eprintid: 83 rev_number: 7 eprint_status: archive userid: 2 importid: 0 dir: disk0/00/00/00/83 datestamp: 2021-05-31 14:17:20 lastmod: 2021-06-30 15:38:28 status_changed: 2021-05-31 14:17:20 type: article succeeds: 0 commentary: 0 metadata_visibility: show item_issues_count: 0 sword_depositor: 0 creators_name: Alvarez-Suarez, José M. creators_name: Giampieri, Francesca creators_name: Gasparrini, Massimiliano creators_name: Mazzoni, Luca creators_name: Forbes-Hernández, Tamara Y. creators_name: Afrin, Sadia creators_name: Battino, Maurizio creators_id: creators_id: creators_id: creators_id: creators_id: tamara.forbes@uneatlantico.es creators_id: creators_id: maurizio.battino@uneatlantico.es title: Guava (Psidium guajava L. cv. Red Suprema) Crude Extract Protect Human Dermal Fibroblasts against Cytotoxic Damage Mediated by Oxidative Stress ispublished: pub subjects: uneat_sn divisions: uneatlantico_produccion_cientifica full_text_status: none keywords: Guava fruit; Antioxidant capacity; Human dermal fibroblast; Oxidative stress. abstract: We analyzed guava fruits (Psidium guajava L. cv. Red Suprema) from Cuba to determine their chemical composition, total antioxidant capacity, as well as their protective effect against oxidative damage using an in vitro model of human dermal fibroblasts. The guava fruit is a natural source of bioactive compounds, such as polyphenols, vitamin C, folates and beta carotenes with proven health benefits. Human dermal fibroblasts were pre-incubated with different concentrations of guava crude extract and then subjected to oxidative stress using the AAPH stressor. The number of apoptotic and dead cells, as well as the markers of oxidative damage such as lipid and protein oxidation significantly decreased when cells were pre-incubated with guava crude extract and then exposed to the stressor. The activity of antioxidant enzymes also improved when cells were pre-incubated with guava crude extract in comparison to cells subjected to stress without prior pre-incubation with the guava extract. The results obtained in this study highlight the health benefits of guava regarding oxidative stress, proving it to be an important source of bioactive compounds associated with important biological properties. date: 2018-00-00 date_type: published publication: Plant Foods for Human Nutrition volume: 73 number: 1 pagerange: 18-24 pages: 0 id_number: doi:10.1007/s11130-018-0657-2 refereed: TRUE issn: 0921-9668 official_url: http://doi.org/10.1007/s11130-018-0657-2 num_pieces: 0 gscholar_impact: 0 gscholar_datestamp: 0000-00-00 00:00:00 citation: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica SIN ESPECIFICAR SIN ESPECIFICAR We analyzed guava fruits (Psidium guajava L. cv. Red Suprema) from Cuba to determine their chemical composition, total antioxidant capacity, as well as their protective effect against oxidative damage using an in vitro model of human dermal fibroblasts. The guava fruit is a natural source of bioactive compounds, such as polyphenols, vitamin C, folates and beta carotenes with proven health benefits. Human dermal fibroblasts were pre-incubated with different concentrations of guava crude extract and then subjected to oxidative stress using the AAPH stressor. The number of apoptotic and dead cells, as well as the markers of oxidative damage such as lipid and protein oxidation significantly decreased when cells were pre-incubated with guava crude extract and then exposed to the stressor. The activity of antioxidant enzymes also improved when cells were pre-incubated with guava crude extract in comparison to cells subjected to stress without prior pre-incubation with the guava extract. The results obtained in this study highlight the health benefits of guava regarding oxidative stress, proving it to be an important source of bioactive compounds associated with important biological properties. metadata Alvarez-Suarez, José M.; Giampieri, Francesca; Gasparrini, Massimiliano; Mazzoni, Luca; Forbes-Hernández, Tamara Y.; Afrin, Sadia y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, tamara.forbes@uneatlantico.es, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2018) Guava (Psidium guajava L. cv. Red Suprema) Crude Extract Protect Human Dermal Fibroblasts against Cytotoxic Damage Mediated by Oxidative Stress. Plant Foods for Human Nutrition, 73 (1). pp. 18-24. ISSN 0921-9668