TY  - JOUR
TI  - Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells
ID  - uneatlantico87
N2  - Food fortification through the increase and/or modulation of bioactive compounds has become a major goal for preventing several diseases, including cancer. Here, strawberry lines of cv. Calypso transformed with a construct containing an anthocyanidin synthase (ANS) gene were produced to study the effects on anthocyanin biosynthesis, metabolism, and transcriptome. Three strawberry ANS transgenic lines (ANS L5, ANS L15, and ANS L18) were analyzed for phytochemical composition and total antioxidant capacity (TAC), and their fruit extracts were assessed for cytotoxic effects on hepatocellular carcinoma. ANS L18 fruits had the highest levels of total phenolics and flavonoids, while those of ANS L15 had the highest anthocyanin concentration; TAC positively correlated with total polyphenol content. Fruit transcriptome was also specifically affected in the polyphenol biosynthesis and in other related metabolic pathways. Fruit extracts of all lines exerted cytotoxic effects in a dose/time-dependent manner, increasing cellular apoptosis and free radical levels and impairing mitochondrial functionality.
UR  - http://doi.org/10.1021/acs.jafc.7b04177
Y1  - 2018///
VL  - 66
AV  - none
KW  - Anthocyanidin synthase; Anticancer effects; Apoptosis; Flavonoids; RNA-seq.
A1  - Giampieri, Francesca
A1  - Gasparrini, Massimiliano
A1  - Forbes-Hernandez, Tamara Y.
A1  - Mazzoni, Luca
A1  - Capocasa, Franco
A1  - Sabbadini, Silvia
A1  - Alvarez-Suarez, José M.
A1  - Afrin, Sadia
A1  - Rosati, Carlo
A1  - Pandolfini, Tiziana
A1  - Molesini, Barbara
A1  - Sánchez-Sevilla, José Federico
A1  - Amaya, Iraida
A1  - Mezzetti, Bruno
A1  - Battino, Maurizio
JF  - Journal of Agricultural and Food Chemistry
IS  - 3
EP  - 592
SN  - 0021-8561
SP  - 581
ER  -