eprintid: 564 rev_number: 10 eprint_status: archive userid: 2 dir: disk0/00/00/05/64 datestamp: 2022-03-15 23:55:10 lastmod: 2023-07-13 23:30:17 status_changed: 2022-03-15 23:55:10 type: article metadata_visibility: show creators_name: Cianciosi, Danila creators_name: Forbes-Hernández, Tamara Y. creators_name: Regolo, Lucia creators_name: Alvarez-Suarez, José M. creators_name: Quinzi, Denise creators_name: Sargenti, Azzurra creators_name: Bai, Weibin creators_name: Tian, Lingmin creators_name: Giampieri, Francesca creators_name: Battino, Maurizio creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: francesca.giampieri@uneatlantico.es creators_id: maurizio.battino@uneatlantico.es title: Manuka honey in combination with 5-Fluorouracil decreases physical parameters of colonspheres enriched with cancer stem-like cells and reduces their resistance to apoptosis ispublished: pub subjects: uneat_sn divisions: uneatlantico_produccion_cientifica full_text_status: none keywords: Apoptosis; Cancer; Chemosensibility; Honey; Spheroids; Stemness. abstract: The aim of the present work was to evaluate the in vitro effect of Manuka honey and its combination with 5-Fu, the most common drug used in the treatment of colon cancer, on the morphological and physical parameters of colonspheres enriched with cancer stem-like cells deriving from HCT-116 colon adenocarcinoma cell line and on the apoptosis rate. Manuka honey, alone and more in combination with 5-Fu, reduced the weight, the diameter and mass density of the spheroids and induced apoptosis through the downregulation of many apoptosis inhibitors, including IAPs (Livin, Survivin, XIAP), IGFs (IGF-I, IGF-II and IGF-IR) and HSPs (HSP-27, HSP-60 and HSP-70). These results led to a reduction in the survival ability of cancer stem-like cells, as well as to a chemosensitizing effect of honey towards 5-Fu, considering that apoptosis resistance is one of the main causes of cancer stem-like cells chemoresistance. date: 2022-04 publication: Food Chemistry volume: 374 pagerange: 131753 id_number: doi:10.1016/j.foodchem.2021.131753 refereed: TRUE issn: 03088146 official_url: http://doi.org/10.1016/j.foodchem.2021.131753 access: close language: en citation: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica Cerrado Inglés The aim of the present work was to evaluate the in vitro effect of Manuka honey and its combination with 5-Fu, the most common drug used in the treatment of colon cancer, on the morphological and physical parameters of colonspheres enriched with cancer stem-like cells deriving from HCT-116 colon adenocarcinoma cell line and on the apoptosis rate. Manuka honey, alone and more in combination with 5-Fu, reduced the weight, the diameter and mass density of the spheroids and induced apoptosis through the downregulation of many apoptosis inhibitors, including IAPs (Livin, Survivin, XIAP), IGFs (IGF-I, IGF-II and IGF-IR) and HSPs (HSP-27, HSP-60 and HSP-70). These results led to a reduction in the survival ability of cancer stem-like cells, as well as to a chemosensitizing effect of honey towards 5-Fu, considering that apoptosis resistance is one of the main causes of cancer stem-like cells chemoresistance. metadata Cianciosi, Danila; Forbes-Hernández, Tamara Y.; Regolo, Lucia; Alvarez-Suarez, José M.; Quinzi, Denise; Sargenti, Azzurra; Bai, Weibin; Tian, Lingmin; Giampieri, Francesca y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, francesca.giampieri@uneatlantico.es, maurizio.battino@uneatlantico.es (2022) Manuka honey in combination with 5-Fluorouracil decreases physical parameters of colonspheres enriched with cancer stem-like cells and reduces their resistance to apoptosis. Food Chemistry, 374. p. 131753. ISSN 03088146