eprintid: 107 rev_number: 17 eprint_status: archive userid: 2 importid: 0 dir: disk0/00/00/01/07 datestamp: 2021-06-01 23:55:05 lastmod: 2023-07-05 23:30:13 status_changed: 2021-06-01 23:55:05 type: article succeeds: 0 commentary: 0 metadata_visibility: show item_issues_count: 0 sword_depositor: 0 creators_name: Navarro-Pando, José Manuel creators_name: Alcocer-Gómez, Elísabet creators_name: Castejón-Vega, Beatriz creators_name: Navarro-Villarán, Elena creators_name: Condés-Hervás, Mónica creators_name: Mundi-Roldan, María creators_name: Muntané, Jordi creators_name: Pérez-Pulido, Antonio J. creators_name: Bullon, Pedro creators_name: Wang, Chun creators_name: Hoffman, Hal M. creators_name: Sanz, Alberto creators_name: Mbalaviele, Gabriel creators_name: Ryffel, Bernhard creators_name: Cordero, Mario D. creators_id: jose.navarro@uneatlantico.es creators_id: creators_id: creators_id: creators_id: monica.condes@uneatlantico.es creators_id: maria.mundi@uneatlantico.es creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: creators_id: mario.cordero@uneatlantico.es title: Inhibition of the NLRP3 inflammasome prevents ovarian aging ispublished: pub subjects: uneat_bm divisions: uneatlantico_produccion_cientifica full_text_status: public abstract: Inflammation is a hallmark of aging and is negatively affecting female fertility. In this study, we evaluate the role of the NLRP3 inflammasome in ovarian aging and female fertility. Age-dependent increased expression of NLRP3 in the ovary was observed in WT mice during reproductive aging. High expression of NLRP3, caspase-1, and IL-1β was also observed in granulosa cells from patients with ovarian insufficiency. Ablation of NLRP3 improved the survival and pregnancy rates and increased anti-Müllerian hormone levels and autophagy rates in ovaries. Deficiency of NLRP3 also reduced serum FSH and estradiol levels. Consistent with these results, pharmacological inhibition of NLRP3 using a direct NLRP3 inhibitor, MCC950, improved fertility in female mice to levels comparable to those of Nlrp3−/− mice. These results suggest that the NLRP3 inflammasome is implicated in the age-dependent loss of female fertility and position this inflammasome as a potential new therapeutic target for the treatment of infertility. date: 2021-01 date_type: published publication: Science Advances volume: 7 number: 1 pagerange: eabc7409 pages: 0 id_number: doi:10.1126/sciadv.abc7409 refereed: TRUE issn: 2375-2548 official_url: http://doi.org/10.1126/sciadv.abc7409 num_pieces: 0 gscholar_impact: 0 gscholar_datestamp: 0000-00-00 00:00:00 access: open language: en citation: Artículo Materias > Biomedicina Universidad Europea del Atlántico > Investigación > Producción Científica Abierto Inglés Inflammation is a hallmark of aging and is negatively affecting female fertility. In this study, we evaluate the role of the NLRP3 inflammasome in ovarian aging and female fertility. Age-dependent increased expression of NLRP3 in the ovary was observed in WT mice during reproductive aging. High expression of NLRP3, caspase-1, and IL-1β was also observed in granulosa cells from patients with ovarian insufficiency. Ablation of NLRP3 improved the survival and pregnancy rates and increased anti-Müllerian hormone levels and autophagy rates in ovaries. Deficiency of NLRP3 also reduced serum FSH and estradiol levels. Consistent with these results, pharmacological inhibition of NLRP3 using a direct NLRP3 inhibitor, MCC950, improved fertility in female mice to levels comparable to those of Nlrp3−/− mice. These results suggest that the NLRP3 inflammasome is implicated in the age-dependent loss of female fertility and position this inflammasome as a potential new therapeutic target for the treatment of infertility. metadata Navarro-Pando, José Manuel; Alcocer-Gómez, Elísabet; Castejón-Vega, Beatriz; Navarro-Villarán, Elena; Condés-Hervás, Mónica; Mundi-Roldan, María; Muntané, Jordi; Pérez-Pulido, Antonio J.; Bullon, Pedro; Wang, Chun; Hoffman, Hal M.; Sanz, Alberto; Mbalaviele, Gabriel; Ryffel, Bernhard y Cordero, Mario D. mail jose.navarro@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, monica.condes@uneatlantico.es, maria.mundi@uneatlantico.es, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, SIN ESPECIFICAR, mario.cordero@uneatlantico.es (2021) Inhibition of the NLRP3 inflammasome prevents ovarian aging. Science Advances, 7 (1). eabc7409. ISSN 2375-2548 document_url: http://repositorio.uneatlantico.es/id/eprint/107/1/eabc7409.full.pdf