relation: http://repositorio.uneatlantico.es/id/eprint/77/ canonical: http://repositorio.uneatlantico.es/id/eprint/77/ title: Nutritional Value and Preventive Role of Nigella sativa L. and Its Main Component Thymoquinone in Cancer: An Evidenced-Based Review of Preclinical and Clinical Studies creator: Ansary, Johura creator: Giampieri, Francesca creator: Forbes-Hernandez, Tamara Y. creator: Regolo, Lucia creator: Quinzi, Denise creator: Gracia Villar, Santos creator: Garcia Villena, Eduardo creator: Tutusaus, Kilian creator: Alvarez-Suarez, José M. creator: Battino, Maurizio creator: Cianciosi, Danila subject: Alimentación description: In recent times, scientific attention has been paid to different foods and their bioactive components for the ability to inhibit the onset and progress of different types of cancer. Nigella sativa extract, powder and seed oil and its main components, thymoquinone and α-hederin, have showed potent anticancer and chemosensitizing effects against various types of cancer, such as liver, colon, breast, renal, cervical, lung, ovarian, pancreatic, prostate and skin tumors, through the modulation of various molecular signaling pathways. Herein, the purpose of this review was to highlight the anticancer activity of Nigella sativa and it constitutes, focusing on different in vitro, in vivo and clinical studies and projects, in order to underline their antiproliferative, proapoptotic, cytotoxic and antimetastatic effects. Particular attention has been also given to the synergistic effect of Nigella sativa and it constitutes with chemotherapeutic drugs, and to the synthesized analogs of thymoquinone that seem to enhance the chemo-sensitizing potential. This review could be a useful step towards new research on N. sativa and cancer, to include this plant in the dietary treatments in support to conventional therapies, for the best achievement of therapeutic goals. date: 2021-04 type: Artículo type: PeerReviewed format: text language: en rights: cc_by_sa_4 identifier: http://repositorio.uneatlantico.es/id/eprint/77/1/molecules-26-02108-v2.pdf identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica Abierto Inglés In recent times, scientific attention has been paid to different foods and their bioactive components for the ability to inhibit the onset and progress of different types of cancer. Nigella sativa extract, powder and seed oil and its main components, thymoquinone and α-hederin, have showed potent anticancer and chemosensitizing effects against various types of cancer, such as liver, colon, breast, renal, cervical, lung, ovarian, pancreatic, prostate and skin tumors, through the modulation of various molecular signaling pathways. Herein, the purpose of this review was to highlight the anticancer activity of Nigella sativa and it constitutes, focusing on different in vitro, in vivo and clinical studies and projects, in order to underline their antiproliferative, proapoptotic, cytotoxic and antimetastatic effects. Particular attention has been also given to the synergistic effect of Nigella sativa and it constitutes with chemotherapeutic drugs, and to the synthesized analogs of thymoquinone that seem to enhance the chemo-sensitizing potential. This review could be a useful step towards new research on N. sativa and cancer, to include this plant in the dietary treatments in support to conventional therapies, for the best achievement of therapeutic goals. metadata Ansary, Johura; Giampieri, Francesca; Forbes-Hernandez, Tamara Y.; Regolo, Lucia; Quinzi, Denise; Gracia Villar, Santos; Garcia Villena, Eduardo; Tutusaus, Kilian; Alvarez-Suarez, José M.; Battino, Maurizio y Cianciosi, Danila mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, santos.gracia@uneatlantico.es, eduardo.garcia@uneatlantico.es, kilian.tutusaus@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2021) Nutritional Value and Preventive Role of Nigella sativa L. and Its Main Component Thymoquinone in Cancer: An Evidenced-Based Review of Preclinical and Clinical Studies. Molecules, 26 (8). p. 2108. ISSN 1420-3049 relation: http://doi.org/10.3390/molecules26082108 relation: doi:10.3390/molecules26082108 language: en relation: http://repositorio.uneatlantico.es/id/eprint/104/ canonical: http://repositorio.uneatlantico.es/id/eprint/104/ title: Potential Role of the Mitochondria for the Dermatological Treatment of Papillon-Lefèvre creator: Castejón-Vega, Beatriz creator: Battino, Maurizio creator: Quiles, José L. creator: Bullon, Beatriz creator: Cordero, Mario D. creator: Bullón, Pedro subject: Biomedicina description: The Papillon–Lefèvre syndrome (PLS) is a rare autosomal recessive disorder caused by mutations in the Cathepsin C (CTSC) gene, characterized by periodontitis and palmoplantar hyperkeratosis. The main inflammatory deficiencies include oxidative stress and autophagic dysfunction. Mitochondria are the main source of reactive oxygen species; their impaired function is related to skin diseases and periodontitis. The mitochondrial function has been evaluated in PLS and mitochondria have been targeted as a possible treatment for PLS. We show for the first time an important mitochondrial dysfunction associated with increased oxidative damage of mtDNA, reduced CoQ10 and mitochondrial mass and aberrant morphologies of the mitochondria in PLS patients. Mitochondrial dysfunction, determined by oxygen consumption rate (OCR) in PLS fibroblasts, was treated with CoQ10 supplementation, which determined an improvement in OCR and a remission of skin damage in a patient receiving a topical administration of a cream enriched with CoQ10 0.1%. We provide the first evidence of the role of mitochondrial dysfunction and CoQ10 deficiency in the pathophysiology of PLS and a future therapeutic option for PLS. date: 2021-01 type: Artículo type: PeerReviewed format: text language: en rights: cc_by_4 identifier: http://repositorio.uneatlantico.es/id/eprint/104/1/antioxidants-10-00095-v2.pdf identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Abierto Inglés The Papillon–Lefèvre syndrome (PLS) is a rare autosomal recessive disorder caused by mutations in the Cathepsin C (CTSC) gene, characterized by periodontitis and palmoplantar hyperkeratosis. The main inflammatory deficiencies include oxidative stress and autophagic dysfunction. Mitochondria are the main source of reactive oxygen species; their impaired function is related to skin diseases and periodontitis. The mitochondrial function has been evaluated in PLS and mitochondria have been targeted as a possible treatment for PLS. We show for the first time an important mitochondrial dysfunction associated with increased oxidative damage of mtDNA, reduced CoQ10 and mitochondrial mass and aberrant morphologies of the mitochondria in PLS patients. Mitochondrial dysfunction, determined by oxygen consumption rate (OCR) in PLS fibroblasts, was treated with CoQ10 supplementation, which determined an improvement in OCR and a remission of skin damage in a patient receiving a topical administration of a cream enriched with CoQ10 0.1%. We provide the first evidence of the role of mitochondrial dysfunction and CoQ10 deficiency in the pathophysiology of PLS and a future therapeutic option for PLS. metadata Castejón-Vega, Beatriz; Battino, Maurizio; Quiles, José L.; Bullon, Beatriz; Cordero, Mario D. y Bullón, Pedro mail SIN ESPECIFICAR, SIN ESPECIFICAR, jose.quiles@uneatlantico.es, SIN ESPECIFICAR, jose.quiles@uneatlantico.es, SIN ESPECIFICAR (2021) Potential Role of the Mitochondria for the Dermatological Treatment of Papillon-Lefèvre. Antioxidants, 10 (1). p. 95. ISSN 2076-3921 relation: http://doi.org/10.3390/antiox10010095 relation: doi:10.3390/antiox10010095 language: en relation: http://repositorio.uneatlantico.es/id/eprint/105/ canonical: http://repositorio.uneatlantico.es/id/eprint/105/ title: The Molecular Basis of Different Approaches for the Study of Cancer Stem Cells and the Advantages and Disadvantages of a Three-Dimensional Culture creator: Cianciosi, Danila creator: Ansary, Johura creator: Forbes-Hernandez, Tamara Y. creator: Regolo, Lucia creator: Quinzi, Denise creator: Gracia Villar, Santos creator: Garcia Villena, Eduardo creator: Tutusaus Pifarre, Kilian creator: Alvarez-Suarez, José M. creator: Battino, Maurizio creator: Giampieri, Francesca subject: Biomedicina description: Cancer stem cells (CSCs) are a rare tumor subpopulation with high differentiation, proliferative and tumorigenic potential compared to the remaining tumor population. CSCs were first discovered by Bonnet and Dick in 1997 in acute myeloid leukemia. The identification and isolation of these cells in this pioneering study were carried out through the flow cytometry, exploiting the presence of specific cell surface molecular markers (CD34+/CD38−). In the following years, different strategies and projects have been developed for the study of CSCs, which are basically divided into surface markers assays and functional assays; some of these techniques also allow working with a cellular model that better mimics the tumor architecture. The purpose of this mini review is to summarize and briefly describe all the current methods used for the identification, isolation and enrichment of CSCs, describing, where possible, the molecular basis, the advantages and disadvantages of each technique with a particular focus on those that offer a three-dimensional culture. date: 2021-04 type: Artículo type: PeerReviewed format: text language: en rights: cc_by_4 identifier: http://repositorio.uneatlantico.es/id/eprint/105/1/molecules-26-02615-v4.pdf identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica Abierto Inglés Cancer stem cells (CSCs) are a rare tumor subpopulation with high differentiation, proliferative and tumorigenic potential compared to the remaining tumor population. CSCs were first discovered by Bonnet and Dick in 1997 in acute myeloid leukemia. The identification and isolation of these cells in this pioneering study were carried out through the flow cytometry, exploiting the presence of specific cell surface molecular markers (CD34+/CD38−). In the following years, different strategies and projects have been developed for the study of CSCs, which are basically divided into surface markers assays and functional assays; some of these techniques also allow working with a cellular model that better mimics the tumor architecture. The purpose of this mini review is to summarize and briefly describe all the current methods used for the identification, isolation and enrichment of CSCs, describing, where possible, the molecular basis, the advantages and disadvantages of each technique with a particular focus on those that offer a three-dimensional culture. metadata Cianciosi, Danila; Ansary, Johura; Forbes-Hernandez, Tamara Y.; Regolo, Lucia; Quinzi, Denise; Gracia Villar, Santos; Garcia Villena, Eduardo; Tutusaus Pifarre, Kilian; Alvarez-Suarez, José M.; Battino, Maurizio y Giampieri, Francesca mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, santos.gracia@uneatlantico.es, eduardo.garcia@uneatlantico.es, kilian.tutusaus@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2021) The Molecular Basis of Different Approaches for the Study of Cancer Stem Cells and the Advantages and Disadvantages of a Three-Dimensional Culture. Molecules, 26 (9). p. 2615. ISSN 1420-3049 relation: http://doi.org/10.3390/molecules26092615 relation: doi:10.3390/molecules26092615 language: en relation: http://repositorio.uneatlantico.es/id/eprint/108/ canonical: http://repositorio.uneatlantico.es/id/eprint/108/ title: Rosa x hybrida extracts with dual actions: Antiproliferative effects against tumour cells and inhibitor of Alzheimer disease creator: Rivas-García, Lorenzo creator: Quiles, José L. creator: Roma-Rodrigues, Catarina creator: Raposo, Luis R. creator: Navarro-Hortal, María D. creator: Romero-Márquez, Jose M. creator: Esteban-Muñoz, Adelaida creator: Varela-López, Alfonso creator: García, Laura Carrera creator: Cianciosi, Danila creator: Forbes Hernandez, Tamara Y. creator: Battino, Maurizio creator: Llopis, Juan creator: Fernandes, Alexandra R. creator: Baptista, Pedro V. creator: Sánchez-González, Cristina subject: Biomedicina description: Edible flowers are being used as a new ingredient in modern gastronomy. Recently, these products have also gained interest as an important source of phenolic compounds with potential for biomedical applications. The present work studied a methanolic extract of Rosa x hybrida in which 35 individual phenolic compounds were identified. The extract has been evaluated for its antiproliferative properties in ovarian carcinoma cells. Results showed that the antiproliferative effect was associated with the induction of autophagy and apoptosis with the concomitant ROS increase probably related to mitochondria dysfunction. These antiproliferative effects might be associated with some components of the extract such as quercetin. The extract did not induce damage in healthy cells and that it was able to improve the wound healing activity. The present study also evaluated the properties of the mentioned extract in vivo in C. elegans. Tests demonstrated a lack of toxicity in the worm model. Promising results have been obtained in transgenic strains of C. elegans that produce human beta amyloid peptide, suggesting the possible utility of the extract from the point of view of Alzheimer disease. Altogether, results suggest that Rosa x hybrida extracts could be a new tool for the development of functional foods. date: 2021 type: Artículo type: PeerReviewed identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Cerrado Inglés Edible flowers are being used as a new ingredient in modern gastronomy. Recently, these products have also gained interest as an important source of phenolic compounds with potential for biomedical applications. The present work studied a methanolic extract of Rosa x hybrida in which 35 individual phenolic compounds were identified. The extract has been evaluated for its antiproliferative properties in ovarian carcinoma cells. Results showed that the antiproliferative effect was associated with the induction of autophagy and apoptosis with the concomitant ROS increase probably related to mitochondria dysfunction. These antiproliferative effects might be associated with some components of the extract such as quercetin. The extract did not induce damage in healthy cells and that it was able to improve the wound healing activity. The present study also evaluated the properties of the mentioned extract in vivo in C. elegans. Tests demonstrated a lack of toxicity in the worm model. Promising results have been obtained in transgenic strains of C. elegans that produce human beta amyloid peptide, suggesting the possible utility of the extract from the point of view of Alzheimer disease. Altogether, results suggest that Rosa x hybrida extracts could be a new tool for the development of functional foods. metadata Rivas-García, Lorenzo; Quiles, José L.; Roma-Rodrigues, Catarina; Raposo, Luis R.; Navarro-Hortal, María D.; Romero-Márquez, Jose M.; Esteban-Muñoz, Adelaida; Varela-López, Alfonso; García, Laura Carrera; Cianciosi, Danila; Forbes Hernandez, Tamara Y.; Battino, Maurizio; Llopis, Juan; Fernandes, Alexandra R.; Baptista, Pedro V. y Sánchez-González, Cristina mail SIN ESPECIFICAR, jose.quiles@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2021) Rosa x hybrida extracts with dual actions: Antiproliferative effects against tumour cells and inhibitor of Alzheimer disease. Food and Chemical Toxicology, 149. p. 112018. ISSN 0278-6915 relation: http://doi.org/10.1016/j.fct.2021.112018 relation: doi:10.1016/j.fct.2021.112018 language: en relation: http://repositorio.uneatlantico.es/id/eprint/109/ canonical: http://repositorio.uneatlantico.es/id/eprint/109/ title: Ultra-Small Iron Nanoparticles Target Mitochondria Inducing Autophagy, Acting on Mitochondrial DNA and Reducing Respiration creator: Rivas-García, Lorenzo creator: Quiles, José L. creator: Varela-López, Alfonso creator: Giampieri, Francesca creator: Battino, Maurizio creator: Bettmer, Jörg creator: Montes-Bayón, María creator: Llopis, Juan creator: Sánchez-González, Cristina subject: Biomedicina description: The application of metallic nanoparticles (materials with size at least in one dimension ranging from 1 to 100 nm) as a new therapeutic tool will improve the diagnosis and treatment of diseases. The mitochondria could be a therapeutic target to treat pathologies whose origin lies in mitochondrial dysfunctions or whose progression is dependent on mitochondrial function. We aimed to study the subcellular distribution of 2-4 nm iron nanoparticles and its effect on mitochondrial DNA (mtDNA), mitochondrial function, and autophagy in colorectal cell lines (HT-29). Results showed that when cells were exposed to ultra-small iron nanoparticles, their subcellular fate was mainly mitochondria, affecting its respiratory and glycolytic parameters, inducing the migration of the cellular state towards quiescence, and promoting and triggering the autophagic process. These effects support the potential use of nanoparticles as therapeutic agents using mitochondria as a target for cancer and other treatments for mitochondria-dependent pathologies. date: 2021-01 type: Artículo type: PeerReviewed format: text language: en rights: cc_by_4 identifier: http://repositorio.uneatlantico.es/id/eprint/109/1/pharmaceutics-13-00090.pdf identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Abierto Inglés The application of metallic nanoparticles (materials with size at least in one dimension ranging from 1 to 100 nm) as a new therapeutic tool will improve the diagnosis and treatment of diseases. The mitochondria could be a therapeutic target to treat pathologies whose origin lies in mitochondrial dysfunctions or whose progression is dependent on mitochondrial function. We aimed to study the subcellular distribution of 2-4 nm iron nanoparticles and its effect on mitochondrial DNA (mtDNA), mitochondrial function, and autophagy in colorectal cell lines (HT-29). Results showed that when cells were exposed to ultra-small iron nanoparticles, their subcellular fate was mainly mitochondria, affecting its respiratory and glycolytic parameters, inducing the migration of the cellular state towards quiescence, and promoting and triggering the autophagic process. These effects support the potential use of nanoparticles as therapeutic agents using mitochondria as a target for cancer and other treatments for mitochondria-dependent pathologies. metadata Rivas-García, Lorenzo; Quiles, José L.; Varela-López, Alfonso; Giampieri, Francesca; Battino, Maurizio; Bettmer, Jörg; Montes-Bayón, María; Llopis, Juan y Sánchez-González, Cristina mail SIN ESPECIFICAR, jose.quiles@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2021) Ultra-Small Iron Nanoparticles Target Mitochondria Inducing Autophagy, Acting on Mitochondrial DNA and Reducing Respiration. Pharmaceutics, 13 (1). p. 90. ISSN 1999-4923 relation: http://doi.org/10.3390/pharmaceutics13010090 relation: doi:10.3390/pharmaceutics13010090 language: en relation: http://repositorio.uneatlantico.es/id/eprint/110/ canonical: http://repositorio.uneatlantico.es/id/eprint/110/ title: The central role of mitochondria in the relationship between dietary lipids and cancer progression creator: Varela-López, Alfonso creator: Vera-Ramírez, Laura creator: Giampieri, Francesca creator: Navarro-Hortal, María D. creator: Forbes-Hernandez, Tamara Y. creator: Battino, Maurizio creator: Quiles, José L. subject: Biomedicina description: Evidence demonstrates the importance of lipid metabolism and signaling in cancer cell biology. De novo lipogenesis is an important source of lipids for cancer cells, but exogenous lipid uptake remains essential for many cancer cells. Dietary lipids can modify lipids present in tumor microenvironment affecting cancer cell metabolism. Clinical trials have shown that diets rich in polyunsaturated fatty acids (PUFA) can negatively affect tumor growth. However, certain n-6 PUFAs can also contribute to cancer progression. Identifying the molecular mechanisms through which lipids affect cancer progression will provide an opportunity for focused dietary interventions that could translate into the development of personalized diets for cancer control. However, the effective mechanisms of action of PUFAs have not been fully clarified yet. Mitochondria controls ATP generation, redox homeostasis, metabolic signaling, apoptotic pathways and many aspects of autophagy, and it has been recognized to play a key role in cancer. The purpose of this review is to summarize the current evidence linking dietary lipids effects on mitochondrial aspects with consequences for cancer progression and the molecular mechanisms that underlie this association. date: 2021-08 type: Artículo type: PeerReviewed identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Cerrado Inglés Evidence demonstrates the importance of lipid metabolism and signaling in cancer cell biology. De novo lipogenesis is an important source of lipids for cancer cells, but exogenous lipid uptake remains essential for many cancer cells. Dietary lipids can modify lipids present in tumor microenvironment affecting cancer cell metabolism. Clinical trials have shown that diets rich in polyunsaturated fatty acids (PUFA) can negatively affect tumor growth. However, certain n-6 PUFAs can also contribute to cancer progression. Identifying the molecular mechanisms through which lipids affect cancer progression will provide an opportunity for focused dietary interventions that could translate into the development of personalized diets for cancer control. However, the effective mechanisms of action of PUFAs have not been fully clarified yet. Mitochondria controls ATP generation, redox homeostasis, metabolic signaling, apoptotic pathways and many aspects of autophagy, and it has been recognized to play a key role in cancer. The purpose of this review is to summarize the current evidence linking dietary lipids effects on mitochondrial aspects with consequences for cancer progression and the molecular mechanisms that underlie this association. metadata Varela-López, Alfonso; Vera-Ramírez, Laura; Giampieri, Francesca; Navarro-Hortal, María D.; Forbes-Hernandez, Tamara Y.; Battino, Maurizio y Quiles, José L. mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, jose.quiles@uneatlantico.es (2021) The central role of mitochondria in the relationship between dietary lipids and cancer progression. Seminars in Cancer Biology. ISSN 1044579X relation: http://doi.org/10.1016/j.semcancer.2021.01.001 relation: doi:10.1016/j.semcancer.2021.01.001 language: en relation: http://repositorio.uneatlantico.es/id/eprint/78/ canonical: http://repositorio.uneatlantico.es/id/eprint/78/ title: Links between Nutrition, Infectious Diseases, and Microbiota: Emerging Technologies and Opportunities for Human-Focused Research creator: Cassotta, Manuela creator: Forbes-Hernández, Tamara Y. creator: Calderón Iglesias, Ruben creator: Ruiz, Roberto creator: Elexpuru Zabaleta, Maria creator: Giampieri, Francesca creator: Battino, Maurizio subject: Alimentación description: The interaction between nutrition and human infectious diseases has always been recognized. With the emergence of molecular tools and post-genomics, high-resolution sequencing technologies, the gut microbiota has been emerging as a key moderator in the complex interplay between nutrients, human body, and infections. Much of the host–microbial and nutrition research is currently based on animals or simplistic in vitro models. Although traditional in vivo and in vitro models have helped to develop mechanistic hypotheses and assess the causality of the host–microbiota interactions, they often fail to faithfully recapitulate the complexity of the human nutrient–microbiome axis in gastrointestinal homeostasis and infections. Over the last decade, remarkable progress in tissue engineering, stem cell biology, microfluidics, sequencing technologies, and computing power has taken place, which has produced a new generation of human-focused, relevant, and predictive tools. These tools, which include patient-derived organoids, organs-on-a-chip, computational analyses, and models, together with multi-omics readouts, represent novel and exciting equipment to advance the research into microbiota, infectious diseases, and nutrition from a human-biology-based perspective. After considering some limitations of the conventional in vivo and in vitro approaches, in this review, we present the main novel available and emerging tools that are suitable for designing human-oriented research. date: 2020-06 type: Artículo type: PeerReviewed identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros Abierto Inglés The interaction between nutrition and human infectious diseases has always been recognized. With the emergence of molecular tools and post-genomics, high-resolution sequencing technologies, the gut microbiota has been emerging as a key moderator in the complex interplay between nutrients, human body, and infections. Much of the host–microbial and nutrition research is currently based on animals or simplistic in vitro models. Although traditional in vivo and in vitro models have helped to develop mechanistic hypotheses and assess the causality of the host–microbiota interactions, they often fail to faithfully recapitulate the complexity of the human nutrient–microbiome axis in gastrointestinal homeostasis and infections. Over the last decade, remarkable progress in tissue engineering, stem cell biology, microfluidics, sequencing technologies, and computing power has taken place, which has produced a new generation of human-focused, relevant, and predictive tools. These tools, which include patient-derived organoids, organs-on-a-chip, computational analyses, and models, together with multi-omics readouts, represent novel and exciting equipment to advance the research into microbiota, infectious diseases, and nutrition from a human-biology-based perspective. After considering some limitations of the conventional in vivo and in vitro approaches, in this review, we present the main novel available and emerging tools that are suitable for designing human-oriented research. metadata Cassotta, Manuela; Forbes-Hernández, Tamara Y.; Calderón Iglesias, Ruben; Ruiz, Roberto; Elexpuru Zabaleta, Maria; Giampieri, Francesca y Battino, Maurizio mail manucassotta@gmail.com, SIN ESPECIFICAR, ruben.calderon@uneatlantico.es, roberto.ruiz@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2020) Links between Nutrition, Infectious Diseases, and Microbiota: Emerging Technologies and Opportunities for Human-Focused Research. Nutrients, 12 (6). p. 1827. ISSN 2072-6643 relation: http://doi.org/10.3390/nu12061827 relation: doi:10.3390/nu12061827 language: en relation: http://repositorio.uneatlantico.es/id/eprint/125/ canonical: http://repositorio.uneatlantico.es/id/eprint/125/ title: Pharmacological, non-pharmacological and stem cell therapies for the management of autism spectrum disorders: A focus on human studies creator: Pistollato, Francesca creator: Forbes-Hernandez, Tamara Y. creator: Calderón Iglesias, Rubén creator: Ruiz Salces, Roberto creator: Elexpuru Zabaleta, Maria creator: Cianciosi, Danila creator: Giampieri, Francesca creator: Battino, Maurizio subject: Biomedicina description: In the last decade, the prevalence of autism spectrum disorders (ASD) has dramatically escalated worldwide. Currently available drugs mainly target some co-occurring symptoms of ASD, but are not effective on the core symptoms, namely impairments in communication and social interaction, and the presence of restricted and repetitive behaviors. On the other hand, transplantation of hematopoietic and mesenchymal stem cells in ASD children has been shown promising to stimulate the recruitment, proliferation, and differentiation of tissue-residing native stem cells, reducing inflammation, and improving some ASD symptoms. Moreover, several comorbidities have also been associated with ASD, such as immune dysregulation, gastrointestinal issues and gut microbiota dysbiosis. Non-pharmacological approaches, such as dietary supplementations with certain vitamins, omega-3 polyunsaturated fatty acids, probiotics, some phytochemicals (e.g., luteolin and sulforaphane), or overall diet interventions (e.g., gluten free and casein free diets) have been considered for the reduction of such comorbidities and the management of ASD. Here, interventional studies describing pharmacological and non-pharmacological treatments in ASD children and adolescents, along with stem cell-based therapies, are reviewed. date: 2020-02 type: Artículo type: PeerReviewed identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Cerrado Inglés In the last decade, the prevalence of autism spectrum disorders (ASD) has dramatically escalated worldwide. Currently available drugs mainly target some co-occurring symptoms of ASD, but are not effective on the core symptoms, namely impairments in communication and social interaction, and the presence of restricted and repetitive behaviors. On the other hand, transplantation of hematopoietic and mesenchymal stem cells in ASD children has been shown promising to stimulate the recruitment, proliferation, and differentiation of tissue-residing native stem cells, reducing inflammation, and improving some ASD symptoms. Moreover, several comorbidities have also been associated with ASD, such as immune dysregulation, gastrointestinal issues and gut microbiota dysbiosis. Non-pharmacological approaches, such as dietary supplementations with certain vitamins, omega-3 polyunsaturated fatty acids, probiotics, some phytochemicals (e.g., luteolin and sulforaphane), or overall diet interventions (e.g., gluten free and casein free diets) have been considered for the reduction of such comorbidities and the management of ASD. Here, interventional studies describing pharmacological and non-pharmacological treatments in ASD children and adolescents, along with stem cell-based therapies, are reviewed. metadata Pistollato, Francesca; Forbes-Hernandez, Tamara Y.; Calderón Iglesias, Rubén; Ruiz Salces, Roberto; Elexpuru Zabaleta, Maria; Cianciosi, Danila; Giampieri, Francesca y Battino, Maurizio mail francesca.pistollato@uneatlantico.es, SIN ESPECIFICAR, ruben.calderon@uneatlantico.es, roberto.ruiz@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2020) Pharmacological, non-pharmacological and stem cell therapies for the management of autism spectrum disorders: A focus on human studies. Pharmacological Research, 152. p. 104579. ISSN 1043-6618 relation: http://doi.org/10.1016/j.phrs.2019.104579 relation: doi:10.1016/j.phrs.2019.104579 language: en relation: http://repositorio.uneatlantico.es/id/eprint/126/ canonical: http://repositorio.uneatlantico.es/id/eprint/126/ title: Effects of caloric restriction on immunosurveillance, microbiota and cancer cell phenotype: Possible implications for cancer treatment creator: Pistollato, Francesca creator: Forbes-Hernández, Tamara Y. creator: Calderón Iglesias, Rubén creator: Ruiz Salces, Roberto creator: Elexpuru Zabaleta, Maria creator: Dominguez Azpíroz, Irma creator: Cianciosi, Danila creator: Quiles, José L. creator: Giampieri, Francesca creator: Battino, Maurizio subject: Biomedicina description: Fasting, caloric restriction and foods or compounds mimicking the biological effects of caloric restriction, known as caloric restriction mimetics, have been associated with a lower risk of age-related diseases, including cardiovascular diseases, cancer and cognitive decline, and a longer lifespan. Reduced calorie intake has been shown to stimulate cancer immunosurveillance, reducing the migration of immunosuppressive regulatory T cells towards the tumor bulk. Autophagy stimulation via reduction of lysine acetylation, increased sensitivity to chemo- and immunotherapy, along with a reduction of insulin-like growth factor 1 and reactive oxygen species have been described as some of the major effects triggered by caloric restriction. Fasting and caloric restriction have also been shown to beneficially influence gut microbiota composition, modify host metabolism, reduce total cholesterol and triglyceride levels, lower diastolic blood pressure and elevate morning cortisol level, with beneficial modulatory effects on cardiopulmonary fitness, body fat and weight, fatigue and weakness, and general quality of life. Moreover, caloric restriction may reduce the carcinogenic and metastatic potential of cancer stem cells, which are generally considered responsible of tumor formation and relapse. Here, we reviewed in vitro and in vivo studies describing the effects of fasting, caloric restriction and some caloric restriction mimetics on immunosurveillance, gut microbiota, metabolism, and cancer stem cell growth, highlighting the molecular and cellular mechanisms underlying these effects. Additionally, studies on caloric restriction interventions in cancer patients or cancer risk subjects are discussed. Considering the promising effects associated with caloric restriction and caloric restriction mimetics, we think that controlled-randomized large clinical trials are warranted to evaluate the inclusion of these non-pharmacological approaches in clinical practice. date: 2021-08 type: Artículo type: PeerReviewed identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica Universidad Internacional do Cuanza > Investigación > Producción Científica Cerrado Inglés Fasting, caloric restriction and foods or compounds mimicking the biological effects of caloric restriction, known as caloric restriction mimetics, have been associated with a lower risk of age-related diseases, including cardiovascular diseases, cancer and cognitive decline, and a longer lifespan. Reduced calorie intake has been shown to stimulate cancer immunosurveillance, reducing the migration of immunosuppressive regulatory T cells towards the tumor bulk. Autophagy stimulation via reduction of lysine acetylation, increased sensitivity to chemo- and immunotherapy, along with a reduction of insulin-like growth factor 1 and reactive oxygen species have been described as some of the major effects triggered by caloric restriction. Fasting and caloric restriction have also been shown to beneficially influence gut microbiota composition, modify host metabolism, reduce total cholesterol and triglyceride levels, lower diastolic blood pressure and elevate morning cortisol level, with beneficial modulatory effects on cardiopulmonary fitness, body fat and weight, fatigue and weakness, and general quality of life. Moreover, caloric restriction may reduce the carcinogenic and metastatic potential of cancer stem cells, which are generally considered responsible of tumor formation and relapse. Here, we reviewed in vitro and in vivo studies describing the effects of fasting, caloric restriction and some caloric restriction mimetics on immunosurveillance, gut microbiota, metabolism, and cancer stem cell growth, highlighting the molecular and cellular mechanisms underlying these effects. Additionally, studies on caloric restriction interventions in cancer patients or cancer risk subjects are discussed. Considering the promising effects associated with caloric restriction and caloric restriction mimetics, we think that controlled-randomized large clinical trials are warranted to evaluate the inclusion of these non-pharmacological approaches in clinical practice. metadata Pistollato, Francesca; Forbes-Hernández, Tamara Y.; Calderón Iglesias, Rubén; Ruiz Salces, Roberto; Elexpuru Zabaleta, Maria; Dominguez Azpíroz, Irma; Cianciosi, Danila; Quiles, José L.; Giampieri, Francesca y Battino, Maurizio mail francesca.pistollato@uneatlantico.es, SIN ESPECIFICAR, ruben.calderon@uneatlantico.es, roberto.ruiz@uneatlantico.es, maria.elexpuru@uneatlantico.es, irma.dominguez@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2021) Effects of caloric restriction on immunosurveillance, microbiota and cancer cell phenotype: Possible implications for cancer treatment. Seminars in Cancer Biology. pp. 45-57. ISSN 1044-579X relation: http://doi.org/10.1016/j.semcancer.2020.11.017 relation: doi:10.1016/j.semcancer.2020.11.017 language: en relation: http://repositorio.uneatlantico.es/id/eprint/117/ canonical: http://repositorio.uneatlantico.es/id/eprint/117/ title: A Diet Rich in Saturated Fat and Cholesterol Aggravates the Effect of Bacterial Lipopolysaccharide on Alveolar Bone Loss in a Rabbit Model of Periodontal Disease creator: Varela-López, Alfonso creator: Bullón, Pedro creator: Ramírez-Tortosa, César L. creator: Navarro-Hortal, María D. creator: Robles-Almazán, María creator: Bullón, Beatriz creator: Cordero, Mario D. creator: Battino, Maurizio creator: Quiles, José L. subject: Biomedicina description: ncreasing evidence connects periodontitis with a variety of systemic diseases, including metabolic syndrome, atherosclerosis, and non-alcoholic fatty liver disease (NAFLD). The proposal of this study was to evaluate the role of diets rich in saturated fat and cholesterol in some aspects of periodontal diseases in a lipopolysaccharide (LPS)-induced model of periodontal disease in rabbits and to assess the influence of a periodontal intervention on hyperlipidemia, atherosclerosis, and NAFLD progression to non-alcoholic steatohepatitis. Male rabbits were maintained on a commercial standard diet or a diet rich in saturated fat (3% lard w/w) and cholesterol (1.3% w/w) (HFD) for 40 days. Half of the rabbits on each diet were treated 2 days per week with intragingival injections of LPS from Porphyromonas gingivalis. Morphometric analyses revealed that LPS induced higher alveolar bone loss (ABL) around the first premolar in animals receiving standard diets, which was exacerbated by the HFD diet. A higher score of acinar inflammation in the liver and higher blood levels of triglycerides and phospholipids were found in HFD-fed rabbits receiving LPS. These results suggest that certain dietary habits can exacerbate some aspects of periodontitis and that bad periodontal health can contribute to dyslipidemia and promote NAFLD progression, but only under certain conditions. date: 2020 type: Artículo type: PeerReviewed format: text language: en rights: cc_by_4 identifier: http://repositorio.uneatlantico.es/id/eprint/117/1/nutrients-12-01405.pdf identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros SIN ESPECIFICAR SIN ESPECIFICAR ncreasing evidence connects periodontitis with a variety of systemic diseases, including metabolic syndrome, atherosclerosis, and non-alcoholic fatty liver disease (NAFLD). The proposal of this study was to evaluate the role of diets rich in saturated fat and cholesterol in some aspects of periodontal diseases in a lipopolysaccharide (LPS)-induced model of periodontal disease in rabbits and to assess the influence of a periodontal intervention on hyperlipidemia, atherosclerosis, and NAFLD progression to non-alcoholic steatohepatitis. Male rabbits were maintained on a commercial standard diet or a diet rich in saturated fat (3% lard w/w) and cholesterol (1.3% w/w) (HFD) for 40 days. Half of the rabbits on each diet were treated 2 days per week with intragingival injections of LPS from Porphyromonas gingivalis. Morphometric analyses revealed that LPS induced higher alveolar bone loss (ABL) around the first premolar in animals receiving standard diets, which was exacerbated by the HFD diet. A higher score of acinar inflammation in the liver and higher blood levels of triglycerides and phospholipids were found in HFD-fed rabbits receiving LPS. These results suggest that certain dietary habits can exacerbate some aspects of periodontitis and that bad periodontal health can contribute to dyslipidemia and promote NAFLD progression, but only under certain conditions. metadata Varela-López, Alfonso; Bullón, Pedro; Ramírez-Tortosa, César L.; Navarro-Hortal, María D.; Robles-Almazán, María; Bullón, Beatriz; Cordero, Mario D.; Battino, Maurizio y Quiles, José L. mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, mario.cordero@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR (2020) A Diet Rich in Saturated Fat and Cholesterol Aggravates the Effect of Bacterial Lipopolysaccharide on Alveolar Bone Loss in a Rabbit Model of Periodontal Disease. Nutrients, 12 (5). p. 1405. ISSN 2072-6643 relation: http://doi.org/10.3390/nu12051405 relation: doi:10.3390/nu12051405 relation: http://repositorio.uneatlantico.es/id/eprint/81/ canonical: http://repositorio.uneatlantico.es/id/eprint/81/ title: Autophagy in Human Health and Disease: Novel Therapeutic Opportunities creator: Giampieri, Francesca creator: Afrin, Sadia creator: Forbes-Hernandez, Tamara Y. creator: Gasparrini, Massimiliano creator: Cianciosi, Danila creator: Reboredo-Rodriguez, Patricia creator: Varela-Lopez, Alfonso creator: Quiles, Jose L. creator: Battino, Maurizio subject: Alimentación description: Significance: In eukaryotes, autophagy represents a highly evolutionary conserved process, through which macromolecules and cytoplasmic material are degraded into lysosomes and recycled for biosynthetic or energetic purposes. Dysfunction of the autophagic process has been associated with the onset and development of many human chronic pathologies, such as cardiovascular, metabolic, and neurodegenerative diseases as well as cancer. Recent Advances: Currently, comprehensive research is being carried out to discover new therapeutic agents that are able to modulate the autophagic process in vivo. Recent evidence has shown that a large number of natural bioactive compounds are involved in the regulation of autophagy by modulating several transcriptional factors and signaling pathways. Critical Issues: Critical issues that deserve particular attention are the inadequate understanding of the complex role of autophagy in disease pathogenesis, the limited availability of therapeutic drugs, and the lack of clinical trials. In this context, the effects that natural bioactive compounds exert on autophagic modulation should be clearly highlighted, since they depend on the type and stage of the pathological conditions of diseases. Future Directions: Research efforts should now focus on understanding the survival-supporting and death-promoting roles of autophagy, how natural compounds interact exactly with the autophagic targets so as to induce or inhibit autophagy and on the evaluation of their pharmacological effects in a more in-depth and mechanistic way. In addition, clinical studies on autophagy-inducing natural products are strongly encouraged, also to highlight some fundamental aspects, such as the dose, the duration, and the possible synergistic action of these compounds with conventional therapy. date: 2019 type: Artículo type: PeerReviewed identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica SIN ESPECIFICAR SIN ESPECIFICAR Significance: In eukaryotes, autophagy represents a highly evolutionary conserved process, through which macromolecules and cytoplasmic material are degraded into lysosomes and recycled for biosynthetic or energetic purposes. Dysfunction of the autophagic process has been associated with the onset and development of many human chronic pathologies, such as cardiovascular, metabolic, and neurodegenerative diseases as well as cancer. Recent Advances: Currently, comprehensive research is being carried out to discover new therapeutic agents that are able to modulate the autophagic process in vivo. Recent evidence has shown that a large number of natural bioactive compounds are involved in the regulation of autophagy by modulating several transcriptional factors and signaling pathways. Critical Issues: Critical issues that deserve particular attention are the inadequate understanding of the complex role of autophagy in disease pathogenesis, the limited availability of therapeutic drugs, and the lack of clinical trials. In this context, the effects that natural bioactive compounds exert on autophagic modulation should be clearly highlighted, since they depend on the type and stage of the pathological conditions of diseases. Future Directions: Research efforts should now focus on understanding the survival-supporting and death-promoting roles of autophagy, how natural compounds interact exactly with the autophagic targets so as to induce or inhibit autophagy and on the evaluation of their pharmacological effects in a more in-depth and mechanistic way. In addition, clinical studies on autophagy-inducing natural products are strongly encouraged, also to highlight some fundamental aspects, such as the dose, the duration, and the possible synergistic action of these compounds with conventional therapy. metadata Giampieri, Francesca; Afrin, Sadia; Forbes-Hernandez, Tamara Y.; Gasparrini, Massimiliano; Cianciosi, Danila; Reboredo-Rodriguez, Patricia; Varela-Lopez, Alfonso; Quiles, Jose L. y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, tamara.forbes@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2019) Autophagy in Human Health and Disease: Novel Therapeutic Opportunities. Antioxidants & Redox Signaling, 30 (4). pp. 577-634. ISSN 1523-0864 relation: http://doi.org/10.1089/ars.2017.7234 relation: doi:10.1089/ars.2017.7234 relation: http://repositorio.uneatlantico.es/id/eprint/82/ canonical: http://repositorio.uneatlantico.es/id/eprint/82/ title: Apis mellifera vs Melipona beecheii Cuban polifloral honeys: A comparison based on their physicochemical parameters, chemical composition and biological properties creator: Alvarez-Suarez, José M. creator: Giampieri, Francesca creator: Brenciani, Andrea creator: Mazzoni, Luca creator: Gasparrini, Massimiliano creator: González-Paramás, Ana M. creator: Santos-Buelga, Celestino creator: Morroni, Gianluca creator: Simoni, Serena creator: Forbes-Hernández, Tamara Y. creator: Afrin, Sadia creator: Giovanetti, Eleonora creator: Battino, Maurizio subject: Alimentación description: Several polifloral honeys from the Apis mellifera and Melipona beecheii bee were analyzed to evaluate their pollen profile, physicochemical parameters, chemical composition and their antioxidant and antimicrobial activity. Flavonoids and other phenolic derivatives, 16 compounds in Apis mellifera honeys and 19 compounds in Melipona beecheii honeys, were identified using the HPLC-DAD-ESI-MS/MS analysis. The physicochemical parameters were within the ranges reported for these honeys, showing some of the distinctive characteristics of M. beecheii honeys compared to A. mellifera honeys, such as their high moisture and acidity. M. beecheii honey showed the highest values of total antioxidant capacity and total phenolic, flavonoid, carotenoids, ascorbic acid, free amino acid and protein contents compared to A. mellifera honey. M. beecheii honey also exhibited a higher antimicrobial activity. Our result shows that M. beecheii honeys is an important source of bioactive compounds with relevant biological properties compared to A. mellifera honey. date: 2018 type: Artículo type: PeerReviewed identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros SIN ESPECIFICAR SIN ESPECIFICAR Several polifloral honeys from the Apis mellifera and Melipona beecheii bee were analyzed to evaluate their pollen profile, physicochemical parameters, chemical composition and their antioxidant and antimicrobial activity. Flavonoids and other phenolic derivatives, 16 compounds in Apis mellifera honeys and 19 compounds in Melipona beecheii honeys, were identified using the HPLC-DAD-ESI-MS/MS analysis. The physicochemical parameters were within the ranges reported for these honeys, showing some of the distinctive characteristics of M. beecheii honeys compared to A. mellifera honeys, such as their high moisture and acidity. M. beecheii honey showed the highest values of total antioxidant capacity and total phenolic, flavonoid, carotenoids, ascorbic acid, free amino acid and protein contents compared to A. mellifera honey. M. beecheii honey also exhibited a higher antimicrobial activity. Our result shows that M. beecheii honeys is an important source of bioactive compounds with relevant biological properties compared to A. mellifera honey. metadata Alvarez-Suarez, José M.; Giampieri, Francesca; Brenciani, Andrea; Mazzoni, Luca; Gasparrini, Massimiliano; González-Paramás, Ana M.; Santos-Buelga, Celestino; Morroni, Gianluca; Simoni, Serena; Forbes-Hernández, Tamara Y.; Afrin, Sadia; Giovanetti, Eleonora y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, tamara.forbes@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2018) Apis mellifera vs Melipona beecheii Cuban polifloral honeys: A comparison based on their physicochemical parameters, chemical composition and biological properties. LWT, 87. pp. 272-279. ISSN 0023-6438 relation: http://doi.org/10.1016/j.lwt.2017.08.079 relation: doi:10.1016/j.lwt.2017.08.079 relation: http://repositorio.uneatlantico.es/id/eprint/83/ canonical: http://repositorio.uneatlantico.es/id/eprint/83/ title: Guava (Psidium guajava L. cv. Red Suprema) Crude Extract Protect Human Dermal Fibroblasts against Cytotoxic Damage Mediated by Oxidative Stress creator: Alvarez-Suarez, José M. creator: Giampieri, Francesca creator: Gasparrini, Massimiliano creator: Mazzoni, Luca creator: Forbes-Hernández, Tamara Y. creator: Afrin, Sadia creator: Battino, Maurizio subject: Alimentación description: We analyzed guava fruits (Psidium guajava L. cv. Red Suprema) from Cuba to determine their chemical composition, total antioxidant capacity, as well as their protective effect against oxidative damage using an in vitro model of human dermal fibroblasts. The guava fruit is a natural source of bioactive compounds, such as polyphenols, vitamin C, folates and beta carotenes with proven health benefits. Human dermal fibroblasts were pre-incubated with different concentrations of guava crude extract and then subjected to oxidative stress using the AAPH stressor. The number of apoptotic and dead cells, as well as the markers of oxidative damage such as lipid and protein oxidation significantly decreased when cells were pre-incubated with guava crude extract and then exposed to the stressor. The activity of antioxidant enzymes also improved when cells were pre-incubated with guava crude extract in comparison to cells subjected to stress without prior pre-incubation with the guava extract. The results obtained in this study highlight the health benefits of guava regarding oxidative stress, proving it to be an important source of bioactive compounds associated with important biological properties. date: 2018 type: Artículo type: PeerReviewed identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros SIN ESPECIFICAR SIN ESPECIFICAR We analyzed guava fruits (Psidium guajava L. cv. Red Suprema) from Cuba to determine their chemical composition, total antioxidant capacity, as well as their protective effect against oxidative damage using an in vitro model of human dermal fibroblasts. The guava fruit is a natural source of bioactive compounds, such as polyphenols, vitamin C, folates and beta carotenes with proven health benefits. Human dermal fibroblasts were pre-incubated with different concentrations of guava crude extract and then subjected to oxidative stress using the AAPH stressor. The number of apoptotic and dead cells, as well as the markers of oxidative damage such as lipid and protein oxidation significantly decreased when cells were pre-incubated with guava crude extract and then exposed to the stressor. The activity of antioxidant enzymes also improved when cells were pre-incubated with guava crude extract in comparison to cells subjected to stress without prior pre-incubation with the guava extract. The results obtained in this study highlight the health benefits of guava regarding oxidative stress, proving it to be an important source of bioactive compounds associated with important biological properties. metadata Alvarez-Suarez, José M.; Giampieri, Francesca; Gasparrini, Massimiliano; Mazzoni, Luca; Forbes-Hernández, Tamara Y.; Afrin, Sadia y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, tamara.forbes@uneatlantico.es, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2018) Guava (Psidium guajava L. cv. Red Suprema) Crude Extract Protect Human Dermal Fibroblasts against Cytotoxic Damage Mediated by Oxidative Stress. Plant Foods for Human Nutrition, 73 (1). pp. 18-24. ISSN 0921-9668 relation: http://doi.org/10.1007/s11130-018-0657-2 relation: doi:10.1007/s11130-018-0657-2 relation: http://repositorio.uneatlantico.es/id/eprint/84/ canonical: http://repositorio.uneatlantico.es/id/eprint/84/ title: Nrf2 as regulator of innate immunity: A molecular Swiss army knife! creator: Battino, Maurizio creator: Giampieri, Francesca creator: Pistollato, Francesca creator: Sureda, Antoni creator: de Oliveira, Marcos Roberto creator: Pittalà, Valeria creator: Fallarino, Francesca creator: Nabavi, Seyed Fazel creator: Atanasov, Atanas Georgiev creator: Nabavi, Seyed Mohammad subject: Alimentación description: Organisms are constantly exposed to a broad range of pathological and stress-inducing agents, allergens and environmental chemicals that can induce infections, toxicity or other undesirable reactions. Our immune system has evolved over time in order to efficiently respond to these exogenous insults and maintain homeostasis. In particular, the innate immune system acts as primary barrier to prevent the entrance of invasive agents or allergens. This system is comprised of a diversity of cell types that are rapidly activated by recognition of common structures present in many potential pathogens known as pathogen-associated molecular patterns (PAMPs). The nuclear factor erythroid 2 related factor 2 (Nrf2) is a relevant basic leucine zipper (bZIP) transcription factor that is essential in the regulation of cell cycle homeostasis, cytoprotection, and innate immunity when cells are under stressful conditions. Although the role of Nrf2 in activating the expression of protective genes – such as antioxidant or anti-inflammatory – is known, its role in innate immunity and immune-related gene expression remains not yet clear. The present review summarizes current knowledge on Nrf2 signaling pathway structure and activity under both physiological state and upon oxidative stress. In addition, the relation between Nrf2 signaling pathway and the innate immune system is discussed, highlighting the potential therapeutic effects of diverse natural and synthetic compounds as Nrf2 regulators. date: 2018 type: Artículo type: PeerReviewed identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros SIN ESPECIFICAR SIN ESPECIFICAR Organisms are constantly exposed to a broad range of pathological and stress-inducing agents, allergens and environmental chemicals that can induce infections, toxicity or other undesirable reactions. Our immune system has evolved over time in order to efficiently respond to these exogenous insults and maintain homeostasis. In particular, the innate immune system acts as primary barrier to prevent the entrance of invasive agents or allergens. This system is comprised of a diversity of cell types that are rapidly activated by recognition of common structures present in many potential pathogens known as pathogen-associated molecular patterns (PAMPs). The nuclear factor erythroid 2 related factor 2 (Nrf2) is a relevant basic leucine zipper (bZIP) transcription factor that is essential in the regulation of cell cycle homeostasis, cytoprotection, and innate immunity when cells are under stressful conditions. Although the role of Nrf2 in activating the expression of protective genes – such as antioxidant or anti-inflammatory – is known, its role in innate immunity and immune-related gene expression remains not yet clear. The present review summarizes current knowledge on Nrf2 signaling pathway structure and activity under both physiological state and upon oxidative stress. In addition, the relation between Nrf2 signaling pathway and the innate immune system is discussed, highlighting the potential therapeutic effects of diverse natural and synthetic compounds as Nrf2 regulators. metadata Battino, Maurizio; Giampieri, Francesca; Pistollato, Francesca; Sureda, Antoni; de Oliveira, Marcos Roberto; Pittalà, Valeria; Fallarino, Francesca; Nabavi, Seyed Fazel; Atanasov, Atanas Georgiev y Nabavi, Seyed Mohammad mail SIN ESPECIFICAR, SIN ESPECIFICAR, francesca.pistollato@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2018) Nrf2 as regulator of innate immunity: A molecular Swiss army knife! Biotechnology Advances, 36 (2). pp. 358-370. ISSN 0734-9750 relation: http://doi.org/10.1016/j.biotechadv.2017.12.012 relation: doi:10.1016/j.biotechadv.2017.12.012 relation: http://repositorio.uneatlantico.es/id/eprint/85/ canonical: http://repositorio.uneatlantico.es/id/eprint/85/ title: Intracellular signaling pathways of inflammation modulated by dietary flavonoids: The most recent evidence creator: Chen, Lei creator: Teng, Hui creator: Jia, Zhen creator: Battino, Maurizio creator: Miron, Anca creator: Yu, Zhiling creator: Cao, Hui creator: Xiao, Jianbo subject: Alimentación description: Dietary flavonoids, which occur in many plant foods, are considered as the most active constituents among the plant-derived ones in vitro and in vivo. To date, many studies have addressed the anti-inflammatory activity of flavonoids. However, their considerable structural diversity and in vivo bioavailability make them able to modulate different signaling pathways. The present review attempted to summarize and highlight a broad range of inflammation-associated signaling pathways modulated by flavonoids. Finally, based on the current scientist's literature, structure-activity relationships were concluded. Dietary flavonoids have the ability to attenuate inflammation by targeting different intracellular signaling pathways triggered by NF-κB, AP-1, PPAR, Nrf2, and MAPKs. Identification of the main structural features required for the modulation of these inflammation-related pathways (hydroxylation pattern, C2=C3 double bond) have an important role to play in the development of new anti-inflammatory drugs. date: 2018 type: Artículo type: PeerReviewed identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros SIN ESPECIFICAR SIN ESPECIFICAR Dietary flavonoids, which occur in many plant foods, are considered as the most active constituents among the plant-derived ones in vitro and in vivo. To date, many studies have addressed the anti-inflammatory activity of flavonoids. However, their considerable structural diversity and in vivo bioavailability make them able to modulate different signaling pathways. The present review attempted to summarize and highlight a broad range of inflammation-associated signaling pathways modulated by flavonoids. Finally, based on the current scientist's literature, structure-activity relationships were concluded. Dietary flavonoids have the ability to attenuate inflammation by targeting different intracellular signaling pathways triggered by NF-κB, AP-1, PPAR, Nrf2, and MAPKs. Identification of the main structural features required for the modulation of these inflammation-related pathways (hydroxylation pattern, C2=C3 double bond) have an important role to play in the development of new anti-inflammatory drugs. metadata Chen, Lei; Teng, Hui; Jia, Zhen; Battino, Maurizio; Miron, Anca; Yu, Zhiling; Cao, Hui y Xiao, Jianbo mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2018) Intracellular signaling pathways of inflammation modulated by dietary flavonoids: The most recent evidence. Critical Reviews in Food Science and Nutrition, 58 (17). pp. 2908-2924. ISSN 1040-8398 relation: http://doi.org/10.1080/10408398.2017.1345853 relation: doi:10.1080/10408398.2017.1345853 relation: http://repositorio.uneatlantico.es/id/eprint/86/ canonical: http://repositorio.uneatlantico.es/id/eprint/86/ title: Phenolic Compounds in Honey and Their Associated Health Benefits: A Review creator: Cianciosi, Danila creator: Forbes-Hernández, Tamara Y. creator: Afrin, Sadia creator: Gasparrini, Massimiliano creator: Reboredo-Rodriguez, Patricia creator: Manna, Piera creator: Zhang, Jiaojiao creator: Bravo Lamas, Leire creator: Martínez Flórez, Susana creator: Agudo Toyos, Pablo creator: Quiles, José creator: Giampieri, Francesca creator: Battino, Maurizio subject: Alimentación description: Honey is a natural substance appreciated for its therapeutic abilities since ancient times. Its content in flavonoids and phenolic acids plays a key role on human health, thanks to the high antioxidant and anti-inflammatory properties that they exert. Honey possesses antimicrobial capacity and anticancer activity against different types of tumors, acting on different molecular pathways that are involved on cellular proliferation. In addition, an antidiabetic activity has also been highlighted, with the reduction of glucose, fructosamine, and glycosylated hemoglobin serum concentration. Honey exerts also a protective effect in the cardiovascular system, where it mainly prevents the oxidation of low-density lipoproteins, in the nervous system, in the respiratory system against asthma and bacterial infections, and in the gastrointestinal system. A beneficial effect of honey can also be demonstrated in athletes. The purpose of this review is to summarize and update the current information regarding the role of honey in health and diseases. date: 2018 type: Artículo type: PeerReviewed format: text language: en rights: cc_by_4 identifier: http://repositorio.uneatlantico.es/id/eprint/86/1/molecules-23-02322-v2.pdf identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros SIN ESPECIFICAR SIN ESPECIFICAR Honey is a natural substance appreciated for its therapeutic abilities since ancient times. Its content in flavonoids and phenolic acids plays a key role on human health, thanks to the high antioxidant and anti-inflammatory properties that they exert. Honey possesses antimicrobial capacity and anticancer activity against different types of tumors, acting on different molecular pathways that are involved on cellular proliferation. In addition, an antidiabetic activity has also been highlighted, with the reduction of glucose, fructosamine, and glycosylated hemoglobin serum concentration. Honey exerts also a protective effect in the cardiovascular system, where it mainly prevents the oxidation of low-density lipoproteins, in the nervous system, in the respiratory system against asthma and bacterial infections, and in the gastrointestinal system. A beneficial effect of honey can also be demonstrated in athletes. The purpose of this review is to summarize and update the current information regarding the role of honey in health and diseases. metadata Cianciosi, Danila; Forbes-Hernández, Tamara Y.; Afrin, Sadia; Gasparrini, Massimiliano; Reboredo-Rodriguez, Patricia; Manna, Piera; Zhang, Jiaojiao; Bravo Lamas, Leire; Martínez Flórez, Susana; Agudo Toyos, Pablo; Quiles, José; Giampieri, Francesca y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, leire.bravo@uneatlantico.es, susana.martínez@uneatlantico.es, pablo.agudo@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2018) Phenolic Compounds in Honey and Their Associated Health Benefits: A Review. Molecules, 23 (9). p. 2322. ISSN 1420-3049 relation: http://doi.org/10.3390/molecules23092322 relation: doi:10.3390/molecules23092322 relation: http://repositorio.uneatlantico.es/id/eprint/129/ canonical: http://repositorio.uneatlantico.es/id/eprint/129/ title: Strawberry extracts efficiently counteract inflammatory stress induced by the endotoxin lipopolysaccharide in Human Dermal Fibroblast creator: Gasparrini, Massimiliano creator: Giampieri, Francesca creator: Forbes-Hernandez, Tamara Y. creator: Afrin, Sadia creator: Cianciosi, Danila creator: Reboredo-Rodriguez, Patricia creator: Varela-Lopez, Alfonso creator: Zhang, JiaoJiao creator: Quiles, Josè L. creator: Mezzetti, Bruno creator: Bompadre, Stefano creator: Battino, Maurizio subject: Biomedicina description: A protracted pro-inflammatory state is the common denominator in the development, progression and complication of the common chronic diseases. Dietary antioxidants represent an efficient tool to counteract this inflammatory state. The aim of the present work was to evaluate the effects of strawberry extracts on inflammation evoked by E. Coli lipopolysaccharide in Human Dermal Fibroblast, by measuring reactive oxygen species production, apoptosis rate, antioxidant enzymes activity, mitochondria functionality and also investigating the molecular pathway involved in inflammatory and antioxidant response. The results demonstrated that strawberry pre-treatment reduced intracellular reactive oxygen species levels, apoptotic rate, improved antioxidant defences and mitochondria functionality in lipopolysaccharide -treated cells. Strawberry exerted these protective activities through the inhibition of the NF-kB signalling pathway and the stimulation of the Nrf2 pathway, with a mechanism AMPK-dependent. These results confirm the health benefits of strawberry in the prevention of inflammation and oxidative stress condition in lipopolysaccharide-treated cells. date: 2018 type: Artículo type: PeerReviewed identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica Cerrado Inglés A protracted pro-inflammatory state is the common denominator in the development, progression and complication of the common chronic diseases. Dietary antioxidants represent an efficient tool to counteract this inflammatory state. The aim of the present work was to evaluate the effects of strawberry extracts on inflammation evoked by E. Coli lipopolysaccharide in Human Dermal Fibroblast, by measuring reactive oxygen species production, apoptosis rate, antioxidant enzymes activity, mitochondria functionality and also investigating the molecular pathway involved in inflammatory and antioxidant response. The results demonstrated that strawberry pre-treatment reduced intracellular reactive oxygen species levels, apoptotic rate, improved antioxidant defences and mitochondria functionality in lipopolysaccharide -treated cells. Strawberry exerted these protective activities through the inhibition of the NF-kB signalling pathway and the stimulation of the Nrf2 pathway, with a mechanism AMPK-dependent. These results confirm the health benefits of strawberry in the prevention of inflammation and oxidative stress condition in lipopolysaccharide-treated cells. metadata Gasparrini, Massimiliano; Giampieri, Francesca; Forbes-Hernandez, Tamara Y.; Afrin, Sadia; Cianciosi, Danila; Reboredo-Rodriguez, Patricia; Varela-Lopez, Alfonso; Zhang, JiaoJiao; Quiles, Josè L.; Mezzetti, Bruno; Bompadre, Stefano y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, tamara.forbes@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2018) Strawberry extracts efficiently counteract inflammatory stress induced by the endotoxin lipopolysaccharide in Human Dermal Fibroblast. Food and Chemical Toxicology, 114. pp. 128-140. ISSN 0278-6915 relation: http://doi.org/10.1016/j.fct.2018.02.038 relation: doi:10.1016/j.fct.2018.02.038 language: en relation: http://repositorio.uneatlantico.es/id/eprint/130/ canonical: http://repositorio.uneatlantico.es/id/eprint/130/ title: Phytochemical Composition and Cytotoxic Effects on Liver Hepatocellular Carcinoma Cells of Different Berries Following a Simulated In Vitro Gastrointestinal Digestion creator: Giampieri, Francesca creator: Afrin, Sadia creator: Stewart, Derek creator: McDougall, Gordon creator: Brennan, Rex creator: Blyth, Lesley creator: Gasparrini, Massimiliano creator: Mazzoni, Luca creator: Capocasa, Franco creator: Alvarez-Suarez, José M. creator: Bompadre, Stefano creator: Nogueira Brás de Oliveira, Pedro creator: N. Santos, Claudia creator: Masias Vergara, Manuel creator: Agudo Toyos, Pablo creator: Crespo-Álvarez, Jorge creator: Mezzetti, Bruno creator: Forbes-Hernandez, Tamara Y. creator: Battino, Maurizio subject: Biomedicina description: Berry fruits are rich in nutrients and polyphenols, providing potential health benefits. Understanding the factors that affect their bioavailability is becoming of utmost importance for evaluating their biological significance and efficacy as functional food. In this study, the phytochemical composition and the total antioxidant capacity of different varieties of five berries (blackberry, blackcurrant, blueberry, raspberry, and strawberry) were evaluated after an in vitro gastrointestinal digestion process. The cultivar of each berry that showed the higher content of total phenols and flavonoids was selected to study its cytotoxic effect on human hepatoma cells. Digestion resulted in a high reduction (p ˂ 0.05) of total phenolic, flavonoid and anthocyanin contents and total antioxidant capacity, in the “IN” samples compared to the “OUT” extracts, which represent the “serum-available” and the “colon-available” fractions, respectively. Incubation of the digested fraction for 24 h didn’t exert any effect on cellular viability, while a dose- and time-dependent cytotoxicity was observed after 48 h and 72 h of incubation for all the berries analyzed. Our results suggest that the approach proposed in this work may represent a rapid tool for evaluating and identifying new berries with increased phytochemical bioavailability, highlighting their antiproliferative agents after an in vitro digestion. date: 2018 type: Artículo type: PeerReviewed format: text language: en rights: cc_by_4 identifier: http://repositorio.uneatlantico.es/id/eprint/130/1/molecules-23-01918.pdf identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros SIN ESPECIFICAR SIN ESPECIFICAR Berry fruits are rich in nutrients and polyphenols, providing potential health benefits. Understanding the factors that affect their bioavailability is becoming of utmost importance for evaluating their biological significance and efficacy as functional food. In this study, the phytochemical composition and the total antioxidant capacity of different varieties of five berries (blackberry, blackcurrant, blueberry, raspberry, and strawberry) were evaluated after an in vitro gastrointestinal digestion process. The cultivar of each berry that showed the higher content of total phenols and flavonoids was selected to study its cytotoxic effect on human hepatoma cells. Digestion resulted in a high reduction (p ˂ 0.05) of total phenolic, flavonoid and anthocyanin contents and total antioxidant capacity, in the “IN” samples compared to the “OUT” extracts, which represent the “serum-available” and the “colon-available” fractions, respectively. Incubation of the digested fraction for 24 h didn’t exert any effect on cellular viability, while a dose- and time-dependent cytotoxicity was observed after 48 h and 72 h of incubation for all the berries analyzed. Our results suggest that the approach proposed in this work may represent a rapid tool for evaluating and identifying new berries with increased phytochemical bioavailability, highlighting their antiproliferative agents after an in vitro digestion. metadata Giampieri, Francesca; Afrin, Sadia; Stewart, Derek; McDougall, Gordon; Brennan, Rex; Blyth, Lesley; Gasparrini, Massimiliano; Mazzoni, Luca; Capocasa, Franco; Alvarez-Suarez, José M.; Bompadre, Stefano; Nogueira Brás de Oliveira, Pedro; N. Santos, Claudia; Masias Vergara, Manuel; Agudo Toyos, Pablo; Crespo-Álvarez, Jorge; Mezzetti, Bruno; Forbes-Hernandez, Tamara Y. y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, manuel.masias@uneatlantico.es, pablo.agudo@uneatlantico.es, jorge.crespo@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2018) Phytochemical Composition and Cytotoxic Effects on Liver Hepatocellular Carcinoma Cells of Different Berries Following a Simulated In Vitro Gastrointestinal Digestion. Molecules, 23 (8). p. 1918. ISSN 1420-3049 relation: http://doi.org/10.3390/molecules23081918 relation: doi:10.3390/molecules23081918 relation: http://repositorio.uneatlantico.es/id/eprint/87/ canonical: http://repositorio.uneatlantico.es/id/eprint/87/ title: Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells creator: Giampieri, Francesca creator: Gasparrini, Massimiliano creator: Forbes-Hernandez, Tamara Y. creator: Mazzoni, Luca creator: Capocasa, Franco creator: Sabbadini, Silvia creator: Alvarez-Suarez, José M. creator: Afrin, Sadia creator: Rosati, Carlo creator: Pandolfini, Tiziana creator: Molesini, Barbara creator: Sánchez-Sevilla, José Federico creator: Amaya, Iraida creator: Mezzetti, Bruno creator: Battino, Maurizio subject: Alimentación description: Food fortification through the increase and/or modulation of bioactive compounds has become a major goal for preventing several diseases, including cancer. Here, strawberry lines of cv. Calypso transformed with a construct containing an anthocyanidin synthase (ANS) gene were produced to study the effects on anthocyanin biosynthesis, metabolism, and transcriptome. Three strawberry ANS transgenic lines (ANS L5, ANS L15, and ANS L18) were analyzed for phytochemical composition and total antioxidant capacity (TAC), and their fruit extracts were assessed for cytotoxic effects on hepatocellular carcinoma. ANS L18 fruits had the highest levels of total phenolics and flavonoids, while those of ANS L15 had the highest anthocyanin concentration; TAC positively correlated with total polyphenol content. Fruit transcriptome was also specifically affected in the polyphenol biosynthesis and in other related metabolic pathways. Fruit extracts of all lines exerted cytotoxic effects in a dose/time-dependent manner, increasing cellular apoptosis and free radical levels and impairing mitochondrial functionality. date: 2018 type: Artículo type: PeerReviewed identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros SIN ESPECIFICAR SIN ESPECIFICAR Food fortification through the increase and/or modulation of bioactive compounds has become a major goal for preventing several diseases, including cancer. Here, strawberry lines of cv. Calypso transformed with a construct containing an anthocyanidin synthase (ANS) gene were produced to study the effects on anthocyanin biosynthesis, metabolism, and transcriptome. Three strawberry ANS transgenic lines (ANS L5, ANS L15, and ANS L18) were analyzed for phytochemical composition and total antioxidant capacity (TAC), and their fruit extracts were assessed for cytotoxic effects on hepatocellular carcinoma. ANS L18 fruits had the highest levels of total phenolics and flavonoids, while those of ANS L15 had the highest anthocyanin concentration; TAC positively correlated with total polyphenol content. Fruit transcriptome was also specifically affected in the polyphenol biosynthesis and in other related metabolic pathways. Fruit extracts of all lines exerted cytotoxic effects in a dose/time-dependent manner, increasing cellular apoptosis and free radical levels and impairing mitochondrial functionality. metadata Giampieri, Francesca; Gasparrini, Massimiliano; Forbes-Hernandez, Tamara Y.; Mazzoni, Luca; Capocasa, Franco; Sabbadini, Silvia; Alvarez-Suarez, José M.; Afrin, Sadia; Rosati, Carlo; Pandolfini, Tiziana; Molesini, Barbara; Sánchez-Sevilla, José Federico; Amaya, Iraida; Mezzetti, Bruno y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2018) Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells. Journal of Agricultural and Food Chemistry, 66 (3). pp. 581-592. ISSN 0021-8561 relation: http://doi.org/10.1021/acs.jafc.7b04177 relation: doi:10.1021/acs.jafc.7b04177 relation: http://repositorio.uneatlantico.es/id/eprint/131/ canonical: http://repositorio.uneatlantico.es/id/eprint/131/ title: Are by-products from beeswax recycling process a new promising source of bioactive compounds with biomedical properties? creator: Giampieri, Francesca creator: Quiles, José L. creator: Orantes-Bermejo, Francisco J. creator: Gasparrini, Massimiliano creator: Forbes-Hernandez, Tamara Y. creator: Sánchez-González, Cristina creator: Llopis, Juan creator: Rivas-García, Lorenzo creator: Afrin, Sadia creator: Varela-López, Alfonso creator: Cianciosi, Danila creator: Reboredo-Rodriguez, Patricia creator: Fernández-Piñar, Cristina Torres creator: Caderón Iglesia, Rubén creator: Ruiz Salces, Roberto creator: Aparicio Obregón, Silvia creator: Crespo-Álvarez, Jorge creator: Dzul Lopez, Luis creator: Xiao, Jianbo creator: Battino, Maurizio subject: Biomedicina description: During the process of beeswax recycling, many industrial derivatives are obtained. These matrices may have an interesting healthy and commercial potential but to date they have not been properly studied. The aim of the present work was to evaluate the proximal and phytochemical composition, the antioxidant capacity and cytotoxic effects of two by-products from beeswax recycling process named MUD 1 and MUD 2 on liver hepatocellular carcinoma. Our results showed that MUD 1 presented the highest (P < .05) fiber, protein, carbohydrate, polyphenol and flavonoid concentration, as well as the highest (P < .05) total antioxidant capacity than the MUD 2 samples. MUD1 exerted also anticancer activity on HepG2 cells, by reducing cellular viability, increasing intracellular ROS levels and affecting mitochondrial functionality in a dose-dependent manner. We showed for the first time that by-products from beeswax recycling process can represent a rich source of phytochemicals with high total antioxidant capacity and anticancer activity; however, further researches are necessary to evaluate their potentiality for human health by in vivo studies. date: 2018 type: Artículo type: PeerReviewed identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica Cerrado Inglés During the process of beeswax recycling, many industrial derivatives are obtained. These matrices may have an interesting healthy and commercial potential but to date they have not been properly studied. The aim of the present work was to evaluate the proximal and phytochemical composition, the antioxidant capacity and cytotoxic effects of two by-products from beeswax recycling process named MUD 1 and MUD 2 on liver hepatocellular carcinoma. Our results showed that MUD 1 presented the highest (P < .05) fiber, protein, carbohydrate, polyphenol and flavonoid concentration, as well as the highest (P < .05) total antioxidant capacity than the MUD 2 samples. MUD1 exerted also anticancer activity on HepG2 cells, by reducing cellular viability, increasing intracellular ROS levels and affecting mitochondrial functionality in a dose-dependent manner. We showed for the first time that by-products from beeswax recycling process can represent a rich source of phytochemicals with high total antioxidant capacity and anticancer activity; however, further researches are necessary to evaluate their potentiality for human health by in vivo studies. metadata Giampieri, Francesca; Quiles, José L.; Orantes-Bermejo, Francisco J.; Gasparrini, Massimiliano; Forbes-Hernandez, Tamara Y.; Sánchez-González, Cristina; Llopis, Juan; Rivas-García, Lorenzo; Afrin, Sadia; Varela-López, Alfonso; Cianciosi, Danila; Reboredo-Rodriguez, Patricia; Fernández-Piñar, Cristina Torres; Caderón Iglesia, Rubén; Ruiz Salces, Roberto; Aparicio Obregón, Silvia; Crespo-Álvarez, Jorge; Dzul Lopez, Luis; Xiao, Jianbo y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, ruben.calderon@uneatlantico.es, roberto.ruiz@uneatlantico.es, silvia.aparicio@uneatlantico.es, jorge.crespo@uneatlantico.es, luis.dzul@unini.edu.mx, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2018) Are by-products from beeswax recycling process a new promising source of bioactive compounds with biomedical properties? Food and Chemical Toxicology, 112. pp. 126-133. ISSN 0278-6915 relation: http://doi.org/10.1016/j.fct.2017.12.041 relation: doi:10.1016/j.fct.2017.12.041 language: en relation: http://repositorio.uneatlantico.es/id/eprint/132/ canonical: http://repositorio.uneatlantico.es/id/eprint/132/ title: Strawberry-Tree Honey Induces Growth Inhibition of Human Colon Cancer Cells and Increases ROS Generation: A Comparison with Manuka Honey creator: Afrin, Sadia creator: Forbes-Hernandez, Tamara Y. creator: Gasparrini, Massimiliano creator: Bompadre, Stefano creator: Quiles, José creator: Sanna, Gavino creator: Spano, Nadia creator: Giampieri, Francesca creator: Battino, Maurizio subject: Biomedicina description: Honey is a natural product known to modulate several biological activities including cancer. The aim of the present study was to examine the phytochemical content and the antioxidant activity of Strawberry tree (Arbutus unedo) honey (STH) and its cytotoxic properties against human colon adenocarcinoma (HCT-116) and metastatic (LoVo) cell lines in comparison with Manuka (Leptospermum scoparium) honey (MH). Several unifloral STH and MH were analyzed for their phenolic, flavonoid, amino acid and protein contents, as well as their radical scavenging activities. STH from the Berchidda area showed the highest amount of phenolic, flavonoid, amino acid and protein content, and antioxidant capacity compared to MH. Both STH and MH induced cytotoxicity and cell death in a dose- and time-dependent manner in HCT-116 and LoVo cells, with less toxicity on non-cancer cells. Compared to MH, STH showed more effect at lower concentrations on HCT-116 and LoVo cells. In addition, both honeys increased intracellular reactive oxygen species (ROS) generation. In HCT-116 cells, STH and MH induced similar ROS production but in LoVo cells STH induced a higher percentage of ROS compared to MH. Our results indicate that STH and MH can induce cell growth inhibition and ROS generation in colon adenocarcinoma and metastatic cells, which could be due to the presence of phytochemicals with antioxidant properties. These preliminary results are interesting and suggest a potential chemopreventive action which could be useful for further studies in order to develop chemopreventive agents for colon cancer. date: 2017 type: Artículo type: PeerReviewed format: text language: en rights: cc_by_4 identifier: http://repositorio.uneatlantico.es/id/eprint/132/1/ijms-18-00613.pdf identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros SIN ESPECIFICAR SIN ESPECIFICAR Honey is a natural product known to modulate several biological activities including cancer. The aim of the present study was to examine the phytochemical content and the antioxidant activity of Strawberry tree (Arbutus unedo) honey (STH) and its cytotoxic properties against human colon adenocarcinoma (HCT-116) and metastatic (LoVo) cell lines in comparison with Manuka (Leptospermum scoparium) honey (MH). Several unifloral STH and MH were analyzed for their phenolic, flavonoid, amino acid and protein contents, as well as their radical scavenging activities. STH from the Berchidda area showed the highest amount of phenolic, flavonoid, amino acid and protein content, and antioxidant capacity compared to MH. Both STH and MH induced cytotoxicity and cell death in a dose- and time-dependent manner in HCT-116 and LoVo cells, with less toxicity on non-cancer cells. Compared to MH, STH showed more effect at lower concentrations on HCT-116 and LoVo cells. In addition, both honeys increased intracellular reactive oxygen species (ROS) generation. In HCT-116 cells, STH and MH induced similar ROS production but in LoVo cells STH induced a higher percentage of ROS compared to MH. Our results indicate that STH and MH can induce cell growth inhibition and ROS generation in colon adenocarcinoma and metastatic cells, which could be due to the presence of phytochemicals with antioxidant properties. These preliminary results are interesting and suggest a potential chemopreventive action which could be useful for further studies in order to develop chemopreventive agents for colon cancer. metadata Afrin, Sadia; Forbes-Hernandez, Tamara Y.; Gasparrini, Massimiliano; Bompadre, Stefano; Quiles, José; Sanna, Gavino; Spano, Nadia; Giampieri, Francesca y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2017) Strawberry-Tree Honey Induces Growth Inhibition of Human Colon Cancer Cells and Increases ROS Generation: A Comparison with Manuka Honey. International Journal of Molecular Sciences, 18 (3). p. 613. ISSN 1422-0067 relation: http://doi.org/10.3390/ijms18030613 relation: doi:10.3390/ijms18030613 relation: http://repositorio.uneatlantico.es/id/eprint/88/ canonical: http://repositorio.uneatlantico.es/id/eprint/88/ title: Anti-inflammatory effect of Capuli cherry against LPS-induced cytotoxic damage in RAW 264.7 macrophages creator: Alvarez-Suarez, José M. creator: Carrillo-Perdomo, Estefanía creator: Aller, Angel creator: Giampieri, Francesca creator: Gasparrini, Massimiliano creator: González-Pérez, Lien creator: Beltrán-Ayala, Pablo creator: Battino, Maurizio subject: Alimentación description: Capuli cherry (Prunus serotina Ehr. subsp. capuli (Cav.) McVaugh) fruits from the inter-Andean region of Ecuador were analysed to determine their bioactive compounds content, total antioxidant capacity, radical scavenging activity and their anti-inflammatory and protective effects against the cytotoxic damage mediated by lipopolysaccharide (LPS) in RAW 264.7 macrophages. Capuli fruits proved to be a natural source of bioactive compounds such as anthocyanins, vitamin C and β-carotene as well as to present an important total antioxidant capacity and radical scavenging activities. RAW 264.7 macrophages were incubated with different concentration of Capuli crude extract and subsequently activated by LPS to determine the markers related to oxidative damage and the proinflammatory cytokine production. The markers of oxidative damage, nitrite levels, the interleukin 1β messenger RNA levels and the tumor necrosis factor α mRNA levels and secretion were significantly decreased after the pre-incubated with Capuli extract and subsequently stimulated with LPS. In summary, Capuli extract attenuated the LPS-induced damage in RAW 264.7 macrophages due to its antioxidant and anti-inflammatory properties, showing that Capuli fruits may represent a relevant source of bioactive compounds with promising benefits for human health. date: 2017 type: Artículo type: PeerReviewed identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros SIN ESPECIFICAR SIN ESPECIFICAR Capuli cherry (Prunus serotina Ehr. subsp. capuli (Cav.) McVaugh) fruits from the inter-Andean region of Ecuador were analysed to determine their bioactive compounds content, total antioxidant capacity, radical scavenging activity and their anti-inflammatory and protective effects against the cytotoxic damage mediated by lipopolysaccharide (LPS) in RAW 264.7 macrophages. Capuli fruits proved to be a natural source of bioactive compounds such as anthocyanins, vitamin C and β-carotene as well as to present an important total antioxidant capacity and radical scavenging activities. RAW 264.7 macrophages were incubated with different concentration of Capuli crude extract and subsequently activated by LPS to determine the markers related to oxidative damage and the proinflammatory cytokine production. The markers of oxidative damage, nitrite levels, the interleukin 1β messenger RNA levels and the tumor necrosis factor α mRNA levels and secretion were significantly decreased after the pre-incubated with Capuli extract and subsequently stimulated with LPS. In summary, Capuli extract attenuated the LPS-induced damage in RAW 264.7 macrophages due to its antioxidant and anti-inflammatory properties, showing that Capuli fruits may represent a relevant source of bioactive compounds with promising benefits for human health. metadata Alvarez-Suarez, José M.; Carrillo-Perdomo, Estefanía; Aller, Angel; Giampieri, Francesca; Gasparrini, Massimiliano; González-Pérez, Lien; Beltrán-Ayala, Pablo y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2017) Anti-inflammatory effect of Capuli cherry against LPS-induced cytotoxic damage in RAW 264.7 macrophages. Food and Chemical Toxicology, 102. pp. 46-52. ISSN 0278-6915 relation: http://doi.org/10.1016/j.fct.2017.01.024 relation: doi:10.1016/j.fct.2017.01.024 relation: http://repositorio.uneatlantico.es/id/eprint/133/ canonical: http://repositorio.uneatlantico.es/id/eprint/133/ title: The protective effect of acerola (Malpighia emarginata) against oxidative damage in human dermal fibroblasts through the improvement of antioxidant enzyme activity and mitochondrial functionality creator: Alvarez-Suarez, José M. creator: Giampieri, Francesca creator: Gasparrini, Massimiliano creator: Mazzoni, Luca creator: Santos-Buelga, Celestino creator: González-Paramás, Ana M. creator: Forbes-Hernández, Tamara Y. creator: Afrin, Sadia creator: Páez-Watson, Timothy creator: Quiles, José L. creator: Battino, Maurizio subject: Biomedicina description: Acerola fruits (Malpighia emarginata DC.) from the central region of Cuba were analyzed to determine their chemical composition and protective capacity against oxidative damage using an in vitro human dermal fibroblast (HDFa) model. The chemical composition analyses showed a high content of vitamin C, total polyphenols, β-carotene and folates in the acerola fruit. From the HPLC-DAD/ESI-MSn analyses, two anthocyanins (cyanidin 3-O-rhamnoside and pelargonidin 3-O-rhamnoside), three hydroxycinnamoyl derivatives (caffeoyl hexoside, dihydrocaffeoylquinic acid and coumaroyl hexoside) and fifteen flavonols (mostly glycosylated forms of quercetin and kaempferol) were detected. HDFa were pre-incubated with an acerola crude extract (ACExt) and subsequently subjected to oxidative stress induced by AAPH. Apoptosis, intracellular ROS and the biomarkers of lipid and protein oxidation significantly increased after inducing stress, while the activities of the antioxidant enzyme catalase and superoxide dismutase and mitochondrial functionality were markedly affected. However, ACExt was able to protect against oxidative damage through decreasing apoptosis, intracellular ROS levels and lipid and protein damage, besides improving antioxidant enzyme activities and mitochondrial functionality. The obtained results support acerola fruits as relevant sources of functional compounds with promising effects on human health. date: 2017 type: Artículo type: PeerReviewed identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Cerrado Inglés Acerola fruits (Malpighia emarginata DC.) from the central region of Cuba were analyzed to determine their chemical composition and protective capacity against oxidative damage using an in vitro human dermal fibroblast (HDFa) model. The chemical composition analyses showed a high content of vitamin C, total polyphenols, β-carotene and folates in the acerola fruit. From the HPLC-DAD/ESI-MSn analyses, two anthocyanins (cyanidin 3-O-rhamnoside and pelargonidin 3-O-rhamnoside), three hydroxycinnamoyl derivatives (caffeoyl hexoside, dihydrocaffeoylquinic acid and coumaroyl hexoside) and fifteen flavonols (mostly glycosylated forms of quercetin and kaempferol) were detected. HDFa were pre-incubated with an acerola crude extract (ACExt) and subsequently subjected to oxidative stress induced by AAPH. Apoptosis, intracellular ROS and the biomarkers of lipid and protein oxidation significantly increased after inducing stress, while the activities of the antioxidant enzyme catalase and superoxide dismutase and mitochondrial functionality were markedly affected. However, ACExt was able to protect against oxidative damage through decreasing apoptosis, intracellular ROS levels and lipid and protein damage, besides improving antioxidant enzyme activities and mitochondrial functionality. The obtained results support acerola fruits as relevant sources of functional compounds with promising effects on human health. metadata Alvarez-Suarez, José M.; Giampieri, Francesca; Gasparrini, Massimiliano; Mazzoni, Luca; Santos-Buelga, Celestino; González-Paramás, Ana M.; Forbes-Hernández, Tamara Y.; Afrin, Sadia; Páez-Watson, Timothy; Quiles, José L. y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, tamara.forbes@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2017) The protective effect of acerola (Malpighia emarginata) against oxidative damage in human dermal fibroblasts through the improvement of antioxidant enzyme activity and mitochondrial functionality. Food & Function, 8 (9). pp. 3250-3258. ISSN 2042-6496 relation: http://doi.org/10.1039/c7fo00859g relation: doi:10.1039/c7fo00859g language: en relation: http://repositorio.uneatlantico.es/id/eprint/134/ canonical: http://repositorio.uneatlantico.es/id/eprint/134/ title: Strawberry (cv. Romina) Methanolic Extract and Anthocyanin-Enriched Fraction Improve Lipid Profile and Antioxidant Status in HepG2 Cells creator: Forbes-Hernandez, Tamara Y. creator: Gasparrini, Massimiliano creator: Afrin, Sadia creator: Cianciosi, Danila creator: González-Paramás, Ana creator: Santos-Buelga, Celestino creator: Mezzetti, Bruno creator: Quiles, José L. creator: Battino, Maurizio creator: Giampieri, Francesca creator: Bompadre, Stefano subject: Biomedicina description: Dyslipidemia and oxidation of low density lipoproteins (LDL) are recognized as critical factors in the development of atherosclerosis. Healthy dietary patterns, with abundant fruit and vegetable consumption, may prevent the onset of these risk factors due to the presence of phytochemical compounds. Strawberries are known for their high content of polyphenols; among them, flavonoids are the major constituents, and it is presumed that they are responsible for the biological activity of the fruit. Nevertheless, there are only a few studies that actually evaluate the effects of different fractions isolated from strawberries. In order to assess the effects of two different strawberry extracts (whole methanolic extract/anthocyanin-enriched fraction) on the lipid profile and antioxidant status in human hepatocellular carcinoma (HepG2) cells, the triglycerides and LDL-cholesterol content, lipid peroxidation, intracellular reactive oxygen species (ROS) content and antioxidant enzymes’ activity on cell lysates were determined. Results demonstrated that both strawberry extracts not only improved the lipid metabolism by decreasing triglycerides and LDL-cholesterol contents, but also improved the redox state of HepG2 cells by modulating thiobarbituric acid-reactive substances production, antioxidant enzyme activity and ROS generation. The observed effects were more pronounced for the anthocyanin-enriched fraction. date: 2017 type: Artículo type: PeerReviewed format: text language: en rights: cc_by_4 identifier: http://repositorio.uneatlantico.es/id/eprint/134/1/ijms-18-01149.pdf identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica SIN ESPECIFICAR SIN ESPECIFICAR Dyslipidemia and oxidation of low density lipoproteins (LDL) are recognized as critical factors in the development of atherosclerosis. Healthy dietary patterns, with abundant fruit and vegetable consumption, may prevent the onset of these risk factors due to the presence of phytochemical compounds. Strawberries are known for their high content of polyphenols; among them, flavonoids are the major constituents, and it is presumed that they are responsible for the biological activity of the fruit. Nevertheless, there are only a few studies that actually evaluate the effects of different fractions isolated from strawberries. In order to assess the effects of two different strawberry extracts (whole methanolic extract/anthocyanin-enriched fraction) on the lipid profile and antioxidant status in human hepatocellular carcinoma (HepG2) cells, the triglycerides and LDL-cholesterol content, lipid peroxidation, intracellular reactive oxygen species (ROS) content and antioxidant enzymes’ activity on cell lysates were determined. Results demonstrated that both strawberry extracts not only improved the lipid metabolism by decreasing triglycerides and LDL-cholesterol contents, but also improved the redox state of HepG2 cells by modulating thiobarbituric acid-reactive substances production, antioxidant enzyme activity and ROS generation. The observed effects were more pronounced for the anthocyanin-enriched fraction. metadata Forbes-Hernandez, Tamara Y.; Gasparrini, Massimiliano; Afrin, Sadia; Cianciosi, Danila; González-Paramás, Ana; Santos-Buelga, Celestino; Mezzetti, Bruno; Quiles, José L.; Battino, Maurizio; Giampieri, Francesca y Bompadre, Stefano mail tamara.forbes@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR (2017) Strawberry (cv. Romina) Methanolic Extract and Anthocyanin-Enriched Fraction Improve Lipid Profile and Antioxidant Status in HepG2 Cells. International Journal of Molecular Sciences, 18 (6). p. 1149. ISSN 1422-0067 relation: http://doi.org/10.3390/ijms18061149 relation: doi:10.3390/ijms18061149 relation: http://repositorio.uneatlantico.es/id/eprint/135/ canonical: http://repositorio.uneatlantico.es/id/eprint/135/ title: Lipid Accumulation in HepG2 Cells Is Attenuated by Strawberry Extract through AMPK Activation creator: Forbes-Hernandez, Tamara Y. creator: Giampieri, Francesca creator: Gasparrini, Massimiliano creator: Afrin, Sadia creator: Mazzoni, Luca creator: Cordero, Mario creator: Mezzetti, Bruno creator: Quiles, José L. creator: Battino, Maurizio subject: Biomedicina description: Regulation of lipid metabolism is essential for treatment and prevention of several chronic diseases such as obesity, diabetes, and cardiovascular diseases, which are responsible for most deaths worldwide. It has been demonstrated that the AMP-activated protein kinase (AMPK) has a direct impact on lipid metabolism by modulating several downstream-signaling components. The main objective of the present work was to evaluate the in vitro effect of a methanolic strawberry extract on AMPK and its possible repercussion on lipid metabolism in human hepatocellular carcinoma cells (HepG2). For such purpose, the lipid profile and the expression of proteins metabolically related to AMPK were determined on cells lysates. The results demonstrated that strawberry methanolic extract decreased total cholesterol, low-density lipoprotein (LDL)-cholesterol, and triglycerides levels (up to 0.50-, 0.30-, and 0.40-fold, respectively) while it stimulated the p-AMPK/AMPK expression (up to 3.06-fold), compared to the control. AMPK stimulation led to the phosphorylation and consequent inactivation of acetyl coenzyme A carboxylase (ACC) and inhibition of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), the major regulators of fatty acids and cholesterol synthesis, respectively. Strawberry treatment also entailed a 4.34-, 2.37-, and 2.47-fold overexpression of LDL receptor, sirtuin 1 (Sirt1), and the peroxisome proliferator activated receptor gamma coactivator 1-alpha (PGC-1α), respectively, compared to control. The observed results were counteracted by treatment with compound C, an AMPK pharmacological inhibitor, confirming that multiple effects of strawberries on lipid metabolism are mediated by the activation of this protein. date: 2017 type: Artículo type: PeerReviewed format: text language: en rights: cc_by_4 identifier: http://repositorio.uneatlantico.es/id/eprint/135/1/nutrients-09-00621-v2.pdf identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica SIN ESPECIFICAR SIN ESPECIFICAR Regulation of lipid metabolism is essential for treatment and prevention of several chronic diseases such as obesity, diabetes, and cardiovascular diseases, which are responsible for most deaths worldwide. It has been demonstrated that the AMP-activated protein kinase (AMPK) has a direct impact on lipid metabolism by modulating several downstream-signaling components. The main objective of the present work was to evaluate the in vitro effect of a methanolic strawberry extract on AMPK and its possible repercussion on lipid metabolism in human hepatocellular carcinoma cells (HepG2). For such purpose, the lipid profile and the expression of proteins metabolically related to AMPK were determined on cells lysates. The results demonstrated that strawberry methanolic extract decreased total cholesterol, low-density lipoprotein (LDL)-cholesterol, and triglycerides levels (up to 0.50-, 0.30-, and 0.40-fold, respectively) while it stimulated the p-AMPK/AMPK expression (up to 3.06-fold), compared to the control. AMPK stimulation led to the phosphorylation and consequent inactivation of acetyl coenzyme A carboxylase (ACC) and inhibition of 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), the major regulators of fatty acids and cholesterol synthesis, respectively. Strawberry treatment also entailed a 4.34-, 2.37-, and 2.47-fold overexpression of LDL receptor, sirtuin 1 (Sirt1), and the peroxisome proliferator activated receptor gamma coactivator 1-alpha (PGC-1α), respectively, compared to control. The observed results were counteracted by treatment with compound C, an AMPK pharmacological inhibitor, confirming that multiple effects of strawberries on lipid metabolism are mediated by the activation of this protein. metadata Forbes-Hernandez, Tamara Y.; Giampieri, Francesca; Gasparrini, Massimiliano; Afrin, Sadia; Mazzoni, Luca; Cordero, Mario; Mezzetti, Bruno; Quiles, José L. y Battino, Maurizio mail tamara.forbes@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2017) Lipid Accumulation in HepG2 Cells Is Attenuated by Strawberry Extract through AMPK Activation. Nutrients, 9 (6). p. 621. ISSN 2072-6643 relation: http://doi.org/10.3390/nu9060621 relation: doi:10.3390/nu9060621 relation: http://repositorio.uneatlantico.es/id/eprint/141/ canonical: http://repositorio.uneatlantico.es/id/eprint/141/ title: Strawberry-Based Cosmetic Formulations Protect Human Dermal Fibroblasts against UVA-Induced Damage creator: Gasparrini, Massimiliano creator: Forbes-Hernandez, Tamara Y. creator: Afrin, Sadia creator: Reboredo-Rodriguez, Patricia creator: Cianciosi, Danila creator: Mezzetti, Bruno creator: Quiles, José L. creator: Bompadre, Stefano creator: Battino, Maurizio creator: Giampieri, Francesca subject: Biomedicina description: Extreme exposure of skin to Ultraviolet A (UVA)-radiation may induce a dysregulated production of reactive oxygen species (ROS) which can interact with cellular biomolecules leading to oxidative stress, inflammation, DNA damage, and alteration of cellular molecular pathways, responsible for skin photoaging, hyperplasia, erythema, and cancer. For these reasons, the use of dietary natural bioactive compounds with remarkable antioxidant activity could be a strategic tool to counteract these UVA-radiation-caused deleterious effects. Thus, the purpose of the present work was to test the efficacy of strawberry (50 μg/mL)-based formulations supplemented with Coenzyme Q10 (100 μg/mL) and sun protection factor 10 in human dermal fibroblasts irradiated with UVA-radiation. The apoptosis rate, the amount of intracellular reactive oxygen species (ROS) production, the expression of proteins involved in antioxidant and inflammatory response, and mitochondrial functionality were evaluated. The results showed that the synergic topical use of strawberry and Coenzyme Q10 provided a significant (p < 0.05) photoprotective effect, reducing cell death and ROS, increasing antioxidant defense, lowering inflammatory markers, and improving mitochondrial functionality. The obtained results suggest the use of strawberry-based formulations as an innovative, natural, and useful tool for the prevention of UVA exposure-induced skin diseases in order to decrease or substitute the amount of synthetic sunscreen agents. date: 2017 type: Artículo type: PeerReviewed format: text language: en rights: cc_by_4 identifier: http://repositorio.uneatlantico.es/id/eprint/141/1/nutrients-09-00605.pdf identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica SIN ESPECIFICAR SIN ESPECIFICAR Extreme exposure of skin to Ultraviolet A (UVA)-radiation may induce a dysregulated production of reactive oxygen species (ROS) which can interact with cellular biomolecules leading to oxidative stress, inflammation, DNA damage, and alteration of cellular molecular pathways, responsible for skin photoaging, hyperplasia, erythema, and cancer. For these reasons, the use of dietary natural bioactive compounds with remarkable antioxidant activity could be a strategic tool to counteract these UVA-radiation-caused deleterious effects. Thus, the purpose of the present work was to test the efficacy of strawberry (50 μg/mL)-based formulations supplemented with Coenzyme Q10 (100 μg/mL) and sun protection factor 10 in human dermal fibroblasts irradiated with UVA-radiation. The apoptosis rate, the amount of intracellular reactive oxygen species (ROS) production, the expression of proteins involved in antioxidant and inflammatory response, and mitochondrial functionality were evaluated. The results showed that the synergic topical use of strawberry and Coenzyme Q10 provided a significant (p < 0.05) photoprotective effect, reducing cell death and ROS, increasing antioxidant defense, lowering inflammatory markers, and improving mitochondrial functionality. The obtained results suggest the use of strawberry-based formulations as an innovative, natural, and useful tool for the prevention of UVA exposure-induced skin diseases in order to decrease or substitute the amount of synthetic sunscreen agents. metadata Gasparrini, Massimiliano; Forbes-Hernandez, Tamara Y.; Afrin, Sadia; Reboredo-Rodriguez, Patricia; Cianciosi, Danila; Mezzetti, Bruno; Quiles, José L.; Bompadre, Stefano; Battino, Maurizio y Giampieri, Francesca mail SIN ESPECIFICAR, tamara.forbes@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es, SIN ESPECIFICAR (2017) Strawberry-Based Cosmetic Formulations Protect Human Dermal Fibroblasts against UVA-Induced Damage. Nutrients, 9 (6). p. 605. ISSN 2072-6643 relation: http://doi.org/10.3390/nu9060605 relation: doi:10.3390/nu9060605 relation: http://repositorio.uneatlantico.es/id/eprint/142/ canonical: http://repositorio.uneatlantico.es/id/eprint/142/ title: Anti-inflammatory effect of strawberry extract against LPS-induced stress in RAW 264.7 macrophages creator: Gasparrini, Massimiliano creator: Forbes-Hernandez, Tamara Y. creator: Giampieri, Francesca creator: Afrin, Sadia creator: Alvarez-Suarez, Josè M. creator: Mazzoni, Luca creator: Mezzetti, Bruno creator: Quiles, Josè L. creator: Battino, Maurizio subject: Biomedicina description: A common denominator in the pathogenesis of most chronic inflammatory diseases is the involvement of oxidative stress, related to ROS production by all aerobic organisms. Dietary antioxidants from plant foods represent an efficient strategy to counteract this condition. The aim of the present study was to evaluate the protective effects of strawberry extracts on inflammatory status induced by E. Coli LPS on RAW 264.7 macrophages by measuring the main oxidative and inflammatory biomarkers and investigating the molecular pathways involved. Strawberry pre-treatment efficiently counteracted LPS-induced oxidative stress reducing the amount of ROS and nitrite production, stimulating endogenous antioxidant enzyme activities and enhancing protection against lipid, protein and DNA damage (P < 0.05). Strawberry pre-treatment exerted these protective effects primarily through the activation of the Nrf2 pathway, which is markedly AMPK-dependent and also by the modulation of the NF-kB signalling pathway. Finally, an improvement in mitochondria functionality was also detected. The results obtained in this work highlight the health benefit of strawberries against inflammatory and oxidative stress in LPS-stimulated RAW 264.7 macrophages, investigating for the first time the possible involved molecular mechanisms. date: 2017 type: Artículo type: PeerReviewed identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica Cerrado Inglés A common denominator in the pathogenesis of most chronic inflammatory diseases is the involvement of oxidative stress, related to ROS production by all aerobic organisms. Dietary antioxidants from plant foods represent an efficient strategy to counteract this condition. The aim of the present study was to evaluate the protective effects of strawberry extracts on inflammatory status induced by E. Coli LPS on RAW 264.7 macrophages by measuring the main oxidative and inflammatory biomarkers and investigating the molecular pathways involved. Strawberry pre-treatment efficiently counteracted LPS-induced oxidative stress reducing the amount of ROS and nitrite production, stimulating endogenous antioxidant enzyme activities and enhancing protection against lipid, protein and DNA damage (P < 0.05). Strawberry pre-treatment exerted these protective effects primarily through the activation of the Nrf2 pathway, which is markedly AMPK-dependent and also by the modulation of the NF-kB signalling pathway. Finally, an improvement in mitochondria functionality was also detected. The results obtained in this work highlight the health benefit of strawberries against inflammatory and oxidative stress in LPS-stimulated RAW 264.7 macrophages, investigating for the first time the possible involved molecular mechanisms. metadata Gasparrini, Massimiliano; Forbes-Hernandez, Tamara Y.; Giampieri, Francesca; Afrin, Sadia; Alvarez-Suarez, Josè M.; Mazzoni, Luca; Mezzetti, Bruno; Quiles, Josè L. y Battino, Maurizio mail SIN ESPECIFICAR, tamara.forbes@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2017) Anti-inflammatory effect of strawberry extract against LPS-induced stress in RAW 264.7 macrophages. Food and Chemical Toxicology, 102. pp. 1-10. ISSN 0278-6915 relation: http://doi.org/10.1016/j.fct.2017.01.018 relation: doi:10.1016/j.fct.2017.01.018 language: en relation: http://repositorio.uneatlantico.es/id/eprint/143/ canonical: http://repositorio.uneatlantico.es/id/eprint/143/ title: Protective Effect of Strawberry Extract against Inflammatory Stress Induced in Human Dermal Fibroblasts creator: Gasparrini, Massimiliano creator: Forbes-Hernandez, Tamara Y. creator: Giampieri, Francesca creator: Afrin, Sadia creator: Mezzetti, Bruno creator: Quiles, José L. creator: Bompadre, Stefano creator: Battino, Maurizio subject: Biomedicina description: A protracted pro-inflammatory state is a major contributing factor in the development, progression and complication of the most common chronic pathologies. Fruit and vegetables represent the main sources of dietary antioxidants and their consumption can be considered an efficient tool to counteract inflammatory states. In this context an evaluation of the protective effects of strawberry extracts on inflammatory stress induced by E. coli LPS on human dermal fibroblast cells was performed in terms of viability assays, ROS and nitrite production and biomarkers of oxidative damage of the main biological macromolecules. The results demonstrated that strawberry extracts exerted an anti-inflammatory effect on LPS-treated cells, through an increase in cell viability, and the reduction of ROS and nitrite levels, and lipid, protein and DNA damage. This work showed for the first time the potential health benefits of strawberry extract against inflammatory and oxidative stress in LPS-treated human dermal fibroblast cells. date: 2017 type: Artículo type: PeerReviewed format: text language: en rights: cc_by_4 identifier: http://repositorio.uneatlantico.es/id/eprint/143/1/molecules-22-00164.pdf identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica SIN ESPECIFICAR SIN ESPECIFICAR A protracted pro-inflammatory state is a major contributing factor in the development, progression and complication of the most common chronic pathologies. Fruit and vegetables represent the main sources of dietary antioxidants and their consumption can be considered an efficient tool to counteract inflammatory states. In this context an evaluation of the protective effects of strawberry extracts on inflammatory stress induced by E. coli LPS on human dermal fibroblast cells was performed in terms of viability assays, ROS and nitrite production and biomarkers of oxidative damage of the main biological macromolecules. The results demonstrated that strawberry extracts exerted an anti-inflammatory effect on LPS-treated cells, through an increase in cell viability, and the reduction of ROS and nitrite levels, and lipid, protein and DNA damage. This work showed for the first time the potential health benefits of strawberry extract against inflammatory and oxidative stress in LPS-treated human dermal fibroblast cells. metadata Gasparrini, Massimiliano; Forbes-Hernandez, Tamara Y.; Giampieri, Francesca; Afrin, Sadia; Mezzetti, Bruno; Quiles, José L.; Bompadre, Stefano y Battino, Maurizio mail SIN ESPECIFICAR, tamara.forbes@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2017) Protective Effect of Strawberry Extract against Inflammatory Stress Induced in Human Dermal Fibroblasts. Molecules, 22 (1). p. 164. ISSN 1420-3049 relation: http://doi.org/10.3390/molecules22010164 relation: doi:10.3390/molecules22010164 relation: http://repositorio.uneatlantico.es/id/eprint/89/ canonical: http://repositorio.uneatlantico.es/id/eprint/89/ title: Data on body weight and liver functionality in aged rats fed an enriched strawberry diet creator: Giampieri, Francesca creator: Alvarez-Suarez, Josè M. creator: Gasparrini, Massimiliano creator: Forbes-Hernandez, Tamara Y. creator: Afrin, Sadia creator: Rubini, Corrado creator: Zizzi, Antonio creator: Quiles, Josè L. creator: Mezzetti, Bruno creator: Battino, Maurizio subject: Alimentación description: Here, we present new original data on the effects of strawberry consumption on body weight and liver status of aged rats. Wistar rats aged 19–21 months were fed a strawberry enriched diet prepared by substituting 15% of the total calories with freeze-dried strawberry powder for two months. Body weight, plasma biomarkers of liver injury (alanine transferase, aspartate aminotransferase and alkaline phosphatase) and liver histological analysis were assessed. These data indicate that strawberry supplementation did not interfere with normal animal maintenance and with liver structure and functionality. For further details and experimental findings please refer to the article “Strawberry consumption improves aging-associated impairments, mitochondrial biogenesis and functionality through the AMP-Activated Protein Kinase signaling cascade” in FOOD CHEMISTRY date: 2017 type: Artículo type: PeerReviewed identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros SIN ESPECIFICAR SIN ESPECIFICAR Here, we present new original data on the effects of strawberry consumption on body weight and liver status of aged rats. Wistar rats aged 19–21 months were fed a strawberry enriched diet prepared by substituting 15% of the total calories with freeze-dried strawberry powder for two months. Body weight, plasma biomarkers of liver injury (alanine transferase, aspartate aminotransferase and alkaline phosphatase) and liver histological analysis were assessed. These data indicate that strawberry supplementation did not interfere with normal animal maintenance and with liver structure and functionality. For further details and experimental findings please refer to the article “Strawberry consumption improves aging-associated impairments, mitochondrial biogenesis and functionality through the AMP-Activated Protein Kinase signaling cascade” in FOOD CHEMISTRY metadata Giampieri, Francesca; Alvarez-Suarez, Josè M.; Gasparrini, Massimiliano; Forbes-Hernandez, Tamara Y.; Afrin, Sadia; Rubini, Corrado; Zizzi, Antonio; Quiles, Josè L.; Mezzetti, Bruno y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2017) Data on body weight and liver functionality in aged rats fed an enriched strawberry diet. Data in Brief, 13. pp. 432-436. ISSN 2352-3409 relation: http://doi.org/10.1016/j.dib.2017.06.021 relation: doi:10.1016/j.dib.2017.06.021 relation: http://repositorio.uneatlantico.es/id/eprint/144/ canonical: http://repositorio.uneatlantico.es/id/eprint/144/ title: The healthy effects of strawberry bioactive compounds on molecular pathways related to chronic diseases creator: Giampieri, Francesca creator: Forbes-Hernandez, Tamara Y. creator: Gasparrini, Massimiliano creator: Afrin, Sadia creator: Cianciosi, Danila creator: Reboredo-Rodriguez, Patricia creator: Varela-Lopez, Alfonso creator: Quiles, Jose L. creator: Mezzetti, Bruno creator: Battino, Maurizio subject: Biomedicina description: It is generally accepted that a fruit and vegetable–enriched diet is favorable for human health. The consumption of strawberries, in particular, has been related to the maintenance of well-being and the prevention of several chronic diseases, owing to the high contents of antioxidants and phytochemicals present in the fruit. Several biological effects have been explained through the total antioxidant capacity exerted by these bioactive compounds, but recently more intricate mechanisms have begun to be examined. In this context, it has been reported that strawberry phenolics are able to exert anti-inflammatory, anticarcinogenic, antiproliferative, and antiatherosclerotic activities, acting on specific molecular pathways related to antioxidant defenses, metabolism, survival, and proliferation. The overall aim of this work is to discuss and update the cellular and molecular mechanisms recently proposed to clarify the effects of strawberry phenolics on human health, with particular attention to the most common chronic diseases, such as metabolic syndrome, cardiovascular disease, and cancer. date: 2017 type: Artículo type: PeerReviewed identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica Cerrado Inglés It is generally accepted that a fruit and vegetable–enriched diet is favorable for human health. The consumption of strawberries, in particular, has been related to the maintenance of well-being and the prevention of several chronic diseases, owing to the high contents of antioxidants and phytochemicals present in the fruit. Several biological effects have been explained through the total antioxidant capacity exerted by these bioactive compounds, but recently more intricate mechanisms have begun to be examined. In this context, it has been reported that strawberry phenolics are able to exert anti-inflammatory, anticarcinogenic, antiproliferative, and antiatherosclerotic activities, acting on specific molecular pathways related to antioxidant defenses, metabolism, survival, and proliferation. The overall aim of this work is to discuss and update the cellular and molecular mechanisms recently proposed to clarify the effects of strawberry phenolics on human health, with particular attention to the most common chronic diseases, such as metabolic syndrome, cardiovascular disease, and cancer. metadata Giampieri, Francesca; Forbes-Hernandez, Tamara Y.; Gasparrini, Massimiliano; Afrin, Sadia; Cianciosi, Danila; Reboredo-Rodriguez, Patricia; Varela-Lopez, Alfonso; Quiles, Jose L.; Mezzetti, Bruno y Battino, Maurizio mail SIN ESPECIFICAR, tamara.forbes@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2017) The healthy effects of strawberry bioactive compounds on molecular pathways related to chronic diseases. Annals of the New York Academy of Sciences, 1398 (1). pp. 62-71. ISSN 0077-8923 relation: http://doi.org/10.1111/nyas.13373 relation: doi:10.1111/nyas.13373 language: en relation: http://repositorio.uneatlantico.es/id/eprint/145/ canonical: http://repositorio.uneatlantico.es/id/eprint/145/ title: The use of natural compounds for the targeting and chemoprevention of ovarian cancer creator: Pistollato, Francesca creator: Calderón Iglesias, Rubén creator: Ruiz Salces, Roberto creator: Aparicio Obregón, Silvia creator: Crespo Alvare, Jorge creator: Dzul Lopez, Luis creator: Giampieri, Francesca creator: Battino, Maurizio subject: Biomedicina description: Among gynaecological cancers, ovarian cancer represents the leading cause of death in women. Current treatment for ovarian cancer entails surgery followed by combined chemotherapy with platinum and taxane, which are associated, particularly cisplatin, with severe side effects. While this treatment approach appears to be initially effective in a high number of patients, nearly 70% of them suffer a relapse within a few months after initial treatment. Therefore, more effective and better-tolerated treatment options are clearly needed. In recent years, several natural compounds (such as curcumin, epigallocatechin 3-gallate (EGCG), resveratrol, sulforaphane and Withaferin-A), characterized by long-term safety and negligible and/or inexistent side effects, have been proposed as possible adjuvants of traditional chemotherapy. Indeed, several in vitro and in vivo studies have shown that phytocompounds can effectively inhibit tumor cell proliferation, stimulate autophagy, induce apoptosis, and specifically target ovarian cancer stem cells (CSCs), which are generally considered to be responsible for tumor recurrence in several types of cancer. Here we review current literature on the role of natural products in ovarian cancer chemoprevention, highlighting their effects particularly on the regulation of inflammation, autophagy, proliferation and apoptosis, chemotherapy resistance, and ovarian CSC growth. date: 2017 type: Artículo type: PeerReviewed identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica Cerrado Inglés Among gynaecological cancers, ovarian cancer represents the leading cause of death in women. Current treatment for ovarian cancer entails surgery followed by combined chemotherapy with platinum and taxane, which are associated, particularly cisplatin, with severe side effects. While this treatment approach appears to be initially effective in a high number of patients, nearly 70% of them suffer a relapse within a few months after initial treatment. Therefore, more effective and better-tolerated treatment options are clearly needed. In recent years, several natural compounds (such as curcumin, epigallocatechin 3-gallate (EGCG), resveratrol, sulforaphane and Withaferin-A), characterized by long-term safety and negligible and/or inexistent side effects, have been proposed as possible adjuvants of traditional chemotherapy. Indeed, several in vitro and in vivo studies have shown that phytocompounds can effectively inhibit tumor cell proliferation, stimulate autophagy, induce apoptosis, and specifically target ovarian cancer stem cells (CSCs), which are generally considered to be responsible for tumor recurrence in several types of cancer. Here we review current literature on the role of natural products in ovarian cancer chemoprevention, highlighting their effects particularly on the regulation of inflammation, autophagy, proliferation and apoptosis, chemotherapy resistance, and ovarian CSC growth. metadata Pistollato, Francesca; Calderón Iglesias, Rubén; Ruiz Salces, Roberto; Aparicio Obregón, Silvia; Crespo Alvare, Jorge; Dzul Lopez, Luis; Giampieri, Francesca y Battino, Maurizio mail SIN ESPECIFICAR, ruben.calderon@uneatlantico.es, roberto.ruiz@uneatlantico.es, silvia.aparicio@uneatlantico.es, jorge.crespo@uneatlantico.es, luis.dzul@unini.edu.mx, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2017) The use of natural compounds for the targeting and chemoprevention of ovarian cancer. Cancer Letters, 411. pp. 191-200. ISSN 0304-3835 relation: http://doi.org/10.1016/j.canlet.2017.09.050 relation: doi:10.1016/j.canlet.2017.09.050 language: en relation: http://repositorio.uneatlantico.es/id/eprint/90/ canonical: http://repositorio.uneatlantico.es/id/eprint/90/ title: Hepatoprotective effects of raspberry (Rubus coreanus Miq.) seed oil and its major constituents creator: Teng, Hui creator: Lin, Qiyang creator: Li, Kang creator: Yuan, Benyao creator: Song, Hongbo creator: Peng, Hongquan creator: Yi, Lunzhao creator: Wei, Ming-Chi creator: Yang, Yu-Chiao creator: Battino, Maurizio creator: Cespedes Acuña, Carlos L. creator: Chen, Lei creator: Xiao, Jianbo subject: Alimentación description: Raspberry seed is a massive byproduct of raspberry juice and wine but usually discarded. The present study employed a microwave-assisted method for extraction of raspberry seed oil (RSO). The results revealed that omega-6 fatty acids (linoleic acid and γ-linolenic acid) were the major constituents in RSO. Cellular antioxidant enzyme activity such as superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were investigated in HepG2 cells treated with RSO. Induction of the synthesis of several antioxidants in H2O2-exposed HepG2 cells was found. RSO increased the enzyme activity of SOD, CAT, and GPx in H2O2-exposed HepG2. Furthermore, RSO inhibited the phosphorylation of upstream mitogen-activated protein kinases (MAPK) such as c-Jun N-terminal kinase (c-JNK) and extracellular signal-regulated kinase (ERK). Taken together, the possible mechanisms to increase antioxidant enzyme activities in HepG2 may through the suppression of ERK and JNK phosphorylation. Raspberry seed oil exhibited good effects on the activities of the intracellular antioxidant enzymes and seems to protect the liver from oxidative stress through the inhibition of MAPKs. date: 2017 type: Artículo type: PeerReviewed identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros SIN ESPECIFICAR SIN ESPECIFICAR Raspberry seed is a massive byproduct of raspberry juice and wine but usually discarded. The present study employed a microwave-assisted method for extraction of raspberry seed oil (RSO). The results revealed that omega-6 fatty acids (linoleic acid and γ-linolenic acid) were the major constituents in RSO. Cellular antioxidant enzyme activity such as superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were investigated in HepG2 cells treated with RSO. Induction of the synthesis of several antioxidants in H2O2-exposed HepG2 cells was found. RSO increased the enzyme activity of SOD, CAT, and GPx in H2O2-exposed HepG2. Furthermore, RSO inhibited the phosphorylation of upstream mitogen-activated protein kinases (MAPK) such as c-Jun N-terminal kinase (c-JNK) and extracellular signal-regulated kinase (ERK). Taken together, the possible mechanisms to increase antioxidant enzyme activities in HepG2 may through the suppression of ERK and JNK phosphorylation. Raspberry seed oil exhibited good effects on the activities of the intracellular antioxidant enzymes and seems to protect the liver from oxidative stress through the inhibition of MAPKs. metadata Teng, Hui; Lin, Qiyang; Li, Kang; Yuan, Benyao; Song, Hongbo; Peng, Hongquan; Yi, Lunzhao; Wei, Ming-Chi; Yang, Yu-Chiao; Battino, Maurizio; Cespedes Acuña, Carlos L.; Chen, Lei y Xiao, Jianbo mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2017) Hepatoprotective effects of raspberry (Rubus coreanus Miq.) seed oil and its major constituents. Food and Chemical Toxicology, 110. pp. 418-424. ISSN 0278-6915 relation: http://doi.org/10.1016/j.fct.2017.09.010 relation: doi:10.1016/j.fct.2017.09.010 relation: http://repositorio.uneatlantico.es/id/eprint/91/ canonical: http://repositorio.uneatlantico.es/id/eprint/91/ title: Promising Health Benefits of the Strawberry: A Focus on Clinical Studies creator: Afrin, Sadia creator: Gasparrini, Massimiliano creator: Forbes-Hernandez, Tamara Y. creator: Reboredo-Rodriguez, Patricia creator: Mezzetti, Bruno creator: Varela-López, Alfonso creator: Giampieri, Francesca creator: Battino, Maurizio subject: Alimentación description: The potential health benefits associated with dietary intake of fruits have attracted increasing interest. Among berries, the strawberry is a rich source of several nutritive and non-nutritive bioactive compounds, which are implicated in various health-promoting and disease preventive effects. A plethora of studies have examined the benefits of strawberry consumption, such as prevention of inflammation disorders and oxidative stress, reduction of obesity related disorders and heart disease risk, and protection against various types of cancer. This review provides an overview of their nutritional and non-nutritional bioactive compounds and which factors affect their content in strawberries. In addition, the bioavailability and metabolism of major strawberry phytochemicals as well as their actions in combating many pathologies, including cancer, metabolic syndrome, cardiovascular disease, obesity, diabetes, neurodegeneration, along with microbial pathogenesis have been reviewed, with a particular attention to human studies. date: 2016 type: Artículo type: PeerReviewed identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros SIN ESPECIFICAR SIN ESPECIFICAR The potential health benefits associated with dietary intake of fruits have attracted increasing interest. Among berries, the strawberry is a rich source of several nutritive and non-nutritive bioactive compounds, which are implicated in various health-promoting and disease preventive effects. A plethora of studies have examined the benefits of strawberry consumption, such as prevention of inflammation disorders and oxidative stress, reduction of obesity related disorders and heart disease risk, and protection against various types of cancer. This review provides an overview of their nutritional and non-nutritional bioactive compounds and which factors affect their content in strawberries. In addition, the bioavailability and metabolism of major strawberry phytochemicals as well as their actions in combating many pathologies, including cancer, metabolic syndrome, cardiovascular disease, obesity, diabetes, neurodegeneration, along with microbial pathogenesis have been reviewed, with a particular attention to human studies. metadata Afrin, Sadia; Gasparrini, Massimiliano; Forbes-Hernandez, Tamara Y.; Reboredo-Rodriguez, Patricia; Mezzetti, Bruno; Varela-López, Alfonso; Giampieri, Francesca y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2016) Promising Health Benefits of the Strawberry: A Focus on Clinical Studies. Journal of Agricultural and Food Chemistry, 64 (22). pp. 4435-4449. ISSN 0021-8561 relation: http://doi.org/10.1021/acs.jafc.6b00857 relation: doi:10.1021/acs.jafc.6b00857 relation: http://repositorio.uneatlantico.es/id/eprint/146/ canonical: http://repositorio.uneatlantico.es/id/eprint/146/ title: Chemopreventive and Therapeutic Effects of Edible Berries: A Focus on Colon Cancer Prevention and Treatment creator: Afrin, Sadia creator: Giampieri, Francesca creator: Gasparrini, Massimiliano creator: Forbes-Hernandez, Tamara creator: Varela-López, Alfonso creator: Quiles, José creator: Mezzetti, Bruno creator: Battino, Maurizio subject: Biomedicina description: Colon cancer is one of the most prevalent diseases across the world. Numerous epidemiological studies indicate that diets rich in fruit, such as berries, provide significant health benefits against several types of cancer, including colon cancer. The anticancer activities of berries are attributed to their high content of phytochemicals and to their relevant antioxidant properties. In vitro and in vivo studies have demonstrated that berries and their bioactive components exert therapeutic and preventive effects against colon cancer by the suppression of inflammation, oxidative stress, proliferation and angiogenesis, through the modulation of multiple signaling pathways such as NF-κB, Wnt/β-catenin, PI3K/AKT/PKB/mTOR, and ERK/MAPK. Based on the exciting outcomes of preclinical studies, a few berries have advanced to the clinical phase. A limited number of human studies have shown that consumption of berries can prevent colorectal cancer, especially in patients at high risk (familial adenopolyposis or aberrant crypt foci, and inflammatory bowel diseases). In this review, we aim to highlight the findings of berries and their bioactive compounds in colon cancer from in vitro and in vivo studies, both on animals and humans. Thus, this review could be a useful step towards the next phase of berry research in colon cancer. date: 2016 type: Artículo type: PeerReviewed format: text language: en rights: cc_by_4 identifier: http://repositorio.uneatlantico.es/id/eprint/146/1/molecules-21-00169.pdf identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros SIN ESPECIFICAR SIN ESPECIFICAR Colon cancer is one of the most prevalent diseases across the world. Numerous epidemiological studies indicate that diets rich in fruit, such as berries, provide significant health benefits against several types of cancer, including colon cancer. The anticancer activities of berries are attributed to their high content of phytochemicals and to their relevant antioxidant properties. In vitro and in vivo studies have demonstrated that berries and their bioactive components exert therapeutic and preventive effects against colon cancer by the suppression of inflammation, oxidative stress, proliferation and angiogenesis, through the modulation of multiple signaling pathways such as NF-κB, Wnt/β-catenin, PI3K/AKT/PKB/mTOR, and ERK/MAPK. Based on the exciting outcomes of preclinical studies, a few berries have advanced to the clinical phase. A limited number of human studies have shown that consumption of berries can prevent colorectal cancer, especially in patients at high risk (familial adenopolyposis or aberrant crypt foci, and inflammatory bowel diseases). In this review, we aim to highlight the findings of berries and their bioactive compounds in colon cancer from in vitro and in vivo studies, both on animals and humans. Thus, this review could be a useful step towards the next phase of berry research in colon cancer. metadata Afrin, Sadia; Giampieri, Francesca; Gasparrini, Massimiliano; Forbes-Hernandez, Tamara; Varela-López, Alfonso; Quiles, José; Mezzetti, Bruno y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2016) Chemopreventive and Therapeutic Effects of Edible Berries: A Focus on Colon Cancer Prevention and Treatment. Molecules, 21 (2). p. 169. ISSN 1420-3049 relation: http://doi.org/10.3390/molecules21020169 relation: doi:10.3390/molecules21020169 relation: http://repositorio.uneatlantico.es/id/eprint/176/ canonical: http://repositorio.uneatlantico.es/id/eprint/176/ title: Activation of AMPK/Nrf2 signalling by Manuka honey protects human dermal fibroblasts against oxidative damage by improving antioxidant response and mitochondrial function promoting wound healing creator: Alvarez-Suarez, José Miguel creator: Giampieri, Francesca creator: Cordero, Mario creator: Gasparrini, Massimiliano creator: Forbes-Hernandez, Tamara Yuliet creator: Mazzoni, Luca creator: Afrin, Sadia creator: Beltrán-Ayala, Pablo creator: González-Paramás, Ana María creator: Santos-Buelga, Celestino creator: Varela-Lopez, Alfonso creator: Quiles, José Luis creator: Battino, Maurizio subject: Biomedicina description: Excessive amounts of free radicals are deleterious for cells, resulting in cell damage, affecting the wound healing process and causing premature ageing or even neoplastic transformation. Here the capacity of Manuka honey (MH) to protect against oxidative damage and improve the process of skin wound healing was investigated. Up to 16 compounds were identified in MH, with leptosin derivatives and methyl syringate as the major ones. MH protected against apoptosis, intracellular ROS production, and lipid and protein oxidative damage. MH also protected mitochondrial functionality, promoted cell proliferation and activated the AMPK/Nrf2/ARE signalling pathway, as well as the expression of the antioxidant enzymes such as SOD and CAT. Here we describe for the first time that one of the possible mechanisms by which MH exhibits its ability to promote wound healing could be due to its capacity to improve the antioxidant response by activating AMPK phosphorylation and the ARE response. publisher: ELSEVIER date: 2016 type: Artículo type: PeerReviewed identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Cerrado Inglés Excessive amounts of free radicals are deleterious for cells, resulting in cell damage, affecting the wound healing process and causing premature ageing or even neoplastic transformation. Here the capacity of Manuka honey (MH) to protect against oxidative damage and improve the process of skin wound healing was investigated. Up to 16 compounds were identified in MH, with leptosin derivatives and methyl syringate as the major ones. MH protected against apoptosis, intracellular ROS production, and lipid and protein oxidative damage. MH also protected mitochondrial functionality, promoted cell proliferation and activated the AMPK/Nrf2/ARE signalling pathway, as well as the expression of the antioxidant enzymes such as SOD and CAT. Here we describe for the first time that one of the possible mechanisms by which MH exhibits its ability to promote wound healing could be due to its capacity to improve the antioxidant response by activating AMPK phosphorylation and the ARE response. metadata Alvarez-Suarez, José Miguel; Giampieri, Francesca; Cordero, Mario; Gasparrini, Massimiliano; Forbes-Hernandez, Tamara Yuliet; Mazzoni, Luca; Afrin, Sadia; Beltrán-Ayala, Pablo; González-Paramás, Ana María; Santos-Buelga, Celestino; Varela-Lopez, Alfonso; Quiles, José Luis y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, tamara.forbes@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2016) Activation of AMPK/Nrf2 signalling by Manuka honey protects human dermal fibroblasts against oxidative damage by improving antioxidant response and mitochondrial function promoting wound healing. Journal of Functional Foods, 25. pp. 38-49. ISSN 1756-4646 relation: http://doi.org/10.1016/j.jff.2016.05.008 relation: doi:10.1016/j.jff.2016.05.008 language: en relation: http://repositorio.uneatlantico.es/id/eprint/92/ canonical: http://repositorio.uneatlantico.es/id/eprint/92/ title: Polyphenol-rich strawberry extract (PRSE) shows in vitro and in vivo biological activity against invasive breast cancer cells creator: Amatori, Stefano creator: Mazzoni, Luca creator: Alvarez-Suarez, José M. creator: Giampieri, Francesca creator: Gasparrini, Massimiliano creator: Forbes-Hernandez, Tamara Y. creator: Afrin, Sadia creator: Errico Provenzano, Alfredo creator: Persico, Giuseppe creator: Mezzetti, Bruno creator: Amici, Augusto creator: Fanelli, Mirco creator: Battino, Maurizio subject: Alimentación description: We describe the biological effects of a polyphenol-rich strawberry extract (PRSE), obtained from the “Alba” variety, on the highly aggressive and invasive basal-like breast cancer cell line A17. Dose-response and time-course experiments showed that PRSE is able to decrease the cellular viability of A17 cells in a time- and dose-dependent manner. PRSE effect on cell survival was investigated in other tumor and normal cell lines of both mouse and human origin, demonstrating that PRSE is more active against breast cancer cells. Cytofluorimetric analysis of A17 cells demonstrated that sub-lethal doses of PRSE reduce the number of cells in S phase, inducing the accumulation of cells in G1 phase of cell cycle. In addition, the migration of A17 cells was studied monitoring the ability of PRSE to inhibit cellular mobility. Gene expression analysis revealed the modulation of 12 genes playing different roles in the cellular migration, adhesion and invasion processes. Finally, in vivo experiments showed the growth inhibition of A17 cells orthotopically transplanted into FVB syngeneic mice fed with PRSE. Overall, we demonstrated that PRSE exerts important biological activities against a highly invasive breast cancer cell line both in vitro and in vivo suggesting the strawberry extracts as preventive/curative food strategy. date: 2016 type: Artículo type: PeerReviewed format: text language: en rights: cc_by_4 identifier: http://repositorio.uneatlantico.es/id/eprint/92/1/srep30917.pdf identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica SIN ESPECIFICAR SIN ESPECIFICAR We describe the biological effects of a polyphenol-rich strawberry extract (PRSE), obtained from the “Alba” variety, on the highly aggressive and invasive basal-like breast cancer cell line A17. Dose-response and time-course experiments showed that PRSE is able to decrease the cellular viability of A17 cells in a time- and dose-dependent manner. PRSE effect on cell survival was investigated in other tumor and normal cell lines of both mouse and human origin, demonstrating that PRSE is more active against breast cancer cells. Cytofluorimetric analysis of A17 cells demonstrated that sub-lethal doses of PRSE reduce the number of cells in S phase, inducing the accumulation of cells in G1 phase of cell cycle. In addition, the migration of A17 cells was studied monitoring the ability of PRSE to inhibit cellular mobility. Gene expression analysis revealed the modulation of 12 genes playing different roles in the cellular migration, adhesion and invasion processes. Finally, in vivo experiments showed the growth inhibition of A17 cells orthotopically transplanted into FVB syngeneic mice fed with PRSE. Overall, we demonstrated that PRSE exerts important biological activities against a highly invasive breast cancer cell line both in vitro and in vivo suggesting the strawberry extracts as preventive/curative food strategy. metadata Amatori, Stefano; Mazzoni, Luca; Alvarez-Suarez, José M.; Giampieri, Francesca; Gasparrini, Massimiliano; Forbes-Hernandez, Tamara Y.; Afrin, Sadia; Errico Provenzano, Alfredo; Persico, Giuseppe; Mezzetti, Bruno; Amici, Augusto; Fanelli, Mirco y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, tamara.forbes@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2016) Polyphenol-rich strawberry extract (PRSE) shows in vitro and in vivo biological activity against invasive breast cancer cells. Scientific Reports, 6 (1). ISSN 2045-2322 relation: http://doi.org/10.1038/srep30917 relation: doi:10.1038/srep30917 relation: http://repositorio.uneatlantico.es/id/eprint/93/ canonical: http://repositorio.uneatlantico.es/id/eprint/93/ title: The Healthy Effects of Strawberry Polyphenols: Which Strategy behind Antioxidant Capacity? creator: Forbes-Hernandez, Tamara Y. creator: Gasparrini, Massimiliano creator: Afrin, Sadia creator: Bompadre, Stefano creator: Mezzetti, Bruno creator: Quiles, Josè L. creator: Giampieri, Francesca creator: Battino, Maurizio subject: Alimentación description: Current evidence indicates that the consumption of strawberries, a natural source of a wide range of nutritive and bioactive compounds, is associated with the prevention and improvement of chronic-degenerative diseases.. Studies involving cells and animals provide evidence on the anti-inflammatory, anticarcinogenic and antiproliferative activity of the strawberry. Epidemiological and clinical studies demonstrate that its acute consumption increases plasma antioxidant capacity, improves circulating inflammatory markers and ameliorates postprandial glycemic response. At the same time, a protracted intake reduces chronic inflammation and improves plasma lipid profile, supporting cardiovascular health, especially in individuals with increased risk for metabolic syndrome. To explain these beneficial effects, much attention has been paid in the past to the antioxidant properties of strawberry polyphenols. However, recent research has shown that their biological and functional activities are related not only to the antioxidant capacity but also to the modulation of many cellular pathways involved in metabolism, survival, proliferation, and antioxidant defenses. The aim of this review is to update and discuss the molecular and cellular mechanisms proposed in recent studies to elucidate the healthy effects of strawberry polyphenols against the most common chronic diseases, such as cancer, cardiovascular diseases, metabolic syndrome, and inflammation. date: 2016-07-08 type: Artículo type: PeerReviewed identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica Cerrado Inglés Current evidence indicates that the consumption of strawberries, a natural source of a wide range of nutritive and bioactive compounds, is associated with the prevention and improvement of chronic-degenerative diseases.. Studies involving cells and animals provide evidence on the anti-inflammatory, anticarcinogenic and antiproliferative activity of the strawberry. Epidemiological and clinical studies demonstrate that its acute consumption increases plasma antioxidant capacity, improves circulating inflammatory markers and ameliorates postprandial glycemic response. At the same time, a protracted intake reduces chronic inflammation and improves plasma lipid profile, supporting cardiovascular health, especially in individuals with increased risk for metabolic syndrome. To explain these beneficial effects, much attention has been paid in the past to the antioxidant properties of strawberry polyphenols. However, recent research has shown that their biological and functional activities are related not only to the antioxidant capacity but also to the modulation of many cellular pathways involved in metabolism, survival, proliferation, and antioxidant defenses. The aim of this review is to update and discuss the molecular and cellular mechanisms proposed in recent studies to elucidate the healthy effects of strawberry polyphenols against the most common chronic diseases, such as cancer, cardiovascular diseases, metabolic syndrome, and inflammation. metadata Forbes-Hernandez, Tamara Y.; Gasparrini, Massimiliano; Afrin, Sadia; Bompadre, Stefano; Mezzetti, Bruno; Quiles, Josè L.; Giampieri, Francesca y Battino, Maurizio mail tamara.forbes@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, francesca.giampieri@uneatlantico.es, maurizio.battino@uneatlantico.es (2016) The Healthy Effects of Strawberry Polyphenols: Which Strategy behind Antioxidant Capacity? Critical Reviews in Food Science and Nutrition, 56 (sup1). S46-S59. ISSN 1040-8398 relation: http://doi.org/10.1080/10408398.2015.1051919 relation: doi:10.1080/10408398.2015.1051919 language: en relation: http://repositorio.uneatlantico.es/id/eprint/147/ canonical: http://repositorio.uneatlantico.es/id/eprint/147/ title: Strawberry consumption alleviates doxorubicin-induced toxicity by suppressing oxidative stress creator: Giampieri, Francesca creator: Alvarez-Suarez, Jose M. creator: Gasparrini, Massimiliano creator: Forbes- Hernandez, Tamara Y. creator: Afrin, Sadia creator: Bompadre, Stefano creator: Rubini, Corrado creator: Zizzi, Antonio creator: Astolfi, Paola creator: Santos-Buelga, Celestino creator: González-Paramás, Ana M. creator: Quiles, Josè L. creator: Mezzetti, Bruno creator: Battino, Maurizio subject: Biomedicina description: Doxorubicin (Dox), one of the most used chemotherapeutic agents, is known to generate oxidative stress and block DNA synthesis, which result in severe dose-limiting toxicity. A strategy to protect against Dox toxic effects could be to use dietary antioxidants of which fruits and vegetable are a rich source. In this context, strawberry consumption is associated with the maintenance of good health and the prevention of several diseases, thanks to the antioxidant capacities of its bioactive compounds. The aim of the present study was to evaluate the protective effects of strawberry consumption against oxidative stress induced by Dox in rats. Animals were fed with strawberry enriched diet (15% of the total calories) for two months and Dox (10 mg/kg; i.p.) was injected at the end of the experimental period. Strawberry consumption significantly inhibited ROS production and oxidative damage biomarkers accumulation in plasma and liver tissue and alleviated histopathological changes in rat livers treated with Dox. The reduction of antioxidant enzyme activities was significantly mitigated after strawberry consumption. In addition, strawberry enriched diet ameliorated liver mitochondrial antioxidant levels and functionality. In conclusion, strawberry intake protects against Dox-induced toxicity, at plasma, liver and mitochondrial levels thanks to its high contents of bioactive compounds. date: 2016 type: Artículo type: PeerReviewed identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica Cerrado Inglés Doxorubicin (Dox), one of the most used chemotherapeutic agents, is known to generate oxidative stress and block DNA synthesis, which result in severe dose-limiting toxicity. A strategy to protect against Dox toxic effects could be to use dietary antioxidants of which fruits and vegetable are a rich source. In this context, strawberry consumption is associated with the maintenance of good health and the prevention of several diseases, thanks to the antioxidant capacities of its bioactive compounds. The aim of the present study was to evaluate the protective effects of strawberry consumption against oxidative stress induced by Dox in rats. Animals were fed with strawberry enriched diet (15% of the total calories) for two months and Dox (10 mg/kg; i.p.) was injected at the end of the experimental period. Strawberry consumption significantly inhibited ROS production and oxidative damage biomarkers accumulation in plasma and liver tissue and alleviated histopathological changes in rat livers treated with Dox. The reduction of antioxidant enzyme activities was significantly mitigated after strawberry consumption. In addition, strawberry enriched diet ameliorated liver mitochondrial antioxidant levels and functionality. In conclusion, strawberry intake protects against Dox-induced toxicity, at plasma, liver and mitochondrial levels thanks to its high contents of bioactive compounds. metadata Giampieri, Francesca; Alvarez-Suarez, Jose M.; Gasparrini, Massimiliano; Forbes- Hernandez, Tamara Y.; Afrin, Sadia; Bompadre, Stefano; Rubini, Corrado; Zizzi, Antonio; Astolfi, Paola; Santos-Buelga, Celestino; González-Paramás, Ana M.; Quiles, Josè L.; Mezzetti, Bruno y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, tamara.forbes@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2016) Strawberry consumption alleviates doxorubicin-induced toxicity by suppressing oxidative stress. Food and Chemical Toxicology, 94. pp. 128-137. ISSN 0278-6915 relation: http://doi.org/10.1016/j.fct.2016.06.003 relation: doi:10.1016/j.fct.2016.06.003 language: en relation: http://repositorio.uneatlantico.es/id/eprint/150/ canonical: http://repositorio.uneatlantico.es/id/eprint/150/ title: Targeting Glioblastoma with the Use of Phytocompounds and Nanoparticles creator: Pistollato, Francesca creator: Bremer-Hoffmann, Susanne creator: Basso, Giuseppe creator: Sumalla Cano, Sandra creator: Elío Pascual, Iñaki creator: Masías Vergara, Manuel creator: Giampieri, Francesca creator: Battino, Maurizio subject: Biomedicina description: Glioblastoma multiforme (GBM) are extremely lethal and still poorly treated primary brain tumors, characterized by the presence of highly tumorigenic cancer stem cell (CSC) subpopulations, considered responsible for tumor relapse. In order to successfully eradicate GBM growth and recurrence, new anti-cancer strategies selectively targeting CSCs should be designed. CSCs might be eradicated by targeting some of their cell surface markers and transporters, inducing their differentiation, impacting their hyper-glycolytic metabolism, inhibiting CSC-related signaling pathways and/or by targeting their microenvironmental niche. In this regard, phytocompounds such as curcumin, isothiocyanates, resveratrol and epigallocatechin-3-gallate have been shown to prevent or reverse cancer-related epigenetic dysfunctions, reducing tumorigenesis, preventing metastasis and/or increasing chemotherapy and radiotherapy efficacy. However, the actual bioavailability and metabolic processing of phytocompounds is generally unknown, and the presence of the blood brain barrier often represents a limitation to glioma treatments. Nowadays, nanoparticles (NPs) can be loaded with therapeutic compounds such as phytochemicals, improving their bioavailability and their targeted delivery within the GBM tumor bulk. Moreover, NPs can be designed to increase their tropism and specificity toward CSCs by conjugating their surface with antibodies specific for CSC antigens, with ligands or with glucose analogues. Here we discuss the use of phytochemicals as anti-glioma agents and the applicability of phytochemical-loaded NPs as drug delivery systems to target GBM. Additionally, we provide some examples on how NPs can be specifically formulated to improve CSC targeting. date: 2016 type: Artículo type: PeerReviewed identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica Cerrado Inglés Glioblastoma multiforme (GBM) are extremely lethal and still poorly treated primary brain tumors, characterized by the presence of highly tumorigenic cancer stem cell (CSC) subpopulations, considered responsible for tumor relapse. In order to successfully eradicate GBM growth and recurrence, new anti-cancer strategies selectively targeting CSCs should be designed. CSCs might be eradicated by targeting some of their cell surface markers and transporters, inducing their differentiation, impacting their hyper-glycolytic metabolism, inhibiting CSC-related signaling pathways and/or by targeting their microenvironmental niche. In this regard, phytocompounds such as curcumin, isothiocyanates, resveratrol and epigallocatechin-3-gallate have been shown to prevent or reverse cancer-related epigenetic dysfunctions, reducing tumorigenesis, preventing metastasis and/or increasing chemotherapy and radiotherapy efficacy. However, the actual bioavailability and metabolic processing of phytocompounds is generally unknown, and the presence of the blood brain barrier often represents a limitation to glioma treatments. Nowadays, nanoparticles (NPs) can be loaded with therapeutic compounds such as phytochemicals, improving their bioavailability and their targeted delivery within the GBM tumor bulk. Moreover, NPs can be designed to increase their tropism and specificity toward CSCs by conjugating their surface with antibodies specific for CSC antigens, with ligands or with glucose analogues. Here we discuss the use of phytochemicals as anti-glioma agents and the applicability of phytochemical-loaded NPs as drug delivery systems to target GBM. Additionally, we provide some examples on how NPs can be specifically formulated to improve CSC targeting. metadata Pistollato, Francesca; Bremer-Hoffmann, Susanne; Basso, Giuseppe; Sumalla Cano, Sandra; Elío Pascual, Iñaki; Masías Vergara, Manuel; Giampieri, Francesca y Battino, Maurizio mail francesca.pistollato@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, sandra.sumalla@uneatlantico.es, inaki.elio@uneatlantico.es, manuel.masias@uneatlantico.es, francesca.giampieri@uneatlantico.es, maurizio.battino@uneatlantico.es (2016) Targeting Glioblastoma with the Use of Phytocompounds and Nanoparticles. Targeted Oncology, 11 (1). pp. 1-16. ISSN 1776-2596 relation: http://doi.org/10.1007/s11523-015-0378-5 relation: doi:10.1007/s11523-015-0378-5 language: en relation: http://repositorio.uneatlantico.es/id/eprint/95/ canonical: http://repositorio.uneatlantico.es/id/eprint/95/ title: Associations between Sleep, Cortisol Regulation, and Diet: Possible Implications for the Risk of Alzheimer Disease creator: Pistollato, Francesca creator: Sumalla Cano, Sandra creator: Elío Pascual, Iñaki creator: Masias Vergara, Manuel creator: Giampieri, Francesca creator: Battino, Maurizio subject: Alimentación description: Accumulation of proteinaceous amyloid β plaques and tau oligomers may occur several years before the onset of Alzheimer disease (AD). Under normal circumstances, misfolded proteins get cleared by proteasome degradation, autophagy, and the recently discovered brain glymphatic system, an astroglial-mediated interstitial fluid bulk flow. It has been shown that the activity of the glymphatic system is higher during sleep and disengaged or low during wakefulness. As a consequence, poor sleep quality, which is associated with dementia, might negatively affect glymphatic system activity, thus contributing to amyloid accumulation. The diet is another important factor to consider in the regulation of this complex network. Diets characterized by high intakes of refined sugars, salt, animal-derived proteins and fats and by low intakes of fruit and vegetables are associated with a higher risk of AD and can perturb the circadian modulation of cortisol secretion, which is associated with poor sleep quality. For this reason, diets and nutritional interventions aimed at restoring cortisol concentrations may ease sleep disorders and may facilitate brain clearance, consequentially reducing the risk of cognitive impairment and dementia. Here, we describe the associations that exist between sleep, cortisol regulation, and diet and their possible implications for the risk of cognitive impairment and AD. date: 2016 type: Artículo type: PeerReviewed identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica SIN ESPECIFICAR SIN ESPECIFICAR Accumulation of proteinaceous amyloid β plaques and tau oligomers may occur several years before the onset of Alzheimer disease (AD). Under normal circumstances, misfolded proteins get cleared by proteasome degradation, autophagy, and the recently discovered brain glymphatic system, an astroglial-mediated interstitial fluid bulk flow. It has been shown that the activity of the glymphatic system is higher during sleep and disengaged or low during wakefulness. As a consequence, poor sleep quality, which is associated with dementia, might negatively affect glymphatic system activity, thus contributing to amyloid accumulation. The diet is another important factor to consider in the regulation of this complex network. Diets characterized by high intakes of refined sugars, salt, animal-derived proteins and fats and by low intakes of fruit and vegetables are associated with a higher risk of AD and can perturb the circadian modulation of cortisol secretion, which is associated with poor sleep quality. For this reason, diets and nutritional interventions aimed at restoring cortisol concentrations may ease sleep disorders and may facilitate brain clearance, consequentially reducing the risk of cognitive impairment and dementia. Here, we describe the associations that exist between sleep, cortisol regulation, and diet and their possible implications for the risk of cognitive impairment and AD. metadata Pistollato, Francesca; Sumalla Cano, Sandra; Elío Pascual, Iñaki; Masias Vergara, Manuel; Giampieri, Francesca y Battino, Maurizio mail francesca.pistollato@uneatlantico.es, sandra.sumalla@uneatlantico.es, inaki.elio@uneatlantico.es, manuel.masias@uneatlantico.es, francesca.giampieri@uneatlantico.es, maurizio.battino@uneatlantico.es (2016) Associations between Sleep, Cortisol Regulation, and Diet: Possible Implications for the Risk of Alzheimer Disease. Advances in Nutrition: An International Review Journal, 7 (4). pp. 679-689. ISSN 2156-5376 relation: http://doi.org/10.3945/an.115.011775 relation: doi:10.3945/an.115.011775 relation: http://repositorio.uneatlantico.es/id/eprint/96/ canonical: http://repositorio.uneatlantico.es/id/eprint/96/ title: Role of gut microbiota and nutrients in amyloid formation and pathogenesis of Alzheimer disease creator: Pistollato, Francesca creator: Sumalla Cano, Sandra creator: Elío Pascual, Iñaki creator: Masias Vergara, Manuel creator: Giampieri, Francesca creator: Battino, Maurizio subject: Alimentación description: It has been hypothesized that alterations in the composition of the gut microbiota might be associated with the onset of certain human pathologies, such as Alzheimer disease, a neurodegenerative syndrome associated with cerebral accumulation of amyloid-β fibrils. It has been shown that bacteria populating the gut microbiota can release significant amounts of amyloids and lipopolysaccharides, which might play a role in the modulation of signaling pathways and the production of proinflammatory cytokines related to the pathogenesis of Alzheimer disease. Additionally, nutrients have been shown to affect the composition of the gut microbiota as well as the formation and aggregation of cerebral amyloid-β. This suggests that modulating the gut microbiome and amyloidogenesis through specific nutritional interventions might prove to be an effective strategy to prevent or reduce the risk of Alzheimer disease. This review examines the possible role of the gut in the dissemination of amyloids, the role of the gut microbiota in the regulation of the gut–brain axis, the potential amyloidogenic properties of gut bacteria, and the possible impact of nutrients on modulation of microbiota composition and amyloid formation in relation to the pathogenesis of Alzheimer disease. date: 2016 type: Artículo type: PeerReviewed identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica Universidad Internacional Iberoamericana Puerto Rico > Investigación > Producción Científica Abierto Inglés It has been hypothesized that alterations in the composition of the gut microbiota might be associated with the onset of certain human pathologies, such as Alzheimer disease, a neurodegenerative syndrome associated with cerebral accumulation of amyloid-β fibrils. It has been shown that bacteria populating the gut microbiota can release significant amounts of amyloids and lipopolysaccharides, which might play a role in the modulation of signaling pathways and the production of proinflammatory cytokines related to the pathogenesis of Alzheimer disease. Additionally, nutrients have been shown to affect the composition of the gut microbiota as well as the formation and aggregation of cerebral amyloid-β. This suggests that modulating the gut microbiome and amyloidogenesis through specific nutritional interventions might prove to be an effective strategy to prevent or reduce the risk of Alzheimer disease. This review examines the possible role of the gut in the dissemination of amyloids, the role of the gut microbiota in the regulation of the gut–brain axis, the potential amyloidogenic properties of gut bacteria, and the possible impact of nutrients on modulation of microbiota composition and amyloid formation in relation to the pathogenesis of Alzheimer disease. metadata Pistollato, Francesca; Sumalla Cano, Sandra; Elío Pascual, Iñaki; Masias Vergara, Manuel; Giampieri, Francesca y Battino, Maurizio mail francesca.pistollato@uneatlantico.es, sandra.sumalla@uneatlantico.es, inaki.elio@uneatlantico.es, manuel.masias@uneatlantico.es, francesca.giampieri@uneatlantico.es, maurizio.battino@uneatlantico.es (2016) Role of gut microbiota and nutrients in amyloid formation and pathogenesis of Alzheimer disease. Nutrition Reviews, 74 (10). pp. 624-634. ISSN 0029-6643 relation: http://doi.org/10.1093/nutrit/nuw023 relation: doi:10.1093/nutrit/nuw023 language: en relation: http://repositorio.uneatlantico.es/id/eprint/148/ canonical: http://repositorio.uneatlantico.es/id/eprint/148/ title: Coenzyme Q and Its Role in the Dietary Therapy against Aging creator: Varela-López, Alfonso creator: Giampieri, Francesca creator: Battino, Maurizio creator: Quiles, Jose L. subject: Biomedicina description: Coenzyme Q (CoQ) is a naturally occurring molecule located in the hydrophobic domain of the phospholipid bilayer of all biological membranes. Shortly after being discovered, it was recognized as an essential electron transport chain component in mitochondria where it is particularly abundant. Since then, more additional roles in cell physiology have been reported, including antioxidant, signaling, death prevention, and others. It is known that all cells are able to synthesize functionally sufficient amounts of CoQ under normal physiological conditions. However, CoQ is a molecule found in different dietary sources, which can be taken up and incorporated into biological membranes. It is known that mitochondria have a close relationship with the aging process. Additionally, delaying the aging process through diet has aroused the interest of scientists for many years. These observations have stimulated investigation of the anti-aging potential of CoQ and its possible use in dietary therapies to alleviate the effects of aging. In this context, the present review focus on the current knowledge and evidence the roles of CoQ cells, its relationship with aging, and possible implications of dietary CoQ in relation to aging, lifespan or age-related diseases. date: 2016 type: Artículo type: PeerReviewed format: text language: en rights: cc_by_4 identifier: http://repositorio.uneatlantico.es/id/eprint/148/1/molecules-21-00373.pdf identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros SIN ESPECIFICAR SIN ESPECIFICAR Coenzyme Q (CoQ) is a naturally occurring molecule located in the hydrophobic domain of the phospholipid bilayer of all biological membranes. Shortly after being discovered, it was recognized as an essential electron transport chain component in mitochondria where it is particularly abundant. Since then, more additional roles in cell physiology have been reported, including antioxidant, signaling, death prevention, and others. It is known that all cells are able to synthesize functionally sufficient amounts of CoQ under normal physiological conditions. However, CoQ is a molecule found in different dietary sources, which can be taken up and incorporated into biological membranes. It is known that mitochondria have a close relationship with the aging process. Additionally, delaying the aging process through diet has aroused the interest of scientists for many years. These observations have stimulated investigation of the anti-aging potential of CoQ and its possible use in dietary therapies to alleviate the effects of aging. In this context, the present review focus on the current knowledge and evidence the roles of CoQ cells, its relationship with aging, and possible implications of dietary CoQ in relation to aging, lifespan or age-related diseases. metadata Varela-López, Alfonso; Giampieri, Francesca; Battino, Maurizio y Quiles, Jose L. mail SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es, SIN ESPECIFICAR (2016) Coenzyme Q and Its Role in the Dietary Therapy against Aging. Molecules, 21 (3). p. 373. ISSN 1420-3049 relation: http://doi.org/10.3390/molecules21030373 relation: doi:10.3390/molecules21030373 relation: http://repositorio.uneatlantico.es/id/eprint/97/ canonical: http://repositorio.uneatlantico.es/id/eprint/97/ title: Metformin and caloric restriction induce an AMPK-dependent restoration of mitochondrial dysfunction in fibroblasts from Fibromyalgia patients creator: Alcocer-Gómez, Elísabet creator: Garrido-Maraver, Juan creator: Bullón, Pedro creator: Marín-Aguilar, Fabiola creator: Cotán, David creator: Carrión, Angel M. creator: Alvarez-Suarez, José M. creator: Giampieri, Francesca creator: Sánchez-Alcazar, José Antonio creator: Battino, Maurizio creator: Cordero, Mario D. subject: Alimentación description: Impaired AMPK is associated with a wide spectrum of clinical and pathological conditions, ranging from obesity, altered responses to exercise or metabolic syndrome, to inflammation, disturbed mitochondrial biogenesis and defective response to energy stress. Fibromyalgia (FM) is a world-wide diffused musculoskeletal chronic pain condition that affects up to 5% of the general population and comprises all the above mentioned pathophysiological states. Here, we tested the involvement of AMPK activation in fibroblasts derived from FM patients. AMPK was not phosphorylated in fibroblasts from FM patients and was associated with decreased mitochondrial biogenesis, reduced oxygen consumption, decreased antioxidant enzymes expression levels and mitochondrial dysfunction. However, mtDNA sequencing analysis did not show any important alterations which could justify the mitochondrial defects. AMPK activation in FM fibroblast was impaired in response to moderate oxidative stress. In contrast, AMPK activation by metformin or incubation with serum from caloric restricted mice improved the response to moderate oxidative stress and mitochondrial metabolism in FM fibroblasts. These results suggest that AMPK plays an essential role in FM pathophysiology and could represent the basis for a valuable new therapeutic target/strategy. Furthermore, both metformin and caloric restriction could be an interesting therapeutic approach in FM. date: 2015 type: Artículo type: PeerReviewed identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros SIN ESPECIFICAR SIN ESPECIFICAR Impaired AMPK is associated with a wide spectrum of clinical and pathological conditions, ranging from obesity, altered responses to exercise or metabolic syndrome, to inflammation, disturbed mitochondrial biogenesis and defective response to energy stress. Fibromyalgia (FM) is a world-wide diffused musculoskeletal chronic pain condition that affects up to 5% of the general population and comprises all the above mentioned pathophysiological states. Here, we tested the involvement of AMPK activation in fibroblasts derived from FM patients. AMPK was not phosphorylated in fibroblasts from FM patients and was associated with decreased mitochondrial biogenesis, reduced oxygen consumption, decreased antioxidant enzymes expression levels and mitochondrial dysfunction. However, mtDNA sequencing analysis did not show any important alterations which could justify the mitochondrial defects. AMPK activation in FM fibroblast was impaired in response to moderate oxidative stress. In contrast, AMPK activation by metformin or incubation with serum from caloric restricted mice improved the response to moderate oxidative stress and mitochondrial metabolism in FM fibroblasts. These results suggest that AMPK plays an essential role in FM pathophysiology and could represent the basis for a valuable new therapeutic target/strategy. Furthermore, both metformin and caloric restriction could be an interesting therapeutic approach in FM. metadata Alcocer-Gómez, Elísabet; Garrido-Maraver, Juan; Bullón, Pedro; Marín-Aguilar, Fabiola; Cotán, David; Carrión, Angel M.; Alvarez-Suarez, José M.; Giampieri, Francesca; Sánchez-Alcazar, José Antonio; Battino, Maurizio y Cordero, Mario D. mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, jose.alvarez@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es, SIN ESPECIFICAR (2015) Metformin and caloric restriction induce an AMPK-dependent restoration of mitochondrial dysfunction in fibroblasts from Fibromyalgia patients. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1852 (7). pp. 1257-1267. ISSN 0925-4439 relation: http://doi.org/10.1016/j.bbadis.2015.03.005 relation: doi:10.1016/j.bbadis.2015.03.005 relation: http://repositorio.uneatlantico.es/id/eprint/98/ canonical: http://repositorio.uneatlantico.es/id/eprint/98/ title: Lipophilic antioxidants prevent lipopolysaccharide-induced mitochondrial dysfunction through mitochondrial biogenesis improvement creator: Bullón, Pedro creator: Román-Malo, Lourdes creator: Marín-Aguilar, Fabiola creator: Alvarez-Suarez, José Miguel creator: Giampieri, Francesca creator: Battino, Maurizio creator: Cordero, Mario D. subject: Alimentación description: Oxidative stress is implicated in several infectious diseases. In this regard, lipopolysaccharide (LPS), an endotoxic component, induces mitochondrial dysfunction and oxidative stress in several pathological events such as periodontal disease or sepsis. In our experiments, LPS-treated fibroblasts provoked increased oxidative stress, mitochondrial dysfunction, reduced oxygen consumption and mitochondrial biogenesis. After comparing coenzyme Q10 (CoQ10) and N-acetylcysteine (NAC), we observed a more significant protection of CoQ10 than of NAC, which was comparable with other lipophilic and hydrophilic antioxidants such as vitamin E or BHA respectively. CoQ10 improved mitochondrial biogenesis by activating PGC-1α and TFAM. This lipophilic antioxidant protection was observed in mice after LPS injection. These results show that mitochondria-targeted lipophilic antioxidants could be a possible specific therapeutic strategy in pharmacology in the treatment of infectious diseases and their complications. date: 2015 type: Artículo type: PeerReviewed identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica SIN ESPECIFICAR SIN ESPECIFICAR Oxidative stress is implicated in several infectious diseases. In this regard, lipopolysaccharide (LPS), an endotoxic component, induces mitochondrial dysfunction and oxidative stress in several pathological events such as periodontal disease or sepsis. In our experiments, LPS-treated fibroblasts provoked increased oxidative stress, mitochondrial dysfunction, reduced oxygen consumption and mitochondrial biogenesis. After comparing coenzyme Q10 (CoQ10) and N-acetylcysteine (NAC), we observed a more significant protection of CoQ10 than of NAC, which was comparable with other lipophilic and hydrophilic antioxidants such as vitamin E or BHA respectively. CoQ10 improved mitochondrial biogenesis by activating PGC-1α and TFAM. This lipophilic antioxidant protection was observed in mice after LPS injection. These results show that mitochondria-targeted lipophilic antioxidants could be a possible specific therapeutic strategy in pharmacology in the treatment of infectious diseases and their complications. metadata Bullón, Pedro; Román-Malo, Lourdes; Marín-Aguilar, Fabiola; Alvarez-Suarez, José Miguel; Giampieri, Francesca; Battino, Maurizio y Cordero, Mario D. mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, josemanuel.alvarez@unini.edu.mx, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es, SIN ESPECIFICAR (2015) Lipophilic antioxidants prevent lipopolysaccharide-induced mitochondrial dysfunction through mitochondrial biogenesis improvement. Pharmacological Research, 91. pp. 1-8. ISSN 10436618 relation: http://doi.org/10.1016/j.phrs.2014.10.007 relation: doi:10.1016/j.phrs.2014.10.007 relation: http://repositorio.uneatlantico.es/id/eprint/149/ canonical: http://repositorio.uneatlantico.es/id/eprint/149/ title: Strawberry as a health promoter: an evidence based review creator: Giampieri, Francesca creator: Forbes-Hernandez, Tamara Y. creator: Gasparrini, Massimiliano creator: Alvarez-Suarez, José M. creator: Afrin, Sadia creator: Bompadre, Stefano creator: Quiles, José L. creator: Mezzetti, Bruno creator: Battino, Maurizio subject: Biomedicina description: Since a high intake of fruits and vegetables is inversely related to the incidence of several degenerative diseases, the importance of a balanced diet in relation to human health has received increased consumer attention worldwide. Strawberries (Fragaria X ananassa, Duch.) are a rich source of a wide variety of nutritive compounds such as sugars, vitamins, and minerals, as well as non-nutritive, bioactive compounds such as flavonoids, anthocyanins and phenolic acids. All of these compounds exert a synergistic and cumulative effect on human health promotion and in disease prevention. Strawberry phenolics are indeed able (i) to detoxify free radicals blocking their production, (ii) to modulate the expression of genes involved in metabolism, cell survival and proliferation and antioxidant defense, and (iii) to protect and repair DNA damage. The overall objective of the present review is to update and discuss the key findings, from recent in vivo studies, on the effects of strawberries on human health. Particular attention will be paid to the molecular mechanisms proposed to explain the health effects of polyphenols against the most common diseases related to oxidative stress driven pathologies, such as cancer, cardiovascular diseases, type II diabetes, obesity and neurodegenerative diseases, and inflammation. date: 2015 type: Artículo type: PeerReviewed identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Cerrado Inglés Since a high intake of fruits and vegetables is inversely related to the incidence of several degenerative diseases, the importance of a balanced diet in relation to human health has received increased consumer attention worldwide. Strawberries (Fragaria X ananassa, Duch.) are a rich source of a wide variety of nutritive compounds such as sugars, vitamins, and minerals, as well as non-nutritive, bioactive compounds such as flavonoids, anthocyanins and phenolic acids. All of these compounds exert a synergistic and cumulative effect on human health promotion and in disease prevention. Strawberry phenolics are indeed able (i) to detoxify free radicals blocking their production, (ii) to modulate the expression of genes involved in metabolism, cell survival and proliferation and antioxidant defense, and (iii) to protect and repair DNA damage. The overall objective of the present review is to update and discuss the key findings, from recent in vivo studies, on the effects of strawberries on human health. Particular attention will be paid to the molecular mechanisms proposed to explain the health effects of polyphenols against the most common diseases related to oxidative stress driven pathologies, such as cancer, cardiovascular diseases, type II diabetes, obesity and neurodegenerative diseases, and inflammation. metadata Giampieri, Francesca; Forbes-Hernandez, Tamara Y.; Gasparrini, Massimiliano; Alvarez-Suarez, José M.; Afrin, Sadia; Bompadre, Stefano; Quiles, José L.; Mezzetti, Bruno y Battino, Maurizio mail SIN ESPECIFICAR, tamara.forbes@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2015) Strawberry as a health promoter: an evidence based review. Food & Function, 6 (5). pp. 1386-1398. ISSN 2042-6496 relation: http://doi.org/10.1039/c5fo00147a relation: doi:10.1039/c5fo00147a language: en relation: http://repositorio.uneatlantico.es/id/eprint/94/ canonical: http://repositorio.uneatlantico.es/id/eprint/94/ title: The genetic aspects of berries: from field to health creator: Mazzoni, Luca creator: Perez-Lopez, Patricia creator: Giampieri, Francesca creator: Alvarez-Suarez, José M. creator: Gasparrini, Massimiliano creator: Forbes-Hernandez, Tamara Y. creator: Quiles, José L. creator: Mezzetti, Bruno creator: Battino, Maurizio subject: Alimentación description: Berries are a relevant source of micronutrients and nonessential phytochemicals, such as polyphenol compounds, that play a synergistic and cumulative role in human health promotion. Several systematic analyses showed that berry phenolics are able to detoxify reactive oxygen and nitrogen species, blocking their production, to intervene in the cell cycle, participating in the transduction and expression of genes involved in apoptosis, and to repair oxidative DNA damage. As a consequence, the improvement of the nutritional quality of berries has become a new quality target of breeding and biotechnological strategies, to control or to increase the content of specific health-related compounds in fruits. This work reviews, on the basis of the in vitro and in vivo evidence, the main berries' phytochemical compounds and their possible mechanisms of action on pathways involved in several type of diseases, with particular attention to cancer, inflammation, neurodegeneration, diabetes and cardiovascular diseases. © 2015 Society of Chemical Industry date: 2015 type: Artículo type: PeerReviewed identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica SIN ESPECIFICAR SIN ESPECIFICAR Berries are a relevant source of micronutrients and nonessential phytochemicals, such as polyphenol compounds, that play a synergistic and cumulative role in human health promotion. Several systematic analyses showed that berry phenolics are able to detoxify reactive oxygen and nitrogen species, blocking their production, to intervene in the cell cycle, participating in the transduction and expression of genes involved in apoptosis, and to repair oxidative DNA damage. As a consequence, the improvement of the nutritional quality of berries has become a new quality target of breeding and biotechnological strategies, to control or to increase the content of specific health-related compounds in fruits. This work reviews, on the basis of the in vitro and in vivo evidence, the main berries' phytochemical compounds and their possible mechanisms of action on pathways involved in several type of diseases, with particular attention to cancer, inflammation, neurodegeneration, diabetes and cardiovascular diseases. © 2015 Society of Chemical Industry metadata Mazzoni, Luca; Perez-Lopez, Patricia; Giampieri, Francesca; Alvarez-Suarez, José M.; Gasparrini, Massimiliano; Forbes-Hernandez, Tamara Y.; Quiles, José L.; Mezzetti, Bruno y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, tamara.forbes@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2015) The genetic aspects of berries: from field to health. Journal of the Science of Food and Agriculture, 96 (2). pp. 365-371. ISSN 00225142 relation: http://doi.org/10.1002/jsfa.7216 relation: doi:10.1002/jsfa.7216 relation: http://repositorio.uneatlantico.es/id/eprint/100/ canonical: http://repositorio.uneatlantico.es/id/eprint/100/ title: The use of plant-derived bioactive compounds to target cancer stem cells and modulate tumor microenvironment creator: Pistollato, Francesca creator: Giampieri, Francesca creator: Battino, Maurizio subject: Alimentación description: In the last decades cancer has been considered as an epigenetic dysfunction, given the profound role of diet and lifestyle in cancer prevention and the determination of cancer risk. A plethora of recent publications have addressed the specific role of several environmental factors, such as nutritional habits, behavior, stress and toxins in the regulation of the physiological and cancer epigenome. In particular, plant-derived bioactive nutrients have been seen to positively affect normal cell growth, proliferation and differentiation and also to revert cancer related epigenetic dysfunctions, reducing tumorigenesis, preventing metastasis and/or increasing chemo and radiotherapy efficacy. Moreover, virtually all cancer types are characterized by the presence of cancer stem cell (CSC) subpopulations, residing in specific hypoxic and acidic microenvironments, or niches, and these cells are currently considered responsible for tumor resistance to therapy and tumor relapse. Modern anti-cancer strategies should be designed to selectively target CSCs and modulate the hypoxic and acidic tumor microenvironment, and, to this end, natural bioactive components seem to play a role. This review aims to discuss the effects elicited by plant-derived bioactive nutrients in the regulation of CSC self-renewal, cancer metabolism and tumor microenvironment. date: 2015 type: Artículo type: PeerReviewed identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica Cerrado Inglés In the last decades cancer has been considered as an epigenetic dysfunction, given the profound role of diet and lifestyle in cancer prevention and the determination of cancer risk. A plethora of recent publications have addressed the specific role of several environmental factors, such as nutritional habits, behavior, stress and toxins in the regulation of the physiological and cancer epigenome. In particular, plant-derived bioactive nutrients have been seen to positively affect normal cell growth, proliferation and differentiation and also to revert cancer related epigenetic dysfunctions, reducing tumorigenesis, preventing metastasis and/or increasing chemo and radiotherapy efficacy. Moreover, virtually all cancer types are characterized by the presence of cancer stem cell (CSC) subpopulations, residing in specific hypoxic and acidic microenvironments, or niches, and these cells are currently considered responsible for tumor resistance to therapy and tumor relapse. Modern anti-cancer strategies should be designed to selectively target CSCs and modulate the hypoxic and acidic tumor microenvironment, and, to this end, natural bioactive components seem to play a role. This review aims to discuss the effects elicited by plant-derived bioactive nutrients in the regulation of CSC self-renewal, cancer metabolism and tumor microenvironment. metadata Pistollato, Francesca; Giampieri, Francesca y Battino, Maurizio mail francesca.pistollato@uneatlantico.es, francesca.giampieri@uneatlantico.es, maurizio.battino@uneatlantico.es (2015) The use of plant-derived bioactive compounds to target cancer stem cells and modulate tumor microenvironment. Food and Chemical Toxicology, 75. pp. 58-70. ISSN 02786915 relation: http://doi.org/10.1016/j.fct.2014.11.004 relation: doi:10.1016/j.fct.2014.11.004 language: en relation: http://repositorio.uneatlantico.es/id/eprint/101/ canonical: http://repositorio.uneatlantico.es/id/eprint/101/ title: Plant-Based and Plant-Rich Diet Patterns during Gestation: Beneficial Effects and Possible Shortcomings creator: Pistollato, Francesca creator: Sumalla Cano, Sandra creator: Elio Pascual, Iñaki creator: Masias Vergara, Manuel creator: Giampieri, Francesca creator: Battino, Maurizio subject: Alimentación description: Environmental and lifestyle factors are known to play an important role during gestation, determining newborns' health status and influencing their risk of being subject to certain noncommunicable diseases later in life. In particular, maternal nutritional patterns characterized by a low intake of plant-derived foods could increase the risk of gestation-related issues, such as preeclampsia and pregravid obesity, increase genotoxicant susceptibility, and contribute to the onset of pediatric diseases. In particular, the risk of pediatric wheeze, diabetes, neural tube defects, orofacial clefts, and some pediatric tumors seems to be reduced by maternal intake of adequate amounts of vegetables, fruits, and selected antioxidants. Nevertheless, plant-based diets, like any other diet, if improperly balanced, could be deficient in some specific nutrients that are particularly relevant during gestation, such as n–3 (ω-3) fatty acids, vitamin B-12, iron, zinc, and iodine, possibly affecting the offspring's health state. Here we review the scientific literature in this field, focusing specifically on observational studies in humans, and highlight protective effects elicited by maternal diets enriched in plant-derived foods and possible issues related to maternal plant-based diets. date: 2015 type: Artículo type: PeerReviewed identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica SIN ESPECIFICAR SIN ESPECIFICAR Environmental and lifestyle factors are known to play an important role during gestation, determining newborns' health status and influencing their risk of being subject to certain noncommunicable diseases later in life. In particular, maternal nutritional patterns characterized by a low intake of plant-derived foods could increase the risk of gestation-related issues, such as preeclampsia and pregravid obesity, increase genotoxicant susceptibility, and contribute to the onset of pediatric diseases. In particular, the risk of pediatric wheeze, diabetes, neural tube defects, orofacial clefts, and some pediatric tumors seems to be reduced by maternal intake of adequate amounts of vegetables, fruits, and selected antioxidants. Nevertheless, plant-based diets, like any other diet, if improperly balanced, could be deficient in some specific nutrients that are particularly relevant during gestation, such as n–3 (ω-3) fatty acids, vitamin B-12, iron, zinc, and iodine, possibly affecting the offspring's health state. Here we review the scientific literature in this field, focusing specifically on observational studies in humans, and highlight protective effects elicited by maternal diets enriched in plant-derived foods and possible issues related to maternal plant-based diets. metadata Pistollato, Francesca; Sumalla Cano, Sandra; Elio Pascual, Iñaki; Masias Vergara, Manuel; Giampieri, Francesca y Battino, Maurizio mail francesca.pistollato@uneatlantico.es, sandra.sumalla@uneatlantico.es, inaki.elio@uneatlantico.es, manuel.masias@uneatlantico.es, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2015) Plant-Based and Plant-Rich Diet Patterns during Gestation: Beneficial Effects and Possible Shortcomings. Advances in Nutrition, 6 (5). pp. 581-591. ISSN 2161-8313 relation: http://doi.org/10.3945/an.115.009126 relation: doi:10.3945/an.115.009126 relation: http://repositorio.uneatlantico.es/id/eprint/103/ canonical: http://repositorio.uneatlantico.es/id/eprint/103/ title: PP194-MON assessing the nutritional intake and the profile of a latin american group of nutrition postgraduate students creator: Elío Pascual, Iñaki creator: Sumalla Cano, Sandra creator: Dominguez Azpíroz, Irma creator: Jarrin, Sandra creator: Montejo, Marylin creator: Battino, Maurizio subject: Alimentación date: 2013 type: Artículo type: PeerReviewed identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros SIN ESPECIFICAR SIN ESPECIFICAR SIN ESPECIFICAR metadata Elío Pascual, Iñaki; Sumalla Cano, Sandra; Dominguez Azpíroz, Irma; Jarrin, Sandra; Montejo, Marylin y Battino, Maurizio mail inaki.elio@uneatlantico.es, sandra.sumalla@uneatlantico.es, irma.dominguez@uneatlantico.es, sandra.jarrin@uneatlantico.es, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2013) PP194-MON assessing the nutritional intake and the profile of a latin american group of nutrition postgraduate students. Clinical Nutrition, 32. S194. ISSN 0261-5614 relation: http://doi.org/10.1016/S0261-5614(13)60504-X relation: doi:10.1016/S0261-5614(13)60504-X