relation: http://repositorio.uneatlantico.es/id/eprint/176/ canonical: http://repositorio.uneatlantico.es/id/eprint/176/ title: Activation of AMPK/Nrf2 signalling by Manuka honey protects human dermal fibroblasts against oxidative damage by improving antioxidant response and mitochondrial function promoting wound healing creator: Alvarez-Suarez, José Miguel creator: Giampieri, Francesca creator: Cordero, Mario creator: Gasparrini, Massimiliano creator: Forbes-Hernandez, Tamara Yuliet creator: Mazzoni, Luca creator: Afrin, Sadia creator: Beltrán-Ayala, Pablo creator: González-Paramás, Ana María creator: Santos-Buelga, Celestino creator: Varela-Lopez, Alfonso creator: Quiles, José Luis creator: Battino, Maurizio subject: Biomedicina description: Excessive amounts of free radicals are deleterious for cells, resulting in cell damage, affecting the wound healing process and causing premature ageing or even neoplastic transformation. Here the capacity of Manuka honey (MH) to protect against oxidative damage and improve the process of skin wound healing was investigated. Up to 16 compounds were identified in MH, with leptosin derivatives and methyl syringate as the major ones. MH protected against apoptosis, intracellular ROS production, and lipid and protein oxidative damage. MH also protected mitochondrial functionality, promoted cell proliferation and activated the AMPK/Nrf2/ARE signalling pathway, as well as the expression of the antioxidant enzymes such as SOD and CAT. Here we describe for the first time that one of the possible mechanisms by which MH exhibits its ability to promote wound healing could be due to its capacity to improve the antioxidant response by activating AMPK phosphorylation and the ARE response. publisher: ELSEVIER date: 2016 type: Artículo type: PeerReviewed identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Producción Científica Cerrado Inglés Excessive amounts of free radicals are deleterious for cells, resulting in cell damage, affecting the wound healing process and causing premature ageing or even neoplastic transformation. Here the capacity of Manuka honey (MH) to protect against oxidative damage and improve the process of skin wound healing was investigated. Up to 16 compounds were identified in MH, with leptosin derivatives and methyl syringate as the major ones. MH protected against apoptosis, intracellular ROS production, and lipid and protein oxidative damage. MH also protected mitochondrial functionality, promoted cell proliferation and activated the AMPK/Nrf2/ARE signalling pathway, as well as the expression of the antioxidant enzymes such as SOD and CAT. Here we describe for the first time that one of the possible mechanisms by which MH exhibits its ability to promote wound healing could be due to its capacity to improve the antioxidant response by activating AMPK phosphorylation and the ARE response. metadata Alvarez-Suarez, José Miguel; Giampieri, Francesca; Cordero, Mario; Gasparrini, Massimiliano; Forbes-Hernandez, Tamara Yuliet; Mazzoni, Luca; Afrin, Sadia; Beltrán-Ayala, Pablo; González-Paramás, Ana María; Santos-Buelga, Celestino; Varela-Lopez, Alfonso; Quiles, José Luis y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, tamara.forbes@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2016) Activation of AMPK/Nrf2 signalling by Manuka honey protects human dermal fibroblasts against oxidative damage by improving antioxidant response and mitochondrial function promoting wound healing. Journal of Functional Foods, 25. pp. 38-49. ISSN 1756-4646 relation: http://doi.org/10.1016/j.jff.2016.05.008 relation: doi:10.1016/j.jff.2016.05.008 language: en