%D 2022
%T Manuka honey in combination with 5-Fluorouracil decreases physical parameters of colonspheres enriched with cancer stem-like cells and reduces their resistance to apoptosis
%V 374
%A Danila Cianciosi
%A Tamara Y. Forbes-Hernández
%A Lucia Regolo
%A José M. Alvarez-Suarez
%A Denise Quinzi
%A Azzurra Sargenti
%A Weibin Bai
%A Lingmin Tian
%A Francesca Giampieri
%A Maurizio Battino
%J Food Chemistry
%K Apoptosis; Cancer; Chemosensibility; Honey; Spheroids; Stemness.
%R doi:10.1016/j.foodchem.2021.131753
%X The aim of the present work was to evaluate the in vitro effect of Manuka honey and its combination with 5-Fu, the most common drug used in the treatment of colon cancer, on the morphological and physical parameters of colonspheres enriched with cancer stem-like cells deriving from HCT-116 colon adenocarcinoma cell line and on the apoptosis rate. Manuka honey, alone and more in combination with 5-Fu, reduced the weight, the diameter and mass density of the spheroids and induced apoptosis through the downregulation of many apoptosis inhibitors, including IAPs (Livin, Survivin, XIAP), IGFs (IGF-I, IGF-II and IGF-IR) and HSPs (HSP-27, HSP-60 and HSP-70). These results led to a reduction in the survival ability of cancer stem-like cells, as well as to a chemosensitizing effect of honey towards 5-Fu, considering that apoptosis resistance is one of the main causes of cancer stem-like cells chemoresistance.
%P 131753
%L uneatlantico564