eprintid: 612 rev_number: 8 eprint_status: archive userid: 2 dir: disk0/00/00/06/12 datestamp: 2022-04-13 23:55:11 lastmod: 2022-04-13 23:55:12 status_changed: 2022-04-13 23:55:11 type: article metadata_visibility: show creators_name: Giampieri, Francesca creators_name: Alvarez-Suarez, José creators_name: Mazzoni, Luca creators_name: Forbes-Hernandez, Tamara Y. creators_name: Gasparrini, Massimiliano creators_name: Gonzàlez-Paramàs, Ana creators_name: Santos-Buelga, Celestino creators_name: Quiles, José creators_name: Bompadre, Stefano creators_name: Mezzetti, Bruno creators_name: Battino, Maurizio creators_id: creators_id: creators_id: creators_id: tamara.forbes@unini.edu.mx creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: maurizio.battino@uneatlantico.es title: Polyphenol-Rich Strawberry Extract Protects Human Dermal Fibroblasts against Hydrogen Peroxide Oxidative Damage and Improves Mitochondrial Functionality ispublished: pub subjects: uneat_sn divisions: uneatlantico_produccion_cientifica divisions: uninimx_produccion_cientifica full_text_status: public keywords: strawberry; anthocyanin; ROS; lipid peroxidation; DNA damage; fibroblasts abstract: Strawberry bioactive compounds are widely known to be powerful antioxidants. In this study, the antioxidant and anti-aging activities of a polyphenol-rich strawberry extract were evaluated using human dermal fibroblasts exposed to H2O2. Firstly, the phenol and flavonoid contents of strawberry extract were studied, as well as the antioxidant capacity. HPLC-DAD analysis was performed to determine the vitamin C and β-carotene concentration, while HPLC-DAD/ESI-MS analysis was used for anthocyanin identification. Strawberry extract presented a high antioxidant capacity, and a relevant concentration of vitamins and phenolics. Pelargonidin- and cyanidin-glycosides were the most representative anthocyanin components of the fruits. Fibroblasts incubated with strawberry extract and stressed with H2O2 showed an increase in cell viability, a smaller intracellular amount of ROS, and a reduction of membrane lipid peroxidation and DNA damage. Strawberry extract was also able to improve mitochondrial functionality, increasing the basal respiration of mitochondria and to promote a regenerative capacity of cells after exposure to pro-oxidant stimuli. These findings confirm that strawberries possess antioxidant properties and provide new insights into the beneficial role of strawberry bioactive compounds on protecting skin from oxidative stress and aging. date: 2014 date_type: published publication: Molecules volume: 19 number: 6 pagerange: 7798-7816 id_number: doi:10.3390/molecules19067798 refereed: TRUE issn: 1420-3049 official_url: http://doi.org/10.3390/molecules19067798 access: open language: en citation: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica Universidad Internacional Iberoamericana México > Investigación > Producción Científica Abierto Inglés Strawberry bioactive compounds are widely known to be powerful antioxidants. In this study, the antioxidant and anti-aging activities of a polyphenol-rich strawberry extract were evaluated using human dermal fibroblasts exposed to H2O2. Firstly, the phenol and flavonoid contents of strawberry extract were studied, as well as the antioxidant capacity. HPLC-DAD analysis was performed to determine the vitamin C and β-carotene concentration, while HPLC-DAD/ESI-MS analysis was used for anthocyanin identification. Strawberry extract presented a high antioxidant capacity, and a relevant concentration of vitamins and phenolics. Pelargonidin- and cyanidin-glycosides were the most representative anthocyanin components of the fruits. Fibroblasts incubated with strawberry extract and stressed with H2O2 showed an increase in cell viability, a smaller intracellular amount of ROS, and a reduction of membrane lipid peroxidation and DNA damage. Strawberry extract was also able to improve mitochondrial functionality, increasing the basal respiration of mitochondria and to promote a regenerative capacity of cells after exposure to pro-oxidant stimuli. These findings confirm that strawberries possess antioxidant properties and provide new insights into the beneficial role of strawberry bioactive compounds on protecting skin from oxidative stress and aging. metadata Giampieri, Francesca; Alvarez-Suarez, José; Mazzoni, Luca; Forbes-Hernandez, Tamara Y.; Gasparrini, Massimiliano; Gonzàlez-Paramàs, Ana; Santos-Buelga, Celestino; Quiles, José; Bompadre, Stefano; Mezzetti, Bruno y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, tamara.forbes@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2014) Polyphenol-Rich Strawberry Extract Protects Human Dermal Fibroblasts against Hydrogen Peroxide Oxidative Damage and Improves Mitochondrial Functionality. Molecules, 19 (6). pp. 7798-7816. ISSN 1420-3049 document_url: http://repositorio.uneatlantico.es/id/eprint/612/1/molecules-19-07798.pdf