eprintid: 8683 rev_number: 8 eprint_status: archive userid: 2 dir: disk0/00/00/86/83 datestamp: 2023-09-06 23:30:25 lastmod: 2024-05-07 20:07:51 status_changed: 2023-09-06 23:30:25 type: article metadata_visibility: show creators_name: Sharif, Nadim creators_name: Ahmed, Shamsun Nahar creators_name: Khandaker, Shamim creators_name: Monifa, Nuzhat Haque creators_name: Abusharha, Ali creators_name: Ramírez-Vargas, Debora L. creators_name: Díez, Isabel De la Torre creators_name: Kuc Castilla, Ángel Gabriel creators_name: Talukder, Ali Azam creators_name: Parvez, Anowar Khasru creators_name: Dey, Shuvra Kanti creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: debora.ramirez@unini.edu.mx creators_id: creators_id: creators_id: creators_id: creators_id: title: Multidrug resistance pattern and molecular epidemiology of pathogens among children with diarrhea in Bangladesh, 2019–2021 ispublished: pub subjects: uneat_bm divisions: uneatlantico_produccion_cientifica divisions: uninimx_produccion_cientifica full_text_status: none keywords: Antimicrobial resistance; Bacteria; Diseases; Infectious-disease diagnostics abstract: Antimicrobial and multidrug resistance (MDR) pathogens are becoming one of the major health threats among children. Integrated studies on the molecular epidemiology and prevalence of AMR and MDR diarrheal pathogens are lacking. A total of 404 fecal specimens were collected from children with diarrhea in Bangladesh from January 2019 to December 2021. We used conventional bacteriologic and molecular sequence analysis methods. Phenotypic and genotypic resistance were determined by disk diffusion and molecular sequencing methods. Fisher’s exact tests with 95% confidence intervals (CIs) was performed. Prevalence of bacterial infection was 63% (251 of 404) among children with diarrhea. E. coli (29%) was the most prevalent. E. coli, Shigella spp., V. cholerae, and Salmonella spp., showed the highest frequency of resistance against ceftriaxone (75–85%), and erythromycin (70–75%%). About 10–20% isolates of E. coli, V. cholerae and Shigella spp. showed MDR against cephem, macrolides, and quinolones. Significant association (p value < 0.05) was found between the phenotypic and genotypic resistance. The risk of diarrhea was the highest among the patients co-infected with E. coli and rotavirus [OR 3.6 (95% CI 1.1–5.4) (p = 0.001)] followed by Shigella spp. and rotavirus [OR 3.5 (95% CI 0.5–5.3) (p = 0.001)]. This study will provide an integrated insight of molecular epidemiology and antimicrobial resistance profiling of bacterial pathogens among children with diarrhea in Bangladesh. date: 2023-08 publication: Scientific Reports volume: 13 number: 1 id_number: doi:10.1038/s41598-023-41174-6 refereed: TRUE issn: 2045-2322 official_url: http://doi.org/10.1038/s41598-023-41174-6 access: open language: en citation: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Producción Científica Universidad Internacional Iberoamericana México > Investigación > Producción Científica Abierto Inglés Antimicrobial and multidrug resistance (MDR) pathogens are becoming one of the major health threats among children. Integrated studies on the molecular epidemiology and prevalence of AMR and MDR diarrheal pathogens are lacking. A total of 404 fecal specimens were collected from children with diarrhea in Bangladesh from January 2019 to December 2021. We used conventional bacteriologic and molecular sequence analysis methods. Phenotypic and genotypic resistance were determined by disk diffusion and molecular sequencing methods. Fisher’s exact tests with 95% confidence intervals (CIs) was performed. Prevalence of bacterial infection was 63% (251 of 404) among children with diarrhea. E. coli (29%) was the most prevalent. E. coli, Shigella spp., V. cholerae, and Salmonella spp., showed the highest frequency of resistance against ceftriaxone (75–85%), and erythromycin (70–75%%). About 10–20% isolates of E. coli, V. cholerae and Shigella spp. showed MDR against cephem, macrolides, and quinolones. Significant association (p value < 0.05) was found between the phenotypic and genotypic resistance. The risk of diarrhea was the highest among the patients co-infected with E. coli and rotavirus [OR 3.6 (95% CI 1.1–5.4) (p = 0.001)] followed by Shigella spp. and rotavirus [OR 3.5 (95% CI 0.5–5.3) (p = 0.001)]. This study will provide an integrated insight of molecular epidemiology and antimicrobial resistance profiling of bacterial pathogens among children with diarrhea in Bangladesh. metadata Sharif, Nadim; Ahmed, Shamsun Nahar; Khandaker, Shamim; Monifa, Nuzhat Haque; Abusharha, Ali; Ramírez-Vargas, Debora L.; Díez, Isabel De la Torre; Kuc Castilla, Ángel Gabriel; Talukder, Ali Azam; Parvez, Anowar Khasru y Dey, Shuvra Kanti mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, debora.ramirez@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2023) Multidrug resistance pattern and molecular epidemiology of pathogens among children with diarrhea in Bangladesh, 2019–2021. Scientific Reports, 13 (1). ISSN 2045-2322