TY  - JOUR
ID  - uneatlantico612
EP  - 7816
TI  - Polyphenol-Rich Strawberry Extract Protects Human Dermal Fibroblasts against Hydrogen Peroxide Oxidative Damage and Improves Mitochondrial Functionality
KW  - strawberry; anthocyanin; ROS; lipid peroxidation; DNA damage; fibroblasts
A1  - Giampieri, Francesca
A1  - Alvarez-Suarez, José
A1  - Mazzoni, Luca
A1  - Forbes-Hernandez, Tamara Y.
A1  - Gasparrini, Massimiliano
A1  - Gonzālez-Paramās, Ana
A1  - Santos-Buelga, Celestino
A1  - Quiles, José
A1  - Bompadre, Stefano
A1  - Mezzetti, Bruno
A1  - Battino, Maurizio
AV  - public
IS  - 6
SP  - 7798
JF  - Molecules
N2  - Strawberry bioactive compounds are widely known to be powerful antioxidants. In this study, the antioxidant and anti-aging activities of a polyphenol-rich strawberry extract were evaluated using human dermal fibroblasts exposed to H2O2. Firstly, the phenol and flavonoid contents of strawberry extract were studied, as well as the antioxidant capacity. HPLC-DAD analysis was performed to determine the vitamin C and ?-carotene concentration, while HPLC-DAD/ESI-MS analysis was used for anthocyanin identification. Strawberry extract presented a high antioxidant capacity, and a relevant concentration of vitamins and phenolics. Pelargonidin- and cyanidin-glycosides were the most representative anthocyanin components of the fruits. Fibroblasts incubated with strawberry extract and stressed with H2O2 showed an increase in cell viability, a smaller intracellular amount of ROS, and a reduction of membrane lipid peroxidation and DNA damage. Strawberry extract was also able to improve mitochondrial functionality, increasing the basal respiration of mitochondria and to promote a regenerative capacity of cells after exposure to pro-oxidant stimuli. These findings confirm that strawberries possess antioxidant properties and provide new insights into the beneficial role of strawberry bioactive compounds on protecting skin from oxidative stress and aging.
Y1  - 2014///
SN  - 1420-3049
UR  - http://doi.org/10.3390/molecules19067798
VL  - 19
ER  -