eprintid: 5528 rev_number: 8 eprint_status: archive userid: 2 dir: disk0/00/00/55/28 datestamp: 2023-01-23 23:30:05 lastmod: 2023-01-23 23:30:06 status_changed: 2023-01-23 23:30:05 type: article metadata_visibility: show creators_name: Pirković, Andrea creators_name: Vilotić, Aleksandra creators_name: Borozan, Sunčica creators_name: Nacka-Aleksić, Mirjana creators_name: Bojić-Trbojević, Žanka creators_name: Krivokuća, Milica Jovanović creators_name: Battino, Maurizio creators_name: Giampieri, Francesca creators_name: Dekanski, Dragana creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: maurizio.battino@uneatlantico.es creators_id: francesca.giampieri@uneatlantico.es creators_id: title: Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells ispublished: pub subjects: uneat_sn divisions: uneatlantico_produccion_cientifica full_text_status: public keywords: oleuropein; trophoblast; oxidative stress; pregnancy abstract: Olive-derived bioactive compound oleuropein was evaluated against damage induced by hydrogen peroxide in human trophoblast cells in vitro, by examining the changes in several markers implicated in oxidative stress interactions in the placenta. Trophoblast HTR-8/SVneo cells were preincubated with OLE at 10 and 100 µM and exposed to H2O2, as a model of oxidative stress. Protein and lipid peroxidation, as well as antioxidant enzymes’ activity, were determined spectrophotometrically, and DNA damage was evaluated by comet assay. iNOS protein expression was assessed by Western blot, while the mRNA expression of pro- and anti-apoptotic genes BAX and BCL2 and transcription factor NFE2L2, as well as cytokines IL-6 and TNF α were determined by qPCR. Oleuropein demonstrated cytoprotective effects against H2O2 in trophoblast cells by significantly improving the antioxidant status and preventing protein and lipid damage, as well as reducing the iNOS levels. OLE reduced the mRNA expression of IL-6 and TNF α, however, it did not influence the expression of NFE2L2 or the BAX/BCL2 ratio after H2O2 exposure. Oleuropein per se did not lead to any adverse effects in HTR-8/SVneo cells under the described conditions, confirming its safety in vitro. In conclusion, it significantly attenuated oxidative damage and restored antioxidant functioning, confirming its protective role in trophoblast date: 2023 publication: Antioxidants volume: 12 number: 1 pagerange: 197 id_number: doi:10.3390/antiox12010197 refereed: TRUE issn: 2076-3921 official_url: http://doi.org/10.3390/antiox12010197 access: open language: en citation: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Producción Científica Abierto Inglés Olive-derived bioactive compound oleuropein was evaluated against damage induced by hydrogen peroxide in human trophoblast cells in vitro, by examining the changes in several markers implicated in oxidative stress interactions in the placenta. Trophoblast HTR-8/SVneo cells were preincubated with OLE at 10 and 100 µM and exposed to H2O2, as a model of oxidative stress. Protein and lipid peroxidation, as well as antioxidant enzymes’ activity, were determined spectrophotometrically, and DNA damage was evaluated by comet assay. iNOS protein expression was assessed by Western blot, while the mRNA expression of pro- and anti-apoptotic genes BAX and BCL2 and transcription factor NFE2L2, as well as cytokines IL-6 and TNF α were determined by qPCR. Oleuropein demonstrated cytoprotective effects against H2O2 in trophoblast cells by significantly improving the antioxidant status and preventing protein and lipid damage, as well as reducing the iNOS levels. OLE reduced the mRNA expression of IL-6 and TNF α, however, it did not influence the expression of NFE2L2 or the BAX/BCL2 ratio after H2O2 exposure. Oleuropein per se did not lead to any adverse effects in HTR-8/SVneo cells under the described conditions, confirming its safety in vitro. In conclusion, it significantly attenuated oxidative damage and restored antioxidant functioning, confirming its protective role in trophoblast metadata Pirković, Andrea; Vilotić, Aleksandra; Borozan, Sunčica; Nacka-Aleksić, Mirjana; Bojić-Trbojević, Žanka; Krivokuća, Milica Jovanović; Battino, Maurizio; Giampieri, Francesca y Dekanski, Dragana mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es, francesca.giampieri@uneatlantico.es, SIN ESPECIFICAR (2023) Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells. Antioxidants, 12 (1). p. 197. ISSN 2076-3921 document_url: http://repositorio.uneatlantico.es/id/eprint/5528/1/antioxidants-12-00197-v3.pdf