%A Francesca Giampieri
%A José Alvarez-Suarez
%A Luca Mazzoni
%A Tamara Y. Forbes-Hernandez
%A Massimiliano Gasparrini
%A Ana Gonzàlez-Paramàs
%A Celestino Santos-Buelga
%A José Quiles
%A Stefano Bompadre
%A Bruno Mezzetti
%A Maurizio Battino
%J Molecules
%K strawberry; anthocyanin; ROS; lipid peroxidation; DNA damage; fibroblasts
%N 6
%T Polyphenol-Rich Strawberry Extract Protects Human Dermal Fibroblasts against Hydrogen Peroxide Oxidative Damage and Improves Mitochondrial Functionality
%D 2014
%V 19
%P 7798-7816
%L uneatlantico612
%R doi:10.3390/molecules19067798
%X Strawberry bioactive compounds are widely known to be powerful antioxidants. In this study, the antioxidant and anti-aging activities of a polyphenol-rich strawberry extract were evaluated using human dermal fibroblasts exposed to H2O2. Firstly, the phenol and flavonoid contents of strawberry extract were studied, as well as the antioxidant capacity. HPLC-DAD analysis was performed to determine the vitamin C and β-carotene concentration, while HPLC-DAD/ESI-MS analysis was used for anthocyanin identification. Strawberry extract presented a high antioxidant capacity, and a relevant concentration of vitamins and phenolics. Pelargonidin- and cyanidin-glycosides were the most representative anthocyanin components of the fruits. Fibroblasts incubated with strawberry extract and stressed with H2O2 showed an increase in cell viability, a smaller intracellular amount of ROS, and a reduction of membrane lipid peroxidation and DNA damage. Strawberry extract was also able to improve mitochondrial functionality, increasing the basal respiration of mitochondria and to promote a regenerative capacity of cells after exposure to pro-oxidant stimuli. These findings confirm that strawberries possess antioxidant properties and provide new insights into the beneficial role of strawberry bioactive compounds on protecting skin from oxidative stress and aging.