Metformin and caloric restriction induce an AMPK-dependent restoration of mitochondrial dysfunction in fibroblasts from Fibromyalgia patients

Article Subjects > Nutrition Europe University of Atlantic > Research > Articles and books UNSPECIFIED UNSPECIFIED Impaired AMPK is associated with a wide spectrum of clinical and pathological conditions, ranging from obesity, altered responses to exercise or metabolic syndrome, to inflammation, disturbed mitochondrial biogenesis and defective response to energy stress. Fibromyalgia (FM) is a world-wide diffused musculoskeletal chronic pain condition that affects up to 5% of the general population and comprises all the above mentioned pathophysiological states. Here, we tested the involvement of AMPK activation in fibroblasts derived from FM patients. AMPK was not phosphorylated in fibroblasts from FM patients and was associated with decreased mitochondrial biogenesis, reduced oxygen consumption, decreased antioxidant enzymes expression levels and mitochondrial dysfunction. However, mtDNA sequencing analysis did not show any important alterations which could justify the mitochondrial defects. AMPK activation in FM fibroblast was impaired in response to moderate oxidative stress. In contrast, AMPK activation by metformin or incubation with serum from caloric restricted mice improved the response to moderate oxidative stress and mitochondrial metabolism in FM fibroblasts. These results suggest that AMPK plays an essential role in FM pathophysiology and could represent the basis for a valuable new therapeutic target/strategy. Furthermore, both metformin and caloric restriction could be an interesting therapeutic approach in FM. metadata Alcocer-Gómez, Elísabet and Garrido-Maraver, Juan and Bullón, Pedro and Marín-Aguilar, Fabiola and Cotán, David and Carrión, Angel M. and Alvarez-Suarez, José M. and Giampieri, Francesca and Sánchez-Alcazar, José Antonio and Battino, Maurizio and Cordero, Mario D. mail UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, jose.alvarez@unini.edu.mx, UNSPECIFIED, UNSPECIFIED, maurizio.battino@uneatlantico.es, UNSPECIFIED (2015) Metformin and caloric restriction induce an AMPK-dependent restoration of mitochondrial dysfunction in fibroblasts from Fibromyalgia patients. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1852 (7). pp. 1257-1267. ISSN 0925-4439

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Abstract

Impaired AMPK is associated with a wide spectrum of clinical and pathological conditions, ranging from obesity, altered responses to exercise or metabolic syndrome, to inflammation, disturbed mitochondrial biogenesis and defective response to energy stress. Fibromyalgia (FM) is a world-wide diffused musculoskeletal chronic pain condition that affects up to 5% of the general population and comprises all the above mentioned pathophysiological states. Here, we tested the involvement of AMPK activation in fibroblasts derived from FM patients. AMPK was not phosphorylated in fibroblasts from FM patients and was associated with decreased mitochondrial biogenesis, reduced oxygen consumption, decreased antioxidant enzymes expression levels and mitochondrial dysfunction. However, mtDNA sequencing analysis did not show any important alterations which could justify the mitochondrial defects. AMPK activation in FM fibroblast was impaired in response to moderate oxidative stress. In contrast, AMPK activation by metformin or incubation with serum from caloric restricted mice improved the response to moderate oxidative stress and mitochondrial metabolism in FM fibroblasts. These results suggest that AMPK plays an essential role in FM pathophysiology and could represent the basis for a valuable new therapeutic target/strategy. Furthermore, both metformin and caloric restriction could be an interesting therapeutic approach in FM.

Item Type: Article
Uncontrolled Keywords: Fibromyalgia; AMPK; Mitochondria; Oxidative stress; Metformin; Caloric restriction.
Subjects: Subjects > Nutrition
Divisions: Europe University of Atlantic > Research > Articles and books
SWORD Depositor: Users 0 not found.
Date Deposited: 31 May 2021 14:17
Last Modified: 30 Jun 2021 15:38
URI: https://repositorio.uneatlantico.es/id/eprint/97

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  • Metformin and caloric restriction induce an AMPK-dependent restoration of mitochondrial dysfunction in fibroblasts from Fibromyalgia patients. (deposited 31 May 2021 14:17) [Currently Displayed]

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  • Alcocer-Gómez, Elísabet and Garrido-Maraver, Juan and Bullón, Pedro and Marín-Aguilar, Fabiola and Cotán, David and Carrión, Angel M. and Alvarez-Suarez, José M. and Giampieri, Francesca and Sánchez-Alcazar, José Antonio and Battino, Maurizio and Cordero, Mario D. Metformin and caloric restriction induce an AMPK-dependent restoration of mitochondrial dysfunction in fibroblasts from Fibromyalgia patients. (deposited 31 May 2021 14:17) [Currently Displayed]

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