TY - JOUR Y1 - 2021/04// A1 - Ansary, Johura A1 - Giampieri, Francesca A1 - Forbes-Hernandez, Tamara Y. A1 - Regolo, Lucia A1 - Quinzi, Denise A1 - Gracia Villar, Santos A1 - Garcia Villena, Eduardo A1 - Tutusaus, Kilian A1 - Alvarez-Suarez, José M. A1 - Battino, Maurizio A1 - Cianciosi, Danila SN - 1420-3049 JF - Molecules N2 - In recent times, scientific attention has been paid to different foods and their bioactive components for the ability to inhibit the onset and progress of different types of cancer. Nigella sativa extract, powder and seed oil and its main components, thymoquinone and ?-hederin, have showed potent anticancer and chemosensitizing effects against various types of cancer, such as liver, colon, breast, renal, cervical, lung, ovarian, pancreatic, prostate and skin tumors, through the modulation of various molecular signaling pathways. Herein, the purpose of this review was to highlight the anticancer activity of Nigella sativa and it constitutes, focusing on different in vitro, in vivo and clinical studies and projects, in order to underline their antiproliferative, proapoptotic, cytotoxic and antimetastatic effects. Particular attention has been also given to the synergistic effect of Nigella sativa and it constitutes with chemotherapeutic drugs, and to the synthesized analogs of thymoquinone that seem to enhance the chemo-sensitizing potential. This review could be a useful step towards new research on N. sativa and cancer, to include this plant in the dietary treatments in support to conventional therapies, for the best achievement of therapeutic goals. VL - 26 ID - uneatlantico77 TI - Nutritional Value and Preventive Role of Nigella sativa L. and Its Main Component Thymoquinone in Cancer: An Evidenced-Based Review of Preclinical and Clinical Studies IS - 8 UR - http://doi.org/10.3390/molecules26082108 AV - public KW - Nigella sativa KW - Nutritional composition KW - Anticancer properties KW - Molecular pathways KW - Combined therapy. ER - TY - JOUR N2 - The Papillon?Lefèvre syndrome (PLS) is a rare autosomal recessive disorder caused by mutations in the Cathepsin C (CTSC) gene, characterized by periodontitis and palmoplantar hyperkeratosis. The main inflammatory deficiencies include oxidative stress and autophagic dysfunction. Mitochondria are the main source of reactive oxygen species; their impaired function is related to skin diseases and periodontitis. The mitochondrial function has been evaluated in PLS and mitochondria have been targeted as a possible treatment for PLS. We show for the first time an important mitochondrial dysfunction associated with increased oxidative damage of mtDNA, reduced CoQ10 and mitochondrial mass and aberrant morphologies of the mitochondria in PLS patients. Mitochondrial dysfunction, determined by oxygen consumption rate (OCR) in PLS fibroblasts, was treated with CoQ10 supplementation, which determined an improvement in OCR and a remission of skin damage in a patient receiving a topical administration of a cream enriched with CoQ10 0.1%. We provide the first evidence of the role of mitochondrial dysfunction and CoQ10 deficiency in the pathophysiology of PLS and a future therapeutic option for PLS. VL - 10 JF - Antioxidants SN - 2076-3921 A1 - Castejón-Vega, Beatriz A1 - Battino, Maurizio A1 - Quiles, José L. A1 - Bullon, Beatriz A1 - Cordero, Mario D. A1 - Bullón, Pedro Y1 - 2021/01// KW - Papillon?Lefèvre syndrome KW - Mitochondria KW - Coenzyme Q10. UR - http://doi.org/10.3390/antiox10010095 AV - public ID - uneatlantico104 TI - Potential Role of the Mitochondria for the Dermatological Treatment of Papillon-Lefèvre IS - 1 ER - TY - JOUR Y1 - 2021/04// A1 - Cianciosi, Danila A1 - Ansary, Johura A1 - Forbes-Hernandez, Tamara Y. A1 - Regolo, Lucia A1 - Quinzi, Denise A1 - Gracia Villar, Santos A1 - Garcia Villena, Eduardo A1 - Tutusaus Pifarre, Kilian A1 - Alvarez-Suarez, José M. A1 - Battino, Maurizio A1 - Giampieri, Francesca JF - Molecules SN - 1420-3049 N2 - Cancer stem cells (CSCs) are a rare tumor subpopulation with high differentiation, proliferative and tumorigenic potential compared to the remaining tumor population. CSCs were first discovered by Bonnet and Dick in 1997 in acute myeloid leukemia. The identification and isolation of these cells in this pioneering study were carried out through the flow cytometry, exploiting the presence of specific cell surface molecular markers (CD34+/CD38?). In the following years, different strategies and projects have been developed for the study of CSCs, which are basically divided into surface markers assays and functional assays; some of these techniques also allow working with a cellular model that better mimics the tumor architecture. The purpose of this mini review is to summarize and briefly describe all the current methods used for the identification, isolation and enrichment of CSCs, describing, where possible, the molecular basis, the advantages and disadvantages of each technique with a particular focus on those that offer a three-dimensional culture. VL - 26 IS - 9 ID - uneatlantico105 TI - The Molecular Basis of Different Approaches for the Study of Cancer Stem Cells and the Advantages and Disadvantages of a Three-Dimensional Culture AV - public UR - http://doi.org/10.3390/molecules26092615 KW - Cancer stem cells KW - Identification KW - Isolation KW - Enrichment KW - Methodology KW - Three-dimensional culture KW - Surface molecular markers KW - Functional assays. ER - TY - JOUR SN - 0278-6915 JF - Food and Chemical Toxicology VL - 149 N2 - Edible flowers are being used as a new ingredient in modern gastronomy. Recently, these products have also gained interest as an important source of phenolic compounds with potential for biomedical applications. The present work studied a methanolic extract of Rosa x hybrida in which 35 individual phenolic compounds were identified. The extract has been evaluated for its antiproliferative properties in ovarian carcinoma cells. Results showed that the antiproliferative effect was associated with the induction of autophagy and apoptosis with the concomitant ROS increase probably related to mitochondria dysfunction. These antiproliferative effects might be associated with some components of the extract such as quercetin. The extract did not induce damage in healthy cells and that it was able to improve the wound healing activity. The present study also evaluated the properties of the mentioned extract in vivo in C. elegans. Tests demonstrated a lack of toxicity in the worm model. Promising results have been obtained in transgenic strains of C. elegans that produce human beta amyloid peptide, suggesting the possible utility of the extract from the point of view of Alzheimer disease. Altogether, results suggest that Rosa x hybrida extracts could be a new tool for the development of functional foods. Y1 - 2021/// A1 - Rivas-García, Lorenzo A1 - Quiles, José L. A1 - Roma-Rodrigues, Catarina A1 - Raposo, Luis R. A1 - Navarro-Hortal, María D. A1 - Romero-Márquez, Jose M. A1 - Esteban-Muñoz, Adelaida A1 - Varela-López, Alfonso A1 - García, Laura Carrera A1 - Cianciosi, Danila A1 - Forbes Hernandez, Tamara Y. A1 - Battino, Maurizio A1 - Llopis, Juan A1 - Fernandes, Alexandra R. A1 - Baptista, Pedro V. A1 - Sánchez-González, Cristina KW - Antioxidants; Autophagy; Edible flowers; Flavonoids; Polyphenols; ROS. TI - Rosa x hybrida extracts with dual actions: Antiproliferative effects against tumour cells and inhibitor of Alzheimer disease ID - uneatlantico108 UR - http://doi.org/10.1016/j.fct.2021.112018 AV - none ER - TY - JOUR KW - Copy number KW - Metals KW - Mitochondria KW - MtDNA deletions KW - Nanotechnology KW - Respiration. IS - 1 ID - uneatlantico109 TI - Ultra-Small Iron Nanoparticles Target Mitochondria Inducing Autophagy, Acting on Mitochondrial DNA and Reducing Respiration AV - public UR - http://doi.org/10.3390/pharmaceutics13010090 JF - Pharmaceutics SN - 1999-4923 VL - 13 N2 - The application of metallic nanoparticles (materials with size at least in one dimension ranging from 1 to 100 nm) as a new therapeutic tool will improve the diagnosis and treatment of diseases. The mitochondria could be a therapeutic target to treat pathologies whose origin lies in mitochondrial dysfunctions or whose progression is dependent on mitochondrial function. We aimed to study the subcellular distribution of 2-4 nm iron nanoparticles and its effect on mitochondrial DNA (mtDNA), mitochondrial function, and autophagy in colorectal cell lines (HT-29). Results showed that when cells were exposed to ultra-small iron nanoparticles, their subcellular fate was mainly mitochondria, affecting its respiratory and glycolytic parameters, inducing the migration of the cellular state towards quiescence, and promoting and triggering the autophagic process. These effects support the potential use of nanoparticles as therapeutic agents using mitochondria as a target for cancer and other treatments for mitochondria-dependent pathologies. Y1 - 2021/01// A1 - Rivas-García, Lorenzo A1 - Quiles, José L. A1 - Varela-López, Alfonso A1 - Giampieri, Francesca A1 - Battino, Maurizio A1 - Bettmer, Jörg A1 - Montes-Bayón, María A1 - Llopis, Juan A1 - Sánchez-González, Cristina ER - TY - JOUR UR - http://doi.org/10.1016/j.semcancer.2021.01.001 AV - none TI - The central role of mitochondria in the relationship between dietary lipids and cancer progression ID - uneatlantico110 KW - Apoptosis; Autophagy; Bioenergetics; Lipogenesis; Redox biology. A1 - Varela-López, Alfonso A1 - Vera-Ramírez, Laura A1 - Giampieri, Francesca A1 - Navarro-Hortal, María D. A1 - Forbes-Hernandez, Tamara Y. A1 - Battino, Maurizio A1 - Quiles, José L. Y1 - 2021/08// N2 - Evidence demonstrates the importance of lipid metabolism and signaling in cancer cell biology. De novo lipogenesis is an important source of lipids for cancer cells, but exogenous lipid uptake remains essential for many cancer cells. Dietary lipids can modify lipids present in tumor microenvironment affecting cancer cell metabolism. Clinical trials have shown that diets rich in polyunsaturated fatty acids (PUFA) can negatively affect tumor growth. However, certain n-6 PUFAs can also contribute to cancer progression. Identifying the molecular mechanisms through which lipids affect cancer progression will provide an opportunity for focused dietary interventions that could translate into the development of personalized diets for cancer control. However, the effective mechanisms of action of PUFAs have not been fully clarified yet. Mitochondria controls ATP generation, redox homeostasis, metabolic signaling, apoptotic pathways and many aspects of autophagy, and it has been recognized to play a key role in cancer. The purpose of this review is to summarize the current evidence linking dietary lipids effects on mitochondrial aspects with consequences for cancer progression and the molecular mechanisms that underlie this association. EP - 0 SN - 1044579X JF - Seminars in Cancer Biology ER - TY - JOUR JF - Nutrients SN - 2072-6643 VL - 12 N2 - The interaction between nutrition and human infectious diseases has always been recognized. With the emergence of molecular tools and post-genomics, high-resolution sequencing technologies, the gut microbiota has been emerging as a key moderator in the complex interplay between nutrients, human body, and infections. Much of the host?microbial and nutrition research is currently based on animals or simplistic in vitro models. Although traditional in vivo and in vitro models have helped to develop mechanistic hypotheses and assess the causality of the host?microbiota interactions, they often fail to faithfully recapitulate the complexity of the human nutrient?microbiome axis in gastrointestinal homeostasis and infections. Over the last decade, remarkable progress in tissue engineering, stem cell biology, microfluidics, sequencing technologies, and computing power has taken place, which has produced a new generation of human-focused, relevant, and predictive tools. These tools, which include patient-derived organoids, organs-on-a-chip, computational analyses, and models, together with multi-omics readouts, represent novel and exciting equipment to advance the research into microbiota, infectious diseases, and nutrition from a human-biology-based perspective. After considering some limitations of the conventional in vivo and in vitro approaches, in this review, we present the main novel available and emerging tools that are suitable for designing human-oriented research. Y1 - 2020/06// A1 - Cassotta, Manuela A1 - Forbes-Hernández, Tamara Y. A1 - Calderón Iglesias, Ruben A1 - Ruiz, Roberto A1 - Elexpuru Zabaleta, Maria A1 - Giampieri, Francesca A1 - Battino, Maurizio KW - Macrobiotica KW - Infectious diseases KW - Nutrition KW - Human-bases methods KW - Gut-on-a-chip KW - gut-organoids KW - Third generation sequencing. IS - 6 TI - Links between Nutrition, Infectious Diseases, and Microbiota: Emerging Technologies and Opportunities for Human-Focused Research ID - uneatlantico78 AV - none UR - http://doi.org/10.3390/nu12061827 ER - TY - JOUR TI - Pharmacological, non-pharmacological and stem cell therapies for the management of autism spectrum disorders: A focus on human studies ID - uneatlantico125 UR - http://doi.org/10.1016/j.phrs.2019.104579 AV - none KW - Autism spectrum disorder; Pharmacological interventions; Non-Pharmacological strategies; Stem cell therapies; Dietary supplementations; Exclusion diets. Y1 - 2020/02// A1 - Pistollato, Francesca A1 - Forbes-Hernandez, Tamara Y. A1 - Calderón Iglesias, Rubén A1 - Ruiz Salces, Roberto A1 - Elexpuru Zabaleta, Maria A1 - Cianciosi, Danila A1 - Giampieri, Francesca A1 - Battino, Maurizio JF - Pharmacological Research SN - 1043-6618 N2 - In the last decade, the prevalence of autism spectrum disorders (ASD) has dramatically escalated worldwide. Currently available drugs mainly target some co-occurring symptoms of ASD, but are not effective on the core symptoms, namely impairments in communication and social interaction, and the presence of restricted and repetitive behaviors. On the other hand, transplantation of hematopoietic and mesenchymal stem cells in ASD children has been shown promising to stimulate the recruitment, proliferation, and differentiation of tissue-residing native stem cells, reducing inflammation, and improving some ASD symptoms. Moreover, several comorbidities have also been associated with ASD, such as immune dysregulation, gastrointestinal issues and gut microbiota dysbiosis. Non-pharmacological approaches, such as dietary supplementations with certain vitamins, omega-3 polyunsaturated fatty acids, probiotics, some phytochemicals (e.g., luteolin and sulforaphane), or overall diet interventions (e.g., gluten free and casein free diets) have been considered for the reduction of such comorbidities and the management of ASD. Here, interventional studies describing pharmacological and non-pharmacological treatments in ASD children and adolescents, along with stem cell-based therapies, are reviewed. VL - 152 ER - TY - JOUR EP - 57 N2 - Fasting, caloric restriction and foods or compounds mimicking the biological effects of caloric restriction, known as caloric restriction mimetics, have been associated with a lower risk of age-related diseases, including cardiovascular diseases, cancer and cognitive decline, and a longer lifespan. Reduced calorie intake has been shown to stimulate cancer immunosurveillance, reducing the migration of immunosuppressive regulatory T cells towards the tumor bulk. Autophagy stimulation via reduction of lysine acetylation, increased sensitivity to chemo- and immunotherapy, along with a reduction of insulin-like growth factor 1 and reactive oxygen species have been described as some of the major effects triggered by caloric restriction. Fasting and caloric restriction have also been shown to beneficially influence gut microbiota composition, modify host metabolism, reduce total cholesterol and triglyceride levels, lower diastolic blood pressure and elevate morning cortisol level, with beneficial modulatory effects on cardiopulmonary fitness, body fat and weight, fatigue and weakness, and general quality of life. Moreover, caloric restriction may reduce the carcinogenic and metastatic potential of cancer stem cells, which are generally considered responsible of tumor formation and relapse. Here, we reviewed in vitro and in vivo studies describing the effects of fasting, caloric restriction and some caloric restriction mimetics on immunosurveillance, gut microbiota, metabolism, and cancer stem cell growth, highlighting the molecular and cellular mechanisms underlying these effects. Additionally, studies on caloric restriction interventions in cancer patients or cancer risk subjects are discussed. Considering the promising effects associated with caloric restriction and caloric restriction mimetics, we think that controlled-randomized large clinical trials are warranted to evaluate the inclusion of these non-pharmacological approaches in clinical practice. SP - 45 JF - Seminars in Cancer Biology SN - 1044-579X A1 - Pistollato, Francesca A1 - Forbes-Hernández, Tamara Y. A1 - Calderón Iglesias, Rubén A1 - Ruiz Salces, Roberto A1 - Elexpuru Zabaleta, Maria A1 - Dominguez Azpíroz, Irma A1 - Cianciosi, Danila A1 - Quiles, José L. A1 - Giampieri, Francesca A1 - Battino, Maurizio Y1 - 2021/08// KW - Caloric restriction; Fasting; Autophagy; Immune system; Gut microbiota; Cancer stem cells. AV - none UR - http://doi.org/10.1016/j.semcancer.2020.11.017 ID - uneatlantico126 TI - Effects of caloric restriction on immunosurveillance, microbiota and cancer cell phenotype: Possible implications for cancer treatment ER - TY - JOUR KW - Atherogenic; Atherosclerosis; NASH; Non-alcoholic fatty liver disease; Periodontal diseases; Periodontitis; Rabbits. AV - public UR - http://doi.org/10.3390/nu12051405 IS - 5 TI - A Diet Rich in Saturated Fat and Cholesterol Aggravates the Effect of Bacterial Lipopolysaccharide on Alveolar Bone Loss in a Rabbit Model of Periodontal Disease ID - uneatlantico117 VL - 12 N2 - ncreasing evidence connects periodontitis with a variety of systemic diseases, including metabolic syndrome, atherosclerosis, and non-alcoholic fatty liver disease (NAFLD). The proposal of this study was to evaluate the role of diets rich in saturated fat and cholesterol in some aspects of periodontal diseases in a lipopolysaccharide (LPS)-induced model of periodontal disease in rabbits and to assess the influence of a periodontal intervention on hyperlipidemia, atherosclerosis, and NAFLD progression to non-alcoholic steatohepatitis. Male rabbits were maintained on a commercial standard diet or a diet rich in saturated fat (3% lard w/w) and cholesterol (1.3% w/w) (HFD) for 40 days. Half of the rabbits on each diet were treated 2 days per week with intragingival injections of LPS from Porphyromonas gingivalis. Morphometric analyses revealed that LPS induced higher alveolar bone loss (ABL) around the first premolar in animals receiving standard diets, which was exacerbated by the HFD diet. A higher score of acinar inflammation in the liver and higher blood levels of triglycerides and phospholipids were found in HFD-fed rabbits receiving LPS. These results suggest that certain dietary habits can exacerbate some aspects of periodontitis and that bad periodontal health can contribute to dyslipidemia and promote NAFLD progression, but only under certain conditions. JF - Nutrients SN - 2072-6643 A1 - Varela-López, Alfonso A1 - Bullón, Pedro A1 - Ramírez-Tortosa, César L. A1 - Navarro-Hortal, María D. A1 - Robles-Almazán, María A1 - Bullón, Beatriz A1 - Cordero, Mario D. A1 - Battino, Maurizio A1 - Quiles, José L. Y1 - 2020/// ER - TY - JOUR IS - 4 ID - uneatlantico81 TI - Autophagy in Human Health and Disease: Novel Therapeutic Opportunities AV - none UR - http://doi.org/10.1089/ars.2017.7234 KW - Autophagy; Natural bioactive compounds; Disease prevention; Molecular target; Epignetic modifcation. Y1 - 2019/00/00/ A1 - Giampieri, Francesca A1 - Afrin, Sadia A1 - Forbes-Hernandez, Tamara Y. A1 - Gasparrini, Massimiliano A1 - Cianciosi, Danila A1 - Reboredo-Rodriguez, Patricia A1 - Varela-Lopez, Alfonso A1 - Quiles, Jose L. A1 - Battino, Maurizio JF - Antioxidants & Redox Signaling SN - 1523-0864 N2 - Significance: In eukaryotes, autophagy represents a highly evolutionary conserved process, through which macromolecules and cytoplasmic material are degraded into lysosomes and recycled for biosynthetic or energetic purposes. Dysfunction of the autophagic process has been associated with the onset and development of many human chronic pathologies, such as cardiovascular, metabolic, and neurodegenerative diseases as well as cancer. Recent Advances: Currently, comprehensive research is being carried out to discover new therapeutic agents that are able to modulate the autophagic process in vivo. Recent evidence has shown that a large number of natural bioactive compounds are involved in the regulation of autophagy by modulating several transcriptional factors and signaling pathways. Critical Issues: Critical issues that deserve particular attention are the inadequate understanding of the complex role of autophagy in disease pathogenesis, the limited availability of therapeutic drugs, and the lack of clinical trials. In this context, the effects that natural bioactive compounds exert on autophagic modulation should be clearly highlighted, since they depend on the type and stage of the pathological conditions of diseases. Future Directions: Research efforts should now focus on understanding the survival-supporting and death-promoting roles of autophagy, how natural compounds interact exactly with the autophagic targets so as to induce or inhibit autophagy and on the evaluation of their pharmacological effects in a more in-depth and mechanistic way. In addition, clinical studies on autophagy-inducing natural products are strongly encouraged, also to highlight some fundamental aspects, such as the dose, the duration, and the possible synergistic action of these compounds with conventional therapy. EP - 634 VL - 30 SP - 577 ER - TY - JOUR UR - http://doi.org/10.1016/j.lwt.2017.08.079 AV - none TI - Apis mellifera vs Melipona beecheii Cuban polifloral honeys: A comparison based on their physicochemical parameters, chemical composition and biological properties ID - uneatlantico82 KW - Honey; Polyphenols; Mollipona beecherii; Total antioxidant capacity; Antimicrobial activity. A1 - Alvarez-Suarez, José M. A1 - Giampieri, Francesca A1 - Brenciani, Andrea A1 - Mazzoni, Luca A1 - Gasparrini, Massimiliano A1 - González-Paramás, Ana M. A1 - Santos-Buelga, Celestino A1 - Morroni, Gianluca A1 - Simoni, Serena A1 - Forbes-Hernández, Tamara Y. A1 - Afrin, Sadia A1 - Giovanetti, Eleonora A1 - Battino, Maurizio Y1 - 2018/00/00/ SP - 272 EP - 279 N2 - Several polifloral honeys from the Apis mellifera and Melipona beecheii bee were analyzed to evaluate their pollen profile, physicochemical parameters, chemical composition and their antioxidant and antimicrobial activity. Flavonoids and other phenolic derivatives, 16 compounds in Apis mellifera honeys and 19 compounds in Melipona beecheii honeys, were identified using the HPLC-DAD-ESI-MS/MS analysis. The physicochemical parameters were within the ranges reported for these honeys, showing some of the distinctive characteristics of M. beecheii honeys compared to A. mellifera honeys, such as their high moisture and acidity. M. beecheii honey showed the highest values of total antioxidant capacity and total phenolic, flavonoid, carotenoids, ascorbic acid, free amino acid and protein contents compared to A. mellifera honey. M. beecheii honey also exhibited a higher antimicrobial activity. Our result shows that M. beecheii honeys is an important source of bioactive compounds with relevant biological properties compared to A. mellifera honey. VL - 87 JF - LWT SN - 0023-6438 ER - TY - JOUR JF - Plant Foods for Human Nutrition SN - 0921-9668 EP - 24 N2 - We analyzed guava fruits (Psidium guajava L. cv. Red Suprema) from Cuba to determine their chemical composition, total antioxidant capacity, as well as their protective effect against oxidative damage using an in vitro model of human dermal fibroblasts. The guava fruit is a natural source of bioactive compounds, such as polyphenols, vitamin C, folates and beta carotenes with proven health benefits. Human dermal fibroblasts were pre-incubated with different concentrations of guava crude extract and then subjected to oxidative stress using the AAPH stressor. The number of apoptotic and dead cells, as well as the markers of oxidative damage such as lipid and protein oxidation significantly decreased when cells were pre-incubated with guava crude extract and then exposed to the stressor. The activity of antioxidant enzymes also improved when cells were pre-incubated with guava crude extract in comparison to cells subjected to stress without prior pre-incubation with the guava extract. The results obtained in this study highlight the health benefits of guava regarding oxidative stress, proving it to be an important source of bioactive compounds associated with important biological properties. VL - 73 SP - 18 Y1 - 2018/00/00/ A1 - Alvarez-Suarez, José M. A1 - Giampieri, Francesca A1 - Gasparrini, Massimiliano A1 - Mazzoni, Luca A1 - Forbes-Hernández, Tamara Y. A1 - Afrin, Sadia A1 - Battino, Maurizio KW - Guava fruit; Antioxidant capacity; Human dermal fibroblast; Oxidative stress. IS - 1 TI - Guava (Psidium guajava L. cv. Red Suprema) Crude Extract Protect Human Dermal Fibroblasts against Cytotoxic Damage Mediated by Oxidative Stress ID - uneatlantico83 AV - none UR - http://doi.org/10.1007/s11130-018-0657-2 ER - TY - JOUR SN - 0734-9750 JF - Biotechnology Advances SP - 358 N2 - Organisms are constantly exposed to a broad range of pathological and stress-inducing agents, allergens and environmental chemicals that can induce infections, toxicity or other undesirable reactions. Our immune system has evolved over time in order to efficiently respond to these exogenous insults and maintain homeostasis. In particular, the innate immune system acts as primary barrier to prevent the entrance of invasive agents or allergens. This system is comprised of a diversity of cell types that are rapidly activated by recognition of common structures present in many potential pathogens known as pathogen-associated molecular patterns (PAMPs). The nuclear factor erythroid 2 related factor 2 (Nrf2) is a relevant basic leucine zipper (bZIP) transcription factor that is essential in the regulation of cell cycle homeostasis, cytoprotection, and innate immunity when cells are under stressful conditions. Although the role of Nrf2 in activating the expression of protective genes ? such as antioxidant or anti-inflammatory ? is known, its role in innate immunity and immune-related gene expression remains not yet clear. The present review summarizes current knowledge on Nrf2 signaling pathway structure and activity under both physiological state and upon oxidative stress. In addition, the relation between Nrf2 signaling pathway and the innate immune system is discussed, highlighting the potential therapeutic effects of diverse natural and synthetic compounds as Nrf2 regulators. VL - 36 EP - 370 Y1 - 2018/00/00/ A1 - Battino, Maurizio A1 - Giampieri, Francesca A1 - Pistollato, Francesca A1 - Sureda, Antoni A1 - de Oliveira, Marcos Roberto A1 - Pittalà, Valeria A1 - Fallarino, Francesca A1 - Nabavi, Seyed Fazel A1 - Atanasov, Atanas Georgiev A1 - Nabavi, Seyed Mohammad KW - Immune system; Inflammation; Natural compounds; Nuclear factor; Oxidative stress. TI - Nrf2 as regulator of innate immunity: A molecular Swiss army knife! ID - uneatlantico84 IS - 2 UR - http://doi.org/10.1016/j.biotechadv.2017.12.012 AV - none ER - TY - JOUR TI - Intracellular signaling pathways of inflammation modulated by dietary flavonoids: The most recent evidence ID - uneatlantico85 IS - 17 UR - http://doi.org/10.1080/10408398.2017.1345853 AV - none KW - Flavonoids; Inflammation; Intracellular signaling pathways; Strectyre-activity relationship. Y1 - 2018/00/00/ A1 - Chen, Lei A1 - Teng, Hui A1 - Jia, Zhen A1 - Battino, Maurizio A1 - Miron, Anca A1 - Yu, Zhiling A1 - Cao, Hui A1 - Xiao, Jianbo SN - 1040-8398 JF - Critical Reviews in Food Science and Nutrition SP - 2908 EP - 2924 N2 - Dietary flavonoids, which occur in many plant foods, are considered as the most active constituents among the plant-derived ones in vitro and in vivo. To date, many studies have addressed the anti-inflammatory activity of flavonoids. However, their considerable structural diversity and in vivo bioavailability make them able to modulate different signaling pathways. The present review attempted to summarize and highlight a broad range of inflammation-associated signaling pathways modulated by flavonoids. Finally, based on the current scientist's literature, structure-activity relationships were concluded. Dietary flavonoids have the ability to attenuate inflammation by targeting different intracellular signaling pathways triggered by NF-?B, AP-1, PPAR, Nrf2, and MAPKs. Identification of the main structural features required for the modulation of these inflammation-related pathways (hydroxylation pattern, C2=C3 double bond) have an important role to play in the development of new anti-inflammatory drugs. VL - 58 ER - TY - JOUR KW - Honey; Antioxidants; Polyphenols; Antimicrobial activities; Cancer; Diabetes; Disease prevention. AV - public UR - http://doi.org/10.3390/molecules23092322 IS - 9 TI - Phenolic Compounds in Honey and Their Associated Health Benefits: A Review ID - uneatlantico86 VL - 23 N2 - Honey is a natural substance appreciated for its therapeutic abilities since ancient times. Its content in flavonoids and phenolic acids plays a key role on human health, thanks to the high antioxidant and anti-inflammatory properties that they exert. Honey possesses antimicrobial capacity and anticancer activity against different types of tumors, acting on different molecular pathways that are involved on cellular proliferation. In addition, an antidiabetic activity has also been highlighted, with the reduction of glucose, fructosamine, and glycosylated hemoglobin serum concentration. Honey exerts also a protective effect in the cardiovascular system, where it mainly prevents the oxidation of low-density lipoproteins, in the nervous system, in the respiratory system against asthma and bacterial infections, and in the gastrointestinal system. A beneficial effect of honey can also be demonstrated in athletes. The purpose of this review is to summarize and update the current information regarding the role of honey in health and diseases. SN - 1420-3049 JF - Molecules A1 - Cianciosi, Danila A1 - Forbes-Hernández, Tamara Y. A1 - Afrin, Sadia A1 - Gasparrini, Massimiliano A1 - Reboredo-Rodriguez, Patricia A1 - Manna, Piera A1 - Zhang, Jiaojiao A1 - Bravo Lamas, Leire A1 - Martínez Flórez, Susana A1 - Agudo Toyos, Pablo A1 - Quiles, José A1 - Giampieri, Francesca A1 - Battino, Maurizio Y1 - 2018/00/00/ ER - TY - JOUR ID - uneatlantico129 TI - Strawberry extracts efficiently counteract inflammatory stress induced by the endotoxin lipopolysaccharide in Human Dermal Fibroblast UR - http://doi.org/10.1016/j.fct.2018.02.038 AV - none KW - Strawberry; LPS; Human Dermal Fibroblast; Inflammation; Oxidative stress; Antioxidant response; Prevention. Y1 - 2018/// A1 - Gasparrini, Massimiliano A1 - Giampieri, Francesca A1 - Forbes-Hernandez, Tamara Y. A1 - Afrin, Sadia A1 - Cianciosi, Danila A1 - Reboredo-Rodriguez, Patricia A1 - Varela-Lopez, Alfonso A1 - Zhang, JiaoJiao A1 - Quiles, Josè L. A1 - Mezzetti, Bruno A1 - Bompadre, Stefano A1 - Battino, Maurizio JF - Food and Chemical Toxicology SN - 0278-6915 SP - 128 N2 - A protracted pro-inflammatory state is the common denominator in the development, progression and complication of the common chronic diseases. Dietary antioxidants represent an efficient tool to counteract this inflammatory state. The aim of the present work was to evaluate the effects of strawberry extracts on inflammation evoked by E. Coli lipopolysaccharide in Human Dermal Fibroblast, by measuring reactive oxygen species production, apoptosis rate, antioxidant enzymes activity, mitochondria functionality and also investigating the molecular pathway involved in inflammatory and antioxidant response. The results demonstrated that strawberry pre-treatment reduced intracellular reactive oxygen species levels, apoptotic rate, improved antioxidant defences and mitochondria functionality in lipopolysaccharide -treated cells. Strawberry exerted these protective activities through the inhibition of the NF-kB signalling pathway and the stimulation of the Nrf2 pathway, with a mechanism AMPK-dependent. These results confirm the health benefits of strawberry in the prevention of inflammation and oxidative stress condition in lipopolysaccharide-treated cells. EP - 140 VL - 114 ER - TY - JOUR Y1 - 2018/// A1 - Giampieri, Francesca A1 - Afrin, Sadia A1 - Stewart, Derek A1 - McDougall, Gordon A1 - Brennan, Rex A1 - Blyth, Lesley A1 - Gasparrini, Massimiliano A1 - Mazzoni, Luca A1 - Capocasa, Franco A1 - Alvarez-Suarez, José M. A1 - Bompadre, Stefano A1 - Nogueira Brás de Oliveira, Pedro A1 - N. Santos, Claudia A1 - Masias Vergara, Manuel A1 - Agudo Toyos, Pablo A1 - Crespo-Álvarez, Jorge A1 - Mezzetti, Bruno A1 - Forbes-Hernandez, Tamara Y. A1 - Battino, Maurizio SN - 1420-3049 JF - Molecules N2 - Berry fruits are rich in nutrients and polyphenols, providing potential health benefits. Understanding the factors that affect their bioavailability is becoming of utmost importance for evaluating their biological significance and efficacy as functional food. In this study, the phytochemical composition and the total antioxidant capacity of different varieties of five berries (blackberry, blackcurrant, blueberry, raspberry, and strawberry) were evaluated after an in vitro gastrointestinal digestion process. The cultivar of each berry that showed the higher content of total phenols and flavonoids was selected to study its cytotoxic effect on human hepatoma cells. Digestion resulted in a high reduction (p ? 0.05) of total phenolic, flavonoid and anthocyanin contents and total antioxidant capacity, in the ?IN? samples compared to the ?OUT? extracts, which represent the ?serum-available? and the ?colon-available? fractions, respectively. Incubation of the digested fraction for 24 h didn?t exert any effect on cellular viability, while a dose- and time-dependent cytotoxicity was observed after 48 h and 72 h of incubation for all the berries analyzed. Our results suggest that the approach proposed in this work may represent a rapid tool for evaluating and identifying new berries with increased phytochemical bioavailability, highlighting their antiproliferative agents after an in vitro digestion. VL - 23 IS - 8 TI - Phytochemical Composition and Cytotoxic Effects on Liver Hepatocellular Carcinoma Cells of Different Berries Following a Simulated In Vitro Gastrointestinal Digestion ID - uneatlantico130 AV - public UR - http://doi.org/10.3390/molecules23081918 KW - Berry; Polyphenols; In vitro gastrointestinal digestion; Bioavailability; Cytotoxicity. ER - TY - JOUR VL - 66 N2 - Food fortification through the increase and/or modulation of bioactive compounds has become a major goal for preventing several diseases, including cancer. Here, strawberry lines of cv. Calypso transformed with a construct containing an anthocyanidin synthase (ANS) gene were produced to study the effects on anthocyanin biosynthesis, metabolism, and transcriptome. Three strawberry ANS transgenic lines (ANS L5, ANS L15, and ANS L18) were analyzed for phytochemical composition and total antioxidant capacity (TAC), and their fruit extracts were assessed for cytotoxic effects on hepatocellular carcinoma. ANS L18 fruits had the highest levels of total phenolics and flavonoids, while those of ANS L15 had the highest anthocyanin concentration; TAC positively correlated with total polyphenol content. Fruit transcriptome was also specifically affected in the polyphenol biosynthesis and in other related metabolic pathways. Fruit extracts of all lines exerted cytotoxic effects in a dose/time-dependent manner, increasing cellular apoptosis and free radical levels and impairing mitochondrial functionality. EP - 592 SP - 581 JF - Journal of Agricultural and Food Chemistry SN - 0021-8561 A1 - Giampieri, Francesca A1 - Gasparrini, Massimiliano A1 - Forbes-Hernandez, Tamara Y. A1 - Mazzoni, Luca A1 - Capocasa, Franco A1 - Sabbadini, Silvia A1 - Alvarez-Suarez, José M. A1 - Afrin, Sadia A1 - Rosati, Carlo A1 - Pandolfini, Tiziana A1 - Molesini, Barbara A1 - Sánchez-Sevilla, José Federico A1 - Amaya, Iraida A1 - Mezzetti, Bruno A1 - Battino, Maurizio Y1 - 2018/// KW - Anthocyanidin synthase; Anticancer effects; Apoptosis; Flavonoids; RNA-seq. AV - none UR - http://doi.org/10.1021/acs.jafc.7b04177 IS - 3 TI - Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells ID - uneatlantico87 ER - TY - JOUR Y1 - 2018/// A1 - Giampieri, Francesca A1 - Quiles, José L. A1 - Orantes-Bermejo, Francisco J. A1 - Gasparrini, Massimiliano A1 - Forbes-Hernandez, Tamara Y. A1 - Sánchez-González, Cristina A1 - Llopis, Juan A1 - Rivas-García, Lorenzo A1 - Afrin, Sadia A1 - Varela-López, Alfonso A1 - Cianciosi, Danila A1 - Reboredo-Rodriguez, Patricia A1 - Fernández-Piñar, Cristina Torres A1 - Caderón Iglesia, Rubén A1 - Ruiz Salces, Roberto A1 - Aparicio Obregón, Silvia A1 - Crespo-Álvarez, Jorge A1 - Dzul Lopez, Luis A1 - Xiao, Jianbo A1 - Battino, Maurizio SN - 0278-6915 JF - Food and Chemical Toxicology N2 - During the process of beeswax recycling, many industrial derivatives are obtained. These matrices may have an interesting healthy and commercial potential but to date they have not been properly studied. The aim of the present work was to evaluate the proximal and phytochemical composition, the antioxidant capacity and cytotoxic effects of two by-products from beeswax recycling process named MUD 1 and MUD 2 on liver hepatocellular carcinoma. Our results showed that MUD 1 presented the highest (P?