@article{uneatlantico17867,
         journal = {Critical Reviews in Food Science and Nutrition},
            year = {2025},
           pages = {1--15},
           month = {Noviembre},
          author = {Manuela Cassotta and Yasmany Armas Diaz and Danila Cianciosi and Bei Yang and Zexiu Qi and Ge Chen and Santos Gracia Villar and Luis Alonso Dzul L{\'o}pez and Giuseppe Grosso and Jos{\'e} L. Quiles and Jianbo Xiao and Maurizio Battino and Francesca Giampieri},
           title = {Single-cell omics for nutrition research: an emerging opportunity for human-centric investigations},
        abstract = {Understanding how dietary compounds affect human health is challenged by their molecular complexity and cell-type?specific effects. Conventional multi-cell type (bulk) analyses obscure cellular heterogeneity, while animal and standard in vitro models often fail to replicate human physiology. Single-cell omics technologies{--}such as single-cell RNA sequencing, as well as single-cell?resolved proteomic and metabolomic approaches{--}enable high-resolution investigation of nutrient?cell interactions and reveal mechanisms at a single-cell resolution. When combined with advanced human-derived in vitro systems like organoids and organ-on-chip platforms, they support mechanistic studies in physiologically relevant contexts. This review outlines emerging applications of single-cell omics in nutrition research, emphasizing their potential to uncover cell-specific dietary responses, identify nutrient-sensitive pathways, and capture interindividual variability. It also discusses key challenges{--}including technical limitations, model selection, and institutional biases{--}and identifies strategic directions to facilitate broader adoption in the field. Collectively, single-cell omics offer a transformative framework to advance human-centric nutrition research.},
        keywords = {Nutrition research; organoids; personalized nutrition; 3Rs; single-cell omics},
             url = {http://repositorio.uneatlantico.es/id/eprint/17867/}
}