eprintid: 90 rev_number: 7 eprint_status: archive userid: 2 importid: 0 dir: disk0/00/00/00/90 datestamp: 2021-05-31 14:17:22 lastmod: 2021-06-30 15:38:29 status_changed: 2021-05-31 14:17:22 type: article succeeds: 0 commentary: 0 metadata_visibility: show item_issues_count: 0 sword_depositor: 0 creators_name: Teng, Hui creators_name: Lin, Qiyang creators_name: Li, Kang creators_name: Yuan, Benyao creators_name: Song, Hongbo creators_name: Peng, Hongquan creators_name: Yi, Lunzhao creators_name: Wei, Ming-Chi creators_name: Yang, Yu-Chiao creators_name: Battino, Maurizio creators_name: Cespedes Acuña, Carlos L. creators_name: Chen, Lei creators_name: Xiao, Jianbo creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: maurizio.battino@uneatlantico.es creators_id: creators_id: creators_id: title: Hepatoprotective effects of raspberry (Rubus coreanus Miq.) seed oil and its major constituents ispublished: pub subjects: uneat_sn divisions: uneatlantico_produccion_cientifica full_text_status: none keywords: Raspberry seeds; Fatty acids; Extraction; Antioxidant enzyme; Hepatoprotection abstract: Raspberry seed is a massive byproduct of raspberry juice and wine but usually discarded. The present study employed a microwave-assisted method for extraction of raspberry seed oil (RSO). The results revealed that omega-6 fatty acids (linoleic acid and γ-linolenic acid) were the major constituents in RSO. Cellular antioxidant enzyme activity such as superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were investigated in HepG2 cells treated with RSO. Induction of the synthesis of several antioxidants in H2O2-exposed HepG2 cells was found. RSO increased the enzyme activity of SOD, CAT, and GPx in H2O2-exposed HepG2. Furthermore, RSO inhibited the phosphorylation of upstream mitogen-activated protein kinases (MAPK) such as c-Jun N-terminal kinase (c-JNK) and extracellular signal-regulated kinase (ERK). Taken together, the possible mechanisms to increase antioxidant enzyme activities in HepG2 may through the suppression of ERK and JNK phosphorylation. Raspberry seed oil exhibited good effects on the activities of the intracellular antioxidant enzymes and seems to protect the liver from oxidative stress through the inhibition of MAPKs. date: 2017-00-00 date_type: published publication: Food and Chemical Toxicology volume: 110 pagerange: 418-424 pages: 0 id_number: doi:10.1016/j.fct.2017.09.010 refereed: TRUE issn: 0278-6915 official_url: http://doi.org/10.1016/j.fct.2017.09.010 num_pieces: 0 gscholar_impact: 0 gscholar_datestamp: 0000-00-00 00:00:00 citation: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica SIN ESPECIFICAR SIN ESPECIFICAR Raspberry seed is a massive byproduct of raspberry juice and wine but usually discarded. The present study employed a microwave-assisted method for extraction of raspberry seed oil (RSO). The results revealed that omega-6 fatty acids (linoleic acid and γ-linolenic acid) were the major constituents in RSO. Cellular antioxidant enzyme activity such as superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were investigated in HepG2 cells treated with RSO. Induction of the synthesis of several antioxidants in H2O2-exposed HepG2 cells was found. RSO increased the enzyme activity of SOD, CAT, and GPx in H2O2-exposed HepG2. Furthermore, RSO inhibited the phosphorylation of upstream mitogen-activated protein kinases (MAPK) such as c-Jun N-terminal kinase (c-JNK) and extracellular signal-regulated kinase (ERK). Taken together, the possible mechanisms to increase antioxidant enzyme activities in HepG2 may through the suppression of ERK and JNK phosphorylation. Raspberry seed oil exhibited good effects on the activities of the intracellular antioxidant enzymes and seems to protect the liver from oxidative stress through the inhibition of MAPKs. metadata Teng, Hui; Lin, Qiyang; Li, Kang; Yuan, Benyao; Song, Hongbo; Peng, Hongquan; Yi, Lunzhao; Wei, Ming-Chi; Yang, Yu-Chiao; Battino, Maurizio; Cespedes Acuña, Carlos L.; Chen, Lei y Xiao, Jianbo mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2017) Hepatoprotective effects of raspberry (Rubus coreanus Miq.) seed oil and its major constituents. Food and Chemical Toxicology, 110. pp. 418-424. ISSN 0278-6915