Double-Stranded RNA Targeting Dicer-Like Genes Compromises the Pathogenicity of Plasmopara viticola on Grapevine

Article Subjects > Nutrition Europe University of Atlantic > Research > Scientific Production Abierto Inglés metadata Haile, Zeraye Mehari and Gebremichael, Daniel Endale and Capriotti, Luca and Molesini, Barbara and Negrini, Francesca and Collina, Marina and Sabbadini, Silvia and Mezzetti, Bruno and Baraldi, Elena mail UNSPECIFIED (2021) Double-Stranded RNA Targeting Dicer-Like Genes Compromises the Pathogenicity of Plasmopara viticola on Grapevine. Frontiers in Plant Science, 12. ISSN 1664-462X

[img]
Preview
Text
fpls-12-667539.pdf - Published Version
Available under License Creative Commons Attribution.

Download (3MB) | Preview

Abstract

Downy mildew caused by Plasmopara viticola is one of the most devastating diseases of grapevine, attacking all green parts of the plant. The damage is severe when the infection at flowering stage is left uncontrolled. P. viticola management consumes a significant amount of classical pesticides applied in vineyards, requiring efficient and environmentally safe disease management options. Spray-induced gene silencing (SIGS), through the application of exogenous double-stranded RNA (dsRNA), has shown promising results for the management of diseases in crops. Here, we developed and tested the potential of dsRNA targeting P. viticola Dicer-like (DCL) genes for SIGS-based crop protection strategy. The exogenous application of PvDCL1/2 dsRNA, a chimera of PvDCL1 and PvDCL2, highly affected the virulence of P. viticola. The reduced expression level of PvDCL1 and PvDCL2 transcripts in infected leaves, treated with PvDCL1/2 dsRNA, was an indication of an active RNA interference mechanism inside the pathogen to compromise its virulence. Besides the protective property, the PvDCL1/2 dsRNA also exhibited a curative role by reducing the disease progress rate of already established infection. Our data provide a promising future for PvDCL1/2 dsRNA as a new generation of RNA-based resistant plants or RNA-based agrochemical for the management of downy mildew disease in grapevine.

Item Type: Article
Uncontrolled Keywords: Dicer-like genes; double-stranded RNA (dsRNA); Plasmopara viticola; spray-induced gene silencing; Vitis vinifera
Subjects: Subjects > Nutrition
Divisions: Europe University of Atlantic > Research > Scientific Production
Date Deposited: 22 Jul 2021 02:49
Last Modified: 03 May 2022 19:03
URI: https://repositorio.uneatlantico.es/id/eprint/266

Actions (login required)

View Item View Item

<a href="/489/1/ijerph-19-00849.pdf" class="ep_document_link"><img class="ep_doc_icon" alt="[img]" src="/489/1.hassmallThumbnailVersion/ijerph-19-00849.pdf" border="0"/></a><a class="ep_document_link" href="/489/1/ijerph-19-00849.pdf"><img class="ep_doc_icon" alt="[img]" src="/489/1.hassmallThumbnailVersion/ijerph-19-00849.pdf" border="0"/></a>

Detection of Upper Limb Asymmetries in Athletes According to the Stage of the Season—A Longitudinal Study

Abstract: Sports injuries can affect the performance of athletes. For this reason, functional tests are used for injury assessment and prevention, analyzing physical or physiological imbalances and detecting asymmetries. The main aim of this study was to detect the asymmetries in the upper limbs (right and left arms) in athletes, using the OctoBalance Test (OB), depending on the stage of the season. Two hundred and fifty-two participants (age: 23.33 ± 8.96 years old; height: 178.63 ± 11.12 cm; body mass: 80.28 ± 17.61 kg; body mass index: 24.88 ± 4.58; sports experience: 12.52 ± 6.28 years), practicing different sports (rugby, athletics, football, swimming, handball, triathlon, basketball, hockey, badminton and volleyball), assessed with the OB in medial, superolateral, and inferolateral directions in both arms, in four moments of the season (May 2017, September 2017, February 2018 and May 2018). ANOVA test was used with repeated measures with a p ≤ 0.05, for the analysis of the different studied variances. Significant differences were found (p = 0.021) in the medial direction of the left arm, between the first (May 2017) and fourth stages (May 2018), with values of 71.02 ± 7.15 cm and 65.03 ± 7.66 cm. From the detection of asymmetries, using the OB to measure, in the medial, superolateral and inferolateral directions, mobility and balance can be assessed. In addition, it is possible to observe functional imbalances, as a risk factor for injury, in each of the stages into which the season is divided, which will help in the prevention of injuries and in the individualization of training.

Producción Científica

Álvaro Velarde-Sotres mail alvaro.velarde@uneatlantico.es, Antonio Bores-Cerezal mail antonio.bores@uneatlantico.es, Marcos Mecías-Calvo mail marcos.mecias@uneatlantico.es, Martín Barcala Furelos mail martin.barcala@uneatlantico.es, Silvia Aparicio Obregón mail silvia.aparicio@uneatlantico.es, Julio Calleja-González mail ,

Velarde-Sotres

<a href="/490/1/sustainability-14-00913-v2.pdf" class="ep_document_link"><img class="ep_doc_icon" alt="[img]" src="/490/1.hassmallThumbnailVersion/sustainability-14-00913-v2.pdf" border="0"/></a>

Blockchain Interoperability: Towards a Sustainable Payment System

The highly fragmented blockchain and cryptocurrency ecosystem necessitates interoperability mechanisms as a requirement for blockchain-technology acceptance. The immediate implication of interchain interoperability is automatic swapping between cryptocurrencies. We performed a systematic review of the existing literature on Blockchain interoperability and atomic cross-chain transactions. We investigated different blockchain interoperability approaches, including industrial solutions, categorized them and identified the key mechanisms used, and list several example projects for each category. We focused on the atomic transactions between blockchain, a process also known as atomic swap. Furthermore, we studied recent implementations along with architectural approaches for atomic swap and deduced research issues and challenges in cross-chain interoperability and atomic swap. Atomic swap can instantly transfer tokens and significantly reduce the associated costs without using any centralized authority, and thus facilitates the development of a sustainable payment system for wider financial inclusion.

Producción Científica

Debasis Mohanty mail , Divya Anand mail , Hani Moaiteq Aljahdali mail , Santos Gracia Villar mail santos.gracia@uneatlantico.es,

Mohanty

<a class="ep_document_link" href="/495/1/ijerph-19-01256.pdf"><img class="ep_doc_icon" alt="[img]" src="/495/1.hassmallThumbnailVersion/ijerph-19-01256.pdf" border="0"/></a>

The Regular Consumption of Nuts Is Associated with a Lower Prevalence of Abdominal Obesity and Metabolic Syndrome in Older People from the North of Spain

Background: The aim of this study was to relate the adherence to nut consumption (30 g) three or more days per week to the prevalence of abdominal obesity and metabolic syndrome (MetS) in an elderly population from the north of Spain. Methods: The study consists of an observational, descriptive, cross-sectional, and correlational study conducted in 556 non-institutionalised individuals between 65 and 79 years of age. To define the consumption recommendation of nuts the indication of the questionnaire MEDAS-14 was followed. The diagnosis of MetS was conducted using the International Diabetes Federation (IDF) criteria. Results: In 264 subjects aged 71.9 (SD: ±4.2) years old, 39% of whom were men, the adherence to nut consumption recommendations was 40.2%. Of these individuals, 79.5% had abdominal obesity. The prevalence of MetS was 40.2%, being 47.6% in men and 35.4% in women (p < 0.05). A nut consumption lower than recommended was associated with a 19% higher prevalence of abdominal obesity (Prevalence Ratio: 1.19; 95% CI: 1.03−1.37; p < 0.05) and a 61% higher prevalence of MetS (Prevalence Ratio: 1.61; 95% CI: 1.16−2.25; p = 0.005) compared to a consumption of ≥3 servings per week. Conclusion: An inverse relationship was established between nut consumption and the prevalence of abdominal obesity and metabolic syndrome.

Producción Científica

Gloria Cubas-Basterrechea mail , Iñaki Elío Pascual mail inaki.elio@uneatlantico.es, Sandra Sumalla Cano mail sandra.sumalla@uneatlantico.es, Silvia Aparicio Obregón mail silvia.aparicio@uneatlantico.es, Carolina Teresa González-Antón mail , Pedro Muñoz-Cacho mail ,

Cubas-Basterrechea

<a class="ep_document_link" href="/496/1/CO-WM-03886-02.pdf"><img class="ep_doc_icon" alt="[img]" src="/496/1.hassmallThumbnailVersion/CO-WM-03886-02.pdf" border="0"/></a><a class="ep_document_link" href="/496/1/CO-WM-03886-02.pdf"><img class="ep_doc_icon" alt="[img]" src="/496/1.hassmallThumbnailVersion/CO-WM-03886-02.pdf" border="0"/></a>

Effects of ergo-nutritional strategies on recovery in combat sports disciplines

In order to improve the recovery process in combat sports disciplines, ergo-nutritional strategies could be an effective option in training and competition. Some of these ergo-nutritional aids could improve performance but literature references are scarce, with controversial results regarding actual recovery effects. This systematic review aimed to examine which ergo-nutritional methods are most effective for assisting in the recovery process in combat sports, and to determine the appropriate training stimuli. This systematic review was carried out following the Preferred Reporting Items for Systematic Review (PRISMA) guidelines. A computerized search was performed in PubMed, Web of Science, the Cochrane Collaboration Database, Evidence Database, Evidence Based Medicine Search review, National Guidelines, EM-BASE, Scopus and Google Scholar system (from 1995 to April 30, 2021). The PICOS model was used to define inclusion and exclusion criteria. Out of 123 studies initially found, 18 met the eligibility criteria and were included in the review. Data from 367 athletes from different disciplines were examined. The evidence was grouped in 4 areas: oxidative stress, muscle and energy recovery, muscle repair, and metabolic acidosis. Evidence showed that vitamins, minerals, and some natural ergo-nutritional products are effective as antioxidants. Carbohydrates and protein determine the recovery effect. Sodium bicarbonate has a role as primary acidosis metabolic delayer. Accordingly, ergo-nutritional aids can help in the recovery process. Considering the effects outlined in the literature, more studies are needed to provide firm evidence.

Producción Científica

Isaac López Laval mail , Diego Marqués Giménez mail , Álvaro Velarde-Sotres mail alvaro.velarde@uneatlantico.es, Sebastian Sitko mail , Julio Calleja Gonzalez mail ,

López Laval

<a class="ep_document_link" href="/499/1/s12645-021-00107-6.pdf"><img class="ep_doc_icon" alt="[img]" src="/499/1.hassmallThumbnailVersion/s12645-021-00107-6.pdf" border="0"/></a>

Non-homogeneous dispersion of graphene in polyacrylonitrile substrates induces a migrastatic response and epithelial-like differentiation in MCF7 breast cancer cells

Background Recent advances from studies of graphene and graphene-based derivatives have highlighted the great potential of these nanomaterials as migrastatic agents with the ability to modulate tumor microenvironments. Nevertheless, the administration of graphene nanomaterials in suspensions in vivo is controversial. As an alternative approach, herein, we report the immobilization of high concentrations of graphene nanoplatelets in polyacrylonitrile film substrates (named PAN/G10) and evaluate their potential use as migrastatic agents on cancer cells. Results Breast cancer MCF7 cells cultured on PAN/G10 substrates presented features resembling mesenchymal-to-epithelial transition, e.g., (i) inhibition of migratory activity; (ii) activation of the expression of E-cadherin, cytokeratin 18, ZO-1 and EpCAM, four key molecular markers of epithelial differentiation; (iii) formation of adherens junctions with clustering and adhesion of cancer cells in aggregates or islets, and (iv) reorganization of the actin cytoskeleton resulting in a polygonal cell shape. Remarkably, assessment with Raman spectroscopy revealed that the above-mentioned events were produced when MCF7 cells were preferentially located on top of graphene-rich regions of the PAN/G10 substrates. Conclusions The present data demonstrate the capacity of these composite substrates to induce an epithelial-like differentiation in MCF7 breast cancer cells, resulting in a migrastatic effect without any chemical agent-mediated signaling. Future works will aim to thoroughly evaluate the mechanisms of how PAN/G10 substrates trigger these responses in cancer cells and their potential use as antimetastatics for the treatment of solid cancers.

Producción Científica

Nazely Diban mail , Marián Mantecón-Oria mail , María T. Berciano mail , Alba Puente-Bedia mail , María J. Rivero mail , Ane Urtiaga mail , Miguel Lafarga mail , Olga Tapia Martínez mail olga.tapia@uneatlantico.es,

Diban