relation: http://repositorio.uneatlantico.es/id/eprint/4691/ canonical: http://repositorio.uneatlantico.es/id/eprint/4691/ title: A 50 Hz magnetic field influences the viability of breast cancer cells 96 h after exposure creator: Elexpuru Zabaleta, Maria creator: Lazzarini, Raffaella creator: Tartaglione, Maria Fiorella creator: Piva, Francesco creator: Ciarapica, Veronica creator: Marinelli Busilacchi, Elena creator: Poloni, Antonella creator: Valentino, Matteo creator: Santarelli, Lory creator: Bracci, Massimo subject: Biomedicina description: Background The exposure of breast cancer to extremely low frequency magnetic fields (ELF-MFs) results in various biological responses. Some studies have suggested a possible cancer-enhancing effect, while others showed a possible therapeutic role. This study investigated the effects of in vitro exposure to 50 Hz ELF-MF for up to 24 h on the viability and cellular response of MDA-MB-231 and MCF-7 breast cancer cell lines and MCF-10A breast cell line. Methods and results The breast cell lines were exposed to 50 Hz ELF-MF at flux densities of 0.1 mT and 1.0 mT and were examined 96 h after the beginning of ELF-MF exposure. The duration of 50 Hz ELF-MF exposure influenced the cell viability and proliferation of both the tumor and nontumorigenic breast cell lines. In particular, short-term exposure (4–8 h, 0.1 mT and 1.0 mT) led to an increase in viability in breast cancer cells, while long and high exposure (24 h, 1.0 mT) led to a decrease in viability and proliferation in all cell lines. Cancer and normal breast cells exhibited different responses to ELF-MF. Mitochondrial membrane potential and reactive oxygen species (ROS) production were altered after ELF-MF exposure, suggesting that the mitochondria are a probable target of ELF-MF in breast cells. Conclusions The viability of breast cells in vitro is influenced by ELF-MF exposure at magnetic flux densities compatible with the limits for the general population and for workplace exposures. The effects are apparent after 96 h and are related to the ELF-MF exposure time. date: 2023-02 type: Artículo type: PeerReviewed format: text language: es identifier: http://repositorio.uneatlantico.es/id/eprint/4691/1/s11033-022-08069-7.pdf identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Producción Científica Abierto Inglés Background The exposure of breast cancer to extremely low frequency magnetic fields (ELF-MFs) results in various biological responses. Some studies have suggested a possible cancer-enhancing effect, while others showed a possible therapeutic role. This study investigated the effects of in vitro exposure to 50 Hz ELF-MF for up to 24 h on the viability and cellular response of MDA-MB-231 and MCF-7 breast cancer cell lines and MCF-10A breast cell line. Methods and results The breast cell lines were exposed to 50 Hz ELF-MF at flux densities of 0.1 mT and 1.0 mT and were examined 96 h after the beginning of ELF-MF exposure. The duration of 50 Hz ELF-MF exposure influenced the cell viability and proliferation of both the tumor and nontumorigenic breast cell lines. In particular, short-term exposure (4–8 h, 0.1 mT and 1.0 mT) led to an increase in viability in breast cancer cells, while long and high exposure (24 h, 1.0 mT) led to a decrease in viability and proliferation in all cell lines. Cancer and normal breast cells exhibited different responses to ELF-MF. Mitochondrial membrane potential and reactive oxygen species (ROS) production were altered after ELF-MF exposure, suggesting that the mitochondria are a probable target of ELF-MF in breast cells. Conclusions The viability of breast cells in vitro is influenced by ELF-MF exposure at magnetic flux densities compatible with the limits for the general population and for workplace exposures. The effects are apparent after 96 h and are related to the ELF-MF exposure time. metadata Elexpuru Zabaleta, Maria; Lazzarini, Raffaella; Tartaglione, Maria Fiorella; Piva, Francesco; Ciarapica, Veronica; Marinelli Busilacchi, Elena; Poloni, Antonella; Valentino, Matteo; Santarelli, Lory y Bracci, Massimo mail maria.elexpuru@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2023) A 50 Hz magnetic field influences the viability of breast cancer cells 96 h after exposure. Molecular Biology Reports. ISSN 0301-4851 relation: http://doi.org/10.1007/s11033-022-08069-7 relation: doi:10.1007/s11033-022-08069-7 language: en