<> "The repository administrator has not yet configured an RDF license."^^ . <> . . . "Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells"^^ . "Olive-derived bioactive compound oleuropein was evaluated against damage induced by hydrogen peroxide in human trophoblast cells in vitro, by examining the changes in several markers implicated in oxidative stress interactions in the placenta. Trophoblast HTR-8/SVneo cells were preincubated with OLE at 10 and 100 µM and exposed to H2O2, as a model of oxidative stress. Protein and lipid peroxidation, as well as antioxidant enzymes’ activity, were determined spectrophotometrically, and DNA damage was evaluated by comet assay. iNOS protein expression was assessed by Western blot, while the mRNA expression of pro- and anti-apoptotic genes BAX and BCL2 and transcription factor NFE2L2, as well as cytokines IL-6 and TNF α were determined by qPCR. Oleuropein demonstrated cytoprotective effects against H2O2 in trophoblast cells by significantly improving the antioxidant status and preventing protein and lipid damage, as well as reducing the iNOS levels. OLE reduced the mRNA expression of IL-6 and TNF α, however, it did not influence the expression of NFE2L2 or the BAX/BCL2 ratio after H2O2 exposure. Oleuropein per se did not lead to any adverse effects in HTR-8/SVneo cells under the described conditions, confirming its safety in vitro. In conclusion, it significantly attenuated oxidative damage and restored antioxidant functioning, confirming its protective role in trophoblast"^^ . "2023" . . . "12" . "1" . . "Antioxidants"^^ . . . "20763921" . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . "Aleksandra"^^ . "Vilotić"^^ . "Aleksandra Vilotić"^^ . . "Milica Jovanović"^^ . "Krivokuća"^^ . "Milica Jovanović Krivokuća"^^ . . "Mirjana"^^ . "Nacka-Aleksić"^^ . "Mirjana Nacka-Aleksić"^^ . . "Maurizio"^^ . "Battino"^^ . "Maurizio Battino"^^ . . "Andrea"^^ . "Pirković"^^ . "Andrea Pirković"^^ . . "Francesca"^^ . "Giampieri"^^ . "Francesca Giampieri"^^ . . "Dragana"^^ . "Dekanski"^^ . "Dragana Dekanski"^^ . . "Sunčica"^^ . "Borozan"^^ . "Sunčica Borozan"^^ . . "Žanka"^^ . "Bojić-Trbojević"^^ . "Žanka Bojić-Trbojević"^^ . . . . . . "Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells (Texto)"^^ . . . "antioxidants-12-00197-v3.pdf"^^ . . . "Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells (Otro)"^^ . . . . . . "indexcodes.txt"^^ . . . "Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells (Otro)"^^ . . . . . . "lightbox.jpg"^^ . . . "Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells (Otro)"^^ . . . . . . "preview.jpg"^^ . . . "Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells (Otro)"^^ . . . . . . "medium.jpg"^^ . . . "Oleuropein Attenuates Oxidative Stress in Human Trophoblast Cells (Otro)"^^ . . . . . . "small.jpg"^^ . . "HTML Summary of #5528 \n\nOleuropein Attenuates Oxidative Stress in Human Trophoblast Cells\n\n" . "text/html" . . . "Nutrition"@en . "Alimentación"@es . .