relation: http://repositorio.uneatlantico.es/id/eprint/130/ canonical: http://repositorio.uneatlantico.es/id/eprint/130/ title: Phytochemical Composition and Cytotoxic Effects on Liver Hepatocellular Carcinoma Cells of Different Berries Following a Simulated In Vitro Gastrointestinal Digestion creator: Giampieri, Francesca creator: Afrin, Sadia creator: Stewart, Derek creator: McDougall, Gordon creator: Brennan, Rex creator: Blyth, Lesley creator: Gasparrini, Massimiliano creator: Mazzoni, Luca creator: Capocasa, Franco creator: Alvarez-Suarez, José M. creator: Bompadre, Stefano creator: Nogueira Brás de Oliveira, Pedro creator: N. Santos, Claudia creator: Masias Vergara, Manuel creator: Agudo Toyos, Pablo creator: Crespo-Álvarez, Jorge creator: Mezzetti, Bruno creator: Forbes-Hernandez, Tamara Y. creator: Battino, Maurizio subject: Biomedicina description: Berry fruits are rich in nutrients and polyphenols, providing potential health benefits. Understanding the factors that affect their bioavailability is becoming of utmost importance for evaluating their biological significance and efficacy as functional food. In this study, the phytochemical composition and the total antioxidant capacity of different varieties of five berries (blackberry, blackcurrant, blueberry, raspberry, and strawberry) were evaluated after an in vitro gastrointestinal digestion process. The cultivar of each berry that showed the higher content of total phenols and flavonoids was selected to study its cytotoxic effect on human hepatoma cells. Digestion resulted in a high reduction (p ˂ 0.05) of total phenolic, flavonoid and anthocyanin contents and total antioxidant capacity, in the “IN” samples compared to the “OUT” extracts, which represent the “serum-available” and the “colon-available” fractions, respectively. Incubation of the digested fraction for 24 h didn’t exert any effect on cellular viability, while a dose- and time-dependent cytotoxicity was observed after 48 h and 72 h of incubation for all the berries analyzed. Our results suggest that the approach proposed in this work may represent a rapid tool for evaluating and identifying new berries with increased phytochemical bioavailability, highlighting their antiproliferative agents after an in vitro digestion. date: 2018 type: Artículo type: PeerReviewed format: text language: en rights: cc_by_4 identifier: http://repositorio.uneatlantico.es/id/eprint/130/1/molecules-23-01918.pdf identifier: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Producción Científica SIN ESPECIFICAR SIN ESPECIFICAR Berry fruits are rich in nutrients and polyphenols, providing potential health benefits. Understanding the factors that affect their bioavailability is becoming of utmost importance for evaluating their biological significance and efficacy as functional food. In this study, the phytochemical composition and the total antioxidant capacity of different varieties of five berries (blackberry, blackcurrant, blueberry, raspberry, and strawberry) were evaluated after an in vitro gastrointestinal digestion process. The cultivar of each berry that showed the higher content of total phenols and flavonoids was selected to study its cytotoxic effect on human hepatoma cells. Digestion resulted in a high reduction (p ˂ 0.05) of total phenolic, flavonoid and anthocyanin contents and total antioxidant capacity, in the “IN” samples compared to the “OUT” extracts, which represent the “serum-available” and the “colon-available” fractions, respectively. Incubation of the digested fraction for 24 h didn’t exert any effect on cellular viability, while a dose- and time-dependent cytotoxicity was observed after 48 h and 72 h of incubation for all the berries analyzed. Our results suggest that the approach proposed in this work may represent a rapid tool for evaluating and identifying new berries with increased phytochemical bioavailability, highlighting their antiproliferative agents after an in vitro digestion. metadata Giampieri, Francesca; Afrin, Sadia; Stewart, Derek; McDougall, Gordon; Brennan, Rex; Blyth, Lesley; Gasparrini, Massimiliano; Mazzoni, Luca; Capocasa, Franco; Alvarez-Suarez, José M.; Bompadre, Stefano; Nogueira Brás de Oliveira, Pedro; N. Santos, Claudia; Masias Vergara, Manuel; Agudo Toyos, Pablo; Crespo-Álvarez, Jorge; Mezzetti, Bruno; Forbes-Hernandez, Tamara Y. y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, manuel.masias@uneatlantico.es, pablo.agudo@uneatlantico.es, jorge.crespo@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR (2018) Phytochemical Composition and Cytotoxic Effects on Liver Hepatocellular Carcinoma Cells of Different Berries Following a Simulated In Vitro Gastrointestinal Digestion. Molecules, 23 (8). p. 1918. ISSN 1420-3049 relation: http://doi.org/10.3390/molecules23081918 relation: doi:10.3390/molecules23081918