eprintid: 627 rev_number: 11 eprint_status: archive userid: 2 dir: disk0/00/00/06/27 datestamp: 2022-04-13 23:55:18 lastmod: 2023-07-04 23:30:25 status_changed: 2022-04-13 23:55:18 type: article metadata_visibility: show creators_name: Cianciosi, Danila creators_name: Forbes-Hernandez, Tamara Y. creators_name: Alvarez-Suarez, José M. creators_name: Ansary, Johura creators_name: Quinzi, Denise creators_name: Amici, Adolfo creators_name: Navarro-Hortal, María D. creators_name: Esteban-Muñoz, Adelaida creators_name: Quiles, José Luis creators_name: Battino, Maurizio creators_name: Giampieri, Francesca title: Anti-inflammatory activities of Italian Chestnut and Eucalyptus honeys on murine RAW 264.7 macrophages ispublished: pub subjects: uneat_sn divisions: uneatlantico_produccion_cientifica full_text_status: none keywords: Honey; Inflammation; Mitochondrial respiration; Apoptosis; Wound healing; Antioxidant enzymes abstract: The aim of this study was to evaluate for the first time the anti-inflammatory effects of chestnut (Castanea sativa) and Eucalyptus (Eucalyptus occidentalis) honeys collected in Italy on murine RAW 264.7 macrophages stressed with the bacterial endotoxin lipopolysaccharide (LPS). Pre-treatment with the two honeys markedly decreased apoptosis and stimulated wound healing in macrophages stressed with LPS, where they also reduced the intracellular levels of ROS and nitrite. Additionally, treatment with LPS decreased the levels of glutathione and suppressed the antioxidant enzyme activities, which were significantly (p < 0.05) increased in the presence of the two honeys, through the modulation of Nrf2 expression; in LPS-stressed macrophages, Chestnut or Eucalyptus honeys protected against inflammation by regulating the main inflammatory biomarkers, including TNF-α, IL-10 and iNOS, through the decrease of NF-кB expression. Finally, they improved mitochondrial respiration and the main related parameters. date: 2021-12 publication: Journal of Functional Foods volume: 87 pagerange: 104752 id_number: doi:10.1016/j.jff.2021.104752 refereed: TRUE issn: 17564646 official_url: http://doi.org/10.1016/j.jff.2021.104752 access: open language: en citation: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica Abierto Inglés The aim of this study was to evaluate for the first time the anti-inflammatory effects of chestnut (Castanea sativa) and Eucalyptus (Eucalyptus occidentalis) honeys collected in Italy on murine RAW 264.7 macrophages stressed with the bacterial endotoxin lipopolysaccharide (LPS). Pre-treatment with the two honeys markedly decreased apoptosis and stimulated wound healing in macrophages stressed with LPS, where they also reduced the intracellular levels of ROS and nitrite. Additionally, treatment with LPS decreased the levels of glutathione and suppressed the antioxidant enzyme activities, which were significantly (p < 0.05) increased in the presence of the two honeys, through the modulation of Nrf2 expression; in LPS-stressed macrophages, Chestnut or Eucalyptus honeys protected against inflammation by regulating the main inflammatory biomarkers, including TNF-α, IL-10 and iNOS, through the decrease of NF-кB expression. Finally, they improved mitochondrial respiration and the main related parameters. metadata Cianciosi, Danila; Forbes-Hernandez, Tamara Y.; Alvarez-Suarez, José M.; Ansary, Johura; Quinzi, Denise; Amici, Adolfo; Navarro-Hortal, María D.; Esteban-Muñoz, Adelaida; Quiles, José Luis; Battino, Maurizio y Giampieri, Francesca mail SIN ESPECIFICAR (2021) Anti-inflammatory activities of Italian Chestnut and Eucalyptus honeys on murine RAW 264.7 macrophages. Journal of Functional Foods, 87. p. 104752. ISSN 17564646