TY  - JOUR
VL  - 23
SN  - 1420-3049
UR  - http://doi.org/10.3390/molecules23081918
JF  - Molecules
N2  - Berry fruits are rich in nutrients and polyphenols, providing potential health benefits. Understanding the factors that affect their bioavailability is becoming of utmost importance for evaluating their biological significance and efficacy as functional food. In this study, the phytochemical composition and the total antioxidant capacity of different varieties of five berries (blackberry, blackcurrant, blueberry, raspberry, and strawberry) were evaluated after an in vitro gastrointestinal digestion process. The cultivar of each berry that showed the higher content of total phenols and flavonoids was selected to study its cytotoxic effect on human hepatoma cells. Digestion resulted in a high reduction (p ? 0.05) of total phenolic, flavonoid and anthocyanin contents and total antioxidant capacity, in the ?IN? samples compared to the ?OUT? extracts, which represent the ?serum-available? and the ?colon-available? fractions, respectively. Incubation of the digested fraction for 24 h didn?t exert any effect on cellular viability, while a dose- and time-dependent cytotoxicity was observed after 48 h and 72 h of incubation for all the berries analyzed. Our results suggest that the approach proposed in this work may represent a rapid tool for evaluating and identifying new berries with increased phytochemical bioavailability, highlighting their antiproliferative agents after an in vitro digestion.
Y1  - 2018///
IS  - 8
A1  - Giampieri, Francesca
A1  - Afrin, Sadia
A1  - Stewart, Derek
A1  - McDougall, Gordon
A1  - Brennan, Rex
A1  - Blyth, Lesley
A1  - Gasparrini, Massimiliano
A1  - Mazzoni, Luca
A1  - Capocasa, Franco
A1  - Alvarez-Suarez, José M.
A1  - Bompadre, Stefano
A1  - Nogueira Brás de Oliveira, Pedro
A1  - N. Santos, Claudia
A1  - Masias Vergara, Manuel
A1  - Agudo Toyos, Pablo
A1  - Crespo-Álvarez, Jorge
A1  - Mezzetti, Bruno
A1  - Forbes-Hernandez, Tamara Y.
A1  - Battino, Maurizio
AV  - public
KW  - Berry; Polyphenols; In vitro gastrointestinal digestion; Bioavailability; Cytotoxicity.
ID  - uneatlantico130
TI  - Phytochemical Composition and Cytotoxic Effects on Liver Hepatocellular Carcinoma Cells of Different Berries Following a Simulated In Vitro Gastrointestinal Digestion
ER  -