eprintid: 119 rev_number: 11 eprint_status: archive userid: 2 importid: 0 dir: disk0/00/00/01/19 datestamp: 2021-06-01 23:55:08 lastmod: 2023-07-03 23:30:16 status_changed: 2021-06-01 23:55:08 type: article succeeds: 0 commentary: 0 metadata_visibility: show item_issues_count: 0 sword_depositor: 0 creators_name: Garnacho-Montero, José creators_name: Palacios-García, Inmaculada creators_name: Díaz-Martín, Ana creators_name: Gutiérrez-Pizarraya, Antonio creators_name: López-Sánchez, José M. creators_name: Gómez, Elísabet Alcocer creators_name: Cordero, Mario D. creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: mario.cordero@uneatlantico.es title: Sequential Changes of NLRP3 Inflammasome Activation in Sepsis and its Relationship With Death ispublished: pub subjects: uneat_bm divisions: uneatlantico_produccion_cientifica full_text_status: none keywords: Caspase-1; Caspase-3; inflammation ; Mortality; NLRP3 inflammasome NLRP3 inflammasome; Sepsis. abstract: Introduction: Inflammasomes are recognized as key components of the innate immune response in sepsis. We aimed to describe the transcriptional expression of nucleotide-binding domain, leucine-rich repeat–containing receptor, pyrin domain–containing-3 (NLRP3), and serum interleukin-1β (IL-1 β) in critically ill patients, their changes over the first week and their prognostic value in septic patients. Methods: Prospective study including patients with sepsis based on Sepsis-3 definitions and a control group of critically ill patients without sepsis. We measured the circulating levels of IL-1β as well as the transcriptional expression of NLRP3 at admission and on days 3 and 7. Caspase-1 and caspase-3 activation was analyzed in a matched cohort of patients with septic shock (four dead and four survivors). Results: Fifty-five septic patients and 11 non-septic patients were studied. Levels on day 0 and 3 of IL-1 β and NLRP3 inflammasome expression were significantly higher in patients with sepsis than in controls. NLRP3 was significantly higher in septic patients who survived at day 7 without significant difference between survivors and non-survivors at baseline and on day 3. In survivors, an increased caspase-1 protein expression with reduced expression caspase-3 was observed with the opposite pattern in those who died. Conclusions: NLRP3 is activated in critically ill patients but this up-regulation is more intense in patients with sepsis. In sepsis, a sustained NLRP3 activation during the first week is protective and sepsis. An increased caspase-1 protein expression with reduced expression caspase-3 is the pattern observed in septic shock patients who survive. date: 2020-09 date_type: published publication: Shock volume: 54 number: 3 pagerange: 294-300 pages: 0 id_number: doi:10.1097/SHK.0000000000001521 refereed: TRUE issn: 1073-2322 official_url: http://doi.org/10.1097/SHK.0000000000001521 num_pieces: 0 gscholar_impact: 0 gscholar_datestamp: 0000-00-00 00:00:00 access: open language: en citation: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Producción Científica Abierto Inglés Introduction: Inflammasomes are recognized as key components of the innate immune response in sepsis. We aimed to describe the transcriptional expression of nucleotide-binding domain, leucine-rich repeat–containing receptor, pyrin domain–containing-3 (NLRP3), and serum interleukin-1β (IL-1 β) in critically ill patients, their changes over the first week and their prognostic value in septic patients. Methods: Prospective study including patients with sepsis based on Sepsis-3 definitions and a control group of critically ill patients without sepsis. We measured the circulating levels of IL-1β as well as the transcriptional expression of NLRP3 at admission and on days 3 and 7. Caspase-1 and caspase-3 activation was analyzed in a matched cohort of patients with septic shock (four dead and four survivors). Results: Fifty-five septic patients and 11 non-septic patients were studied. Levels on day 0 and 3 of IL-1 β and NLRP3 inflammasome expression were significantly higher in patients with sepsis than in controls. NLRP3 was significantly higher in septic patients who survived at day 7 without significant difference between survivors and non-survivors at baseline and on day 3. In survivors, an increased caspase-1 protein expression with reduced expression caspase-3 was observed with the opposite pattern in those who died. Conclusions: NLRP3 is activated in critically ill patients but this up-regulation is more intense in patients with sepsis. In sepsis, a sustained NLRP3 activation during the first week is protective and sepsis. An increased caspase-1 protein expression with reduced expression caspase-3 is the pattern observed in septic shock patients who survive. metadata Garnacho-Montero, José; Palacios-García, Inmaculada; Díaz-Martín, Ana; Gutiérrez-Pizarraya, Antonio; López-Sánchez, José M.; Gómez, Elísabet Alcocer y Cordero, Mario D. mail SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, mario.cordero@uneatlantico.es (2020) Sequential Changes of NLRP3 Inflammasome Activation in Sepsis and its Relationship With Death. Shock, 54 (3). pp. 294-300. ISSN 1073-2322