Sequential Changes of NLRP3 Inflammasome Activation in Sepsis and its Relationship With Death
Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Abierto Inglés Introduction: Inflammasomes are recognized as key components of the innate immune response in sepsis. We aimed to describe the transcriptional expression of nucleotide-binding domain, leucine-rich repeat–containing receptor, pyrin domain–containing-3 (NLRP3), and serum interleukin-1β (IL-1 β) in critically ill patients, their changes over the first week and their prognostic value in septic patients. Methods: Prospective study including patients with sepsis based on Sepsis-3 definitions and a control group of critically ill patients without sepsis. We measured the circulating levels of IL-1β as well as the transcriptional expression of NLRP3 at admission and on days 3 and 7. Caspase-1 and caspase-3 activation was analyzed in a matched cohort of patients with septic shock (four dead and four survivors). Results: Fifty-five septic patients and 11 non-septic patients were studied. Levels on day 0 and 3 of IL-1 β and NLRP3 inflammasome expression were significantly higher in patients with sepsis than in controls. NLRP3 was significantly higher in septic patients who survived at day 7 without significant difference between survivors and non-survivors at baseline and on day 3. In survivors, an increased caspase-1 protein expression with reduced expression caspase-3 was observed with the opposite pattern in those who died. Conclusions: NLRP3 is activated in critically ill patients but this up-regulation is more intense in patients with sepsis. In sepsis, a sustained NLRP3 activation during the first week is protective and sepsis. An increased caspase-1 protein expression with reduced expression caspase-3 is the pattern observed in septic shock patients who survive. metadata Garnacho-Montero, José; Palacios-García, Inmaculada; Díaz-Martín, Ana; Gutiérrez-Pizarraya, Antonio; López-Sánchez, José M.; Gómez, Elísabet Alcocer y Cordero, Mario D. mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, mario.cordero@uneatlantico.es (2020) Sequential Changes of NLRP3 Inflammasome Activation in Sepsis and its Relationship With Death. Shock, 54 (3). pp. 294-300. ISSN 1073-2322
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Introduction: Inflammasomes are recognized as key components of the innate immune response in sepsis. We aimed to describe the transcriptional expression of nucleotide-binding domain, leucine-rich repeat–containing receptor, pyrin domain–containing-3 (NLRP3), and serum interleukin-1β (IL-1 β) in critically ill patients, their changes over the first week and their prognostic value in septic patients. Methods: Prospective study including patients with sepsis based on Sepsis-3 definitions and a control group of critically ill patients without sepsis. We measured the circulating levels of IL-1β as well as the transcriptional expression of NLRP3 at admission and on days 3 and 7. Caspase-1 and caspase-3 activation was analyzed in a matched cohort of patients with septic shock (four dead and four survivors). Results: Fifty-five septic patients and 11 non-septic patients were studied. Levels on day 0 and 3 of IL-1 β and NLRP3 inflammasome expression were significantly higher in patients with sepsis than in controls. NLRP3 was significantly higher in septic patients who survived at day 7 without significant difference between survivors and non-survivors at baseline and on day 3. In survivors, an increased caspase-1 protein expression with reduced expression caspase-3 was observed with the opposite pattern in those who died. Conclusions: NLRP3 is activated in critically ill patients but this up-regulation is more intense in patients with sepsis. In sepsis, a sustained NLRP3 activation during the first week is protective and sepsis. An increased caspase-1 protein expression with reduced expression caspase-3 is the pattern observed in septic shock patients who survive.
| Tipo de Documento: | Artículo |
|---|---|
| Palabras Clave: | Caspase-1; Caspase-3; inflammation ; Mortality; NLRP3 inflammasome NLRP3 inflammasome; Sepsis. |
| Clasificación temática: | Materias > Biomedicina |
| Divisiones: | Universidad Europea del Atlántico > Investigación > Artículos y libros |
| Depositante: | Usuarios 0 no encontrado. |
| Depositado: | 01 Jun 2021 23:55 |
| Ultima Modificación: | 03 Jul 2023 23:30 |
| URI: | https://repositorio.uneatlantico.es/id/eprint/119 |
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- Sequential Changes of NLRP3 Inflammasome Activation in Sepsis and its Relationship With Death. (deposited 01 Jun 2021 23:55) [Mostrada Ahora]
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- Garnacho-Montero, José; Palacios-García, Inmaculada; Díaz-Martín, Ana; Gutiérrez-Pizarraya, Antonio; López-Sánchez, José M.; Gómez, Elísabet Alcocer y Cordero, Mario D. Sequential Changes of NLRP3 Inflammasome Activation in Sepsis and its Relationship With Death. (deposited 01 Jun 2021 23:55) [Mostrada Ahora]
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