eprintid: 17867 rev_number: 6 eprint_status: archive userid: 2 dir: disk0/00/01/78/67 datestamp: 2025-11-03 23:30:09 lastmod: 2025-11-03 23:30:09 status_changed: 2025-11-03 23:30:09 type: article metadata_visibility: show creators_name: Cassotta, Manuela creators_name: Armas Diaz, Yasmany creators_name: Cianciosi, Danila creators_name: Yang, Bei creators_name: Qi, Zexiu creators_name: Chen, Ge creators_name: Gracia Villar, Santos creators_name: Dzul López, Luis Alonso creators_name: Grosso, Giuseppe creators_name: Quiles, José L. creators_name: Xiao, Jianbo creators_name: Battino, Maurizio creators_name: Giampieri, Francesca creators_id: manucassotta@gmail.com creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: santos.gracia@uneatlantico.es creators_id: luis.dzul@uneatlantico.es creators_id: creators_id: creators_id: creators_id: maurizio.battino@uneatlantico.es creators_id: francesca.giampieri@uneatlantico.es title: Single-cell omics for nutrition research: an emerging opportunity for human-centric investigations ispublished: pub subjects: uneat_sn divisions: uneatlantico_produccion_cientifica divisions: uninimx_produccion_cientifica divisions: unic_produccion_cientifica divisions: uniromana_produccion_cientifica full_text_status: none keywords: Nutrition research; organoids; personalized nutrition; 3Rs; single-cell omics abstract: Understanding how dietary compounds affect human health is challenged by their molecular complexity and cell-type–specific effects. Conventional multi-cell type (bulk) analyses obscure cellular heterogeneity, while animal and standard in vitro models often fail to replicate human physiology. Single-cell omics technologies—such as single-cell RNA sequencing, as well as single-cell–resolved proteomic and metabolomic approaches—enable high-resolution investigation of nutrient–cell interactions and reveal mechanisms at a single-cell resolution. When combined with advanced human-derived in vitro systems like organoids and organ-on-chip platforms, they support mechanistic studies in physiologically relevant contexts. This review outlines emerging applications of single-cell omics in nutrition research, emphasizing their potential to uncover cell-specific dietary responses, identify nutrient-sensitive pathways, and capture interindividual variability. It also discusses key challenges—including technical limitations, model selection, and institutional biases—and identifies strategic directions to facilitate broader adoption in the field. Collectively, single-cell omics offer a transformative framework to advance human-centric nutrition research. date: 2025-11 publication: Critical Reviews in Food Science and Nutrition pagerange: 1-15 id_number: doi:10.1080/10408398.2025.2566387 refereed: TRUE issn: 1040-8398 official_url: http://doi.org/10.1080/10408398.2025.2566387 access: close language: en citation: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica Universidad Internacional do Cuanza > Investigación > Producción Científica Universidad de La Romana > Investigación > Producción Científica Cerrado Inglés Understanding how dietary compounds affect human health is challenged by their molecular complexity and cell-type–specific effects. Conventional multi-cell type (bulk) analyses obscure cellular heterogeneity, while animal and standard in vitro models often fail to replicate human physiology. Single-cell omics technologies—such as single-cell RNA sequencing, as well as single-cell–resolved proteomic and metabolomic approaches—enable high-resolution investigation of nutrient–cell interactions and reveal mechanisms at a single-cell resolution. When combined with advanced human-derived in vitro systems like organoids and organ-on-chip platforms, they support mechanistic studies in physiologically relevant contexts. This review outlines emerging applications of single-cell omics in nutrition research, emphasizing their potential to uncover cell-specific dietary responses, identify nutrient-sensitive pathways, and capture interindividual variability. It also discusses key challenges—including technical limitations, model selection, and institutional biases—and identifies strategic directions to facilitate broader adoption in the field. Collectively, single-cell omics offer a transformative framework to advance human-centric nutrition research. metadata Cassotta, Manuela; Armas Diaz, Yasmany; Cianciosi, Danila; Yang, Bei; Qi, Zexiu; Chen, Ge; Gracia Villar, Santos; Dzul López, Luis Alonso; Grosso, Giuseppe; Quiles, José L.; Xiao, Jianbo; Battino, Maurizio y Giampieri, Francesca mail manucassotta@gmail.com, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, santos.gracia@uneatlantico.es, luis.dzul@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es, francesca.giampieri@uneatlantico.es (2025) Single-cell omics for nutrition research: an emerging opportunity for human-centric investigations. Critical Reviews in Food Science and Nutrition. pp. 1-15. ISSN 1040-8398