable research model to study visual cortex mechanisms involved in depth perception and neural restoration after brain stimulation. metadata Castaño-Castaño, Sergio and Feijoo-Cuaresma, M. and Paredes-Pacheco, J. and Morales-Navas, M. and Ruiz-Guijarro, J.A. and Sanchez-Santed, F. and Nieto-Escámez, F. mail sergio.castano@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2019) tDCS recovers depth perception in adult amblyopic rats and reorganizes visual cortex activity. Behavioural Brain Research, 370. p. 111941. ISSN 01664328

2018

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Cerrado Inglés During the process of beeswax recycling, many industrial derivatives are obtained. These matrices may have an interesting healthy and commercial potential but to date they have not been properly studied. The aim of the present work was to evaluate the proximal and phytochemical composition, the antioxidant capacity and cytotoxic effects of two by-products from beeswax recycling process named MUD 1 and MUD 2 on liver hepatocellular carcinoma. Our results showed that MUD 1 presented the highest (P < .05) fiber, protein, carbohydrate, polyphenol and flavonoid concentration, as well as the highest (P < .05) total antioxidant capacity than the MUD 2 samples. MUD1 exerted also anticancer activity on HepG2 cells, by reducing cellular viability, increasing intracellular ROS levels and affecting mitochondrial functionality in a dose-dependent manner. We showed for the first time that by-products from beeswax recycling process can represent a rich source of phytochemicals with high total antioxidant capacity and anticancer activity; however, further researches are necessary to evaluate their potentiality for human health by in vivo studies. metadata Giampieri, Francesca and Quiles, José L. and Orantes-Bermejo, Francisco J. and Gasparrini, Massimiliano and Forbes-Hernandez, Tamara Y. and Sánchez-González, Cristina and Llopis, Juan and Rivas-García, Lorenzo and Afrin, Sadia and Varela-López, Alfonso and Cianciosi, Danila and Reboredo-Rodriguez, Patricia and Fernández-Piñar, Cristina Torres and Caderón Iglesia, Rubén and Ruiz Salces, Roberto and Aparicio Obregón, Silvia and Crespo-Álvarez, Jorge and Dzul Lopez, Luis and Xiao, Jianbo and Battino, Maurizio mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, ruben.calderon@uneatlantico.es, roberto.ruiz@uneatlantico.es, silvia.aparicio@uneatlantico.es, jorge.crespo@uneatlantico.es, luis.dzul@unini.edu.mx, UNSPECIFIED, maurizio.battino@uneatlantico.es (2018) Are by-products from beeswax recycling process a new promising source of bioactive compounds with biomedical properties? Food and Chemical Toxicology, 112. pp. 126-133. ISSN 0278-6915

Article Subjects > Biomedicine
Subjects > Nutrition
Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Cerrado Inglés Ample epidemiological evidence suggests a strong correlation among diet, lifestyle factors and the onset and consolidation of dementia and Alzheimer’s disease (AD). It has been demonstrated that AD, diabetes, obesity, insulin resistance, and cardiovascular disease are strongly interconnected pathologies. Preventive strategies and nutritional interventions seem to be promising approaches to delay neurocognitive decline and reduce the risk of AD and other non-psychiatric co-morbidities. In this regard, healthy dietary patterns, characterized by high intake of plant-based foods, probiotics, antioxidants, soy beans, nuts, and omega-3 polyunsaturated fatty acids, and a low intake of saturated fats, animal-derived proteins, and refined sugars, have been shown to decrease the risk of neurocognitive impairments and eventually the onset of AD. Here we review the role of some nutrients and, in particular, of healthy dietary patterns, such as the Mediterranean diet and other emerging healthy diets, DASH (Dietary Approach to Stop Hypertension) and MIND (Mediterranean-DASH dietIntervention for Neurodegenerative Delay), for the maintenance of cognitive performance, focusing specifically on human studies. The beneficial effects associated with overall diet composition, rather than single nutrient supplementations, for the prevention or the delay of AD and dementia are discussed. metadata Pistollato, Francesca and Calderón Iglesias, Rubén and Ruiz Salces, Roberto and Aparicio-Obregón, Silvia and Crespo-Álvarez, Jorge and Dzul Lopez, Luis Alonso and Manna, Piera Pia and Giampieri, Francesca and Battino, Maurizio mail francesca.pistollato@uneatlantico.es, ruben.calderon@uneatlantico.es, roberto.ruiz@uneatlantico.es, silvia.aparicio@uneatlantico.es, jorge.crespo@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2018) Nutritional patterns associated with the maintenance of neurocognitive functions and the risk of dementia and Alzheimer’s disease: A focus on human studies. Pharmacological Research, 131. pp. 32-43. ISSN 10436618

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production UNSPECIFIED UNSPECIFIED Berry fruits are rich in nutrients and polyphenols, providing potential health benefits. Understanding the factors that affect their bioavailability is becoming of utmost importance for evaluating their biological significance and efficacy as functional food. In this study, the phytochemical composition and the total antioxidant capacity of different varieties of five berries (blackberry, blackcurrant, blueberry, raspberry, and strawberry) were evaluated after an in vitro gastrointestinal digestion process. The cultivar of each berry that showed the higher content of total phenols and flavonoids was selected to study its cytotoxic effect on human hepatoma cells. Digestion resulted in a high reduction (p ˂ 0.05) of total phenolic, flavonoid and anthocyanin contents and total antioxidant capacity, in the “IN” samples compared to the “OUT” extracts, which represent the “serum-available” and the “colon-available” fractions, respectively. Incubation of the digested fraction for 24 h didn’t exert any effect on cellular viability, while a dose- and time-dependent cytotoxicity was observed after 48 h and 72 h of incubation for all the berries analyzed. Our results suggest that the approach proposed in this work may represent a rapid tool for evaluating and identifying new berries with increased phytochemical bioavailability, highlighting their antiproliferative agents after an in vitro digestion. metadata Giampieri, Francesca and Afrin, Sadia and Stewart, Derek and McDougall, Gordon and Brennan, Rex and Blyth, Lesley and Gasparrini, Massimiliano and Mazzoni, Luca and Capocasa, Franco and Alvarez-Suarez, José M. and Bompadre, Stefano and Nogueira Brás de Oliveira, Pedro and N. Santos, Claudia and Masias Vergara, Manuel and Agudo Toyos, Pablo and Crespo-Álvarez, Jorge and Mezzetti, Bruno and Forbes-Hernandez, Tamara Y. and Battino, Maurizio mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, manuel.masias@uneatlantico.es, pablo.agudo@uneatlantico.es, jorge.crespo@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2018) Phytochemical Composition and Cytotoxic Effects on Liver Hepatocellular Carcinoma Cells of Different Berries Following a Simulated In Vitro Gastrointestinal Digestion. Molecules, 23 (8). p. 1918. ISSN 1420-3049

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Cerrado Inglés A protracted pro-inflammatory state is the common denominator in the development, progression and complication of the common chronic diseases. Dietary antioxidants represent an efficient tool to counteract this inflammatory state. The aim of the present work was to evaluate the effects of strawberry extracts on inflammation evoked by E. Coli lipopolysaccharide in Human Dermal Fibroblast, by measuring reactive oxygen species production, apoptosis rate, antioxidant enzymes activity, mitochondria functionality and also investigating the molecular pathway involved in inflammatory and antioxidant response. The results demonstrated that strawberry pre-treatment reduced intracellular reactive oxygen species levels, apoptotic rate, improved antioxidant defences and mitochondria functionality in lipopolysaccharide -treated cells. Strawberry exerted these protective activities through the inhibition of the NF-kB signalling pathway and the stimulation of the Nrf2 pathway, with a mechanism AMPK-dependent. These results confirm the health benefits of strawberry in the prevention of inflammation and oxidative stress condition in lipopolysaccharide-treated cells. metadata Gasparrini, Massimiliano and Giampieri, Francesca and Forbes-Hernandez, Tamara Y. and Afrin, Sadia and Cianciosi, Danila and Reboredo-Rodriguez, Patricia and Varela-Lopez, Alfonso and Zhang, JiaoJiao and Quiles, Josè L. and Mezzetti, Bruno and Bompadre, Stefano and Battino, Maurizio mail UNSPECIFIED, UNSPECIFIED, tamara.forbes@unini.edu.mx, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, maurizio.battino@uneatlantico.es (2018) Strawberry extracts efficiently counteract inflammatory stress induced by the endotoxin lipopolysaccharide in Human Dermal Fibroblast. Food and Chemical Toxicology, 114. pp. 128-140. ISSN 0278-6915

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Cerrado Español Introducción La disnea es un síntoma con un componente multidimensional, aunque las herramientas que se utilizan habitualmente para evaluarla no tienen en cuenta esta faceta. El cuestionario Disnea-12 valora la multidimensionalidad de la disnea, específicamente las dimensiones afectiva y sensorial. El objetivo de este estudio es validar el cuestionario Disnea-12 al español. Métodos Se realizó una traducción del original en inglés al español y del español al inglés para verificar la equivalencia del texto. Posteriormente se verificó la comprensión del texto tras pasárselo a 10 pacientes. La fiabilidad y la validez del cuestionario se estudiaron en un grupo independiente de EPOC diagnosticados y clasificados por las guías GOLD de las consultas externas de neumología del Hospital Universitario Marqués de Valdecilla. Resultados El grupo (n = 51) tenía una media de edad de 65 años y un FEV1 medio del 50%. Todos los pacientes entendieron las preguntas del cuestionario. El instrumento presentó consistencia interna de α = 0,937 y un coeficiente de correlación intraclase: 0,969; p < 0,001. Se encontraron correlaciones estadísticamente significativas con las puntuaciones del HAD (HADansiedad r = 0,608 y HADdepresión r = 0,615), disnea de la mMRC (r = 0,592), T6MM (r = –0,445), FEV1 (r = –0,312), las 4 dimensiones de CRQ-SAS (disnea r = –0,626; fatiga r = –0,718; función emocional r = –0,663; control de enfermedad r = –0,740), el CAT (r = 0,669) y el índice de disnea basal (r = –0,615). Los grupos GOLD más sintomáticos (B y D) presentaron una puntuación 10,32 puntos mayor en en el Disnea-12 (p < 0,001). Conclusión El cuestionario Disnea-12 es un instrumento válido y fiable para evaluar la disnea de forma multidimensional. metadata Amado Diago, Carlos Antonio and Puente Maestu, Luis and Abascal Bolado, Beatriz and Agüero Calvo, Juan and Hernando Hernando, Mercedes and Puente Bats, Irene and Agüero Balbín, Ramón mail UNSPECIFIED (2018) Traducción y validación del cuestionario multidimensional Disnea-12. Archivos de Bronconeumología, 54 (2). pp. 74-78. ISSN 03002896

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Cerrado Inglés Introduction Dyspnea is a multidimensional symptom, but this multidimensionality is not considered in most dyspnea questionnaires. The Dyspnea-12 takes a multidimensional approach to the assessment of dyspnea, specifically the sensory and the affective response. The objective of this study was to translate into Spanish and validate the Dyspnea-12 questionnaire. Methods The original English version of the Dyspnea-12 questionnaire was translated into Spanish and backtranslated to analyze its equivalence. Comprehension of the text was verified by analyzing the responses of 10 patients. Reliability and validation of the questionnaire were studied in an independent group of COPD patients attending the pulmonology clinics of Hospital Universitario Marqués de Valdecilla, diagnosed and categorized according to GOLD guidelines. Results The mean age of the group (n=51) was 65 years and mean FEV1 was 50%. All patients understood all questions of the translated version of Dyspnea-12. Internal consistency of the questionnaire was α=0.937 and intraclass correlation coefficient was =0.969; P<.001. Statistically significant correlations were found with HADS (anxiety r=0.608 and depression r=0.615), mMRC dyspnea (r=0.592), 6MWT (r=–0.445), FEV1 (r=–0.312), all dimensions of CRQ-SAS (dyspnea r=–0.626; fatigue r=–0.718; emotional function r=–0.663; mastery r=–0.740), CAT (r=0.669), and baseline dyspnea index (r=–0.615). Dyspnea-12 scores were 10.32 points higher in symptomatic GOLD groups (B and D) (P<.001). Conclusion The Spanish version of Dyspnea-12 is a valid and reliable instrument to study the multidimensional nature of dyspnea. metadata Amado Diago, Carlos Antonio and Puente Maestu, Luis and Abascal Bolado, Beatriz and Agüero Calvo, Juan and Hernando Hernando, Mercedes and Puente Bats, Irene and Agüero Balbín, Ramón mail UNSPECIFIED (2018) Translation and Validation of the Multidimensional Dyspnea-12 Questionnaire. Archivos de Bronconeumología (English Edition), 54 (2). pp. 74-78. ISSN 15792129

2017

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Cerrado Inglés A common denominator in the pathogenesis of most chronic inflammatory diseases is the involvement of oxidative stress, related to ROS production by all aerobic organisms. Dietary antioxidants from plant foods represent an efficient strategy to counteract this condition. The aim of the present study was to evaluate the protective effects of strawberry extracts on inflammatory status induced by E. Coli LPS on RAW 264.7 macrophages by measuring the main oxidative and inflammatory biomarkers and investigating the molecular pathways involved. Strawberry pre-treatment efficiently counteracted LPS-induced oxidative stress reducing the amount of ROS and nitrite production, stimulating endogenous antioxidant enzyme activities and enhancing protection against lipid, protein and DNA damage (P < 0.05). Strawberry pre-treatment exerted these protective effects primarily through the activation of the Nrf2 pathway, which is markedly AMPK-dependent and also by the modulation of the NF-kB signalling pathway. Finally, an improvement in mitochondria functionality was also detected. The results obtained in this work highlight the health benefit of strawberries against inflammatory and oxidative stress in LPS-stimulated RAW 264.7 macrophages, investigating for the first time the possible involved molecular mechanisms. metadata Gasparrini, Massimiliano and Forbes-Hernandez, Tamara Y. and Giampieri, Francesca and Afrin, Sadia and Alvarez-Suarez, Josè M. and Mazzoni, Luca and Mezzetti, Bruno and Quiles, Josè L. and Battino, Maurizio mail UNSPECIFIED, tamara.forbes@unini.edu.mx, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, maurizio.battino@uneatlantico.es (2017) Anti-inflammatory effect of strawberry extract against LPS-induced stress in RAW 264.7 macrophages. Food and Chemical Toxicology, 102. pp. 1-10. ISSN 0278-6915

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Cerrado Inglés Conventional cardiovascular risk factors (CVRFs) are accepted to identify asymptomatic individuals with high risk of acute myocardial infarction (AMI). However, AMI affects many patients previously classified at low risk. New biomarkers are needed to improve risk prediction. We propose to evaluate the NLRP3-inflammasome complex as a potential conventional cardiovascular risk (CVR) indicator in healthy males and post-AMI patients and compare both groups by known CVRFs. We included 109 men with no history of cardiovascular disease (controls) and 150 AMI patients attending a cardiac rehabilitation program. AMI patients had higher mean of body mass index (BMI) and waist circumference than the controls. However, high percentages of the controls had a high BMI and a waist circumference >95 cm. The controls also had higher systolic blood pressure (p > 0.001), total and low-density lipoprotein cholesterol, dietary nutrient, and calorific intake. Fuster BEWAT score (FBS) correlated more closely than Framingham risk score (FRS) with most CVRF, groups. However, only the FBS showed a correlation with inflammasome cytokine interleukin 1β (IL-1β). Several CVRFs were significantly better in AMI patients; however, this group also had higher mRNA expression of the inflammasome gene NLRP3 and lower expression of the autophagy gene MAP-LC3. The controls had high levels of CVRF, probably reflecting unhealthy lifestyle. FBS reflects the efficiency of strategies to induce lifestyle changes such as cardiac rehabilitation programs, and could provide a sensitive evaluation CVR. These results lead to the hypothesis that NLRP3-inflammasome and associated IL-1β release have potential as CVR biomarkers, particularly in post-AMI patients with otherwise low risk scores. Antioxid. Redox Signal. 27, 269-275. metadata Bullón, Pedro and Cano-García, Francisco J. and Alcocer-Gómez, Elísabet and Varela-López, Alfonso and Roman-Malo, Lourdes and Ruiz-Salmerón, Rafael J. and Quiles, José L. and Navarro-Pando, José M. and Battino, Maurizio and Ruiz-Cabello, Jesús and Jiménez-Borreguero, Luis J. and Cordero, Mario D. mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, maurizio.battino@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2017) Could NLRP3–Inflammasome Be a Cardiovascular Risk Biomarker in Acute Myocardial Infarction Patients? Antioxidants & Redox Signaling, 27 (5). pp. 269-275. ISSN 1523-0864

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
UNSPECIFIED UNSPECIFIED Regulation of lipid metabolism is essential for treatment and prevention of several chronic diseases such as obesity, diabetes, and cardiovascular diseases, which are responsible for most deaths worldwide. It has been demonstrated that the AMP-activated protein kinase (AMPK) has a direct impact on lipid metabolism by modulating several downstream-signaling components. The main objective of the present work was to evaluate the in vitro effect of a methanolic strawberry extract on AMPK and its possible repercussion on lipid metabolism in human hepatocellular carcinoma cells (HepG2). For such purpose, the lipid profile and the expression of proteins metabolically related to AMPK were determined on cells lysates. The results demonstrated that strawberry methanolic extract decreased total cholesterol, low-density lipoprotein (LDL)-cholesterol, and triglycerides levels (up to 0.50-, 0.30-, and 0.40-fold, respectively) while it stimulated the p-AMPK/AMPK expression (up to 3.06-fold), compared to the control. AMPK stimulation led to the phosphorylation and consequent inactivation of acetyl coenzyme A carboxylase (ACC) and inhibition of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), the major regulators of fatty acids and cholesterol synthesis, respectively. Strawberry treatment also entailed a 4.34-, 2.37-, and 2.47-fold overexpression of LDL receptor, sirtuin 1 (Sirt1), and the peroxisome proliferator activated receptor gamma coactivator 1-alpha (PGC-1α), respectively, compared to control. The observed results were counteracted by treatment with compound C, an AMPK pharmacological inhibitor, confirming that multiple effects of strawberries on lipid metabolism are mediated by the activation of this protein. metadata Forbes-Hernandez, Tamara Y. and Giampieri, Francesca and Gasparrini, Massimiliano and Afrin, Sadia and Mazzoni, Luca and Cordero, Mario and Mezzetti, Bruno and Quiles, José L. and Battino, Maurizio mail tamara.forbes@unini.edu.mx, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, maurizio.battino@uneatlantico.es (2017) Lipid Accumulation in HepG2 Cells Is Attenuated by Strawberry Extract through AMPK Activation. Nutrients, 9 (6). p. 621. ISSN 2072-6643

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
UNSPECIFIED UNSPECIFIED A protracted pro-inflammatory state is a major contributing factor in the development, progression and complication of the most common chronic pathologies. Fruit and vegetables represent the main sources of dietary antioxidants and their consumption can be considered an efficient tool to counteract inflammatory states. In this context an evaluation of the protective effects of strawberry extracts on inflammatory stress induced by E. coli LPS on human dermal fibroblast cells was performed in terms of viability assays, ROS and nitrite production and biomarkers of oxidative damage of the main biological macromolecules. The results demonstrated that strawberry extracts exerted an anti-inflammatory effect on LPS-treated cells, through an increase in cell viability, and the reduction of ROS and nitrite levels, and lipid, protein and DNA damage. This work showed for the first time the potential health benefits of strawberry extract against inflammatory and oxidative stress in LPS-treated human dermal fibroblast cells. metadata Gasparrini, Massimiliano and Forbes-Hernandez, Tamara Y. and Giampieri, Francesca and Afrin, Sadia and Mezzetti, Bruno and Quiles, José L. and Bompadre, Stefano and Battino, Maurizio mail UNSPECIFIED, tamara.forbes@unini.edu.mx, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, maurizio.battino@uneatlantico.es (2017) Protective Effect of Strawberry Extract against Inflammatory Stress Induced in Human Dermal Fibroblasts. Molecules, 22 (1). p. 164. ISSN 1420-3049

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
UNSPECIFIED UNSPECIFIED Dyslipidemia and oxidation of low density lipoproteins (LDL) are recognized as critical factors in the development of atherosclerosis. Healthy dietary patterns, with abundant fruit and vegetable consumption, may prevent the onset of these risk factors due to the presence of phytochemical compounds. Strawberries are known for their high content of polyphenols; among them, flavonoids are the major constituents, and it is presumed that they are responsible for the biological activity of the fruit. Nevertheless, there are only a few studies that actually evaluate the effects of different fractions isolated from strawberries. In order to assess the effects of two different strawberry extracts (whole methanolic extract/anthocyanin-enriched fraction) on the lipid profile and antioxidant status in human hepatocellular carcinoma (HepG2) cells, the triglycerides and LDL-cholesterol content, lipid peroxidation, intracellular reactive oxygen species (ROS) content and antioxidant enzymes’ activity on cell lysates were determined. Results demonstrated that both strawberry extracts not only improved the lipid metabolism by decreasing triglycerides and LDL-cholesterol contents, but also improved the redox state of HepG2 cells by modulating thiobarbituric acid-reactive substances production, antioxidant enzyme activity and ROS generation. The observed effects were more pronounced for the anthocyanin-enriched fraction. metadata Forbes-Hernandez, Tamara Y. and Gasparrini, Massimiliano and Afrin, Sadia and Cianciosi, Danila and González-Paramás, Ana and Santos-Buelga, Celestino and Mezzetti, Bruno and Quiles, José L. and Battino, Maurizio and Giampieri, Francesca and Bompadre, Stefano mail tamara.forbes@unini.edu.mx, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, maurizio.battino@uneatlantico.es, UNSPECIFIED, UNSPECIFIED (2017) Strawberry (cv. Romina) Methanolic Extract and Anthocyanin-Enriched Fraction Improve Lipid Profile and Antioxidant Status in HepG2 Cells. International Journal of Molecular Sciences, 18 (6). p. 1149. ISSN 1422-0067

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
UNSPECIFIED UNSPECIFIED Extreme exposure of skin to Ultraviolet A (UVA)-radiation may induce a dysregulated production of reactive oxygen species (ROS) which can interact with cellular biomolecules leading to oxidative stress, inflammation, DNA damage, and alteration of cellular molecular pathways, responsible for skin photoaging, hyperplasia, erythema, and cancer. For these reasons, the use of dietary natural bioactive compounds with remarkable antioxidant activity could be a strategic tool to counteract these UVA-radiation-caused deleterious effects. Thus, the purpose of the present work was to test the efficacy of strawberry (50 μg/mL)-based formulations supplemented with Coenzyme Q10 (100 μg/mL) and sun protection factor 10 in human dermal fibroblasts irradiated with UVA-radiation. The apoptosis rate, the amount of intracellular reactive oxygen species (ROS) production, the expression of proteins involved in antioxidant and inflammatory response, and mitochondrial functionality were evaluated. The results showed that the synergic topical use of strawberry and Coenzyme Q10 provided a significant (p < 0.05) photoprotective effect, reducing cell death and ROS, increasing antioxidant defense, lowering inflammatory markers, and improving mitochondrial functionality. The obtained results suggest the use of strawberry-based formulations as an innovative, natural, and useful tool for the prevention of UVA exposure-induced skin diseases in order to decrease or substitute the amount of synthetic sunscreen agents. metadata Gasparrini, Massimiliano and Forbes-Hernandez, Tamara Y. and Afrin, Sadia and Reboredo-Rodriguez, Patricia and Cianciosi, Danila and Mezzetti, Bruno and Quiles, José L. and Bompadre, Stefano and Battino, Maurizio and Giampieri, Francesca mail UNSPECIFIED, tamara.forbes@unini.edu.mx, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, maurizio.battino@uneatlantico.es, UNSPECIFIED (2017) Strawberry-Based Cosmetic Formulations Protect Human Dermal Fibroblasts against UVA-Induced Damage. Nutrients, 9 (6). p. 605. ISSN 2072-6643

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production UNSPECIFIED UNSPECIFIED Honey is a natural product known to modulate several biological activities including cancer. The aim of the present study was to examine the phytochemical content and the antioxidant activity of Strawberry tree (Arbutus unedo) honey (STH) and its cytotoxic properties against human colon adenocarcinoma (HCT-116) and metastatic (LoVo) cell lines in comparison with Manuka (Leptospermum scoparium) honey (MH). Several unifloral STH and MH were analyzed for their phenolic, flavonoid, amino acid and protein contents, as well as their radical scavenging activities. STH from the Berchidda area showed the highest amount of phenolic, flavonoid, amino acid and protein content, and antioxidant capacity compared to MH. Both STH and MH induced cytotoxicity and cell death in a dose- and time-dependent manner in HCT-116 and LoVo cells, with less toxicity on non-cancer cells. Compared to MH, STH showed more effect at lower concentrations on HCT-116 and LoVo cells. In addition, both honeys increased intracellular reactive oxygen species (ROS) generation. In HCT-116 cells, STH and MH induced similar ROS production but in LoVo cells STH induced a higher percentage of ROS compared to MH. Our results indicate that STH and MH can induce cell growth inhibition and ROS generation in colon adenocarcinoma and metastatic cells, which could be due to the presence of phytochemicals with antioxidant properties. These preliminary results are interesting and suggest a potential chemopreventive action which could be useful for further studies in order to develop chemopreventive agents for colon cancer. metadata Afrin, Sadia and Forbes-Hernandez, Tamara Y. and Gasparrini, Massimiliano and Bompadre, Stefano and Quiles, José and Sanna, Gavino and Spano, Nadia and Giampieri, Francesca and Battino, Maurizio mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, maurizio.battino@uneatlantico.es (2017) Strawberry-Tree Honey Induces Growth Inhibition of Human Colon Cancer Cells and Increases ROS Generation: A Comparison with Manuka Honey. International Journal of Molecular Sciences, 18 (3). p. 613. ISSN 1422-0067

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Cerrado Español Introducción Las recomendaciones sobre la reanimación cardiopulmonar (RCP) enfatizan la calidad de las maniobras, en especial las compresiones torácicas (CT). Los dispositivos de retroalimentación podrían mejorar la calidad de las CT durante la RCP. El objetivo de este estudio fue evaluar la utilidad de un dispositivo lumínico simple como ayuda visual durante la RCP en un maniquí. Material y métodos Veintidós mujeres pediatras que asistieron a un curso acreditado de RCP pediátrica realizaron, siguiendo un orden aleatorio, 2 min de RCP en un maniquí sin y con la ayuda de un dispositivo luminoso simple, que emite destellos con una frecuencia de 100 ciclos por minuto. Se analizaron mediante un medidor validado de la calidad de las compresiones (CPRmeter®) las siguientes variables de las CT: profundidad, descompresiones, frecuencia, tiempo de RCP y porcentaje de compresiones. Resultados Con el dispositivo luminoso, las participantes aumentaron la calidad media (60,23 ± 54,50 vs. 79,24 ± 9,80%; p = 0,005), la profundidad (48,86 ± 42,67 vs. 72,95 ± 20,25%; p = 0,036) y la frecuencia del masaje (35,82 ± 37,54 vs. 67,09 ± 31,95%; p = 0,024). Conclusiones Un dispositivo luminoso simple que parpadea a la frecuencia recomendada mejora la calidad de las CT realizadas por residentes de pediatría en un maniquí. Se debería evaluar la utilidad de este sistema de ayuda de la RCP en pacientes reales. metadata González-Calvete, L. and Barcala-Furelos, R. and Moure-González, J.D. and Abelairas-Gómez, Cristian and Rodríguez-Núñez, A. mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, cristian.abelairas@uneatlantico.es, UNSPECIFIED (2017) Utilidad de un dispositivo luminoso simple para mejorar el aprendizaje del masaje cardiaco. Revista Española de Anestesiología y Reanimación, 64 (9). pp. 506-512. ISSN 00349356

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Cerrado Inglés It is generally accepted that a fruit and vegetable–enriched diet is favorable for human health. The consumption of strawberries, in particular, has been related to the maintenance of well-being and the prevention of several chronic diseases, owing to the high contents of antioxidants and phytochemicals present in the fruit. Several biological effects have been explained through the total antioxidant capacity exerted by these bioactive compounds, but recently more intricate mechanisms have begun to be examined. In this context, it has been reported that strawberry phenolics are able to exert anti-inflammatory, anticarcinogenic, antiproliferative, and antiatherosclerotic activities, acting on specific molecular pathways related to antioxidant defenses, metabolism, survival, and proliferation. The overall aim of this work is to discuss and update the cellular and molecular mechanisms recently proposed to clarify the effects of strawberry phenolics on human health, with particular attention to the most common chronic diseases, such as metabolic syndrome, cardiovascular disease, and cancer. metadata Giampieri, Francesca and Forbes-Hernandez, Tamara Y. and Gasparrini, Massimiliano and Afrin, Sadia and Cianciosi, Danila and Reboredo-Rodriguez, Patricia and Varela-Lopez, Alfonso and Quiles, Jose L. and Mezzetti, Bruno and Battino, Maurizio mail UNSPECIFIED, tamara.forbes@unini.edu.mx, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, maurizio.battino@uneatlantico.es (2017) The healthy effects of strawberry bioactive compounds on molecular pathways related to chronic diseases. Annals of the New York Academy of Sciences, 1398 (1). pp. 62-71. ISSN 0077-8923

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Cerrado Inglés Acerola fruits (Malpighia emarginata DC.) from the central region of Cuba were analyzed to determine their chemical composition and protective capacity against oxidative damage using an in vitro human dermal fibroblast (HDFa) model. The chemical composition analyses showed a high content of vitamin C, total polyphenols, β-carotene and folates in the acerola fruit. From the HPLC-DAD/ESI-MSn analyses, two anthocyanins (cyanidin 3-O-rhamnoside and pelargonidin 3-O-rhamnoside), three hydroxycinnamoyl derivatives (caffeoyl hexoside, dihydrocaffeoylquinic acid and coumaroyl hexoside) and fifteen flavonols (mostly glycosylated forms of quercetin and kaempferol) were detected. HDFa were pre-incubated with an acerola crude extract (ACExt) and subsequently subjected to oxidative stress induced by AAPH. Apoptosis, intracellular ROS and the biomarkers of lipid and protein oxidation significantly increased after inducing stress, while the activities of the antioxidant enzyme catalase and superoxide dismutase and mitochondrial functionality were markedly affected. However, ACExt was able to protect against oxidative damage through decreasing apoptosis, intracellular ROS levels and lipid and protein damage, besides improving antioxidant enzyme activities and mitochondrial functionality. The obtained results support acerola fruits as relevant sources of functional compounds with promising effects on human health. metadata Alvarez-Suarez, José M. and Giampieri, Francesca and Gasparrini, Massimiliano and Mazzoni, Luca and Santos-Buelga, Celestino and González-Paramás, Ana M. and Forbes-Hernández, Tamara Y. and Afrin, Sadia and Páez-Watson, Timothy and Quiles, José L. and Battino, Maurizio mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, tamara.forbes@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, maurizio.battino@uneatlantico.es (2017) The protective effect of acerola (Malpighia emarginata) against oxidative damage in human dermal fibroblasts through the improvement of antioxidant enzyme activity and mitochondrial functionality. Food & Function, 8 (9). pp. 3250-3258. ISSN 2042-6496

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Cerrado Inglés Among gynaecological cancers, ovarian cancer represents the leading cause of death in women. Current treatment for ovarian cancer entails surgery followed by combined chemotherapy with platinum and taxane, which are associated, particularly cisplatin, with severe side effects. While this treatment approach appears to be initially effective in a high number of patients, nearly 70% of them suffer a relapse within a few months after initial treatment. Therefore, more effective and better-tolerated treatment options are clearly needed. In recent years, several natural compounds (such as curcumin, epigallocatechin 3-gallate (EGCG), resveratrol, sulforaphane and Withaferin-A), characterized by long-term safety and negligible and/or inexistent side effects, have been proposed as possible adjuvants of traditional chemotherapy. Indeed, several in vitro and in vivo studies have shown that phytocompounds can effectively inhibit tumor cell proliferation, stimulate autophagy, induce apoptosis, and specifically target ovarian cancer stem cells (CSCs), which are generally considered to be responsible for tumor recurrence in several types of cancer. Here we review current literature on the role of natural products in ovarian cancer chemoprevention, highlighting their effects particularly on the regulation of inflammation, autophagy, proliferation and apoptosis, chemotherapy resistance, and ovarian CSC growth. metadata Pistollato, Francesca and Calderón Iglesias, Rubén and Ruiz Salces, Roberto and Aparicio Obregón, Silvia and Crespo Alvare, Jorge and Dzul Lopez, Luis and Giampieri, Francesca and Battino, Maurizio mail UNSPECIFIED, ruben.calderon@uneatlantico.es, roberto.ruiz@uneatlantico.es, silvia.aparicio@uneatlantico.es, jorge.crespo@uneatlantico.es, luis.dzul@unini.edu.mx, UNSPECIFIED, maurizio.battino@uneatlantico.es (2017) The use of natural compounds for the targeting and chemoprevention of ovarian cancer. Cancer Letters, 411. pp. 191-200. ISSN 0304-3835

2016

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Cerrado Inglés Excessive amounts of free radicals are deleterious for cells, resulting in cell damage, affecting the wound healing process and causing premature ageing or even neoplastic transformation. Here the capacity of Manuka honey (MH) to protect against oxidative damage and improve the process of skin wound healing was investigated. Up to 16 compounds were identified in MH, with leptosin derivatives and methyl syringate as the major ones. MH protected against apoptosis, intracellular ROS production, and lipid and protein oxidative damage. MH also protected mitochondrial functionality, promoted cell proliferation and activated the AMPK/Nrf2/ARE signalling pathway, as well as the expression of the antioxidant enzymes such as SOD and CAT. Here we describe for the first time that one of the possible mechanisms by which MH exhibits its ability to promote wound healing could be due to its capacity to improve the antioxidant response by activating AMPK phosphorylation and the ARE response. metadata Alvarez-Suarez, José Miguel and Giampieri, Francesca and Cordero, Mario and Gasparrini, Massimiliano and Forbes-Hernandez, Tamara Yuliet and Mazzoni, Luca and Afrin, Sadia and Beltrán-Ayala, Pablo and González-Paramás, Ana María and Santos-Buelga, Celestino and Varela-Lopez, Alfonso and Quiles, José Luis and Battino, Maurizio mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, tamara.forbes@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, maurizio.battino@uneatlantico.es (2016) Activation of AMPK/Nrf2 signalling by Manuka honey protects human dermal fibroblasts against oxidative damage by improving antioxidant response and mitochondrial function promoting wound healing. Journal of Functional Foods, 25. pp. 38-49. ISSN 1756-4646

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Abierto Inglés Papillon–Lefèvre syndrome (PLS) (OMIM: 245000) is a rare disease characterized by severe periodontitis and palmoplantar keratoderma. It is caused by mutations in both alleles of the cathepsin C (CatC) gene CTSC that completely abrogate the proteolytic activity of this cysteine proteinase. Most often, a genetic analysis to enable early and rapid diagnosis of PLS is unaffordable or unavailable. In this study, we tested the hypothesis that active CatC is constitutively excreted and can be easily traced in the urine of normal subjects. If this is true, determining its absence in the urine of patients would be an early, simple, reliable, low-cost and easy diagnostic technique. All 75 urine samples from healthy control subjects (aged 3 months to 80 years) contained proteolytically active CatC and its proform, as revealed by kinetic analysis and immunochemical detection. Of the urine samples of 31 patients with a PLS phenotype, 29 contained neither proteolytically active CatC nor the CatC antigen, so that the PLS diagnosis was confirmed. CatC was detected in the urine of the other two patients, and genetic analysis revealed no loss-of-function mutation in CTSC, indicating that they suffer from a PLS-like condition but not from PLS. Screening for the absence of urinary CatC activity soon after birth and early treatment before the onset of PLS manifestations will help to prevent aggressive periodontitis and loss of many teeth, and should considerably improve the quality of life of PLS patients. metadata Hamon, Yveline and Legowska, Monika and Fergelot, Patricia and Dallet-Choisy, Sandrine and Newell, Louise and Vanderlynden, Lise and Kord Valeshabad, Ali and Acrich, Karina and Kord, Hadi and Tsamakis, Charalampos and Morice-Picard, Fanny and Surplice, Ian and Zoidakis, Jerome and David, Karen and Vlahou, Antonia and Ragunatha, Shivanna and Nagy, Nikoletta and Farkas, Katalin and Széll, Márta and Goizet, Cyril and Schacher, Beate and Battino, Maurizio and Al Farraj Aldosari, Abdullah and Wang, Xinwen and Liu, Yang and Marchand-Adam, Sylvain and Lesner, Adam and Kara, Elodie and Korkmaz-Icöz, Sevil and Moss, Celia and Eickholz, Peter and Taieb, Alain and Kavukcu, Salih and Jenne, Dieter E. and Gauthier, Francis and Korkmaz, Brice mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, maurizio.battino@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED (2016) Analysis of urinary cathepsin C for diagnosing Papillon-Lefèvre syndrome. FEBS Journal, 283 (3). pp. 498-509. ISSN 1742464X

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production UNSPECIFIED UNSPECIFIED Colon cancer is one of the most prevalent diseases across the world. Numerous epidemiological studies indicate that diets rich in fruit, such as berries, provide significant health benefits against several types of cancer, including colon cancer. The anticancer activities of berries are attributed to their high content of phytochemicals and to their relevant antioxidant properties. In vitro and in vivo studies have demonstrated that berries and their bioactive components exert therapeutic and preventive effects against colon cancer by the suppression of inflammation, oxidative stress, proliferation and angiogenesis, through the modulation of multiple signaling pathways such as NF-κB, Wnt/β-catenin, PI3K/AKT/PKB/mTOR, and ERK/MAPK. Based on the exciting outcomes of preclinical studies, a few berries have advanced to the clinical phase. A limited number of human studies have shown that consumption of berries can prevent colorectal cancer, especially in patients at high risk (familial adenopolyposis or aberrant crypt foci, and inflammatory bowel diseases). In this review, we aim to highlight the findings of berries and their bioactive compounds in colon cancer from in vitro and in vivo studies, both on animals and humans. Thus, this review could be a useful step towards the next phase of berry research in colon cancer. metadata Afrin, Sadia and Giampieri, Francesca and Gasparrini, Massimiliano and Forbes-Hernandez, Tamara and Varela-López, Alfonso and Quiles, José and Mezzetti, Bruno and Battino, Maurizio mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, maurizio.battino@uneatlantico.es (2016) Chemopreventive and Therapeutic Effects of Edible Berries: A Focus on Colon Cancer Prevention and Treatment. Molecules, 21 (2). p. 169. ISSN 1420-3049

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production UNSPECIFIED UNSPECIFIED Coenzyme Q (CoQ) is a naturally occurring molecule located in the hydrophobic domain of the phospholipid bilayer of all biological membranes. Shortly after being discovered, it was recognized as an essential electron transport chain component in mitochondria where it is particularly abundant. Since then, more additional roles in cell physiology have been reported, including antioxidant, signaling, death prevention, and others. It is known that all cells are able to synthesize functionally sufficient amounts of CoQ under normal physiological conditions. However, CoQ is a molecule found in different dietary sources, which can be taken up and incorporated into biological membranes. It is known that mitochondria have a close relationship with the aging process. Additionally, delaying the aging process through diet has aroused the interest of scientists for many years. These observations have stimulated investigation of the anti-aging potential of CoQ and its possible use in dietary therapies to alleviate the effects of aging. In this context, the present review focus on the current knowledge and evidence the roles of CoQ cells, its relationship with aging, and possible implications of dietary CoQ in relation to aging, lifespan or age-related diseases. metadata Varela-López, Alfonso and Giampieri, Francesca and Battino, Maurizio and Quiles, Jose L. mail UNSPECIFIED, UNSPECIFIED, maurizio.battino@uneatlantico.es, UNSPECIFIED (2016) Coenzyme Q and Its Role in the Dietary Therapy against Aging. Molecules, 21 (3). p. 373. ISSN 1420-3049

Other Subjects > Biomedicine Europe University of Atlantic > Research > Projects I+D+I Cerrado Español En el mundo, algunas de las principales enfermedades no transmisibles se producen principalmente por hipertensión arterial, sobrepeso/obesidad e hiperglucemia. El proyecto pretende desarrollar la tecnología digital necesaria para mejorar sensiblemente el seguimiento de los tratamientos dietéticos analizando, comparando y clasificando perfiles de individuos obtenidos a partir de datos digitales correspondientes a personas que incluyen rasgos de la personalidad e informaciones que evolucionan en el tiempo. La tecnología clave a desarrollar debe permitir obtener perfiles característicos de los individuos (pacientes), analizarlos comparativamente y visualmente, y clasificarlos con objeto a poder lanzar actuaciones personalizadas, principalmente por mensajería, y realizar una evaluación posterior sobre la eficacia de las actuaciones promovidas por el sistema hacia dichos individuos. Estratégicamente, CITICAN pretende contar con tecnologías propias de análisis de perfiles de individuos que tienen aplicación potencial en otros campos como la formación online o el desarrollo de servicios digitales personalizados. Objetivo del Proyecto: Desarrollar la tecnología digital necesaria para mejorar sensiblemente el seguimiento de los tratamientos dietéticos analizando, comparando y clasificando perfiles de individuos obtenidos a partir de datos digitales correspondientes a personas que incluyen rasgos de la personalidad e informaciones que evolucionan en el tiempo. Financiación Este proyecto ha sido cofinanciado por la Sociedad de Desarrollo Regional de Cantabria (SODERCAN) y el el Programa Operativo FEDER de Cantabria en el marco del programa denominado I+C= +C 2016 (Investigación + Conocimiento= +Cantabria) que tiene por objetivo el fortalecimiento del tejido industrial de la región. Inicio: 01/09/2016 Fin: 31/08/2018 Código Externo: BI16-IN-005 metadata Centro de Investigación y Tecnología Industrial de Cantabria, CITICAN mail citican@devnull.uneatlantico.es (2016) NUTRIX: Desarrollo de tecnologías para el análisis de pacientes en tratamientos dietéticos mediante sistemas expertos y redes neuronales. Repositorio de la Universidad. (Unpublished)

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Cerrado Inglés This chapter describes the significance of oxidative stress and its involvement in major oral and general diseases, and discusses damage inflicted by oxidative stress to cellular structures. Atmospheric oxygen presents two unpaired electrons in the outer electron shell. Oxygen reduction takes place in a sequential addition of electrons that leads to reactive oxygen species (ROS) formation among which are superoxide, hydrogen peroxide, and hydroxyl radical. There is solid evidence that many oral diseases such as periodontitis or oral cancer are directly linked to loss of balance between antioxidant systems and endogenous or exogenous prooxidants. Moreover, oxidative stress is directly associated with several general pathological conditions, and in many cases, it represents the systemic link between the initiation of general diseases and the development of various oral conditions. These observations thoroughly justify the continuous search for new antioxidants as well as antioxidant usage in the prophylaxis and treatment of oral diseases. metadata Battino, Maurizio and Greabu, Maria and Calenic, Bogdan mail UNSPECIFIED (2016) Oxidative stress in oral cavity: interplay between reactive oxygen species and antioxidants in health, inflammation, and cancer. Wiley Online Library. pp. 155-166.

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Cerrado Inglés Doxorubicin (Dox), one of the most used chemotherapeutic agents, is known to generate oxidative stress and block DNA synthesis, which result in severe dose-limiting toxicity. A strategy to protect against Dox toxic effects could be to use dietary antioxidants of which fruits and vegetable are a rich source. In this context, strawberry consumption is associated with the maintenance of good health and the prevention of several diseases, thanks to the antioxidant capacities of its bioactive compounds. The aim of the present study was to evaluate the protective effects of strawberry consumption against oxidative stress induced by Dox in rats. Animals were fed with strawberry enriched diet (15% of the total calories) for two months and Dox (10 mg/kg; i.p.) was injected at the end of the experimental period. Strawberry consumption significantly inhibited ROS production and oxidative damage biomarkers accumulation in plasma and liver tissue and alleviated histopathological changes in rat livers treated with Dox. The reduction of antioxidant enzyme activities was significantly mitigated after strawberry consumption. In addition, strawberry enriched diet ameliorated liver mitochondrial antioxidant levels and functionality. In conclusion, strawberry intake protects against Dox-induced toxicity, at plasma, liver and mitochondrial levels thanks to its high contents of bioactive compounds. metadata Giampieri, Francesca and Alvarez-Suarez, Jose M. and Gasparrini, Massimiliano and Forbes- Hernandez, Tamara Y. and Afrin, Sadia and Bompadre, Stefano and Rubini, Corrado and Zizzi, Antonio and Astolfi, Paola and Santos-Buelga, Celestino and González-Paramás, Ana M. and Quiles, Josè L. and Mezzetti, Bruno and Battino, Maurizio mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, tamara.forbes@unini.edu.mx, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, maurizio.battino@uneatlantico.es (2016) Strawberry consumption alleviates doxorubicin-induced toxicity by suppressing oxidative stress. Food and Chemical Toxicology, 94. pp. 128-137. ISSN 0278-6915

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Cerrado Inglés Glioblastoma multiforme (GBM) are extremely lethal and still poorly treated primary brain tumors, characterized by the presence of highly tumorigenic cancer stem cell (CSC) subpopulations, considered responsible for tumor relapse. In order to successfully eradicate GBM growth and recurrence, new anti-cancer strategies selectively targeting CSCs should be designed. CSCs might be eradicated by targeting some of their cell surface markers and transporters, inducing their differentiation, impacting their hyper-glycolytic metabolism, inhibiting CSC-related signaling pathways and/or by targeting their microenvironmental niche. In this regard, phytocompounds such as curcumin, isothiocyanates, resveratrol and epigallocatechin-3-gallate have been shown to prevent or reverse cancer-related epigenetic dysfunctions, reducing tumorigenesis, preventing metastasis and/or increasing chemotherapy and radiotherapy efficacy. However, the actual bioavailability and metabolic processing of phytocompounds is generally unknown, and the presence of the blood brain barrier often represents a limitation to glioma treatments. Nowadays, nanoparticles (NPs) can be loaded with therapeutic compounds such as phytochemicals, improving their bioavailability and their targeted delivery within the GBM tumor bulk. Moreover, NPs can be designed to increase their tropism and specificity toward CSCs by conjugating their surface with antibodies specific for CSC antigens, with ligands or with glucose analogues. Here we discuss the use of phytochemicals as anti-glioma agents and the applicability of phytochemical-loaded NPs as drug delivery systems to target GBM. Additionally, we provide some examples on how NPs can be specifically formulated to improve CSC targeting. metadata Pistollato, Francesca and Bremer-Hoffmann, Susanne and Basso, Giuseppe and Sumalla Cano, Sandra and Elío Pascual, Iñaki and Masías Vergara, Manuel and Giampieri, Francesca and Battino, Maurizio mail francesca.pistollato@uneatlantico.es, UNSPECIFIED, UNSPECIFIED, sandra.sumalla@uneatlantico.es, inaki.elio@uneatlantico.es, manuel.masias@uneatlantico.es, francesca.giampieri@uneatlantico.es, maurizio.battino@uneatlantico.es (2016) Targeting Glioblastoma with the Use of Phytocompounds and Nanoparticles. Targeted Oncology, 11 (1). pp. 1-16. ISSN 1776-2596

2015

Article Subjects > Biomedicine Europe University of Atlantic > Research > Scientific Production Cerrado Inglés Since a high intake of fruits and vegetables is inversely related to the incidence of several degenerative diseases, the importance of a balanced diet in relation to human health has received increased consumer attention worldwide. Strawberries (Fragaria X ananassa, Duch.) are a rich source of a wide variety of nutritive compounds such as sugars, vitamins, and minerals, as well as non-nutritive, bioactive compounds such as flavonoids, anthocyanins and phenolic acids. All of these compounds exert a synergistic and cumulative effect on human health promotion and in disease prevention. Strawberry phenolics are indeed able (i) to detoxify free radicals blocking their production, (ii) to modulate the expression of genes involved in metabolism, cell survival and proliferation and antioxidant defense, and (iii) to protect and repair DNA damage. The overall objective of the present review is to update and discuss the key findings, from recent in vivo studies, on the effects of strawberries on human health. Particular attention will be paid to the molecular mechanisms proposed to explain the health effects of polyphenols against the most common diseases related to oxidative stress driven pathologies, such as cancer, cardiovascular diseases, type II diabetes, obesity and neurodegenerative diseases, and inflammation. metadata Giampieri, Francesca and Forbes-Hernandez, Tamara Y. and Gasparrini, Massimiliano and Alvarez-Suarez, José M. and Afrin, Sadia and Bompadre, Stefano and Quiles, José L. and Mezzetti, Bruno and Battino, Maurizio mail UNSPECIFIED, tamara.forbes@unini.edu.mx, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, maurizio.battino@uneatlantico.es (2015) Strawberry as a health promoter: an evidence based review. Food & Function, 6 (5). pp. 1386-1398. ISSN 2042-6496

Article Subjects > Biomedicine
Subjects > Nutrition
Europe University of Atlantic > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Ibero-american International University > Research > Scientific Production
Cerrado Inglés In the last decade, specific dietary patterns, mainly characterized by high consumption of vegetables and fruits, have been proven beneficial for the prevention of both metabolic syndrome (MetS)-related dysfunctions and neurodegenerative disorders, such as Alzheimer’s disease (AD). Nowadays, neuroimaging readouts can be used to diagnose AD, investigate MetS effects on brain functionality and anatomy, and assess the effects of dietary supplementations and nutritional patterns in relation to neurodegeneration and AD-related features. Here we review scientific literature describing the use of the most recent neuroimaging techniques to detect AD- and MetS-related brain features, and also to investigate associations between consolidated dietary patterns or nutritional interventions and AD, specifically focusing on observational and intervention studies in humans. metadata Pistollato, Francesca and Sumalla Cano, Sandra and Elío Pascual, Iñaki and Masías Vergara, Manuel and Giampieri, Francesca and Battino, Maurizio mail francesca.pistollato@uneatlantico.es, sandra.sumalla@uneatlantico.es, inaki.elio@uneatlantico.es, manuel.masias@uneatlantico.es, francesca.giampieri@uneatlantico.es, maurizio.battino@uneatlantico.es (2015) The Use of Neuroimaging to Assess Associations Among Diet, Nutrients, Metabolic Syndrome, and Alzheimer’s Disease. Journal of Alzheimer's Disease, 48 (2). pp. 303-318. ISSN 13872877

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Valorization of food products using natural functional compounds for improving organoleptic and functional chemistry

Producción Científica

Maurizio Battino mail maurizio.battino@uneatlantico.es, Tarun Belwal mail , Miguel A. Prieto mail ,

Battino

<a href="/489/1/ijerph-19-00849.pdf" class="ep_document_link"><img class="ep_doc_icon" alt="[img]" src="/489/1.hassmallThumbnailVersion/ijerph-19-00849.pdf" border="0"/></a><a class="ep_document_link" href="/489/1/ijerph-19-00849.pdf"><img class="ep_doc_icon" alt="[img]" src="/489/1.hassmallThumbnailVersion/ijerph-19-00849.pdf" border="0"/></a>

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Detection of Upper Limb Asymmetries in Athletes According to the Stage of the Season—A Longitudinal Study

Abstract: Sports injuries can affect the performance of athletes. For this reason, functional tests are used for injury assessment and prevention, analyzing physical or physiological imbalances and detecting asymmetries. The main aim of this study was to detect the asymmetries in the upper limbs (right and left arms) in athletes, using the OctoBalance Test (OB), depending on the stage of the season. Two hundred and fifty-two participants (age: 23.33 ± 8.96 years old; height: 178.63 ± 11.12 cm; body mass: 80.28 ± 17.61 kg; body mass index: 24.88 ± 4.58; sports experience: 12.52 ± 6.28 years), practicing different sports (rugby, athletics, football, swimming, handball, triathlon, basketball, hockey, badminton and volleyball), assessed with the OB in medial, superolateral, and inferolateral directions in both arms, in four moments of the season (May 2017, September 2017, February 2018 and May 2018). ANOVA test was used with repeated measures with a p ≤ 0.05, for the analysis of the different studied variances. Significant differences were found (p = 0.021) in the medial direction of the left arm, between the first (May 2017) and fourth stages (May 2018), with values of 71.02 ± 7.15 cm and 65.03 ± 7.66 cm. From the detection of asymmetries, using the OB to measure, in the medial, superolateral and inferolateral directions, mobility and balance can be assessed. In addition, it is possible to observe functional imbalances, as a risk factor for injury, in each of the stages into which the season is divided, which will help in the prevention of injuries and in the individualization of training.

Producción Científica

Álvaro Velarde-Sotres mail alvaro.velarde@uneatlantico.es, Antonio Bores-Cerezal mail antonio.bores@uneatlantico.es, Marcos Mecías-Calvo mail marcos.mecias@uneatlantico.es, Martín Barcala Furelos mail martin.barcala@uneatlantico.es, Silvia Aparicio Obregón mail silvia.aparicio@uneatlantico.es, Julio Calleja-González mail ,

Velarde-Sotres

<a href="/490/1/sustainability-14-00913-v2.pdf" class="ep_document_link"><img class="ep_doc_icon" alt="[img]" src="/490/1.hassmallThumbnailVersion/sustainability-14-00913-v2.pdf" border="0"/></a>

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Blockchain Interoperability: Towards a Sustainable Payment System

The highly fragmented blockchain and cryptocurrency ecosystem necessitates interoperability mechanisms as a requirement for blockchain-technology acceptance. The immediate implication of interchain interoperability is automatic swapping between cryptocurrencies. We performed a systematic review of the existing literature on Blockchain interoperability and atomic cross-chain transactions. We investigated different blockchain interoperability approaches, including industrial solutions, categorized them and identified the key mechanisms used, and list several example projects for each category. We focused on the atomic transactions between blockchain, a process also known as atomic swap. Furthermore, we studied recent implementations along with architectural approaches for atomic swap and deduced research issues and challenges in cross-chain interoperability and atomic swap. Atomic swap can instantly transfer tokens and significantly reduce the associated costs without using any centralized authority, and thus facilitates the development of a sustainable payment system for wider financial inclusion.

Producción Científica

Debasis Mohanty mail , Divya Anand mail , Hani Moaiteq Aljahdali mail , Santos Gracia Villar mail santos.gracia@uneatlantico.es,

Mohanty

<a class="ep_document_link" href="/495/1/ijerph-19-01256.pdf"><img class="ep_doc_icon" alt="[img]" src="/495/1.hassmallThumbnailVersion/ijerph-19-01256.pdf" border="0"/></a>

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The Regular Consumption of Nuts Is Associated with a Lower Prevalence of Abdominal Obesity and Metabolic Syndrome in Older People from the North of Spain

Background: The aim of this study was to relate the adherence to nut consumption (30 g) three or more days per week to the prevalence of abdominal obesity and metabolic syndrome (MetS) in an elderly population from the north of Spain. Methods: The study consists of an observational, descriptive, cross-sectional, and correlational study conducted in 556 non-institutionalised individuals between 65 and 79 years of age. To define the consumption recommendation of nuts the indication of the questionnaire MEDAS-14 was followed. The diagnosis of MetS was conducted using the International Diabetes Federation (IDF) criteria. Results: In 264 subjects aged 71.9 (SD: ±4.2) years old, 39% of whom were men, the adherence to nut consumption recommendations was 40.2%. Of these individuals, 79.5% had abdominal obesity. The prevalence of MetS was 40.2%, being 47.6% in men and 35.4% in women (p < 0.05). A nut consumption lower than recommended was associated with a 19% higher prevalence of abdominal obesity (Prevalence Ratio: 1.19; 95% CI: 1.03−1.37; p < 0.05) and a 61% higher prevalence of MetS (Prevalence Ratio: 1.61; 95% CI: 1.16−2.25; p = 0.005) compared to a consumption of ≥3 servings per week. Conclusion: An inverse relationship was established between nut consumption and the prevalence of abdominal obesity and metabolic syndrome.

Producción Científica

Gloria Cubas-Basterrechea mail , Iñaki Elío Pascual mail inaki.elio@uneatlantico.es, Sandra Sumalla Cano mail sandra.sumalla@uneatlantico.es, Silvia Aparicio Obregón mail silvia.aparicio@uneatlantico.es, Carolina Teresa González-Antón mail , Pedro Muñoz-Cacho mail ,

Cubas-Basterrechea

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Effects of ergo-nutritional strategies on recovery in combat sports disciplines

In order to improve the recovery process in combat sports disciplines, ergo-nutritional strategies could be an effective option in training and competition. Some of these ergo-nutritional aids could improve performance but literature references are scarce, with controversial results regarding actual recovery effects. This systematic review aimed to examine which ergo-nutritional methods are most effective for assisting in the recovery process in combat sports, and to determine the appropriate training stimuli. This systematic review was carried out following the Preferred Reporting Items for Systematic Review (PRISMA) guidelines. A computerized search was performed in PubMed, Web of Science, the Cochrane Collaboration Database, Evidence Database, Evidence Based Medicine Search review, National Guidelines, EM-BASE, Scopus and Google Scholar system (from 1995 to April 30, 2021). The PICOS model was used to define inclusion and exclusion criteria. Out of 123 studies initially found, 18 met the eligibility criteria and were included in the review. Data from 367 athletes from different disciplines were examined. The evidence was grouped in 4 areas: oxidative stress, muscle and energy recovery, muscle repair, and metabolic acidosis. Evidence showed that vitamins, minerals, and some natural ergo-nutritional products are effective as antioxidants. Carbohydrates and protein determine the recovery effect. Sodium bicarbonate has a role as primary acidosis metabolic delayer. Accordingly, ergo-nutritional aids can help in the recovery process. Considering the effects outlined in the literature, more studies are needed to provide firm evidence.

Producción Científica

Isaac López Laval mail , Diego Marqués Giménez mail , Álvaro Velarde-Sotres mail alvaro.velarde@uneatlantico.es, Sebastian Sitko mail , Julio Calleja Gonzalez mail ,

López Laval