Anti-inflammatory effect of strawberry extract against LPS-induced stress in RAW 264.7 macrophages
Article
Subjects > Biomedicine
Europe University of Atlantic > Research > Articles and books
Ibero-american International University > Research > Scientific Production
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A common denominator in the pathogenesis of most chronic inflammatory diseases is the involvement of oxidative stress, related to ROS production by all aerobic organisms. Dietary antioxidants from plant foods represent an efficient strategy to counteract this condition. The aim of the present study was to evaluate the protective effects of strawberry extracts on inflammatory status induced by E. Coli LPS on RAW 264.7 macrophages by measuring the main oxidative and inflammatory biomarkers and investigating the molecular pathways involved. Strawberry pre-treatment efficiently counteracted LPS-induced oxidative stress reducing the amount of ROS and nitrite production, stimulating endogenous antioxidant enzyme activities and enhancing protection against lipid, protein and DNA damage (P < 0.05). Strawberry pre-treatment exerted these protective effects primarily through the activation of the Nrf2 pathway, which is markedly AMPK-dependent and also by the modulation of the NF-kB signalling pathway. Finally, an improvement in mitochondria functionality was also detected. The results obtained in this work highlight the health benefit of strawberries against inflammatory and oxidative stress in LPS-stimulated RAW 264.7 macrophages, investigating for the first time the possible involved molecular mechanisms.
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Gasparrini, Massimiliano and Forbes-Hernandez, Tamara Y. and Giampieri, Francesca and Afrin, Sadia and Alvarez-Suarez, Josè M. and Mazzoni, Luca and Mezzetti, Bruno and Quiles, Josè L. and Battino, Maurizio
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UNSPECIFIED, tamara.forbes@unini.edu.mx, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, UNSPECIFIED, maurizio.battino@uneatlantico.es
(2017)
Anti-inflammatory effect of strawberry extract against LPS-induced stress in RAW 264.7 macrophages.
Food and Chemical Toxicology, 102.
pp. 1-10.
ISSN 0278-6915
This is the latest version of this item.
Abstract
A common denominator in the pathogenesis of most chronic inflammatory diseases is the involvement of oxidative stress, related to ROS production by all aerobic organisms. Dietary antioxidants from plant foods represent an efficient strategy to counteract this condition. The aim of the present study was to evaluate the protective effects of strawberry extracts on inflammatory status induced by E. Coli LPS on RAW 264.7 macrophages by measuring the main oxidative and inflammatory biomarkers and investigating the molecular pathways involved. Strawberry pre-treatment efficiently counteracted LPS-induced oxidative stress reducing the amount of ROS and nitrite production, stimulating endogenous antioxidant enzyme activities and enhancing protection against lipid, protein and DNA damage (P < 0.05). Strawberry pre-treatment exerted these protective effects primarily through the activation of the Nrf2 pathway, which is markedly AMPK-dependent and also by the modulation of the NF-kB signalling pathway. Finally, an improvement in mitochondria functionality was also detected. The results obtained in this work highlight the health benefit of strawberries against inflammatory and oxidative stress in LPS-stimulated RAW 264.7 macrophages, investigating for the first time the possible involved molecular mechanisms.
Item Type: | Article |
---|---|
Uncontrolled Keywords: | Strawberry; Phenolic compounds; LPS; Inflammation; ROS; Prevention. |
Subjects: | Subjects > Biomedicine |
Divisions: | Europe University of Atlantic > Research > Articles and books Ibero-american International University > Research > Scientific Production |
SWORD Depositor: | Users 0 not found. |
Date Deposited: | 02 Jun 2021 23:55 |
Last Modified: | 03 Mar 2022 23:55 |
URI: | https://repositorio.uneatlantico.es/id/eprint/142 |
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- Anti-inflammatory effect of strawberry extract against LPS-induced stress in RAW 264.7 macrophages. (deposited 02 Jun 2021 23:55) [Currently Displayed]
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- Gasparrini, Massimiliano and Forbes-Hernandez, Tamara Y. and Giampieri, Francesca and Afrin, Sadia and Alvarez-Suarez, Josè M. and Mazzoni, Luca and Mezzetti, Bruno and Quiles, Josè L. and Battino, Maurizio Anti-inflammatory effect of strawberry extract against LPS-induced stress in RAW 264.7 macrophages. (deposited 02 Jun 2021 23:55) [Currently Displayed]
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