TY  - JOUR
UR  - http://doi.org/10.3233/JBR-230058
A1  - Castejón-Vega, Beatriz
A1  - Kyriakidis, Nikolaos C.
A1  - Alcócer-Gómez, Elizabet
A1  - Giampieri, Francesca
A1  - González-Paramás, Ana M.
A1  - Cordero, Mario D.
A1  - Alvarez-Suarez, José M.
JF  - Journal of Berry Research
TI  - Modulatory effect of Andean blackberry polyphenols on genes related to antioxidant and inflammatory responses, the NLRP3 inflammasome, and autophagy
EP  - 19
N2  - BACKGROUND: The Andean blackberry (Rubus glaucus Benth) is one of Ecuador?s most iconic Andean berries for which a high anthocyanin content has been described. OBJECTIVE: The aim of the present study was to determine the chemical composition and anti-inflammatory potential of the Andean blackberry from Ecuador, with an emphasis on its effects on NLRP3 inflammasome activation and autophagy processes. RESULTS: Andean blackberry extracts were rich in hydroxycinnamates (coumaric acid and derivates), in addition to quercetin and kaempferol as principal flavonols. Cyanidin and its glycosides were identified as the main anthocyanins present. Andean blackberry extracts efficiently reduced oxidative stress markers in the lipopolysaccharide-stimulated RAW 264.7 cells. The extracts also caused a moderate decrease in the expression of the pro-inflammatory and antioxidant genes NF?B1, TNF, IL-1?, IL-6, and NOS2 expression, while they significantly increased the mRNA levels of both SOD1 and NFE2L2 genes. Andean blackberry extracts significantly decreased the activation of the NLRP3 inflammasome complex, as well as p62 levels, and the LC3I/LC3II ratio increased, suggesting a direct action of Andean blackberry compounds on the inflammatory response and restoration of the autophagy process. CONCLUSIONS:These results suggest that Andean blackberries potentially have an anti-inflammatory effect through their ability to regulate genes related to the inflammatory and antioxidant response, as well as modulate the activation of the NLRP3 inflammasome complex and autophagy processes.
SN  - 18785093
ID  - uneatlantico10418
AV  - none
KW  - Anthocyanins
KW  -  autophagy
KW  -  blackberries
KW  -  inflammation
KW  -  NRLP3 inflammasome
SP  - 1
Y1  - 2023/12//
ER  -