eprintid: 28571 rev_number: 8 eprint_status: archive userid: 2 dir: disk0/00/02/85/71 datestamp: 2026-05-14 23:30:15 lastmod: 2026-05-14 23:30:16 status_changed: 2026-05-14 23:30:15 type: article metadata_visibility: show creators_name: Cianciosi, Danila creators_name: Armas Diaz, Yasmany creators_name: Yang, Bei creators_name: Qi, Zexiu creators_name: Chen, Ge creators_name: Quiles, José L. creators_name: Gasparrini, Massimiliano creators_name: Cassotta, Manuela creators_name: Calderón Iglesias, Rubén creators_name: Battino, Maurizio creators_name: Giampieri, Francesca creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: jose.quiles@uneatlantico.es creators_id: creators_id: manucassotta@gmail.com creators_id: ruben.calderon@uneatlantico.es creators_id: maurizio.battino@uneatlantico.es creators_id: francesca.giampieri@uneatlantico.es title: Liquorice alters adipocyte–breast cancer cell crosstalk by modulating oxidative stress and suppressing aromatase and renin–angiotensin signalling ispublished: pub subjects: uneat_bm subjects: uneat_sn divisions: uneatlantico_produccion_cientifica divisions: unic_produccion_cientifica divisions: uniromana_produccion_cientifica full_text_status: none keywords: besity breast cancer tumour microenvironment adipocytes liquorice adipokines abstract: Obesity is recognised to be a risk factor for breast cancer since adipose tissue influences the tumour microenvironment. This study aims to investigate the effect of the secretome of 3T3-L1 adipocytes untreated or treated with liquorice root extract (LRE), containing flavonoids, phenolic acids, and saponins on MCF-7 breast cancer cells. By treating adipocytes with LRE, the secretion of certain pro-tumorigenic factors like IGFBP-6, resistin, and VEGF was reduced. MCF-7 cells exposed to conditioned medium from LRE-treated adipocytes exhibited an increase in reactive oxygen species levels, downregulation of the Nrf2 antioxidant pathway, and increased autophagy. Those conditions reduced cell viability, migration, and colony formation. Additionally, there was downregulation of genes associated with oestrogen signalling and tumour-related processes, including CYP19A1 (aromatase), ERα, Her2, and components of the renin–angiotensin system (RAS). These findings suggest that LRE can modulate the adipocyte secretome to influence breast cancer cell behaviour under obesity-related in vitro conditions. date: 2026-05 publication: International Journal of Food Sciences and Nutrition pagerange: 1-24 id_number: doi:10.1080/09637486.2026.2666404 refereed: TRUE issn: 0963-7486 official_url: http://doi.org/10.1080/09637486.2026.2666404 access: close language: en citation: Artículo Materias > Biomedicina Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional do Cuanza > Investigación > Producción Científica Universidad de La Romana > Investigación > Producción Científica Cerrado Inglés Obesity is recognised to be a risk factor for breast cancer since adipose tissue influences the tumour microenvironment. This study aims to investigate the effect of the secretome of 3T3-L1 adipocytes untreated or treated with liquorice root extract (LRE), containing flavonoids, phenolic acids, and saponins on MCF-7 breast cancer cells. By treating adipocytes with LRE, the secretion of certain pro-tumorigenic factors like IGFBP-6, resistin, and VEGF was reduced. MCF-7 cells exposed to conditioned medium from LRE-treated adipocytes exhibited an increase in reactive oxygen species levels, downregulation of the Nrf2 antioxidant pathway, and increased autophagy. Those conditions reduced cell viability, migration, and colony formation. Additionally, there was downregulation of genes associated with oestrogen signalling and tumour-related processes, including CYP19A1 (aromatase), ERα, Her2, and components of the renin–angiotensin system (RAS). These findings suggest that LRE can modulate the adipocyte secretome to influence breast cancer cell behaviour under obesity-related in vitro conditions. metadata Cianciosi, Danila; Armas Diaz, Yasmany; Yang, Bei; Qi, Zexiu; Chen, Ge; Quiles, José L.; Gasparrini, Massimiliano; Cassotta, Manuela; Calderón Iglesias, Rubén; Battino, Maurizio y Giampieri, Francesca mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, jose.quiles@uneatlantico.es, SIN ESPECIFICAR, manucassotta@gmail.com, ruben.calderon@uneatlantico.es, maurizio.battino@uneatlantico.es, francesca.giampieri@uneatlantico.es (2026) Liquorice alters adipocyte–breast cancer cell crosstalk by modulating oxidative stress and suppressing aromatase and renin–angiotensin signalling. International Journal of Food Sciences and Nutrition. pp. 1-24. ISSN 0963-7486