TY  - JOUR
IS  - 3
SP  - 294
N2  - Introduction: 
Inflammasomes are recognized as key components of the innate immune response in sepsis. We aimed to describe the transcriptional expression of nucleotide-binding domain, leucine-rich repeat?containing receptor, pyrin domain?containing-3 (NLRP3), and serum interleukin-1? (IL-1 ?) in critically ill patients, their changes over the first week and their prognostic value in septic patients.

Methods: 
Prospective study including patients with sepsis based on Sepsis-3 definitions and a control group of critically ill patients without sepsis. We measured the circulating levels of IL-1? as well as the transcriptional expression of NLRP3 at admission and on days 3 and 7. Caspase-1 and caspase-3 activation was analyzed in a matched cohort of patients with septic shock (four dead and four survivors).

Results: 
Fifty-five septic patients and 11 non-septic patients were studied. Levels on day 0 and 3 of IL-1 ? and NLRP3 inflammasome expression were significantly higher in patients with sepsis than in controls. NLRP3 was significantly higher in septic patients who survived at day 7 without significant difference between survivors and non-survivors at baseline and on day 3. In survivors, an increased caspase-1 protein expression with reduced expression caspase-3 was observed with the opposite pattern in those who died.

Conclusions: 
NLRP3 is activated in critically ill patients but this up-regulation is more intense in patients with sepsis. In sepsis, a sustained NLRP3 activation during the first week is protective and sepsis. An increased caspase-1 protein expression with reduced expression caspase-3 is the pattern observed in septic shock patients who survive.
JF  - Shock
Y1  - 2020/09//
UR  - http://doi.org/10.1097/SHK.0000000000001521
SN  - 1073-2322
VL  - 54
ID  - uneatlantico119
EP  - 300
TI  - Sequential Changes of NLRP3 Inflammasome Activation in Sepsis and its Relationship With Death
KW  - Caspase-1;  Caspase-3;  inflammation ; Mortality; NLRP3 inflammasome NLRP3 inflammasome;  Sepsis.
A1  - Garnacho-Montero, José
A1  - Palacios-García, Inmaculada
A1  - Díaz-Martín, Ana
A1  - Gutiérrez-Pizarraya, Antonio
A1  - López-Sánchez, José M.
A1  - Gómez, Elísabet Alcocer
A1  - Cordero, Mario D.
AV  - none
ER  -