eprintid: 129 rev_number: 8 eprint_status: archive userid: 2 importid: 0 dir: disk0/00/00/01/29 datestamp: 2021-06-01 23:55:11 lastmod: 2022-03-03 23:55:09 status_changed: 2021-06-01 23:55:11 type: article succeeds: 0 commentary: 0 metadata_visibility: show item_issues_count: 0 sword_depositor: 0 creators_name: Gasparrini, Massimiliano creators_name: Giampieri, Francesca creators_name: Forbes-Hernandez, Tamara Y. creators_name: Afrin, Sadia creators_name: Cianciosi, Danila creators_name: Reboredo-Rodriguez, Patricia creators_name: Varela-Lopez, Alfonso creators_name: Zhang, JiaoJiao creators_name: Quiles, Josè L. creators_name: Mezzetti, Bruno creators_name: Bompadre, Stefano creators_name: Battino, Maurizio creators_id: creators_id: creators_id: tamara.forbes@unini.edu.mx creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: maurizio.battino@uneatlantico.es title: Strawberry extracts efficiently counteract inflammatory stress induced by the endotoxin lipopolysaccharide in Human Dermal Fibroblast ispublished: pub subjects: uneat_bm divisions: uneatlantico_produccion_cientifica divisions: uninimx_produccion_cientifica full_text_status: none keywords: Strawberry; LPS; Human Dermal Fibroblast; Inflammation; Oxidative stress; Antioxidant response; Prevention. abstract: A protracted pro-inflammatory state is the common denominator in the development, progression and complication of the common chronic diseases. Dietary antioxidants represent an efficient tool to counteract this inflammatory state. The aim of the present work was to evaluate the effects of strawberry extracts on inflammation evoked by E. Coli lipopolysaccharide in Human Dermal Fibroblast, by measuring reactive oxygen species production, apoptosis rate, antioxidant enzymes activity, mitochondria functionality and also investigating the molecular pathway involved in inflammatory and antioxidant response. The results demonstrated that strawberry pre-treatment reduced intracellular reactive oxygen species levels, apoptotic rate, improved antioxidant defences and mitochondria functionality in lipopolysaccharide -treated cells. Strawberry exerted these protective activities through the inhibition of the NF-kB signalling pathway and the stimulation of the Nrf2 pathway, with a mechanism AMPK-dependent. These results confirm the health benefits of strawberry in the prevention of inflammation and oxidative stress condition in lipopolysaccharide-treated cells. date: 2018 date_type: published publication: Food and Chemical Toxicology volume: 114 pagerange: 128-140 pages: 0 id_number: doi:10.1016/j.fct.2018.02.038 refereed: TRUE issn: 0278-6915 official_url: http://doi.org/10.1016/j.fct.2018.02.038 num_pieces: 0 gscholar_impact: 0 gscholar_datestamp: 0000-00-00 00:00:00 access: close language: en citation: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Producción Científica Universidad Internacional Iberoamericana México > Investigación > Producción Científica Cerrado Inglés A protracted pro-inflammatory state is the common denominator in the development, progression and complication of the common chronic diseases. Dietary antioxidants represent an efficient tool to counteract this inflammatory state. The aim of the present work was to evaluate the effects of strawberry extracts on inflammation evoked by E. Coli lipopolysaccharide in Human Dermal Fibroblast, by measuring reactive oxygen species production, apoptosis rate, antioxidant enzymes activity, mitochondria functionality and also investigating the molecular pathway involved in inflammatory and antioxidant response. The results demonstrated that strawberry pre-treatment reduced intracellular reactive oxygen species levels, apoptotic rate, improved antioxidant defences and mitochondria functionality in lipopolysaccharide -treated cells. Strawberry exerted these protective activities through the inhibition of the NF-kB signalling pathway and the stimulation of the Nrf2 pathway, with a mechanism AMPK-dependent. These results confirm the health benefits of strawberry in the prevention of inflammation and oxidative stress condition in lipopolysaccharide-treated cells. metadata Gasparrini, Massimiliano; Giampieri, Francesca; Forbes-Hernandez, Tamara Y.; Afrin, Sadia; Cianciosi, Danila; Reboredo-Rodriguez, Patricia; Varela-Lopez, Alfonso; Zhang, JiaoJiao; Quiles, Josè L.; Mezzetti, Bruno; Bompadre, Stefano y Battino, Maurizio mail SIN ESPECIFICAR, SIN ESPECIFICAR, tamara.forbes@unini.edu.mx, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, maurizio.battino@uneatlantico.es (2018) Strawberry extracts efficiently counteract inflammatory stress induced by the endotoxin lipopolysaccharide in Human Dermal Fibroblast. Food and Chemical Toxicology, 114. pp. 128-140. ISSN 0278-6915