relation: http://repositorio.uneatlantico.es/id/eprint/17867/ canonical: http://repositorio.uneatlantico.es/id/eprint/17867/ title: Single-cell omics for nutrition research: an emerging opportunity for human-centric investigations creator: Cassotta, Manuela creator: Armas Diaz, Yasmany creator: Cianciosi, Danila creator: Yang, Bei creator: Qi, Zexiu creator: Chen, Ge creator: Gracia Villar, Santos creator: Dzul López, Luis Alonso creator: Grosso, Giuseppe creator: Quiles, José L. creator: Xiao, Jianbo creator: Battino, Maurizio creator: Giampieri, Francesca subject: Alimentación description: Understanding how dietary compounds affect human health is challenged by their molecular complexity and cell-type–specific effects. Conventional multi-cell type (bulk) analyses obscure cellular heterogeneity, while animal and standard in vitro models often fail to replicate human physiology. Single-cell omics technologies—such as single-cell RNA sequencing, as well as single-cell–resolved proteomic and metabolomic approaches—enable high-resolution investigation of nutrient–cell interactions and reveal mechanisms at a single-cell resolution. When combined with advanced human-derived in vitro systems like organoids and organ-on-chip platforms, they support mechanistic studies in physiologically relevant contexts. This review outlines emerging applications of single-cell omics in nutrition research, emphasizing their potential to uncover cell-specific dietary responses, identify nutrient-sensitive pathways, and capture interindividual variability. It also discusses key challenges—including technical limitations, model selection, and institutional biases—and identifies strategic directions to facilitate broader adoption in the field. Collectively, single-cell omics offer a transformative framework to advance human-centric nutrition research. date: 2025-11 type: Artículo type: PeerReviewed identifier: Artículo Materias > Alimentación Universidad Europea del Atlántico > Investigación > Artículos y libros Universidad Internacional Iberoamericana México > Investigación > Producción Científica Universidad Internacional do Cuanza > Investigación > Producción Científica Universidad de La Romana > Investigación > Producción Científica Cerrado Inglés Understanding how dietary compounds affect human health is challenged by their molecular complexity and cell-type–specific effects. Conventional multi-cell type (bulk) analyses obscure cellular heterogeneity, while animal and standard in vitro models often fail to replicate human physiology. Single-cell omics technologies—such as single-cell RNA sequencing, as well as single-cell–resolved proteomic and metabolomic approaches—enable high-resolution investigation of nutrient–cell interactions and reveal mechanisms at a single-cell resolution. When combined with advanced human-derived in vitro systems like organoids and organ-on-chip platforms, they support mechanistic studies in physiologically relevant contexts. This review outlines emerging applications of single-cell omics in nutrition research, emphasizing their potential to uncover cell-specific dietary responses, identify nutrient-sensitive pathways, and capture interindividual variability. It also discusses key challenges—including technical limitations, model selection, and institutional biases—and identifies strategic directions to facilitate broader adoption in the field. Collectively, single-cell omics offer a transformative framework to advance human-centric nutrition research. metadata Cassotta, Manuela; Armas Diaz, Yasmany; Cianciosi, Danila; Yang, Bei; Qi, Zexiu; Chen, Ge; Gracia Villar, Santos; Dzul López, Luis Alonso; Grosso, Giuseppe; Quiles, José L.; Xiao, Jianbo; Battino, Maurizio y Giampieri, Francesca mail manucassotta@gmail.com, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, santos.gracia@uneatlantico.es, luis.dzul@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es, francesca.giampieri@uneatlantico.es (2025) Single-cell omics for nutrition research: an emerging opportunity for human-centric investigations. Critical Reviews in Food Science and Nutrition. pp. 1-15. ISSN 1040-8398 relation: http://doi.org/10.1080/10408398.2025.2566387 relation: doi:10.1080/10408398.2025.2566387 language: en