%0 Journal Article
%@ 03088146
%A Cianciosi, Danila
%A Forbes-Hernández, Tamara Y.
%A Regolo, Lucia
%A Alvarez-Suarez, José M.
%A Quinzi, Denise
%A Sargenti, Azzurra
%A Bai, Weibin
%A Tian, Lingmin
%A Giampieri, Francesca
%A Battino, Maurizio
%D 2022
%F uneatlantico:564
%J Food Chemistry
%K Apoptosis; Cancer; Chemosensibility; Honey; Spheroids; Stemness.
%P 131753
%T Manuka honey in combination with 5-Fluorouracil decreases physical parameters of colonspheres enriched with cancer stem-like cells and reduces their resistance to apoptosis
%U http://repositorio.uneatlantico.es/id/eprint/564/
%V 374
%X The aim of the present work was to evaluate the in vitro effect of Manuka honey and its combination with 5-Fu, the most common drug used in the treatment of colon cancer, on the morphological and physical parameters of colonspheres enriched with cancer stem-like cells deriving from HCT-116 colon adenocarcinoma cell line and on the apoptosis rate. Manuka honey, alone and more in combination with 5-Fu, reduced the weight, the diameter and mass density of the spheroids and induced apoptosis through the downregulation of many apoptosis inhibitors, including IAPs (Livin, Survivin, XIAP), IGFs (IGF-I, IGF-II and IGF-IR) and HSPs (HSP-27, HSP-60 and HSP-70). These results led to a reduction in the survival ability of cancer stem-like cells, as well as to a chemosensitizing effect of honey towards 5-Fu, considering that apoptosis resistance is one of the main causes of cancer stem-like cells chemoresistance.