eprintid: 4691 rev_number: 11 eprint_status: archive userid: 2 dir: disk0/00/00/46/91 datestamp: 2022-11-21 23:30:05 lastmod: 2023-07-10 23:30:34 status_changed: 2022-11-21 23:30:05 type: article metadata_visibility: show creators_name: Elexpuru Zabaleta, Maria creators_name: Lazzarini, Raffaella creators_name: Tartaglione, Maria Fiorella creators_name: Piva, Francesco creators_name: Ciarapica, Veronica creators_name: Marinelli Busilacchi, Elena creators_name: Poloni, Antonella creators_name: Valentino, Matteo creators_name: Santarelli, Lory creators_name: Bracci, Massimo creators_id: maria.elexpuru@uneatlantico.es creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: title: A 50 Hz magnetic field influences the viability of breast cancer cells 96 h after exposure ispublished: pub subjects: uneat_bm divisions: uneatlantico_produccion_cientifica full_text_status: public keywords: Extremely low frequency magnetic feld; Breast cancer; Cell viability; MDA-MB-231; MCF-7; MCF-10A abstract: Background The exposure of breast cancer to extremely low frequency magnetic fields (ELF-MFs) results in various biological responses. Some studies have suggested a possible cancer-enhancing effect, while others showed a possible therapeutic role. This study investigated the effects of in vitro exposure to 50 Hz ELF-MF for up to 24 h on the viability and cellular response of MDA-MB-231 and MCF-7 breast cancer cell lines and MCF-10A breast cell line. Methods and results The breast cell lines were exposed to 50 Hz ELF-MF at flux densities of 0.1 mT and 1.0 mT and were examined 96 h after the beginning of ELF-MF exposure. The duration of 50 Hz ELF-MF exposure influenced the cell viability and proliferation of both the tumor and nontumorigenic breast cell lines. In particular, short-term exposure (4–8 h, 0.1 mT and 1.0 mT) led to an increase in viability in breast cancer cells, while long and high exposure (24 h, 1.0 mT) led to a decrease in viability and proliferation in all cell lines. Cancer and normal breast cells exhibited different responses to ELF-MF. Mitochondrial membrane potential and reactive oxygen species (ROS) production were altered after ELF-MF exposure, suggesting that the mitochondria are a probable target of ELF-MF in breast cells. Conclusions The viability of breast cells in vitro is influenced by ELF-MF exposure at magnetic flux densities compatible with the limits for the general population and for workplace exposures. The effects are apparent after 96 h and are related to the ELF-MF exposure time. date: 2023-02 publication: Molecular Biology Reports id_number: doi:10.1007/s11033-022-08069-7 refereed: TRUE issn: 0301-4851 official_url: http://doi.org/10.1007/s11033-022-08069-7 access: open language: en citation: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Producción Científica Abierto Inglés Background The exposure of breast cancer to extremely low frequency magnetic fields (ELF-MFs) results in various biological responses. Some studies have suggested a possible cancer-enhancing effect, while others showed a possible therapeutic role. This study investigated the effects of in vitro exposure to 50 Hz ELF-MF for up to 24 h on the viability and cellular response of MDA-MB-231 and MCF-7 breast cancer cell lines and MCF-10A breast cell line. Methods and results The breast cell lines were exposed to 50 Hz ELF-MF at flux densities of 0.1 mT and 1.0 mT and were examined 96 h after the beginning of ELF-MF exposure. The duration of 50 Hz ELF-MF exposure influenced the cell viability and proliferation of both the tumor and nontumorigenic breast cell lines. In particular, short-term exposure (4–8 h, 0.1 mT and 1.0 mT) led to an increase in viability in breast cancer cells, while long and high exposure (24 h, 1.0 mT) led to a decrease in viability and proliferation in all cell lines. Cancer and normal breast cells exhibited different responses to ELF-MF. Mitochondrial membrane potential and reactive oxygen species (ROS) production were altered after ELF-MF exposure, suggesting that the mitochondria are a probable target of ELF-MF in breast cells. Conclusions The viability of breast cells in vitro is influenced by ELF-MF exposure at magnetic flux densities compatible with the limits for the general population and for workplace exposures. The effects are apparent after 96 h and are related to the ELF-MF exposure time. metadata Elexpuru Zabaleta, Maria; Lazzarini, Raffaella; Tartaglione, Maria Fiorella; Piva, Francesco; Ciarapica, Veronica; Marinelli Busilacchi, Elena; Poloni, Antonella; Valentino, Matteo; Santarelli, Lory y Bracci, Massimo mail maria.elexpuru@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2023) A 50 Hz magnetic field influences the viability of breast cancer cells 96 h after exposure. Molecular Biology Reports. ISSN 0301-4851 document_url: http://repositorio.uneatlantico.es/id/eprint/4691/1/s11033-022-08069-7.pdf