eprintid: 176 rev_number: 14 eprint_status: archive userid: 2 importid: 0 dir: disk0/00/00/01/76 datestamp: 2021-05-31 14:17:30 lastmod: 2022-03-03 23:55:12 status_changed: 2021-05-31 14:17:30 type: article succeeds: 0 commentary: 0 metadata_visibility: show item_issues_count: 0 sword_depositor: 0 creators_name: Alvarez-Suarez, José Miguel creators_name: Giampieri, Francesca creators_name: Cordero, Mario creators_name: Gasparrini, Massimiliano creators_name: Forbes-Hernandez, Tamara Yuliet creators_name: Mazzoni, Luca creators_name: Afrin, Sadia creators_name: Beltrán-Ayala, Pablo creators_name: González-Paramás, Ana María creators_name: Santos-Buelga, Celestino creators_name: Varela-Lopez, Alfonso creators_name: Quiles, José Luis creators_name: Battino, Maurizio creators_id: creators_id: creators_id: creators_id: creators_id: tamara.forbes@uneatlantico.es creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: maurizio.battino@uneatlantico.es title: Activation of AMPK/Nrf2 signalling by Manuka honey protects human dermal fibroblasts against oxidative damage by improving antioxidant response and mitochondrial function promoting wound healing ispublished: pub subjects: uneat_bm divisions: uneatlantico_produccion_cientifica full_text_status: none keywords: Manuka honey, ROSAMPK, Nrf2Wound healing abstract: Excessive amounts of free radicals are deleterious for cells, resulting in cell damage, affecting the wound healing process and causing premature ageing or even neoplastic transformation. Here the capacity of Manuka honey (MH) to protect against oxidative damage and improve the process of skin wound healing was investigated. Up to 16 compounds were identified in MH, with leptosin derivatives and methyl syringate as the major ones. MH protected against apoptosis, intracellular ROS production, and lipid and protein oxidative damage. MH also protected mitochondrial functionality, promoted cell proliferation and activated the AMPK/Nrf2/ARE signalling pathway, as well as the expression of the antioxidant enzymes such as SOD and CAT. Here we describe for the first time that one of the possible mechanisms by which MH exhibits its ability to promote wound healing could be due to its capacity to improve the antioxidant response by activating AMPK phosphorylation and the ARE response. date: 2016 date_type: published publication: Journal of Functional Foods volume: 25 publisher: ELSEVIER pagerange: 38-49 pages: 0 id_number: doi:10.1016/j.jff.2016.05.008 refereed: TRUE issn: 1756-4646 official_url: http://doi.org/10.1016/j.jff.2016.05.008 num_pieces: 0 gscholar_impact: 0 gscholar_datestamp: 0000-00-00 00:00:00 access: close language: en citation: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Producción Científica Cerrado Inglés Excessive amounts of free radicals are deleterious for cells, resulting in cell damage, affecting the wound healing process and causing premature ageing or even neoplastic transformation. Here the capacity of Manuka honey (MH) to protect against oxidative damage and improve the process of skin wound healing was investigated. Up to 16 compounds were identified in MH, with leptosin derivatives and methyl syringate as the major ones. MH protected against apoptosis, intracellular ROS production, and lipid and protein oxidative damage. MH also protected mitochondrial functionality, promoted cell proliferation and activated the AMPK/Nrf2/ARE signalling pathway, as well as the expression of the antioxidant enzymes such as SOD and CAT. Here we describe for the first time that one of the possible mechanisms by which MH exhibits its ability to promote wound healing could be due to its capacity to improve the antioxidant response by activating AMPK phosphorylation and the ARE response. metadata Alvarez-Suarez, José Miguel; Giampieri, Francesca; Cordero, Mario; Gasparrini, Massimiliano; Forbes-Hernandez, Tamara Yuliet; Mazzoni, Luca; Afrin, Sadia; Beltrán-Ayala, Pablo; González-Paramás, Ana María; Santos-Buelga, Celestino; Varela-Lopez, Alfonso; Quiles, José Luis y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, tamara.forbes@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2016) Activation of AMPK/Nrf2 signalling by Manuka honey protects human dermal fibroblasts against oxidative damage by improving antioxidant response and mitochondrial function promoting wound healing. Journal of Functional Foods, 25. pp. 38-49. ISSN 1756-4646