TY  - JOUR
UR  - http://doi.org/10.3390/nu15112557
AV  - public
TI  - The In Vitro Effects of Romina Strawberry Extract on 3D Uterine Leiomyosarcoma Cells
ID  - uneatlantico7353
IS  - 11
KW  - leiomyosarcoma; phytochemicals; strawberry; 3D culture
A1  - Greco, Stefania
A1  - Pellegrino, Pamela
A1  - Giampieri, Francesca
A1  - Capocasa, Franco
A1  - Delli Carpini, Giovanni
A1  - Battino, Maurizio
A1  - Mezzetti, Bruno
A1  - Giannubilo, Stefano Raffaele
A1  - Ciavattini, Andrea
A1  - Ciarmela, Pasquapina
Y1  - 2023///
VL  - 15
N2  - Leiomyosarcoma is an aggressive soft tissue sarcoma derived from the smooth muscle cells of the uterus. We tested the effect of Romina strawberry extract treatment on three-dimensional cultured uterine leiomyosarcoma cells. We established 3D cultures in agarose gel, where the cells seeded were able to form spheroids. We performed the observation and counting of the spheroids with a phase-contrast optical microscope, finding a decrease in the number of spheroids formed in the plates after 24 and 48 h treatment with 250 µg/mL of cultivar Romina strawberry extract. We also characterized the spheroids morphology by DNA binding fluorescent-stain observation, hematoxylin and eosin stain, and Masson?s trichrome stain. Finally, the real-time PCR showed a reduced expression of extracellular matrix genes after strawberry treatment. Overall, our data suggest that the fruit extract of this strawberry cultivar may be a useful therapeutic adjuvant for the management of uterine leiomyosarcoma.
JF  - Nutrients
SN  - 2072-6643
ER  -