eprintid: 142 rev_number: 8 eprint_status: archive userid: 2 importid: 0 dir: disk0/00/00/01/42 datestamp: 2021-06-02 23:55:08 lastmod: 2022-03-03 23:55:09 status_changed: 2021-06-02 23:55:08 type: article succeeds: 0 commentary: 0 metadata_visibility: show item_issues_count: 0 sword_depositor: 0 creators_name: Gasparrini, Massimiliano creators_name: Forbes-Hernandez, Tamara Y. creators_name: Giampieri, Francesca creators_name: Afrin, Sadia creators_name: Alvarez-Suarez, Josè M. creators_name: Mazzoni, Luca creators_name: Mezzetti, Bruno creators_name: Quiles, Josè L. creators_name: Battino, Maurizio creators_id: creators_id: tamara.forbes@unini.edu.mx creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: maurizio.battino@uneatlantico.es title: Anti-inflammatory effect of strawberry extract against LPS-induced stress in RAW 264.7 macrophages ispublished: pub subjects: uneat_bm divisions: uneatlantico_produccion_cientifica divisions: uninimx_produccion_cientifica full_text_status: none keywords: Strawberry; Phenolic compounds; LPS; Inflammation; ROS; Prevention. abstract: A common denominator in the pathogenesis of most chronic inflammatory diseases is the involvement of oxidative stress, related to ROS production by all aerobic organisms. Dietary antioxidants from plant foods represent an efficient strategy to counteract this condition. The aim of the present study was to evaluate the protective effects of strawberry extracts on inflammatory status induced by E. Coli LPS on RAW 264.7 macrophages by measuring the main oxidative and inflammatory biomarkers and investigating the molecular pathways involved. Strawberry pre-treatment efficiently counteracted LPS-induced oxidative stress reducing the amount of ROS and nitrite production, stimulating endogenous antioxidant enzyme activities and enhancing protection against lipid, protein and DNA damage (P < 0.05). Strawberry pre-treatment exerted these protective effects primarily through the activation of the Nrf2 pathway, which is markedly AMPK-dependent and also by the modulation of the NF-kB signalling pathway. Finally, an improvement in mitochondria functionality was also detected. The results obtained in this work highlight the health benefit of strawberries against inflammatory and oxidative stress in LPS-stimulated RAW 264.7 macrophages, investigating for the first time the possible involved molecular mechanisms. date: 2017 date_type: published publication: Food and Chemical Toxicology volume: 102 pagerange: 1-10 pages: 0 id_number: doi:10.1016/j.fct.2017.01.018 refereed: TRUE issn: 0278-6915 official_url: http://doi.org/10.1016/j.fct.2017.01.018 num_pieces: 0 gscholar_impact: 0 gscholar_datestamp: 0000-00-00 00:00:00 access: close language: en citation: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Producción Científica Universidad Internacional Iberoamericana México > Investigación > Producción Científica Cerrado Inglés A common denominator in the pathogenesis of most chronic inflammatory diseases is the involvement of oxidative stress, related to ROS production by all aerobic organisms. Dietary antioxidants from plant foods represent an efficient strategy to counteract this condition. The aim of the present study was to evaluate the protective effects of strawberry extracts on inflammatory status induced by E. Coli LPS on RAW 264.7 macrophages by measuring the main oxidative and inflammatory biomarkers and investigating the molecular pathways involved. Strawberry pre-treatment efficiently counteracted LPS-induced oxidative stress reducing the amount of ROS and nitrite production, stimulating endogenous antioxidant enzyme activities and enhancing protection against lipid, protein and DNA damage (P < 0.05). Strawberry pre-treatment exerted these protective effects primarily through the activation of the Nrf2 pathway, which is markedly AMPK-dependent and also by the modulation of the NF-kB signalling pathway. Finally, an improvement in mitochondria functionality was also detected. The results obtained in this work highlight the health benefit of strawberries against inflammatory and oxidative stress in LPS-stimulated RAW 264.7 macrophages, investigating for the first time the possible involved molecular mechanisms. metadata Gasparrini, Massimiliano; Forbes-Hernandez, Tamara Y.; Giampieri, Francesca; Afrin, Sadia; Alvarez-Suarez, Josè M.; Mazzoni, Luca; Mezzetti, Bruno; Quiles, Josè L. y Battino, Maurizio mail SIN ESPECIFICAR, tamara.forbes@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2017) Anti-inflammatory effect of strawberry extract against LPS-induced stress in RAW 264.7 macrophages. Food and Chemical Toxicology, 102. pp. 1-10. ISSN 0278-6915