eprintid: 17571 rev_number: 8 eprint_status: archive userid: 2 dir: disk0/00/01/75/71 datestamp: 2025-04-09 23:30:16 lastmod: 2025-04-09 23:30:16 status_changed: 2025-04-09 23:30:16 type: article metadata_visibility: show creators_name: Hu, Weizhong creators_name: Zhou, Shuxin creators_name: Ibrahim, Amel creators_name: Li, Guannan creators_name: Awad, Sameh creators_name: Ramos-Vivas, José creators_name: Kan, Jianquan creators_name: Du, Muying creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: jose.ramos@uneatlantico.es creators_id: creators_id: title: Whole Genome Analysis of Pediococcus acidilactici XJ-24 and Its Role in Preventing Listeria monocytogenes ATCC® 19115TM Infection in C57BL/6 Mice ispublished: pub subjects: uneat_bm divisions: uneatlantico_produccion_cientifica full_text_status: public keywords: probiotic; whole-genome analysis; intestinal barrier; intestinal microbiome abstract: Background/Objectives: As probiotics gain prominence in the prevention and treatment of intestinal diseases, their protective effects against pathogens and influence on host health have drawn significant attention. This study investigates the genomic characteristics and functional potential of Pediococcus acidilactici XJ-24 (XJ-24) in the prevention of Listeria monocytogenes (LM) infection in mice. Methods/Results: Whole-genome analysis confirmed the safety and probiotic properties of XJ-24, including acid and bile salt tolerance, antimicrobial activity, and safety. In vivo, C57BL/6 mice challenges indicated that XJ-24 significantly reduced LM colonization, suppressed pro-inflammatory cytokines (IL-1β, IL-6, TNF-α, IFN-γ), alleviated colon and spleen tissue damage, and maintained intestinal barrier integrity by upregulating tight junction proteins (Occludin, Claudin-1, ZO-1). Moreover, XJ-24 modulated gut microbiota composition by increasing beneficial taxa while reducing harmful bacteria. Correlation analysis highlighted a positive association between Lachnospiraceae and tight junction proteins. Conclusions: These findings demonstrate the potential of XJ-24 as a functional probiotic for preventing LM infection and provide a basis for further clinical exploration. date: 2025-03 publication: Antibiotics volume: 14 number: 3 pagerange: 323 id_number: doi:10.3390/antibiotics14030323 refereed: TRUE issn: 2079-6382 official_url: http://doi.org/10.3390/antibiotics14030323 access: open language: en citation: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Artículos y libros Abierto Inglés Background/Objectives: As probiotics gain prominence in the prevention and treatment of intestinal diseases, their protective effects against pathogens and influence on host health have drawn significant attention. This study investigates the genomic characteristics and functional potential of Pediococcus acidilactici XJ-24 (XJ-24) in the prevention of Listeria monocytogenes (LM) infection in mice. Methods/Results: Whole-genome analysis confirmed the safety and probiotic properties of XJ-24, including acid and bile salt tolerance, antimicrobial activity, and safety. In vivo, C57BL/6 mice challenges indicated that XJ-24 significantly reduced LM colonization, suppressed pro-inflammatory cytokines (IL-1β, IL-6, TNF-α, IFN-γ), alleviated colon and spleen tissue damage, and maintained intestinal barrier integrity by upregulating tight junction proteins (Occludin, Claudin-1, ZO-1). Moreover, XJ-24 modulated gut microbiota composition by increasing beneficial taxa while reducing harmful bacteria. Correlation analysis highlighted a positive association between Lachnospiraceae and tight junction proteins. Conclusions: These findings demonstrate the potential of XJ-24 as a functional probiotic for preventing LM infection and provide a basis for further clinical exploration. metadata Hu, Weizhong; Zhou, Shuxin; Ibrahim, Amel; Li, Guannan; Awad, Sameh; Ramos-Vivas, José; Kan, Jianquan y Du, Muying mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, jose.ramos@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR (2025) Whole Genome Analysis of Pediococcus acidilactici XJ-24 and Its Role in Preventing Listeria monocytogenes ATCC® 19115TM Infection in C57BL/6 Mice. Antibiotics, 14 (3). p. 323. ISSN 2079-6382 document_url: http://repositorio.uneatlantico.es/id/eprint/17571/1/antibiotics-14-00323.pdf