eprintid: 123 rev_number: 10 eprint_status: archive userid: 2 importid: 0 dir: disk0/00/00/01/23 datestamp: 2021-06-01 23:55:10 lastmod: 2023-06-30 23:30:18 status_changed: 2021-06-01 23:55:10 type: article succeeds: 0 commentary: 0 metadata_visibility: show item_issues_count: 0 sword_depositor: 0 creators_name: Navarro-Pando, José M. creators_name: Bullón, Pedro creators_name: Cordero, Mario D. creators_name: Alcocer-Gómez, Elísabet creators_id: jose.navarro@uneatlantico.es creators_id: creators_id: mario.cordero@uneatlantico.es creators_id: title: Is AMP-Activated Protein Kinase Associated to the Metabolic Changes in Primary Ovarian Insufficiency? ispublished: pub subjects: uneat_bm divisions: uneatlantico_produccion_cientifica full_text_status: none keywords: Primary ovarian insufficiency; AMPK; Inflammation; Granulosa cells. abstract: Primary ovarian insufficiency (POI) is a critical fertility defect characterized by anticipated impairment of the follicular reserve, which pathophysiological mechanisms have not yet been identified. We have investigated the possible relationship between AMP-activated protein kinase (AMPK) and the pathophysiology of POI. We studied 35 POI patients with altered levels of follicle-stimulating hormone (FSH) and estradiol and increased percentage of overweight compared with 20 healthy women. Blood mononuclear cells from POI patients showed reduced levels of phosphorylated AMPK, adenosine triphosphate, and high serum levels of leptin and tumor necrosis factor-alpha. All these alterations were also observed in granulosa cells, a more closer environment of the ovary. Significant negative correlations between AMPK and body mass index, FSH and leptin levels, and a positive correlation between AMPK and estradiol levels were observed. Oral metformin supplementation restored biochemical parameters. Interestingly, AMPK levels from patients were negatively correlated with age, suggesting an accelerated aging in POI mediated by the impairment of AMPK. These results lead to the hypothesis that an impairment of AMPK could be implicated in the metabolic pathophysiology of POI indicating at AMPK induction as a possible new therapeutic target. date: 2020-10 date_type: published publication: Antioxidants & Redox Signaling volume: 33 number: 15 pagerange: 1115-1121 pages: 0 id_number: doi:10.1089/ars.2020.8144 refereed: TRUE issn: 1523-0864 official_url: http://doi.org/10.1089/ars.2020.8144 num_pieces: 0 gscholar_impact: 0 gscholar_datestamp: 0000-00-00 00:00:00 access: close language: en citation: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Producción Científica Cerrado Inglés Primary ovarian insufficiency (POI) is a critical fertility defect characterized by anticipated impairment of the follicular reserve, which pathophysiological mechanisms have not yet been identified. We have investigated the possible relationship between AMP-activated protein kinase (AMPK) and the pathophysiology of POI. We studied 35 POI patients with altered levels of follicle-stimulating hormone (FSH) and estradiol and increased percentage of overweight compared with 20 healthy women. Blood mononuclear cells from POI patients showed reduced levels of phosphorylated AMPK, adenosine triphosphate, and high serum levels of leptin and tumor necrosis factor-alpha. All these alterations were also observed in granulosa cells, a more closer environment of the ovary. Significant negative correlations between AMPK and body mass index, FSH and leptin levels, and a positive correlation between AMPK and estradiol levels were observed. Oral metformin supplementation restored biochemical parameters. Interestingly, AMPK levels from patients were negatively correlated with age, suggesting an accelerated aging in POI mediated by the impairment of AMPK. These results lead to the hypothesis that an impairment of AMPK could be implicated in the metabolic pathophysiology of POI indicating at AMPK induction as a possible new therapeutic target. metadata Navarro-Pando, José M.; Bullón, Pedro; Cordero, Mario D. y Alcocer-Gómez, Elísabet mail jose.navarro@uneatlantico.es, SIN ESPECIFICAR, mario.cordero@uneatlantico.es, SIN ESPECIFICAR (2020) Is AMP-Activated Protein Kinase Associated to the Metabolic Changes in Primary Ovarian Insufficiency? Antioxidants & Redox Signaling, 33 (15). pp. 1115-1121. ISSN 1523-0864