<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Effects of extremely low-frequency magnetic fields on human MDA-MB-231 breast cancer cells: proteomic characterization"^^ . "Extremely low-frequency electromagnetic fields (ELF-MF) can modify the cell viability and regulatory processes of some cell types, including breast cancer cells. Breast cancer is a multifactorial disease where a role for ELF-MF cannot be excluded. ELF-MF may influence the biological properties of breast cells through molecular mechanisms and signaling pathways that are still unclear. This study analyzed the changes in the cell viability, cellular morphology, oxidative stress response and alteration of proteomic profile in breast cancer cells (MDA-MB-231) exposed to ELF-MF (50 Hz, 1 mT for 4 h). Non-tumorigenic human breast cells (MCF-10A) were used as control cells. Exposed MDA-MB-231 breast cancer cells increased their viability and live cell number and showed a higher density and length of filopodia compared with the unexposed cells. In addition, ELF-MF induced an increase of the mitochondrial ROS levels and an alteration of mitochondrial morphology. Proteomic data analysis showed that ELF-MF altered the expression of 328 proteins in MDA-MB-231 cells and of 242 proteins in MCF-10A cells. Gene Ontology term enrichment analysis demonstrated that in both cell lines ELF-MF exposure up-regulated the genes enriched in “focal adhesion” and “mitochondrion”. The ELF-MF exposure decreased the adhesive properties of MDA-MB-231 cells and increased the migration and invasion cell abilities. At the same time, proteomic analysis, confirmed by Real Time PCR, revealed that transcription factors associated with cellular reprogramming were upregulated in MDA-MB-231 cells and downregulated in MCF-10A cells after ELF-MF exposure. MDA-MB-231 breast cancer cells exposed to 1 mT 50 Hz ELF-MF showed modifications in proteomic profile together with changes in cell viability, cellular morphology, oxidative stress response, adhesion, migration and invasion cell abilities. The main signaling pathways involved were relative to focal adhesion, mitochondrion and cellular reprogramming."^^ . "2023" . . . "253" . . "Ecotoxicology and Environmental Safety"^^ . . . "01476513" . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . "Massimo"^^ . "Bracci"^^ . "Massimo Bracci"^^ . . "Matteo"^^ . "Giulietti"^^ . "Matteo Giulietti"^^ . . "Maria Fiorella"^^ . "Tartaglione"^^ . "Maria Fiorella Tartaglione"^^ . . "Laura"^^ . "Zingaretti"^^ . "Laura Zingaretti"^^ . . "Gianluca"^^ . "Fulgenzi"^^ . "Gianluca Fulgenzi"^^ . . "Francesco"^^ . "Piva"^^ . "Francesco Piva"^^ . . "Raffaella"^^ . "Lazzarini"^^ . "Raffaella Lazzarini"^^ . . "Lory"^^ . "Santarelli"^^ . "Lory Santarelli"^^ . . "Adriano"^^ . "Tagliabracci"^^ . "Adriano Tagliabracci"^^ . . "Matteo"^^ . "Valentino"^^ . "Matteo Valentino"^^ . . "Maria"^^ . "Elexpuru Zabaleta"^^ . "Maria Elexpuru Zabaleta"^^ . . . . . . "Effects of extremely low-frequency magnetic fields on human MDA-MB-231 breast cancer cells: proteomic characterization (Texto)"^^ . . . "1-s2.0-S0147651323001549-main.pdf"^^ . . . "Effects of extremely low-frequency magnetic fields on human MDA-MB-231 breast cancer cells: proteomic characterization (Otro)"^^ . . . . . . "indexcodes.txt"^^ . . . "Effects of extremely low-frequency magnetic fields on human MDA-MB-231 breast cancer cells: proteomic characterization (Otro)"^^ . . . . . . "lightbox.jpg"^^ . . . "Effects of extremely low-frequency magnetic fields on human MDA-MB-231 breast cancer cells: proteomic characterization (Otro)"^^ . . . . . . "preview.jpg"^^ . . . "Effects of extremely low-frequency magnetic fields on human MDA-MB-231 breast cancer cells: proteomic characterization (Otro)"^^ . . . . . . "medium.jpg"^^ . . . "Effects of extremely low-frequency magnetic fields on human MDA-MB-231 breast cancer cells: proteomic characterization (Otro)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #5969 \n\nEffects of extremely low-frequency magnetic fields on human MDA-MB-231 breast cancer cells: proteomic characterization\n\n" . "text/html" . . . "Biomedicine"@en . "Biomedicina"@es . .