eprintid: 130 rev_number: 13 eprint_status: archive userid: 2 importid: 0 dir: disk0/00/00/01/30 datestamp: 2021-06-01 23:55:11 lastmod: 2021-07-08 23:55:14 status_changed: 2021-06-01 23:55:11 type: article succeeds: 0 commentary: 0 metadata_visibility: show item_issues_count: 0 sword_depositor: 0 creators_name: Giampieri, Francesca creators_name: Afrin, Sadia creators_name: Stewart, Derek creators_name: McDougall, Gordon creators_name: Brennan, Rex creators_name: Blyth, Lesley creators_name: Gasparrini, Massimiliano creators_name: Mazzoni, Luca creators_name: Capocasa, Franco creators_name: Alvarez-Suarez, José M. creators_name: Bompadre, Stefano creators_name: Nogueira Brás de Oliveira, Pedro creators_name: N. Santos, Claudia creators_name: Masias Vergara, Manuel creators_name: Agudo Toyos, Pablo creators_name: Crespo-Álvarez, Jorge creators_name: Mezzetti, Bruno creators_name: Forbes-Hernandez, Tamara Y. creators_name: Battino, Maurizio creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: manuel.masias@uneatlantico.es creators_id: pablo.agudo@uneatlantico.es creators_id: jorge.crespo@uneatlantico.es creators_id: creators_id: creators_id: title: Phytochemical Composition and Cytotoxic Effects on Liver Hepatocellular Carcinoma Cells of Different Berries Following a Simulated In Vitro Gastrointestinal Digestion ispublished: pub subjects: uneat_bm divisions: uneatlantico_produccion_cientifica full_text_status: public keywords: Berry; Polyphenols; In vitro gastrointestinal digestion; Bioavailability; Cytotoxicity. abstract: Berry fruits are rich in nutrients and polyphenols, providing potential health benefits. Understanding the factors that affect their bioavailability is becoming of utmost importance for evaluating their biological significance and efficacy as functional food. In this study, the phytochemical composition and the total antioxidant capacity of different varieties of five berries (blackberry, blackcurrant, blueberry, raspberry, and strawberry) were evaluated after an in vitro gastrointestinal digestion process. The cultivar of each berry that showed the higher content of total phenols and flavonoids was selected to study its cytotoxic effect on human hepatoma cells. Digestion resulted in a high reduction (p ˂ 0.05) of total phenolic, flavonoid and anthocyanin contents and total antioxidant capacity, in the “IN” samples compared to the “OUT” extracts, which represent the “serum-available” and the “colon-available” fractions, respectively. Incubation of the digested fraction for 24 h didn’t exert any effect on cellular viability, while a dose- and time-dependent cytotoxicity was observed after 48 h and 72 h of incubation for all the berries analyzed. Our results suggest that the approach proposed in this work may represent a rapid tool for evaluating and identifying new berries with increased phytochemical bioavailability, highlighting their antiproliferative agents after an in vitro digestion. date: 2018 date_type: published publication: Molecules volume: 23 number: 8 pagerange: 1918 pages: 0 id_number: doi:10.3390/molecules23081918 refereed: TRUE issn: 1420-3049 official_url: http://doi.org/10.3390/molecules23081918 num_pieces: 0 gscholar_impact: 0 gscholar_datestamp: 0000-00-00 00:00:00 citation: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Producción Científica SIN ESPECIFICAR SIN ESPECIFICAR Berry fruits are rich in nutrients and polyphenols, providing potential health benefits. Understanding the factors that affect their bioavailability is becoming of utmost importance for evaluating their biological significance and efficacy as functional food. In this study, the phytochemical composition and the total antioxidant capacity of different varieties of five berries (blackberry, blackcurrant, blueberry, raspberry, and strawberry) were evaluated after an in vitro gastrointestinal digestion process. The cultivar of each berry that showed the higher content of total phenols and flavonoids was selected to study its cytotoxic effect on human hepatoma cells. Digestion resulted in a high reduction (p ˂ 0.05) of total phenolic, flavonoid and anthocyanin contents and total antioxidant capacity, in the “IN” samples compared to the “OUT” extracts, which represent the “serum-available” and the “colon-available” fractions, respectively. Incubation of the digested fraction for 24 h didn’t exert any effect on cellular viability, while a dose- and time-dependent cytotoxicity was observed after 48 h and 72 h of incubation for all the berries analyzed. Our results suggest that the approach proposed in this work may represent a rapid tool for evaluating and identifying new berries with increased phytochemical bioavailability, highlighting their antiproliferative agents after an in vitro digestion. metadata Giampieri, Francesca; Afrin, Sadia; Stewart, Derek; McDougall, Gordon; Brennan, Rex; Blyth, Lesley; Gasparrini, Massimiliano; Mazzoni, Luca; Capocasa, Franco; Alvarez-Suarez, José M.; Bompadre, Stefano; Nogueira Brás de Oliveira, Pedro; N. Santos, Claudia; Masias Vergara, Manuel; Agudo Toyos, Pablo; Crespo-Álvarez, Jorge; Mezzetti, Bruno; Forbes-Hernandez, Tamara Y. y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, manuel.masias@uneatlantico.es, pablo.agudo@uneatlantico.es, jorge.crespo@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2018) Phytochemical Composition and Cytotoxic Effects on Liver Hepatocellular Carcinoma Cells of Different Berries Following a Simulated In Vitro Gastrointestinal Digestion. Molecules, 23 (8). p. 1918. ISSN 1420-3049 document_url: http://repositorio.uneatlantico.es/id/eprint/130/1/molecules-23-01918.pdf