TY  - JOUR
Y1  - 2022/07//
JF  - Acta Veterinaria Hungarica
N2  - Yersiniosis, caused by the fish pathogen Yersinia ruckeri, is a serious bacterial septicaemia affecting mainly salmonids worldwide. The acute infection may result in high mortality without apparent external disease signs, while the chronic one causes moderate to considerable mortality. Survivors of yersiniosis outbreaks become carriers. Y. ruckeri is able to adhere to, and to invade, phagocytic and non-phagocytic fish cells by using unknown molecular mechanisms. The aim of this study was to describe the kinetics of cell invasion by Y. ruckeri serotype O1 biotype 1 in a fish cell line (RTG-2) originating from rainbow trout gonads. The efficiency of invasion by Y. ruckeri was found to be temperature dependent, having a maximum at 20?°C. The bacterium was able to survive up to 96?h postinfection. The incubation of the cells at 4?°C and the pre-incubation of the bacteria with sugars or heat-inactivated antiserum significantly decreased the efficiency of invasion or even completely prevented the invasion of RTG-2 cells. These findings indicate that Y. ruckeri is capable of adhering to, entering and surviving within non-phagocytic cells, and that the intracellular environment may constitute a suitable niche for this pathogen that can favour the spread of infection and/or the maintenance of a carrier state of fish.
UR  - http://doi.org/10.1556/004.2022.00013
SN  - 0236-6290
TI  - Kinetics of the invasion of a non-phagocytic fish cell line, RTG-2 by Yersinia ruckeri serotype O1 biotype 1
ID  - uneatlantico2473
KW  - Yersinia ruckeri; fish disease; cell invasion; non-phagocytic cells; carrier state
AV  - none
A1  - Padilla, Daniel
A1  - Acosta Hernández, Begoña
A1  - Ramos Vivas, Jose
A1  - Déniz, Soraya
A1  - Rosario, Inmaculada
A1  - Martín Barrasa, José Luís
A1  - Henao, Andrés sánchez
A1  - Silva Sergent, Freddy
A1  - Ramos Sosa, María josé
A1  - García Álvarez, Natalia
A1  - Real, Fernando
ER  -