eprintid: 7353 rev_number: 8 eprint_status: archive userid: 2 dir: disk0/00/00/73/53 datestamp: 2023-05-31 23:30:15 lastmod: 2023-05-31 23:30:15 status_changed: 2023-05-31 23:30:15 type: article metadata_visibility: show creators_name: Greco, Stefania creators_name: Pellegrino, Pamela creators_name: Giampieri, Francesca creators_name: Capocasa, Franco creators_name: Delli Carpini, Giovanni creators_name: Battino, Maurizio creators_name: Mezzetti, Bruno creators_name: Giannubilo, Stefano Raffaele creators_name: Ciavattini, Andrea creators_name: Ciarmela, Pasquapina creators_id: creators_id: creators_id: francesca.giampieri@uneatlantico.es creators_id: creators_id: creators_id: maurizio.battino@uneatlantico.es creators_id: creators_id: creators_id: creators_id: title: The In Vitro Effects of Romina Strawberry Extract on 3D Uterine Leiomyosarcoma Cells ispublished: pub subjects: uneat_sn divisions: uneatlantico_produccion_cientifica full_text_status: public keywords: leiomyosarcoma; phytochemicals; strawberry; 3D culture abstract: Leiomyosarcoma is an aggressive soft tissue sarcoma derived from the smooth muscle cells of the uterus. We tested the effect of Romina strawberry extract treatment on three-dimensional cultured uterine leiomyosarcoma cells. We established 3D cultures in agarose gel, where the cells seeded were able to form spheroids. We performed the observation and counting of the spheroids with a phase-contrast optical microscope, finding a decrease in the number of spheroids formed in the plates after 24 and 48 h treatment with 250 µg/mL of cultivar Romina strawberry extract. We also characterized the spheroids morphology by DNA binding fluorescent-stain observation, hematoxylin and eosin stain, and Masson’s trichrome stain. Finally, the real-time PCR showed a reduced expression of extracellular matrix genes after strawberry treatment. Overall, our data suggest that the fruit extract of this strawberry cultivar may be a useful therapeutic adjuvant for the management of uterine leiomyosarcoma. date: 2023 publication: Nutrients volume: 15 number: 11 pagerange: 2557 id_number: doi:10.3390/nu15112557 refereed: TRUE issn: 2072-6643 official_url: http://doi.org/10.3390/nu15112557 access: open language: en citation: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica Abierto Inglés Leiomyosarcoma is an aggressive soft tissue sarcoma derived from the smooth muscle cells of the uterus. We tested the effect of Romina strawberry extract treatment on three-dimensional cultured uterine leiomyosarcoma cells. We established 3D cultures in agarose gel, where the cells seeded were able to form spheroids. We performed the observation and counting of the spheroids with a phase-contrast optical microscope, finding a decrease in the number of spheroids formed in the plates after 24 and 48 h treatment with 250 µg/mL of cultivar Romina strawberry extract. We also characterized the spheroids morphology by DNA binding fluorescent-stain observation, hematoxylin and eosin stain, and Masson’s trichrome stain. Finally, the real-time PCR showed a reduced expression of extracellular matrix genes after strawberry treatment. Overall, our data suggest that the fruit extract of this strawberry cultivar may be a useful therapeutic adjuvant for the management of uterine leiomyosarcoma. metadata Greco, Stefania; Pellegrino, Pamela; Giampieri, Francesca; Capocasa, Franco; Delli Carpini, Giovanni; Battino, Maurizio; Mezzetti, Bruno; Giannubilo, Stefano Raffaele; Ciavattini, Andrea y Ciarmela, Pasquapina mail SIN ESPECIFICAR, SIN ESPECIFICAR, francesca.giampieri@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2023) The In Vitro Effects of Romina Strawberry Extract on 3D Uterine Leiomyosarcoma Cells. Nutrients, 15 (11). p. 2557. ISSN 2072-6643 document_url: http://repositorio.uneatlantico.es/id/eprint/7353/1/nutrients-15-02557.pdf