eprintid: 27827 rev_number: 8 eprint_status: archive userid: 2 dir: disk0/00/02/78/27 datestamp: 2026-03-13 23:30:14 lastmod: 2026-03-13 23:30:15 status_changed: 2026-03-13 23:30:14 type: article metadata_visibility: show creators_name: Wu, Qian creators_name: Zhang, Xingyu creators_name: Zhang, Jingjia creators_name: Huang, Gaohui creators_name: Zhou, Chen creators_name: Li, Chunlin creators_name: Huang, Xiaojun creators_name: Xiao, Jianbo creators_name: Feng, Nianjie creators_name: She, Yuanbin title: Constraint of Lignin–Carbohydrate Complex Orchestrated on Polyphenol in Oil–Water Interface Targeting Ulcerative Colitis Therapy ispublished: pub subjects: uneat_bm divisions: uneatlantico_produccion_cientifica full_text_status: public keywords: intestinal microflora| lignin–carbohydrate complex| polyphenols| ulcerative colitis| W 1 /O/W 2 emulsion abstract: The therapeutic potential of polyphenols in ulcerative colitis (UC), mediated through immune modulation and gut microbiota homeostasis. To enhance the oral bioavailability of polyphenols, we architected a colon–targeted W1/O/W2 emulsion system featuring a rationally designed lignin–carbohydrate complex (LCC) as a dual–functional emulsifier system for the first time. Based on the innate structural duality of LCC, which comprising hydrophobic lignin and hydrophilic carbohydrates, we employed LCC for O/W emulsifier. This inherent amphiphilicity was further engineered via laccase–mediated grafting of isovanillin, yielding a modified LCC with tailored lipophilicity for effective W/O interfacial stabilization. The W1/O/W2 emulsion ensured the stability of the encapsulated polyphenols with divergent polarity but also enabled pH–responsive payload release under colonic conditions (pH >7.0). In DSS–induced colitis, the system demonstrated a synergistic effect, the LCC itself acted as a prebiotic to modulate the gut microbiota, specifically enriching short chain fatty acid–producing bacteria, while the released polyphenols reinforced the intestinal barrier, which collectively accelerated mucosal healing. This research proposes a carbon–neutral therapeutic strategy for colitis, not only establishing a proof–of–concept for replacing synthetic emulsifiers with engineered biomass, but also as a multi–functional platform to stabilize colon–targeted co–delivery system and microbiome regulation in colitis. date: 2026-03 publication: Advanced Science id_number: doi:10.1002/advs.202524070 refereed: TRUE issn: 2198-3844 official_url: http://doi.org/10.1002/advs.202524070 access: open language: en citation: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Abierto Inglés The therapeutic potential of polyphenols in ulcerative colitis (UC), mediated through immune modulation and gut microbiota homeostasis. To enhance the oral bioavailability of polyphenols, we architected a colon–targeted W1/O/W2 emulsion system featuring a rationally designed lignin–carbohydrate complex (LCC) as a dual–functional emulsifier system for the first time. Based on the innate structural duality of LCC, which comprising hydrophobic lignin and hydrophilic carbohydrates, we employed LCC for O/W emulsifier. This inherent amphiphilicity was further engineered via laccase–mediated grafting of isovanillin, yielding a modified LCC with tailored lipophilicity for effective W/O interfacial stabilization. The W1/O/W2 emulsion ensured the stability of the encapsulated polyphenols with divergent polarity but also enabled pH–responsive payload release under colonic conditions (pH >7.0). In DSS–induced colitis, the system demonstrated a synergistic effect, the LCC itself acted as a prebiotic to modulate the gut microbiota, specifically enriching short chain fatty acid–producing bacteria, while the released polyphenols reinforced the intestinal barrier, which collectively accelerated mucosal healing. This research proposes a carbon–neutral therapeutic strategy for colitis, not only establishing a proof–of–concept for replacing synthetic emulsifiers with engineered biomass, but also as a multi–functional platform to stabilize colon–targeted co–delivery system and microbiome regulation in colitis. metadata Wu, Qian; Zhang, Xingyu; Zhang, Jingjia; Huang, Gaohui; Zhou, Chen; Li, Chunlin; Huang, Xiaojun; Xiao, Jianbo; Feng, Nianjie y She, Yuanbin mail SIN ESPECIFICAR (2026) Constraint of Lignin–Carbohydrate Complex Orchestrated on Polyphenol in Oil–Water Interface Targeting Ulcerative Colitis Therapy. Advanced Science. ISSN 2198-3844 document_url: http://repositorio.uneatlantico.es/id/eprint/27827/1/Advanced%20Science%20-%202026%20-%20Wu%20-%20Constraint%20of%20Lignin%20Carbohydrate%20Complex%20Orchestrated%20on%20Polyphenol%20in%20Oil%20Water%20Interface.pdf